This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 146.45 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 94,643 (3.0%) Reads that were too short: 9,073 (0.3%) Reads written (passing filters): 85,570 (2.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,636,319 bp (2.5%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 94643 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1326 773.1 0 1326 7 38429 773.1 0 38429 8 284 773.1 0 284 9 31721 773.1 0 31721 10 61 773.1 0 61 11 59 773.1 0 59 12 29 773.1 0 29 13 25 773.1 0 25 14 26 773.1 0 26 15 33 773.1 0 33 16 19 773.1 0 19 17 23 773.1 0 23 18 25 773.1 0 25 19 28 773.1 0 28 20 38 773.1 0 38 21 43 773.1 0 43 22 25 773.1 0 25 23 32 773.1 0 32 24 41 773.1 0 41 25 27 773.1 0 27 26 27 773.1 0 27 27 31 773.1 0 31 28 28 773.1 0 28 29 29 773.1 0 29 30 24 773.1 0 24 31 30 773.1 0 30 32 36 773.1 0 36 33 30 773.1 0 30 34 22 773.1 0 22 35 21 773.1 0 21 36 25 773.1 0 25 37 26 773.1 0 26 38 26 773.1 0 26 39 24 773.1 0 24 40 26 773.1 0 26 41 46 773.1 0 46 42 35 773.1 0 35 43 42 773.1 0 42 44 31 773.1 0 31 45 31 773.1 0 31 46 33 773.1 0 33 47 39 773.1 0 39 48 25 773.1 0 25 49 33 773.1 0 33 50 118 773.1 0 118 51 23 773.1 0 23 52 31 773.1 0 31 53 42 773.1 0 42 54 36 773.1 0 36 55 47 773.1 0 47 56 29 773.1 0 29 57 34 773.1 0 34 58 35 773.1 0 35 59 30 773.1 0 30 60 30 773.1 0 30 61 25 773.1 0 25 62 29 773.1 0 29 63 28 773.1 0 28 64 41 773.1 0 41 65 31 773.1 0 31 66 30 773.1 0 30 67 43 773.1 0 43 68 62 773.1 0 62 69 46 773.1 0 46 70 103 773.1 0 103 71 921 773.1 0 921 72 558 773.1 0 558 73 625 773.1 0 625 74 108 773.1 0 108 75 160 773.1 0 160 76 30 773.1 0 30 77 23 773.1 0 23 78 37 773.1 0 37 79 36 773.1 0 36 80 61 773.1 0 61 81 65 773.1 0 65 82 81 773.1 0 81 83 31 773.1 0 31 84 33 773.1 0 33 85 28 773.1 0 28 86 36 773.1 0 36 87 28 773.1 0 28 88 35 773.1 0 35 89 34 773.1 0 34 90 41 773.1 0 41 91 43 773.1 0 43 92 41 773.1 0 41 93 44 773.1 0 44 94 29 773.1 0 29 95 44 773.1 0 44 96 29 773.1 0 29 97 30 773.1 0 30 98 41 773.1 0 41 99 50 773.1 0 50 100 45 773.1 0 45 101 389 773.1 0 389 102 252 773.1 0 252 103 211 773.1 0 211 104 33 773.1 0 33 105 31 773.1 0 31 106 35 773.1 0 35 107 26 773.1 0 26 108 25 773.1 0 25 109 30 773.1 0 30 110 34 773.1 0 34 111 125 773.1 0 125 112 119 773.1 0 119 113 198 773.1 0 198 114 92 773.1 0 92 115 174 773.1 0 174 116 39 773.1 0 39 117 53 773.1 0 53 118 33 773.1 0 33 119 23 773.1 0 23 120 26 773.1 0 26 121 37 773.1 0 37 122 36 773.1 0 36 123 51 773.1 0 51 124 101 773.1 0 101 125 58 773.1 0 58 126 34 773.1 0 34 127 22 773.1 0 22 128 24 773.1 0 24 129 32 773.1 0 32 130 51 773.1 0 51 131 71 773.1 0 71 132 442 773.1 0 442 133 183 773.1 0 183 134 160 773.1 0 160 135 44 773.1 0 44 136 47 773.1 0 47 137 120 773.1 0 120 138 98 773.1 0 98 139 120 773.1 0 120 140 39 773.1 0 39 141 73 773.1 0 73 142 60 773.1 0 60 143 56 773.1 0 56 144 252 773.1 0 252 145 140 773.1 0 140 146 278 773.1 0 278 147 48 773.1 0 48 148 56 773.1 0 56 149 143 773.1 0 143 150 117 773.1 0 117 151 85 773.1 0 85 152 40 773.1 0 40 153 48 773.1 0 48 154 105 773.1 0 105 155 72 773.1 0 72 156 114 773.1 0 114 157 48 773.1 0 48 158 72 773.1 0 72 159 46 773.1 0 46 160 44 773.1 0 44 161 46 773.1 0 46 162 38 773.1 0 38 163 54 773.1 0 54 164 63 773.1 0 63 165 41 773.1 0 41 166 51 773.1 0 51 167 29 773.1 0 29 168 46 773.1 0 46 169 71 773.1 0 71 170 244 773.1 0 244 171 201 773.1 0 201 172 229 773.1 0 229 173 23 773.1 0 23 174 30 773.1 0 30 175 35 773.1 0 35 176 36 773.1 0 36 177 59 773.1 0 59 178 137 773.1 0 137 179 137 773.1 0 137 180 103 773.1 0 103 181 28 773.1 