This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.70 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 188,364 (5.9%) Reads that were too short: 105,563 (3.3%) Reads written (passing filters): 82,801 (2.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,971,562 bp (2.3%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 188364 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 450 773.1 0 450 7 802 773.1 0 802 8 29652 773.1 0 29652 9 32602 773.1 0 32602 10 47 773.1 0 47 11 21 773.1 0 21 12 5 773.1 0 5 13 4 773.1 0 4 14 7 773.1 0 7 15 9 773.1 0 9 16 4 773.1 0 4 17 5 773.1 0 5 18 6 773.1 0 6 19 7 773.1 0 7 20 7 773.1 0 7 21 53 773.1 0 53 22 14 773.1 0 14 23 8 773.1 0 8 24 9 773.1 0 9 25 11 773.1 0 11 26 7 773.1 0 7 27 7 773.1 0 7 28 19 773.1 0 19 29 7 773.1 0 7 30 6 773.1 0 6 31 4 773.1 0 4 32 12 773.1 0 12 33 8 773.1 0 8 34 9 773.1 0 9 35 3 773.1 0 3 36 12 773.1 0 12 37 13 773.1 0 13 38 23 773.1 0 23 39 8 773.1 0 8 40 2 773.1 0 2 41 9 773.1 0 9 42 11 773.1 0 11 43 23 773.1 0 23 44 40 773.1 0 40 45 33 773.1 0 33 46 19 773.1 0 19 47 9 773.1 0 9 48 15 773.1 0 15 49 27 773.1 0 27 50 23 773.1 0 23 51 25 773.1 0 25 52 14 773.1 0 14 53 11 773.1 0 11 54 5 773.1 0 5 55 17 773.1 0 17 56 527 773.1 0 527 57 135 773.1 0 135 58 44 773.1 0 44 59 15 773.1 0 15 60 16 773.1 0 16 61 31 773.1 0 31 62 33 773.1 0 33 63 17 773.1 0 17 64 21 773.1 0 21 65 25 773.1 0 25 66 11 773.1 0 11 67 21 773.1 0 21 68 27 773.1 0 27 69 99 773.1 0 99 70 76 773.1 0 76 71 134 773.1 0 134 72 25 773.1 0 25 73 26 773.1 0 26 74 25 773.1 0 25 75 37 773.1 0 37 76 52 773.1 0 52 77 25 773.1 0 25 78 27 773.1 0 27 79 35 773.1 0 35 80 36 773.1 0 36 81 32 773.1 0 32 82 27 773.1 0 27 83 23 773.1 0 23 84 39 773.1 0 39 85 43 773.1 0 43 86 41 773.1 0 41 87 62 773.1 0 62 88 81 773.1 0 81 89 2162 773.1 0 2162 90 1542 773.1 0 1542 91 2025 773.1 0 2025 92 21 773.1 0 21 93 13 773.1 0 13 94 9 773.1 0 9 95 14 773.1 0 14 96 13 773.1 0 13 97 45 773.1 0 45 98 42 773.1 0 42 99 28 773.1 0 28 100 44 773.1 0 44 101 25 773.1 0 25 102 24 773.1 0 24 103 17 773.1 0 17 104 16 773.1 0 16 105 22 773.1 0 22 106 18 773.1 0 18 107 22 773.1 0 22 108 15 773.1 0 15 109 18 773.1 0 18 110 99 773.1 0 99 111 34 773.1 0 34 112 74 773.1 0 74 113 19 773.1 0 19 114 16 773.1 0 16 115 5 773.1 0 5 116 13 773.1 0 13 117 16 773.1 0 16 118 53 773.1 0 53 119 12 773.1 0 12 120 25 773.1 0 25 121 9 773.1 0 9 122 18 773.1 0 18 123 8 773.1 0 8 124 15 773.1 0 15 125 19 773.1 0 19 126 14 773.1 0 14 127 25 773.1 0 25 128 31 773.1 0 31 129 13 773.1 0 13 130 18 773.1 0 18 131 10 773.1 0 10 132 40 773.1 0 40 133 26 773.1 0 26 134 74 773.1 0 74 135 46 773.1 0 46 136 76 773.1 0 76 137 1318 773.1 0 1318 138 548 773.1 0 548 139 1001 773.1 0 1001 140 18 773.1 0 18 141 20 773.1 0 20 142 16 773.1 0 16 143 10 773.1 0 10 144 13 773.1 0 13 145 21 773.1 0 21 146 18 773.1 0 18 147 23 773.1 0 23 148 64 773.1 0 64 149 52 773.1 0 52 150 69 773.1 0 69 151 16 773.1 0 16 152 16 773.1 0 16 153 19 773.1 0 19 154 13 773.1 0 13 155 28 773.1 0 28 156 25 773.1 