This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 145.84 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 223,583 (7.1%) Reads that were too short: 27,783 (0.9%) Reads written (passing filters): 195,800 (6.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 53,991,925 bp (5.7%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 223583 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 866 773.1 0 866 7 29908 773.1 0 29908 8 925 773.1 0 925 9 23533 773.1 0 23533 10 899 773.1 0 899 11 908 773.1 0 908 12 16960 773.1 0 16960 13 21640 773.1 0 21640 14 77669 773.1 0 77669 15 158 773.1 0 158 16 43 773.1 0 43 17 36 773.1 0 36 18 33 773.1 0 33 19 44 773.1 0 44 20 30 773.1 0 30 21 34 773.1 0 34 22 45 773.1 0 45 23 41 773.1 0 41 24 28 773.1 0 28 25 28 773.1 0 28 26 75 773.1 0 75 27 111 773.1 0 111 28 74 773.1 0 74 29 39 773.1 0 39 30 40 773.1 0 40 31 29 773.1 0 29 32 43 773.1 0 43 33 38 773.1 0 38 34 35 773.1 0 35 35 41 773.1 0 41 36 33 773.1 0 33 37 36 773.1 0 36 38 44 773.1 0 44 39 40 773.1 0 40 40 62 773.1 0 62 41 42 773.1 0 42 42 56 773.1 0 56 43 90 773.1 0 90 44 144 773.1 0 144 45 135 773.1 0 135 46 35 773.1 0 35 47 30 773.1 0 30 48 45 773.1 0 45 49 50 773.1 0 50 50 40 773.1 0 40 51 25 773.1 0 25 52 34 773.1 0 34 53 40 773.1 0 40 54 40 773.1 0 40 55 26 773.1 0 26 56 42 773.1 0 42 57 55 773.1 0 55 58 51 773.1 0 51 59 54 773.1 0 54 60 29 773.1 0 29 61 40 773.1 0 40 62 40 773.1 0 40 63 46 773.1 0 46 64 39 773.1 0 39 65 38 773.1 0 38 66 46 773.1 0 46 67 49 773.1 0 49 68 34 773.1 0 34 69 36 773.1 0 36 70 39 773.1 0 39 71 34 773.1 0 34 72 26 773.1 0 26 73 59 773.1 0 59 74 42 773.1 0 42 75 39 773.1 0 39 76 39 773.1 0 39 77 40 773.1 0 40 78 71 773.1 0 71 79 65 773.1 0 65 80 52 773.1 0 52 81 42 773.1 0 42 82 36 773.1 0 36 83 36 773.1 0 36 84 49 773.1 0 49 85 49 773.1 0 49 86 44 773.1 0 44 87 53 773.1 0 53 88 36 773.1 0 36 89 61 773.1 0 61 90 54 773.1 0 54 91 47 773.1 0 47 92 125 773.1 0 125 93 39 773.1 0 39 94 56 773.1 0 56 95 42 773.1 0 42 96 51 773.1 0 51 97 39 773.1 0 39 98 77 773.1 0 77 99 54 773.1 0 54 100 30 773.1 0 30 101 46 773.1 0 46 102 57 773.1 0 57 103 51 773.1 0 51 104 361 773.1 0 361 105 306 773.1 0 306 106 557 773.1 0 557 107 65 773.1 0 65 108 126 773.1 0 126 109 70 773.1 0 70 110 124 773.1 0 124 111 97 773.1 0 97 112 113 773.1 0 113 113 118 773.1 0 118 114 79 773.1 0 79 115 76 773.1 0 76 116 81 773.1 0 81 117 85 773.1 0 85 118 39 773.1 0 39 119 49 773.1 0 49 120 76 773.1 0 76 121 85 773.1 0 85 122 66 773.1 0 66 123 72 773.1 0 72 124 111 773.1 0 111 125 300 773.1 0 300 126 554 773.1 0 554 127 452 773.1 0 452 128 462 773.1 0 462 129 198 773.1 0 198 130 65 773.1 0 65 131 52 773.1 0 52 132 64 773.1 0 64 133 89 773.1 0 89 134 105 773.1 0 105 135 148 773.1 0 148 136 117 773.1 0 117 137 371 773.1 0 371 138 541 773.1 0 541 139 810 773.1 0 810 140 978 773.1 0 978 141 464 773.1 0 464 142 513 773.1 0 513 143 75 773.1 0 75 144 48 773.1 0 48 145 68 773.1 0 68 146 78 773.1 0 78 147 104 773.1 0 104 148 49 773.1 0 49 149 52 773.1 0 52 150 59 773.1 0 59 151 259 773.1 0 259 152 242 773.1 0 242 153 249 773.1 0 249 154 90 773.1 0 90 155 101 773.1 0 101 156 99 773.1 0 99 157 129 773.1 0 129 158 1208 773.1 0 1208 159 1081 773.1 0 1081 160 1121 773.1 0 1121 161 