This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.90 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 224,696 (7.1%) Reads that were too short: 20,994 (0.7%) Reads written (passing filters): 203,702 (6.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 54,759,039 bp (5.7%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 224696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1246 773.1 0 1246 7 77387 773.1 0 77387 8 1640 773.1 0 1640 9 86081 773.1 0 86081 10 162 773.1 0 162 11 62 773.1 0 62 12 93 773.1 0 93 13 63 773.1 0 63 14 53 773.1 0 53 15 70 773.1 0 70 16 66 773.1 0 66 17 66 773.1 0 66 18 50 773.1 0 50 19 66 773.1 0 66 20 81 773.1 0 81 21 71 773.1 0 71 22 73 773.1 0 73 23 79 773.1 0 79 24 84 773.1 0 84 25 77 773.1 0 77 26 62 773.1 0 62 27 78 773.1 0 78 28 81 773.1 0 81 29 97 773.1 0 97 30 79 773.1 0 79 31 219 773.1 0 219 32 327 773.1 0 327 33 296 773.1 0 296 34 69 773.1 0 69 35 60 773.1 0 60 36 59 773.1 0 59 37 87 773.1 0 87 38 71 773.1 0 71 39 68 773.1 0 68 40 59 773.1 0 59 41 78 773.1 0 78 42 56 773.1 0 56 43 63 773.1 0 63 44 61 773.1 0 61 45 61 773.1 0 61 46 85 773.1 0 85 47 63 773.1 0 63 48 74 773.1 0 74 49 58 773.1 0 58 50 92 773.1 0 92 51 94 773.1 0 94 52 120 773.1 0 120 53 56 773.1 0 56 54 52 773.1 0 52 55 72 773.1 0 72 56 68 773.1 0 68 57 51 773.1 0 51 58 71 773.1 0 71 59 74 773.1 0 74 60 51 773.1 0 51 61 49 773.1 0 49 62 73 773.1 0 73 63 73 773.1 0 73 64 72 773.1 0 72 65 79 773.1 0 79 66 78 773.1 0 78 67 94 773.1 0 94 68 415 773.1 0 415 69 552 773.1 0 552 70 762 773.1 0 762 71 51 773.1 0 51 72 71 773.1 0 71 73 93 773.1 0 93 74 88 773.1 0 88 75 80 773.1 0 80 76 52 773.1 0 52 77 61 773.1 0 61 78 64 773.1 0 64 79 119 773.1 0 119 80 98 773.1 0 98 81 87 773.1 0 87 82 124 773.1 0 124 83 88 773.1 0 88 84 96 773.1 0 96 85 68 773.1 0 68 86 87 773.1 0 87 87 78 773.1 0 78 88 101 773.1 0 101 89 80 773.1 0 80 90 63 773.1 0 63 91 68 773.1 0 68 92 72 773.1 0 72 93 73 773.1 0 73 94 80 773.1 0 80 95 63 773.1 0 63 96 62 773.1 0 62 97 70 773.1 0 70 98 148 773.1 0 148 99 114 773.1 0 114 100 140 773.1 0 140 101 81 773.1 0 81 102 76 773.1 0 76 103 53 773.1 0 53 104 63 773.1 0 63 105 55 773.1 0 55 106 67 773.1 0 67 107 74 773.1 0 74 108 66 773.1 0 66 109 70 773.1 0 70 110 85 773.1 0 85 111 76 773.1 0 76 112 78 773.1 0 78 113 89 773.1 0 89 114 76 773.1 0 76 115 67 773.1 0 67 116 387 773.1 0 387 117 253 773.1 0 253 118 147 773.1 0 147 119 60 773.1 0 60 120 101 773.1 0 101 121 182 773.1 0 182 122 167 773.1 0 167 123 181 773.1 0 181 124 128 773.1 0 128 125 92 773.1 0 92 126 97 773.1 0 97 127 102 773.1 0 102 128 106 773.1 0 106 129 128 773.1 0 128 130 141 773.1 0 141 131 193 773.1 0 193 132 155 773.1 0 155 133 745 773.1 0 745 134 810 773.1 0 810 135 1106 773.1 0 1106 136 355 773.1 0 355 137 220 773.1 0 220 138 234 773.1 0 234 139 320 773.1 0 320 140 62 773.1 0 62 141 88 773.1 0 88 142 150 773.1 0 150 143 80 773.1 0 80 144 136 773.1 0 136 145 119 773.1 0 119 146 183 773.1 0 183 147 193 773.1 0 193 148 143 773.1 0 143 149 87 773.1 0 87 150 179 773.1 0 179 151 356 773.1 0 356 152 659 773.1 0 659 153 312 773.1 0 312 154 364 773.1 0 364 155 260 773.1 0 260 156 195 773.1 0 195 157 212 773.1 0 212 158 104 773.1 0 104 159 111 773.1 0 111 160 231 773.1 0 231 161 80 773.1 0 80 162 140 773.1 0 140 163 111 773.1 0 111 164 133 773.1 0 133 165 176 773.1 0 176 166 101 773.1 0 101 167 243 773.1 0 243 168 164 773.1 0 164 169 153 773.1 0 153 170 102 773.1 0 102 171 80 773.1 0 80 172 67 773.1 0 67 173 77 773.1 0 77 174 180 773.1 0 180 175 588 773.1 0 588 176 443 773.1 0 443 177 605 773.1 0 605 178 217 773.1 0 217 179 201 773.1 0 201 180 851 773.1 0 851 181 1026 773.1 0 1026 182 1341 773.1 0 1341 183 418 773.1 0 418 184 144 773.1 0 144 185 96 773.1 0 96 186 89 773.1 0 89 187 139 773.1 0 139 188 120 773.1 0 120 189 103 773.1 0 103 190 94 773.1 0 94 191 80 773.1 0 80 192 108 773.1 0 108 193 133 773.1 0 133 194 214 773.1 0 214 195 711 773.1 0 711 196 863 773.1 0 863 197 758 773.1 0 758 198 286 773.1 0 286 199 137 773.1 0 137 200 1916 773.1 0 1916 201 3036 773.1 0 3036 202 2281 773.1 0 2281 203 692 773.1 0 692 204 59 773.1 0 59 205 74 773.1 0 74 206 62 773.1 0 62 207 88 773.1 0 88 