This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 146.78 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 193,518 (6.1%) Reads that were too short: 18,674 (0.6%) Reads written (passing filters): 174,844 (5.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 48,887,790 bp (5.1%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 193518 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 778 773.1 0 778 7 25028 773.1 0 25028 8 502 773.1 0 502 9 30099 773.1 0 30099 10 708 773.1 0 708 11 694 773.1 0 694 12 14704 773.1 0 14704 13 19845 773.1 0 19845 14 67687 773.1 0 67687 15 109 773.1 0 109 16 23 773.1 0 23 17 26 773.1 0 26 18 31 773.1 0 31 19 18 773.1 0 18 20 27 773.1 0 27 21 27 773.1 0 27 22 18 773.1 0 18 23 26 773.1 0 26 24 35 773.1 0 35 25 36 773.1 0 36 26 31 773.1 0 31 27 27 773.1 0 27 28 22 773.1 0 22 29 31 773.1 0 31 30 20 773.1 0 20 31 21 773.1 0 21 32 32 773.1 0 32 33 27 773.1 0 27 34 19 773.1 0 19 35 28 773.1 0 28 36 19 773.1 0 19 37 24 773.1 0 24 38 28 773.1 0 28 39 37 773.1 0 37 40 40 773.1 0 40 41 29 773.1 0 29 42 20 773.1 0 20 43 59 773.1 0 59 44 92 773.1 0 92 45 120 773.1 0 120 46 24 773.1 0 24 47 20 773.1 0 20 48 35 773.1 0 35 49 25 773.1 0 25 50 30 773.1 0 30 51 32 773.1 0 32 52 21 773.1 0 21 53 43 773.1 0 43 54 33 773.1 0 33 55 19 773.1 0 19 56 15 773.1 0 15 57 33 773.1 0 33 58 39 773.1 0 39 59 25 773.1 0 25 60 20 773.1 0 20 61 27 773.1 0 27 62 23 773.1 0 23 63 37 773.1 0 37 64 30 773.1 0 30 65 24 773.1 0 24 66 37 773.1 0 37 67 35 773.1 0 35 68 28 773.1 0 28 69 21 773.1 0 21 70 38 773.1 0 38 71 26 773.1 0 26 72 32 773.1 0 32 73 37 773.1 0 37 74 33 773.1 0 33 75 24 773.1 0 24 76 36 773.1 0 36 77 33 773.1 0 33 78 47 773.1 0 47 79 42 773.1 0 42 80 35 773.1 0 35 81 34 773.1 0 34 82 48 773.1 0 48 83 45 773.1 0 45 84 32 773.1 0 32 85 52 773.1 0 52 86 43 773.1 0 43 87 33 773.1 0 33 88 44 773.1 0 44 89 57 773.1 0 57 90 52 773.1 0 52 91 45 773.1 0 45 92 104 773.1 0 104 93 40 773.1 0 40 94 61 773.1 0 61 95 50 773.1 0 50 96 40 773.1 0 40 97 40 773.1 0 40 98 51 773.1 0 51 99 40 773.1 0 40 100 39 773.1 0 39 101 29 773.1 0 29 102 44 773.1 0 44 103 47 773.1 0 47 104 257 773.1 0 257 105 215 773.1 0 215 106 390 773.1 0 390 107 52 773.1 0 52 108 63 773.1 0 63 109 52 773.1 0 52 110 93 773.1 0 93 111 65 773.1 0 65 112 65 773.1 0 65 113 85 773.1 0 85 114 61 773.1 0 61 115 48 773.1 0 48 116 64 773.1 0 64 117 52 773.1 0 52 118 35 773.1 0 35 119 46 773.1 0 46 120 66 773.1 0 66 121 55 773.1 0 55 122 58 773.1 0 58 123 41 773.1 0 41 124 78 773.1 0 78 125 133 773.1 0 133 126 420 773.1 0 420 127 341 773.1 0 341 128 376 773.1 0 376 129 161 773.1 0 161 130 54 773.1 0 54 131 44 773.1 0 44 132 53 773.1 0 53 133 56 773.1 0 56 134 74 773.1 0 74 135 133 773.1 0 133 136 85 773.1 0 85 137 315 773.1 0 315 138 180 773.1 0 180 139 376 773.1 0 376 140 514 773.1 0 514 141 325 773.1 0 325 142 368 773.1 0 368 143 58 773.1 0 58 144 41 773.1 0 41 145 59 773.1 0 59 146 56 773.1 0 56 147 50 773.1 0 50 148 49 773.1 0 49 149 41 773.1 0 41 150 48 773.1 0 48 151 244 773.1 0 244 152 197 773.1 0 197 153 180 773.1 0 180 154 45 773.1 0 45 155 70 773.1 0 70 156 68 773.1 0 68 157 70 773.1 0 70 158 656 773.1 0 656 159 541 773.1 0 541 160 633 773.1 0 633 161 188 773.1 0 188 162 57 773.1 0 57 163 69 773.1 0 69 164 48 773.1 0 48 165 53 773.1 0 53 166 44 773.1 0 44 167 54 773.1 0 54 168 45 773.1 0 45 169 63 773.1 0 63 170 142 773.1 0 142 171 114 773.1 0 114 172 38 773.1 0 38 173 36 773.1 0 36 174 72 773.1 0 72 175 65 773.1 0 65 176 54 773.1 0 54 177 37 773.1 0 37 178 38 773.1 0 38 179 54 773.1 0 54 180 35 773.1 0 35 181 99 773.1 0 99 182 65 773.1 0 65 183 61 773.1 0 61 184 57 773.1 0 57 185 45 773.1 0 45 186 59 773.1 0 59 187 57 773.1 0 57 188 102 773.1 0 102 189 76 773.1 0 76 190 60 773.1 0 60 191 36 773.1 0 36 192 38 773.1 0 38 193 42 