This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.94 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 105,731 (3.3%) Reads that were too short: 15,606 (0.5%) Reads written (passing filters): 90,125 (2.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 24,145,086 bp (2.5%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 105731 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1221 773.1 0 1221 7 32548 773.1 0 32548 8 400 773.1 0 400 9 35414 773.1 0 35414 10 85 773.1 0 85 11 87 773.1 0 87 12 36 773.1 0 36 13 44 773.1 0 44 14 40 773.1 0 40 15 46 773.1 0 46 16 36 773.1 0 36 17 79 773.1 0 79 18 32 773.1 0 32 19 41 773.1 0 41 20 49 773.1 0 49 21 42 773.1 0 42 22 60 773.1 0 60 23 46 773.1 0 46 24 46 773.1 0 46 25 53 773.1 0 53 26 34 773.1 0 34 27 31 773.1 0 31 28 34 773.1 0 34 29 81 773.1 0 81 30 56 773.1 0 56 31 65 773.1 0 65 32 43 773.1 0 43 33 55 773.1 0 55 34 37 773.1 0 37 35 40 773.1 0 40 36 46 773.1 0 46 37 63 773.1 0 63 38 49 773.1 0 49 39 51 773.1 0 51 40 61 773.1 0 61 41 46 773.1 0 46 42 55 773.1 0 55 43 38 773.1 0 38 44 40 773.1 0 40 45 29 773.1 0 29 46 53 773.1 0 53 47 57 773.1 0 57 48 31 773.1 0 31 49 61 773.1 0 61 50 41 773.1 0 41 51 27 773.1 0 27 52 62 773.1 0 62 53 67 773.1 0 67 54 50 773.1 0 50 55 64 773.1 0 64 56 49 773.1 0 49 57 34 773.1 0 34 58 55 773.1 0 55 59 41 773.1 0 41 60 48 773.1 0 48 61 41 773.1 0 41 62 54 773.1 0 54 63 51 773.1 0 51 64 51 773.1 0 51 65 76 773.1 0 76 66 46 773.1 0 46 67 68 773.1 0 68 68 69 773.1 0 69 69 64 773.1 0 64 70 104 773.1 0 104 71 697 773.1 0 697 72 379 773.1 0 379 73 477 773.1 0 477 74 126 773.1 0 126 75 168 773.1 0 168 76 62 773.1 0 62 77 36 773.1 0 36 78 70 773.1 0 70 79 74 773.1 0 74 80 60 773.1 0 60 81 75 773.1 0 75 82 89 773.1 0 89 83 51 773.1 0 51 84 47 773.1 0 47 85 58 773.1 0 58 86 56 773.1 0 56 87 68 773.1 0 68 88 52 773.1 0 52 89 57 773.1 0 57 90 63 773.1 0 63 91 56 773.1 0 56 92 48 773.1 0 48 93 47 773.1 0 47 94 39 773.1 0 39 95 40 773.1 0 40 96 61 773.1 0 61 97 47 773.1 0 47 98 44 773.1 0 44 99 107 773.1 0 107 100 100 773.1 0 100 101 852 773.1 0 852 102 419 773.1 0 419 103 545 773.1 0 545 104 84 773.1 0 84 105 59 773.1 0 59 106 74 773.1 0 74 107 42 773.1 0 42 108 63 773.1 0 63 109 66 773.1 0 66 110 75 773.1 0 75 111 227 773.1 0 227 112 219 773.1 0 219 113 320 773.1 0 320 114 152 773.1 0 152 115 237 773.1 0 237 116 81 773.1 0 81 117 113 773.1 0 113 118 54 773.1 0 54 119 68 773.1 0 68 120 55 773.1 0 55 121 62 773.1 0 62 122 67 773.1 0 67 123 122 773.1 0 122 124 133 773.1 0 133 125 96 773.1 0 96 126 89 773.1 0 89 127 62 773.1 0 62 128 65 773.1 0 65 129 60 773.1 0 60 130 106 773.1 0 106 131 99 773.1 0 99 132 540 773.1 0 540 133 185 773.1 0 185 134 177 773.1 0 177 135 68 773.1 0 68 136 103 773.1 0 103 137 193 773.1 0 193 138 195 773.1 0 195 139 212 773.1 0 212 140 92 773.1 0 92 141 112 773.1 0 112 142 131 773.1 0 131 143 124 773.1 0 124 144 304 773.1 0 304 145 219 773.1 0 219 146 325 773.1 0 325 147 74 773.1 0 74 148 85 773.1 0 85 149 159 773.1 0 159 150 147 773.1 0 147 151 134 773.1 0 134 152 72 773.1 0 72 153 55 773.1 0 55 154 145 773.1 0 145 155 115 773.1 0 115 156 166 773.1 0 166 157 83 773.1 0 83 158 113 773.1 0 113 159 97 773.1 0 97 160 87 773.1 0 87 161 69 773.1 0 69 162 83 773.1 0 83 163 92 773.1 0 92 164 130 773.1 0 130 165 92 773.1 0 92 166 109 773.1 0 109 167 51 773.1 0 51 168 87 773.1 0 87 169 131 773.1 0 131 170 433 773.1 0 433 171 302 773.1 0 302 172 313 773.1 0 313 173 57 773.1 0 57 174 43 773.1 0 43 175 54 773.1 0 54 176 66 773.1 0 66 177 123 773.1 0 123 178 321 773.1 0 321 179 309 773.1 0 309 180 264 773.1 0 264 181 59 773.1 0 59 182 58 773.1 0 58 183 83 773.1 0 83 184 71 773.1 0 71 185 83 773.1 0 83 186 81 773.1 0 81 187 81 773.1 0 81 188 93 773.1 0 93 189 159 773.1 0 159 190 64 773.1 0 64 191 