This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.72 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 216,444 (6.8%) Reads that were too short: 131,777 (4.2%) Reads written (passing filters): 84,667 (2.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 22,231,784 bp (2.3%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 216444 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 489 773.1 0 489 7 1010 773.1 0 1010 8 31257 773.1 0 31257 9 28942 773.1 0 28942 10 46 773.1 0 46 11 44 773.1 0 44 12 28 773.1 0 28 13 22 773.1 0 22 14 21 773.1 0 21 15 33 773.1 0 33 16 10 773.1 0 10 17 15 773.1 0 15 18 11 773.1 0 11 19 15 773.1 0 15 20 16 773.1 0 16 21 17 773.1 0 17 22 19 773.1 0 19 23 23 773.1 0 23 24 19 773.1 0 19 25 17 773.1 0 17 26 34 773.1 0 34 27 25 773.1 0 25 28 32 773.1 0 32 29 18 773.1 0 18 30 19 773.1 0 19 31 13 773.1 0 13 32 17 773.1 0 17 33 30 773.1 0 30 34 24 773.1 0 24 35 19 773.1 0 19 36 26 773.1 0 26 37 26 773.1 0 26 38 26 773.1 0 26 39 23 773.1 0 23 40 16 773.1 0 16 41 19 773.1 0 19 42 24 773.1 0 24 43 37 773.1 0 37 44 36 773.1 0 36 45 45 773.1 0 45 46 31 773.1 0 31 47 18 773.1 0 18 48 31 773.1 0 31 49 38 773.1 0 38 50 38 773.1 0 38 51 36 773.1 0 36 52 20 773.1 0 20 53 20 773.1 0 20 54 33 773.1 0 33 55 31 773.1 0 31 56 139 773.1 0 139 57 153 773.1 0 153 58 107 773.1 0 107 59 27 773.1 0 27 60 30 773.1 0 30 61 62 773.1 0 62 62 38 773.1 0 38 63 37 773.1 0 37 64 47 773.1 0 47 65 33 773.1 0 33 66 15 773.1 0 15 67 40 773.1 0 40 68 51 773.1 0 51 69 143 773.1 0 143 70 168 773.1 0 168 71 327 773.1 0 327 72 51 773.1 0 51 73 49 773.1 0 49 74 49 773.1 0 49 75 52 773.1 0 52 76 68 773.1 0 68 77 43 773.1 0 43 78 61 773.1 0 61 79 54 773.1 0 54 80 52 773.1 0 52 81 45 773.1 0 45 82 48 773.1 0 48 83 42 773.1 0 42 84 42 773.1 0 42 85 51 773.1 0 51 86 56 773.1 0 56 87 103 773.1 0 103 88 111 773.1 0 111 89 2398 773.1 0 2398 90 2049 773.1 0 2049 91 2655 773.1 0 2655 92 46 773.1 0 46 93 39 773.1 0 39 94 36 773.1 0 36 95 34 773.1 0 34 96 37 773.1 0 37 97 174 773.1 0 174 98 126 773.1 0 126 99 95 773.1 0 95 100 82 773.1 0 82 101 36 773.1 0 36 102 51 773.1 0 51 103 35 773.1 0 35 104 41 773.1 0 41 105 34 773.1 0 34 106 36 773.1 0 36 107 33 773.1 0 33 108 53 773.1 0 53 109 27 773.1 0 27 110 146 773.1 0 146 111 77 773.1 0 77 112 100 773.1 0 100 113 27 773.1 0 27 114 19 773.1 0 19 115 12 773.1 0 12 116 26 773.1 0 26 117 31 773.1 0 31 118 74 773.1 0 74 119 28 773.1 0 28 120 26 773.1 0 26 121 33 773.1 0 33 122 31 773.1 0 31 123 23 773.1 0 23 124 19 773.1 0 19 125 24 773.1 0 24 126 23 773.1 0 23 127 54 773.1 0 54 128 75 773.1 0 75 129 29 773.1 0 29 130 28 773.1 0 28 131 31 773.1 0 31 132 63 773.1 0 63 133 50 773.1 0 50 134 116 773.1 0 116 135 72 773.1 0 72 136 88 773.1 0 88 137 1326 773.1 0 1326 138 543 773.1 0 543 139 991 773.1 0 991 140 33 773.1 0 33 141 25 773.1 0 25 142 34 773.1 0 34 143 26 773.1 0 26 144 33 773.1 0 33 145 31 773.1 0 31 146 75 773.1 0 75 147 43 773.1 0 43 148 122 773.1 0 122 149 149 773.1 0 149 150 183 773.1 0 183 151 33 773.1 0 33 152 34 773.1 0 34 153 31 773.1 0 31 154 32 773.1 0 32 155 48 773.1 0 48 156 49 773.1 0 49 157 63 773.1 0 63 158 147 773.1 0 147 159 65 773.1 0 65 160 64 773.1 0 64 161 67 773.1 0 67 162 72 773.1 0 72 163 23 773.1 0 23 164 36 773.1 0 36 165 43 773.1 0 43 166 40 773.1 0 40 167 35 773.1 0 35 168 383 773.1 0 383 169 256 773.1 0 256 170 136 773.1 0 136 171 20 773.1 0 20 172 25 773.1 0 25 173 32 773.1 0 32 174 78 773.1 0 78 175 116 773.1 0 116 176 1389 773.1 0 1389 177 951 773.1 0 951 178 1124 773.1 0 1124 179 76 773.1 0 76 180 43 773.1 0 43 181 17 773.1 0 17 182 72 773.1 0 72 183 58 773.1 0 58 184 45 773.1 0 45 185 39 773.1 0 39 186 37 773.1 0 37 187 39 773.1 0 39 188 31 773.1 0 31 189 69 773.1 0 69 190 36 773.1 0 36 191 35 773.1 0 35 192 32 773.1 0 32 193 31 773.1 0 31 194 41 773.1 0 41 195 37 