773.1 0 42 194 50 773.1 0 50 195 44 773.1 0 44 196 38 773.1 0 38 197 51 773.1 0 51 198 55 773.1 0 55 199 56 773.1 0 56 200 50 773.1 0 50 201 66 773.1 0 66 202 54 773.1 0 54 203 63 773.1 0 63 204 50 773.1 0 50 205 36 773.1 0 36 206 45 773.1 0 45 207 67 773.1 0 67 208 66 773.1 0 66 209 67 773.1 0 67 210 47 773.1 0 47 211 42 773.1 0 42 212 100 773.1 0 100 213 36 773.1 0 36 214 128 773.1 0 128 215 117 773.1 0 117 216 98 773.1 0 98 217 74 773.1 0 74 218 76 773.1 0 76 219 68 773.1 0 68 220 310 773.1 0 310 221 247 773.1 0 247 222 234 773.1 0 234 223 172 773.1 0 172 224 1354 773.1 0 1354 225 593 773.1 0 593 226 3960 773.1 0 3960 227 73 773.1 0 73 228 38 773.1 0 38 229 46 773.1 0 46 230 46 773.1 0 46 231 48 773.1 0 48 232 108 773.1 0 108 233 202 773.1 0 202 234 255 773.1 0 255 235 200 773.1 0 200 236 112 773.1 0 112 237 81 773.1 0 81 238 47 773.1 0 47 239 49 773.1 0 49 240 79 773.1 0 79 241 97 773.1 0 97 242 118 773.1 0 118 243 70 773.1 0 70 244 69 773.1 0 69 245 45 773.1 0 45 246 33 773.1 0 33 247 49 773.1 0 49 248 43 773.1 0 43 249 51 773.1 0 51 250 48 773.1 0 48 251 79 773.1 0 79 252 71 773.1 0 71 253 67 773.1 0 67 254 46 773.1 0 46 255 48 773.1 0 48 256 74 773.1 0 74 257 84 773.1 0 84 258 71 773.1 0 71 259 51 773.1 0 51 260 63 773.1 0 63 261 69 773.1 0 69 262 78 773.1 0 78 263 77 773.1 0 77 264 54 773.1 0 54 265 52 773.1 0 52 266 60 773.1 0 60 267 203 773.1 0 203 268 254 773.1 0 254 269 245 773.1 0 245 270 92 773.1 0 92 271 85 773.1 0 85 272 90 773.1 0 90 273 60 773.1 0 60 274 52 773.1 0 52 275 53 773.1 0 53 276 142 773.1 0 142 277 322 773.1 0 322 278 413 773.1 0 413 279 421 773.1 0 421 280 307 773.1 0 307 281 823 773.1 0 823 282 925 773.1 0 925 283 1145 773.1 0 1145 284 330 773.1 0 330 285 55 773.1 0 55 286 53 773.1 0 53 287 79 773.1 0 79 288 201 773.1 0 201 289 168 773.1 0 168 290 251 773.1 0 251 291 82 773.1 0 82 292 87 773.1 0 87 293 61 773.1 0 61 294 82 773.1 0 82 295 98 773.1 0 98 296 100 773.1 0 100 297 49 773.1 0 49 298 47 773.1 0 47 299 40 773.1 0 40 300 41 773.1 0 41 301 63 773.1 0 63 Finished in 12.93 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 174,844 Reads with adapters: 31,058 (17.8%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 31,025 (17.7%) Total basepairs processed: 48,887,790 bp Total written (filtered): 8,389,003 bp (17.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31058 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 7 0.0 3 0 0 7 19 81 0.0 3 0 76 3 1 1 20 30364 0.0 4 29910 434 17 1 2 21 61 0.0 4 13 47 0 0 1 22 6 0.0 4 2 0 3 0 1 23 23 0.0 4 3 0 0 18 2 26 1 0.0 4 0 0 0 1 60 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 121 4 0.0 4 0 0 0 0 4 122 400 0.0 4 0 0 0 0 400 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 64 0.0 4 0 0 0 0 64 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 164 1 0.0 4 1 176 1 0.0 4 1 188 1 0.0 4 1 211 1 0.0 4 0 0 0 0 1 223 5 0.0 4 0 0 0 0 5 225 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 253 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 1 1 264 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 1 278 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.76 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 31,025 Reads with adapters: 5,068 (16.3%) Reads written (passing filters): 31,025 (100.0%) Total basepairs processed: 8,389,003 bp Total written (filtered): 8,340,867 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5068 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 1.6% G: 96.4% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 484.8 0 5 4 2 121.2 0 2 5 2 30.3 0 2 6 1 7.6 0 1 9 3375 0.1 0 75 3300 10 1459 0.0 1 52 1407 11 208 0.0 1 1 207 12 7 0.0 1 1 6 19 4 0.0 1 3 1 22 1 0.0 1 1 81 1 0.0 1 1 150 1 0.0 1 0 1 208 1 0.0 1 0 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.