208 132 773.1 0 132 209 76 773.1 0 76 210 86 773.1 0 86 211 63 773.1 0 63 212 85 773.1 0 85 213 114 773.1 0 114 214 91 773.1 0 91 215 90 773.1 0 90 216 77 773.1 0 77 217 73 773.1 0 73 218 91 773.1 0 91 219 77 773.1 0 77 220 79 773.1 0 79 221 324 773.1 0 324 222 295 773.1 0 295 223 212 773.1 0 212 224 124 773.1 0 124 225 88 773.1 0 88 226 100 773.1 0 100 227 105 773.1 0 105 228 61 773.1 0 61 229 61 773.1 0 61 230 77 773.1 0 77 231 82 773.1 0 82 232 76 773.1 0 76 233 93 773.1 0 93 234 64 773.1 0 64 235 93 773.1 0 93 236 94 773.1 0 94 237 84 773.1 0 84 238 84 773.1 0 84 239 149 773.1 0 149 240 121 773.1 0 121 241 180 773.1 0 180 242 200 773.1 0 200 243 133 773.1 0 133 244 102 773.1 0 102 245 80 773.1 0 80 246 68 773.1 0 68 247 75 773.1 0 75 248 104 773.1 0 104 249 307 773.1 0 307 250 262 773.1 0 262 251 423 773.1 0 423 252 151 773.1 0 151 253 266 773.1 0 266 254 225 773.1 0 225 255 295 773.1 0 295 256 201 773.1 0 201 257 205 773.1 0 205 258 409 773.1 0 409 259 228 773.1 0 228 260 369 773.1 0 369 261 136 773.1 0 136 262 159 773.1 0 159 263 256 773.1 0 256 264 1070 773.1 0 1070 265 959 773.1 0 959 266 989 773.1 0 989 267 114 773.1 0 114 268 149 773.1 0 149 269 164 773.1 0 164 270 105 773.1 0 105 271 87 773.1 0 87 272 102 773.1 0 102 273 148 773.1 0 148 274 188 773.1 0 188 275 257 773.1 0 257 276 902 773.1 0 902 277 712 773.1 0 712 278 892 773.1 0 892 279 64 773.1 0 64 280 79 773.1 0 79 281 53 773.1 0 53 282 70 773.1 0 70 283 89 773.1 0 89 284 68 773.1 0 68 285 84 773.1 0 84 286 86 773.1 0 86 287 88 773.1 0 88 288 75 773.1 0 75 289 104 773.1 0 104 290 144 773.1 0 144 291 131 773.1 0 131 292 126 773.1 0 126 293 86 773.1 0 86 294 120 773.1 0 120 295 105 773.1 0 105 296 116 773.1 0 116 297 127 773.1 0 127 298 156 773.1 0 156 299 123 773.1 0 123 300 162 773.1 0 162 301 289 773.1 0 289 Finished in 14.35 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 203,702 Reads with adapters: 89,137 (43.8%) Reads that were too short: 180 (0.1%) Reads written (passing filters): 88,957 (43.7%) Total basepairs processed: 54,759,039 bp Total written (filtered): 24,434,599 bp (44.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 89137 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 13 0.0 2 0 0 0 13 15 118 0.0 3 0 0 99 19 16 1081 0.0 3 0 955 89 37 17 87055 0.0 3 82058 4040 750 207 18 246 0.0 3 30 176 20 20 19 201 0.0 3 3 0 133 65 20 48 0.0 3 0 1 0 47 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 7 0.0 3 0 0 0 7 29 2 0.0 3 0 0 1 1 34 2 0.0 3 1 0 0 1 36 25 0.0 3 1 0 0 24 37 2 0.0 3 0 0 0 2 39 6 0.0 3 0 0 1 5 42 10 0.0 3 0 0 0 10 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 6 0.0 3 0 0 0 6 54 1 0.0 3 0 0 0 1 55 7 0.0 3 0 0 0 7 57 43 0.0 3 0 0 0 43 58 3 0.0 3 0 0 0 3 59 10 0.0 3 0 0 0 10 60 5 0.0 3 0 0 0 5 61 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 75 15 0.0 3 0 0 0 15 76 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 87 2 0.0 3 0 0 0 2 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 93 9 0.0 3 0 0 0 9 94 2 0.0 3 0 0 1 1 99 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 8 0.0 3 0 0 0 8 104 2 0.0 3 0 0 0 2 105 3 0.0 3 0 0 0 3 106 7 0.0 3 0 0 1 6 107 3 0.0 3 0 0 0 3 108 3 0.0 3 0 0 0 3 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 4 0.0 3 0 0 0 4 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 121 5 0.0 3 0 0 0 5 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 153 12 0.0 3 0 0 2 10 156 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 13 0.0 3 0 0 0 13 162 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 197 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 1 1 204 1 0.0 3 0 0 0 1 205 8 0.0 3 0 0 0 8 206 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.91 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 88,957 Reads with adapters: 1,957 (2.2%) Reads written (passing filters): 88,957 (100.0%) Total basepairs processed: 24,434,599 bp Total written (filtered): 24,428,681 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1957 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.0% C: 13.8% G: 11.0% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1913 1390.0 0 1913 4 41 347.5 0 41 5 3 86.9 0 3