This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 145.58 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 213,254 (6.7%) Reads that were too short: 14,172 (0.4%) Reads written (passing filters): 199,082 (6.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 54,952,894 bp (5.8%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 213254 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 929 773.1 0 929 7 96721 773.1 0 96721 8 892 773.1 0 892 9 74089 773.1 0 74089 10 120 773.1 0 120 11 50 773.1 0 50 12 49 773.1 0 49 13 60 773.1 0 60 14 33 773.1 0 33 15 53 773.1 0 53 16 56 773.1 0 56 17 53 773.1 0 53 18 33 773.1 0 33 19 38 773.1 0 38 20 52 773.1 0 52 21 74 773.1 0 74 22 43 773.1 0 43 23 47 773.1 0 47 24 34 773.1 0 34 25 34 773.1 0 34 26 55 773.1 0 55 27 50 773.1 0 50 28 40 773.1 0 40 29 82 773.1 0 82 30 74 773.1 0 74 31 81 773.1 0 81 32 83 773.1 0 83 33 73 773.1 0 73 34 45 773.1 0 45 35 57 773.1 0 57 36 45 773.1 0 45 37 71 773.1 0 71 38 70 773.1 0 70 39 56 773.1 0 56 40 48 773.1 0 48 41 60 773.1 0 60 42 64 773.1 0 64 43 46 773.1 0 46 44 62 773.1 0 62 45 41 773.1 0 41 46 58 773.1 0 58 47 61 773.1 0 61 48 50 773.1 0 50 49 51 773.1 0 51 50 63 773.1 0 63 51 58 773.1 0 58 52 78 773.1 0 78 53 47 773.1 0 47 54 47 773.1 0 47 55 44 773.1 0 44 56 49 773.1 0 49 57 50 773.1 0 50 58 53 773.1 0 53 59 53 773.1 0 53 60 48 773.1 0 48 61 46 773.1 0 46 62 44 773.1 0 44 63 62 773.1 0 62 64 52 773.1 0 52 65 78 773.1 0 78 66 63 773.1 0 63 67 72 773.1 0 72 68 438 773.1 0 438 69 615 773.1 0 615 70 832 773.1 0 832 71 40 773.1 0 40 72 58 773.1 0 58 73 44 773.1 0 44 74 42 773.1 0 42 75 44 773.1 0 44 76 37 773.1 0 37 77 45 773.1 0 45 78 61 773.1 0 61 79 55 773.1 0 55 80 69 773.1 0 69 81 66 773.1 0 66 82 117 773.1 0 117 83 66 773.1 0 66 84 65 773.1 0 65 85 58 773.1 0 58 86 50 773.1 0 50 87 58 773.1 0 58 88 90 773.1 0 90 89 62 773.1 0 62 90 42 773.1 0 42 91 62 773.1 0 62 92 55 773.1 0 55 93 53 773.1 0 53 94 39 773.1 0 39 95 63 773.1 0 63 96 50 773.1 0 50 97 46 773.1 0 46 98 101 773.1 0 101 99 87 773.1 0 87 100 73 773.1 0 73 101 75 773.1 0 75 102 62 773.1 0 62 103 69 773.1 0 69 104 52 773.1 0 52 105 48 773.1 0 48 106 47 773.1 0 47 107 54 773.1 0 54 108 62 773.1 0 62 109 47 773.1 0 47 110 70 773.1 0 70 111 45 773.1 0 45 112 45 773.1 0 45 113 52 773.1 0 52 114 55 773.1 0 55 115 53 773.1 0 53 116 142 773.1 0 142 117 128 773.1 0 128 118 106 773.1 0 106 119 68 773.1 0 68 120 69 773.1 0 69 121 109 773.1 0 109 122 95 773.1 0 95 123 102 773.1 0 102 124 127 773.1 0 127 125 67 773.1 0 67 126 75 773.1 0 75 127 67 773.1 0 67 128 92 773.1 0 92 129 82 773.1 0 82 130 105 773.1 0 105 131 120 773.1 0 120 132 92 773.1 0 92 133 601 773.1 0 601 134 571 773.1 0 571 135 851 773.1 0 851 136 240 773.1 0 240 137 194 773.1 0 194 138 259 773.1 0 259 139 323 773.1 0 323 140 66 773.1 0 66 141 57 773.1 0 57 142 97 773.1 0 97 143 68 773.1 0 68 144 93 773.1 0 93 145 85 773.1 0 85 146 136 773.1 0 136 147 104 773.1 0 104 148 111 773.1 0 111 149 65 773.1 0 65 150 125 773.1 0 125 151 303 773.1 0 303 152 571 773.1 0 571 153 306 773.1 0 306 154 287 773.1 0 287 155 118 773.1 0 118 156 141 773.1 0 141 157 128 773.1 0 128 158 77 773.1 0 77 159 120 773.1 0 120 160 231 773.1 0 231 161 72 773.1 0 72 162 108 773.1 0 108 163 114 773.1 0 114 164 116 773.1 0 116 165 143 773.1 0 143 166 96 773.1 0 96 167 136 773.1 0 136 168 109 773.1 0 109 169 107 773.1 0 107 170 63 773.1 0 63 171 56 773.1 0 56 172 75 773.1 0 75 173 61 773.1 0 61 174 134 773.1 0 134 175 396 773.1 0 396 176 232 773.1 0 232 177 348 773.1 0 348 178 116 773.1 0 116 179 114 773.1 0 114 180 680 773.1 0 680 181 714 773.1 0 714 182 1129 773.1 0 1129 183 304 773.1 0 304 184 100 773.1 0 100 185 71 773.1 0 71 186 65 773.1 0 65 187 92 773.1 0 92 188 73 773.1 0 73 189 70 773.1 0 70 190 74 773.1 0 74 191 72 773.1 0 72 192 80 773.1 0 80 193 70 773.1 0 70 194 175 773.1 0 175 195 368 773.1 0 368 196 475 773.1 0 475 197 390 773.1 0 390 198 