353 773.1 0 353 162 108 773.1 0 108 163 145 773.1 0 145 164 61 773.1 0 61 165 65 773.1 0 65 166 54 773.1 0 54 167 94 773.1 0 94 168 76 773.1 0 76 169 131 773.1 0 131 170 166 773.1 0 166 171 101 773.1 0 101 172 40 773.1 0 40 173 70 773.1 0 70 174 92 773.1 0 92 175 101 773.1 0 101 176 89 773.1 0 89 177 76 773.1 0 76 178 40 773.1 0 40 179 63 773.1 0 63 180 71 773.1 0 71 181 171 773.1 0 171 182 193 773.1 0 193 183 169 773.1 0 169 184 97 773.1 0 97 185 53 773.1 0 53 186 62 773.1 0 62 187 73 773.1 0 73 188 204 773.1 0 204 189 121 773.1 0 121 190 124 773.1 0 124 191 42 773.1 0 42 192 60 773.1 0 60 193 49 773.1 0 49 194 61 773.1 0 61 195 40 773.1 0 40 196 58 773.1 0 58 197 59 773.1 0 59 198 63 773.1 0 63 199 89 773.1 0 89 200 97 773.1 0 97 201 89 773.1 0 89 202 83 773.1 0 83 203 66 773.1 0 66 204 60 773.1 0 60 205 55 773.1 0 55 206 74 773.1 0 74 207 107 773.1 0 107 208 113 773.1 0 113 209 80 773.1 0 80 210 46 773.1 0 46 211 72 773.1 0 72 212 117 773.1 0 117 213 99 773.1 0 99 214 122 773.1 0 122 215 155 773.1 0 155 216 123 773.1 0 123 217 65 773.1 0 65 218 86 773.1 0 86 219 98 773.1 0 98 220 490 773.1 0 490 221 331 773.1 0 331 222 279 773.1 0 279 223 183 773.1 0 183 224 1290 773.1 0 1290 225 667 773.1 0 667 226 3695 773.1 0 3695 227 94 773.1 0 94 228 69 773.1 0 69 229 52 773.1 0 52 230 92 773.1 0 92 231 111 773.1 0 111 232 165 773.1 0 165 233 295 773.1 0 295 234 278 773.1 0 278 235 216 773.1 0 216 236 162 773.1 0 162 237 98 773.1 0 98 238 62 773.1 0 62 239 66 773.1 0 66 240 97 773.1 0 97 241 133 773.1 0 133 242 178 773.1 0 178 243 90 773.1 0 90 244 80 773.1 0 80 245 59 773.1 0 59 246 36 773.1 0 36 247 68 773.1 0 68 248 47 773.1 0 47 249 41 773.1 0 41 250 64 773.1 0 64 251 88 773.1 0 88 252 89 773.1 0 89 253 95 773.1 0 95 254 101 773.1 0 101 255 71 773.1 0 71 256 102 773.1 0 102 257 88 773.1 0 88 258 107 773.1 0 107 259 52 773.1 0 52 260 97 773.1 0 97 261 94 773.1 0 94 262 106 773.1 0 106 263 84 773.1 0 84 264 66 773.1 0 66 265 78 773.1 0 78 266 65 773.1 0 65 267 361 773.1 0 361 268 337 773.1 0 337 269 391 773.1 0 391 270 141 773.1 0 141 271 105 773.1 0 105 272 130 773.1 0 130 273 111 773.1 0 111 274 102 773.1 0 102 275 77 773.1 0 77 276 151 773.1 0 151 277 356 773.1 0 356 278 431 773.1 0 431 279 405 773.1 0 405 280 691 773.1 0 691 281 2377 773.1 0 2377 282 2848 773.1 0 2848 283 2959 773.1 0 2959 284 883 773.1 0 883 285 62 773.1 0 62 286 90 773.1 0 90 287 122 773.1 0 122 288 340 773.1 0 340 289 271 773.1 0 271 290 418 773.1 0 418 291 121 773.1 0 121 292 96 773.1 0 96 293 84 773.1 0 84 294 116 773.1 0 116 295 161 773.1 0 161 296 147 773.1 0 147 297 74 773.1 0 74 298 58 773.1 0 58 299 36 773.1 0 36 300 60 773.1 0 60 301 79 773.1 0 79 Finished in 13.11 s (67 us/read; 0.90 M reads/minute). === Summary === Total reads processed: 195,800 Reads with adapters: 32,053 (16.4%) Reads that were too short: 366 (0.2%) Reads written (passing filters): 31,687 (16.2%) Total basepairs processed: 53,991,925 bp Total written (filtered): 8,463,468 bp (15.