54 773.1 0 54 192 48 773.1 0 48 193 39 773.1 0 39 194 61 773.1 0 61 195 52 773.1 0 52 196 69 773.1 0 69 197 95 773.1 0 95 198 97 773.1 0 97 199 124 773.1 0 124 200 96 773.1 0 96 201 74 773.1 0 74 202 115 773.1 0 115 203 99 773.1 0 99 204 203 773.1 0 203 205 173 773.1 0 173 206 126 773.1 0 126 207 69 773.1 0 69 208 50 773.1 0 50 209 76 773.1 0 76 210 130 773.1 0 130 211 202 773.1 0 202 212 115 773.1 0 115 213 85 773.1 0 85 214 96 773.1 0 96 215 81 773.1 0 81 216 73 773.1 0 73 217 67 773.1 0 67 218 80 773.1 0 80 219 83 773.1 0 83 220 71 773.1 0 71 221 66 773.1 0 66 222 61 773.1 0 61 223 111 773.1 0 111 224 159 773.1 0 159 225 181 773.1 0 181 226 245 773.1 0 245 227 209 773.1 0 209 228 167 773.1 0 167 229 106 773.1 0 106 230 61 773.1 0 61 231 71 773.1 0 71 232 69 773.1 0 69 233 82 773.1 0 82 234 68 773.1 0 68 235 79 773.1 0 79 236 106 773.1 0 106 237 93 773.1 0 93 238 91 773.1 0 91 239 77 773.1 0 77 240 74 773.1 0 74 241 73 773.1 0 73 242 67 773.1 0 67 243 113 773.1 0 113 244 172 773.1 0 172 245 232 773.1 0 232 246 197 773.1 0 197 247 286 773.1 0 286 248 191 773.1 0 191 249 101 773.1 0 101 250 80 773.1 0 80 251 59 773.1 0 59 252 72 773.1 0 72 253 69 773.1 0 69 254 76 773.1 0 76 255 82 773.1 0 82 256 126 773.1 0 126 257 177 773.1 0 177 258 168 773.1 0 168 259 198 773.1 0 198 260 149 773.1 0 149 261 149 773.1 0 149 262 121 773.1 0 121 263 104 773.1 0 104 264 273 773.1 0 273 265 247 773.1 0 247 266 331 773.1 0 331 267 96 773.1 0 96 268 110 773.1 0 110 269 76 773.1 0 76 270 104 773.1 0 104 271 167 773.1 0 167 272 265 773.1 0 265 273 218 773.1 0 218 274 187 773.1 0 187 275 104 773.1 0 104 276 81 773.1 0 81 277 103 773.1 0 103 278 83 773.1 0 83 279 122 773.1 0 122 280 125 773.1 0 125 281 133 773.1 0 133 282 164 773.1 0 164 283 215 773.1 0 215 284 758 773.1 0 758 285 986 773.1 0 986 286 1159 773.1 0 1159 287 694 773.1 0 694 288 145 773.1 0 145 289 92 773.1 0 92 290 70 773.1 0 70 291 133 773.1 0 133 292 162 773.1 0 162 293 206 773.1 0 206 294 83 773.1 0 83 295 85 773.1 0 85 296 76 773.1 0 76 297 73 773.1 0 73 298 94 773.1 0 94 299 105 773.1 0 105 300 104 773.1 0 104 301 145 773.1 0 145 Finished in 6.32 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 90,125 Reads with adapters: 36,148 (40.1%) Reads that were too short: 53 (0.1%) Reads written (passing filters): 36,095 (40.0%) Total basepairs processed: 24,145,086 bp Total written (filtered): 9,913,236 bp (41.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36148 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 16 0.0 3 0 0 6 10 16 191 0.0 3 0 148 33 10 17 35537 0.0 3 33451 1698 303 85 18 76 0.0 3 8 54 6 8 19 60 0.0 3 0 0 31 29 20 116 0.0 3 0 0 1 115 21 3 0.0 3 0 0 0 3 24 2 0.0 3 1 0 0 1 26 1 0.0 3 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 6 0.0 3 0 0 1 5 51 3 0.0 3 0 0 0 3 52 2 0.0 3 0 0 0 2 54 5 0.0 3 0 0 0 5 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 70 25 0.0 3 0 0 2 23 71 1 0.0 3 0 0 0 1 73 3 0.0 3 0 0 0 3 75 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 1 85 1 0.0 3 0 0 1 87 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 1 179 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 15 0.0 3 0 0 1 14 195 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 208 5 0.0 3 0 0 0 5 221 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.09 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 36,095 Reads with adapters: 1,709 (4.7%) Reads written (passing filters): 36,095 (100.0%) Total basepairs processed: 9,913,236 bp Total written (filtered): 9,908,080 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1709 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.0% C: 4.3% G: 64.7% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1691 564.0 0 1691 4 15 141.0 0 15 5 1 35.2 0 1 9 2 0.1 0 0 2