194 773.1 0 194 199 66 773.1 0 66 200 1172 773.1 0 1172 201 1669 773.1 0 1669 202 1440 773.1 0 1440 203 444 773.1 0 444 204 56 773.1 0 56 205 55 773.1 0 55 206 61 773.1 0 61 207 59 773.1 0 59 208 139 773.1 0 139 209 71 773.1 0 71 210 58 773.1 0 58 211 56 773.1 0 56 212 63 773.1 0 63 213 107 773.1 0 107 214 65 773.1 0 65 215 55 773.1 0 55 216 58 773.1 0 58 217 49 773.1 0 49 218 58 773.1 0 58 219 66 773.1 0 66 220 63 773.1 0 63 221 149 773.1 0 149 222 140 773.1 0 140 223 107 773.1 0 107 224 85 773.1 0 85 225 59 773.1 0 59 226 87 773.1 0 87 227 82 773.1 0 82 228 71 773.1 0 71 229 48 773.1 0 48 230 65 773.1 0 65 231 65 773.1 0 65 232 69 773.1 0 69 233 62 773.1 0 62 234 61 773.1 0 61 235 67 773.1 0 67 236 68 773.1 0 68 237 68 773.1 0 68 238 73 773.1 0 73 239 154 773.1 0 154 240 78 773.1 0 78 241 139 773.1 0 139 242 112 773.1 0 112 243 86 773.1 0 86 244 71 773.1 0 71 245 69 773.1 0 69 246 62 773.1 0 62 247 71 773.1 0 71 248 68 773.1 0 68 249 163 773.1 0 163 250 145 773.1 0 145 251 244 773.1 0 244 252 89 773.1 0 89 253 235 773.1 0 235 254 200 773.1 0 200 255 215 773.1 0 215 256 143 773.1 0 143 257 128 773.1 0 128 258 417 773.1 0 417 259 264 773.1 0 264 260 343 773.1 0 343 261 94 773.1 0 94 262 100 773.1 0 100 263 140 773.1 0 140 264 536 773.1 0 536 265 538 773.1 0 538 266 465 773.1 0 465 267 78 773.1 0 78 268 87 773.1 0 87 269 85 773.1 0 85 270 76 773.1 0 76 271 60 773.1 0 60 272 77 773.1 0 77 273 76 773.1 0 76 274 96 773.1 0 96 275 114 773.1 0 114 276 522 773.1 0 522 277 480 773.1 0 480 278 724 773.1 0 724 279 36 773.1 0 36 280 60 773.1 0 60 281 49 773.1 0 49 282 50 773.1 0 50 283 65 773.1 0 65 284 49 773.1 0 49 285 73 773.1 0 73 286 81 773.1 0 81 287 72 773.1 0 72 288 58 773.1 0 58 289 61 773.1 0 61 290 108 773.1 0 108 291 63 773.1 0 63 292 72 773.1 0 72 293 66 773.1 0 66 294 91 773.1 0 91 295 88 773.1 0 88 296 80 773.1 0 80 297 91 773.1 0 91 298 135 773.1 0 135 299 127 773.1 0 127 300 158 773.1 0 158 301 146 773.1 0 146 Finished in 14.91 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 199,082 Reads with adapters: 96,786 (48.6%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 96,758 (48.6%) Total basepairs processed: 54,952,894 bp Total written (filtered): 26,495,918 bp (48.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 96786 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 5 0.0 3 0 0 3 2 19 228 0.0 3 0 216 8 1 3 20 96113 0.0 4 94576 1496 32 5 4 21 188 0.0 4 33 152 3 22 11 0.0 4 2 0 9 23 59 0.0 4 0 0 0 52 7 24 4 0.0 4 0 0 0 0 4 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 1 35 1 0.0 4 0 1 36 1 0.0 4 1 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 59 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 72 1 0.0 4 1 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 1 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 134 0.0 4 0 0 0 0 134 136 1 0.0 4 0 0 0 0 1 169 1 0.0 4 1 171 1 0.0 4 0 0 0 0 1 180 2 0.0 4 0 0 0 2 193 1 0.0 4 1 196 1 0.0 4 1 207 1 0.0 4 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 276 1 0.0 4 0 0 0 1 278 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.39 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 96,758 Reads with adapters: 3,819 (3.9%) Reads written (passing filters): 96,758 (100.0%) Total basepairs processed: 26,495,918 bp Total written (filtered): 26,452,180 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3819 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 2.2% G: 93.9% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 90 1511.8 0 90 4 10 378.0 0 10 5 3 94.5 0 3 6 1 23.6 0 1 7 1 5.9 0 1 8 1 1.5 0 1 9 4 0.4 0 3 1 10 12 0.1 1 0 12 11 3580 0.0 1 21 3559 12 91 0.0 1 6 85 13 3 0.0 1 0 3 15 4 0.0 1 0 4 16 1 0.0 1 0 1 21 1 0.0 1 0 1 45 1 0.0 1 0 1 79 1 0.0 1 0 1 114 1 0.0 1 1 149 2 0.0 1 1 1 152 5 0.0 1 0 5 157 1 0.0 1 1 163 1 0.0 1 1 176 1 0.0 1 1 191 1 0.0 1 0 1 210 2 0.0 1 2 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.