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32053 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 6 0.0 3 0 0 3 3 16 244 0.0 3 0 220 17 7 17 28953 0.0 3 27558 1175 178 42 18 94 0.0 3 15 59 10 10 19 71 0.0 3 0 1 51 19 20 244 0.0 3 181 5 6 52 21 2 0.0 3 1 0 0 1 22 1 0.0 3 1 24 1 0.0 3 0 0 1 25 1 0.0 3 0 1 26 1 0.0 3 1 27 1 0.0 3 1 30 1 0.0 3 1 31 1 0.0 3 1 32 19 0.0 3 0 0 1 18 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 8 0.0 3 0 0 0 8 39 3 0.0 3 0 0 0 3 40 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 50 0.0 3 0 0 1 49 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 11 0.0 3 0 0 1 10 57 3 0.0 3 0 0 0 3 58 15 0.0 3 0 0 0 15 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 4 0.0 3 0 0 0 4 65 1 0.0 3 0 0 0 1 67 45 0.0 3 0 0 0 45 69 1 0.0 3 0 0 0 1 70 10 0.0 3 0 0 0 10 71 3 0.0 3 0 0 1 2 73 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 3 0.0 3 0 0 0 3 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 5 0.0 3 0 0 0 5 90 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 1 2 110 54 0.0 3 0 0 1 53 111 8 0.0 3 0 0 0 8 112 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 138 14 0.0 3 0 0 0 14 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 19 0.0 3 0 0 0 19 155 5 0.0 3 0 0 0 5 156 4 0.0 3 0 0 0 4 157 6 0.0 3 0 0 1 5 158 15 0.0 3 0 0 0 15 159 958 0.0 3 0 0 12 946 160 67 0.0 3 0 0 0 67 161 9 0.0 3 0 0 0 9 162 127 0.0 3 0 0 9 118 163 18 0.0 3 0 0 1 17 164 7 0.0 3 0 0 1 6 166 1 0.0 3 1 167 17 0.0 3 0 0 0 17 168 2 0.0 3 0 0 1 1 169 5 0.0 3 0 0 0 5 170 4 0.0 3 0 0 0 4 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 41 0.0 3 0 0 1 40 174 4 0.0 3 0 0 0 4 175 7 0.0 3 0 0 1 6 176 18 0.0 3 0 0 0 18 177 2 0.0 3 0 0 0 2 178 28 0.0 3 0 0 0 28 179 256 0.0 3 0 0 2 254 180 83 0.0 3 0 0 0 83 181 5 0.0 3 0 0 0 5 182 32 0.0 3 0 0 0 32 183 12 0.0 3 0 0 0 12 184 3 0.0 3 0 0 0 3 185 20 0.0 3 0 0 0 20 186 3 0.0 3 0 0 0 3 188 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 199 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 203 4 0.0 3 0 0 0 4 204 2 0.0 3 0 0 0 2 205 12 0.0 3 0 0 0 12 206 1 0.0 3 0 0 0 1 210 10 0.0 3 0 0 0 10 211 4 0.0 3 0 0 0 4 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 223 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 230 10 0.0 3 0 0 0 10 231 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 247 3 0.0 3 0 0 0 3 250 1 0.0 3 0 0 0 1 251 2 0.0 3 0 0 0 2 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 2 0.0 3 0 0 0 2 257 2 0.0 3 0 0 0 2 259 3 0.0 3 0 0 0 3 261 5 0.0 3 0 0 0 5 262 9 0.0 3 0 0 0 9 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 29 0.0 3 0 0 0 29 272 12 0.0 3 0 0 0 12 273 14 0.0 3 0 0 0 14 274 18 0.0 3 0 0 1 17 275 12 0.0 3 0 0 0 12 276 6 0.0 3 0 0 1 5 278 2 0.0 3 0 0 0 2 279 15 0.0 3 0 0 0 15 280 29 0.0 3 0 0 0 29 281 30 0.0 3 0 0 0 30 282 2 0.0 3 0 0 0 2 283 3 0.0 3 0 0 0 3 285 9 0.0 3 0 0 0 9 286 4 0.0 3 0 0 0 4 287 7 0.0 3 0 0 1 6 288 2 0.0 3 0 0 0 2 289 11 0.0 3 0 0 0 11 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.75 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 31,687 Reads with adapters: 327 (1.0%) Reads written (passing filters): 31,687 (100.0%) Total basepairs processed: 8,463,468 bp Total written (filtered): 8,462,469 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 327 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.3% C: 43.4% G: 31.2% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 314 495.1 0 314 4 10 123.8 0 10 5 1 30.9 0 1 6 2 7.7 0 2