773.1 0 37 196 53 773.1 0 53 197 43 773.1 0 43 198 35 773.1 0 35 199 22 773.1 0 22 200 28 773.1 0 28 201 47 773.1 0 47 202 122 773.1 0 122 203 114 773.1 0 114 204 95 773.1 0 95 205 72 773.1 0 72 206 65 773.1 0 65 207 106 773.1 0 106 208 140 773.1 0 140 209 139 773.1 0 139 210 169 773.1 0 169 211 110 773.1 0 110 212 134 773.1 0 134 213 70 773.1 0 70 214 43 773.1 0 43 215 75 773.1 0 75 216 77 773.1 0 77 217 93 773.1 0 93 218 115 773.1 0 115 219 353 773.1 0 353 220 2177 773.1 0 2177 221 1450 773.1 0 1450 222 2353 773.1 0 2353 223 289 773.1 0 289 224 49 773.1 0 49 225 98 773.1 0 98 226 48 773.1 0 48 227 56 773.1 0 56 228 29 773.1 0 29 229 34 773.1 0 34 230 100 773.1 0 100 231 130 773.1 0 130 232 171 773.1 0 171 233 112 773.1 0 112 234 71 773.1 0 71 235 72 773.1 0 72 236 58 773.1 0 58 237 46 773.1 0 46 238 39 773.1 0 39 239 118 773.1 0 118 240 157 773.1 0 157 241 165 773.1 0 165 242 142 773.1 0 142 243 116 773.1 0 116 244 164 773.1 0 164 245 132 773.1 0 132 246 66 773.1 0 66 247 66 773.1 0 66 248 51 773.1 0 51 249 102 773.1 0 102 250 110 773.1 0 110 251 122 773.1 0 122 252 52 773.1 0 52 253 101 773.1 0 101 254 94 773.1 0 94 255 202 773.1 0 202 256 135 773.1 0 135 257 138 773.1 0 138 258 136 773.1 0 136 259 88 773.1 0 88 260 100 773.1 0 100 261 128 773.1 0 128 262 285 773.1 0 285 263 391 773.1 0 391 264 411 773.1 0 411 265 322 773.1 0 322 266 192 773.1 0 192 267 148 773.1 0 148 268 407 773.1 0 407 269 978 773.1 0 978 270 1831 773.1 0 1831 271 986 773.1 0 986 272 460 773.1 0 460 273 192 773.1 0 192 274 3463 773.1 0 3463 275 3306 773.1 0 3306 276 1705 773.1 0 1705 277 168 773.1 0 168 278 276 773.1 0 276 279 362 773.1 0 362 280 586 773.1 0 586 281 1292 773.1 0 1292 282 7351 773.1 0 7351 283 33134 773.1 0 33134 284 28227 773.1 0 28227 285 25244 773.1 0 25244 286 4847 773.1 0 4847 287 120 773.1 0 120 288 628 773.1 0 628 289 565 773.1 0 565 290 867 773.1 0 867 291 325 773.1 0 325 292 50 773.1 0 50 293 36 773.1 0 36 294 38 773.1 0 38 295 48 773.1 0 48 296 69 773.1 0 69 297 91 773.1 0 91 298 78 773.1 0 78 299 61 773.1 0 61 300 41 773.1 0 41 301 37 773.1 0 37 Finished in 5.78 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 84,667 Reads with adapters: 32,042 (37.8%) Reads that were too short: 53 (0.1%) Reads written (passing filters): 31,989 (37.8%) Total basepairs processed: 22,231,784 bp Total written (filtered): 8,797,787 bp (39.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32042 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 32 0.0 3 0 0 23 9 16 301 0.0 3 0 265 22 14 17 31251 0.0 3 29639 1351 220 41 18 71 0.0 3 4 54 7 6 19 86 0.0 3 1 0 52 33 20 47 0.0 3 0 0 0 47 25 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 1 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 43 13 0.0 3 0 0 1 12 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 1 1 55 1 0.0 3 0 0 0 1 56 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 82 6 0.0 3 0 0 0 6 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 100 3 0.0 3 0 0 0 3 101 65 0.0 3 0 0 0 65 102 56 0.0 3 0 0 1 55 103 2 0.0 3 0 0 0 2 105 9 0.0 3 0 0 0 9 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 124 4 0.0 3 0 0 0 4 125 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 5 0.0 3 0 0 0 5 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 203 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.75 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 31,989 Reads with adapters: 219 (0.7%) Reads written (passing filters): 31,989 (100.0%) Total basepairs processed: 8,797,787 bp Total written (filtered): 8,796,990 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 219 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.5% C: 9.1% G: 26.5% T: 6.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 164 499.8 0 164 4 38 125.0 0 38 5 5 31.2 0 5 7 1 2.0 0 1 11 11 0.0 1 0 11