0 28 182 34 773.1 0 34 183 33 773.1 0 33 184 44 773.1 0 44 185 49 773.1 0 49 186 45 773.1 0 45 187 66 773.1 0 66 188 49 773.1 0 49 189 162 773.1 0 162 190 40 773.1 0 40 191 30 773.1 0 30 192 27 773.1 0 27 193 32 773.1 0 32 194 28 773.1 0 28 195 34 773.1 0 34 196 48 773.1 0 48 197 54 773.1 0 54 198 54 773.1 0 54 199 48 773.1 0 48 200 56 773.1 0 56 201 47 773.1 0 47 202 53 773.1 0 53 203 55 773.1 0 55 204 67 773.1 0 67 205 68 773.1 0 68 206 46 773.1 0 46 207 35 773.1 0 35 208 29 773.1 0 29 209 36 773.1 0 36 210 86 773.1 0 86 211 150 773.1 0 150 212 81 773.1 0 81 213 48 773.1 0 48 214 55 773.1 0 55 215 43 773.1 0 43 216 30 773.1 0 30 217 51 773.1 0 51 218 65 773.1 0 65 219 47 773.1 0 47 220 43 773.1 0 43 221 34 773.1 0 34 222 51 773.1 0 51 223 62 773.1 0 62 224 111 773.1 0 111 225 93 773.1 0 93 226 131 773.1 0 131 227 121 773.1 0 121 228 98 773.1 0 98 229 87 773.1 0 87 230 33 773.1 0 33 231 35 773.1 0 35 232 34 773.1 0 34 233 45 773.1 0 45 234 41 773.1 0 41 235 49 773.1 0 49 236 64 773.1 0 64 237 55 773.1 0 55 238 49 773.1 0 49 239 38 773.1 0 38 240 39 773.1 0 39 241 44 773.1 0 44 242 47 773.1 0 47 243 55 773.1 0 55 244 81 773.1 0 81 245 107 773.1 0 107 246 112 773.1 0 112 247 199 773.1 0 199 248 102 773.1 0 102 249 73 773.1 0 73 250 36 773.1 0 36 251 36 773.1 0 36 252 40 773.1 0 40 253 51 773.1 0 51 254 45 773.1 0 45 255 67 773.1 0 67 256 72 773.1 0 72 257 91 773.1 0 91 258 99 773.1 0 99 259 139 773.1 0 139 260 87 773.1 0 87 261 103 773.1 0 103 262 89 773.1 0 89 263 76 773.1 0 76 264 130 773.1 0 130 265 159 773.1 0 159 266 172 773.1 0 172 267 54 773.1 0 54 268 66 773.1 0 66 269 45 773.1 0 45 270 49 773.1 0 49 271 100 773.1 0 100 272 143 773.1 0 143 273 121 773.1 0 121 274 77 773.1 0 77 275 57 773.1 0 57 276 63 773.1 0 63 277 89 773.1 0 89 278 47 773.1 0 47 279 83 773.1 0 83 280 72 773.1 0 72 281 78 773.1 0 78 282 81 773.1 0 81 283 98 773.1 0 98 284 405 773.1 0 405 285 631 773.1 0 631 286 783 773.1 0 783 287 299 773.1 0 299 288 88 773.1 0 88 289 46 773.1 0 46 290 37 773.1 0 37 291 70 773.1 0 70 292 105 773.1 0 105 293 78 773.1 0 78 294 51 773.1 0 51 295 60 773.1 0 60 296 48 773.1 0 48 297 50 773.1 0 50 298 75 773.1 0 75 299 62 773.1 0 62 300 86 773.1 0 86 301 106 773.1 0 106 Finished in 6.54 s (76 us/read; 0.79 M reads/minute). === Summary === Total reads processed: 85,570 Reads with adapters: 39,463 (46.1%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 39,448 (46.1%) Total basepairs processed: 23,636,319 bp Total written (filtered): 10,792,934 bp (45.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 39463 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 115 0.0 3 0 113 2 20 38392 0.0 4 37694 674 19 4 1 21 79 0.0 4 5 73 1 22 8 0.0 4 2 2 2 1 1 23 718 0.0 4 678 14 1 20 5 24 3 0.0 4 0 2 0 1 35 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 126 0.0 4 0 0 0 0 126 128 1 0.0 4 0 0 0 0 1 148 1 0.0 4 1 229 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.13 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 39,448 Reads with adapters: 957 (2.4%) Reads written (passing filters): 39,448 (100.0%) Total basepairs processed: 10,792,934 bp Total written (filtered): 10,782,507 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 957 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.9% C: 4.1% G: 88.9% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 54 616.4 0 54 4 6 154.1 0 6 9 90 0.2 0 5 85 10 43 0.0 1 2 41 11 716 0.0 1 3 713 12 23 0.0 1 0 23 13 11 0.0 1 1 10 15 1 0.0 1 0 1 19 10 0.0 1 9 1 81 1 0.0 1 1 210 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.