0 25 157 34 773.1 0 34 158 100 773.1 0 100 159 25 773.1 0 25 160 37 773.1 0 37 161 29 773.1 0 29 162 29 773.1 0 29 163 28 773.1 0 28 164 20 773.1 0 20 165 18 773.1 0 18 166 32 773.1 0 32 167 24 773.1 0 24 168 530 773.1 0 530 169 329 773.1 0 329 170 163 773.1 0 163 171 19 773.1 0 19 172 31 773.1 0 31 173 40 773.1 0 40 174 70 773.1 0 70 175 101 773.1 0 101 176 1894 773.1 0 1894 177 1044 773.1 0 1044 178 1456 773.1 0 1456 179 38 773.1 0 38 180 19 773.1 0 19 181 16 773.1 0 16 182 24 773.1 0 24 183 22 773.1 0 22 184 23 773.1 0 23 185 10 773.1 0 10 186 15 773.1 0 15 187 24 773.1 0 24 188 11 773.1 0 11 189 40 773.1 0 40 190 29 773.1 0 29 191 16 773.1 0 16 192 23 773.1 0 23 193 17 773.1 0 17 194 29 773.1 0 29 195 17 773.1 0 17 196 26 773.1 0 26 197 13 773.1 0 13 198 20 773.1 0 20 199 14 773.1 0 14 200 17 773.1 0 17 201 22 773.1 0 22 202 48 773.1 0 48 203 56 773.1 0 56 204 40 773.1 0 40 205 47 773.1 0 47 206 38 773.1 0 38 207 61 773.1 0 61 208 92 773.1 0 92 209 94 773.1 0 94 210 77 773.1 0 77 211 43 773.1 0 43 212 59 773.1 0 59 213 29 773.1 0 29 214 41 773.1 0 41 215 41 773.1 0 41 216 65 773.1 0 65 217 84 773.1 0 84 218 87 773.1 0 87 219 269 773.1 0 269 220 2524 773.1 0 2524 221 1388 773.1 0 1388 222 2464 773.1 0 2464 223 225 773.1 0 225 224 31 773.1 0 31 225 60 773.1 0 60 226 28 773.1 0 28 227 34 773.1 0 34 228 26 773.1 0 26 229 20 773.1 0 20 230 59 773.1 0 59 231 63 773.1 0 63 232 80 773.1 0 80 233 80 773.1 0 80 234 42 773.1 0 42 235 33 773.1 0 33 236 26 773.1 0 26 237 38 773.1 0 38 238 22 773.1 0 22 239 81 773.1 0 81 240 75 773.1 0 75 241 71 773.1 0 71 242 89 773.1 0 89 243 56 773.1 0 56 244 94 773.1 0 94 245 103 773.1 0 103 246 61 773.1 0 61 247 58 773.1 0 58 248 34 773.1 0 34 249 46 773.1 0 46 250 68 773.1 0 68 251 78 773.1 0 78 252 37 773.1 0 37 253 73 773.1 0 73 254 81 773.1 0 81 255 131 773.1 0 131 256 78 773.1 0 78 257 98 773.1 0 98 258 86 773.1 0 86 259 80 773.1 0 80 260 67 773.1 0 67 261 85 773.1 0 85 262 141 773.1 0 141 263 156 773.1 0 156 264 156 773.1 0 156 265 159 773.1 0 159 266 149 773.1 0 149 267 118 773.1 0 118 268 222 773.1 0 222 269 512 773.1 0 512 270 796 773.1 0 796 271 548 773.1 0 548 272 250 773.1 0 250 273 189 773.1 0 189 274 4574 773.1 0 4574 275 4557 773.1 0 4557 276 2259 773.1 0 2259 277 118 773.1 0 118 278 191 773.1 0 191 279 252 773.1 0 252 280 408 773.1 0 408 281 1128 773.1 0 1128 282 4979 773.1 0 4979 283 29883 773.1 0 29883 284 19208 773.1 0 19208 285 19687 773.1 0 19687 286 3085 773.1 0 3085 287 77 773.1 0 77 288 371 773.1 0 371 289 252 773.1 0 252 290 362 773.1 0 362 291 173 773.1 0 173 292 26 773.1 0 26 293 29 773.1 0 29 294 29 773.1 0 29 295 37 773.1 0 37 296 42 773.1 0 42 297 55 773.1 0 55 298 47 773.1 0 47 299 43 773.1 0 43 300 34 773.1 0 34 301 17 773.1 0 17 Finished in 6.02 s (73 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 82,801 Reads with adapters: 33,515 (40.5%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 33,491 (40.4%) Total basepairs processed: 21,971,562 bp Total written (filtered): 9,106,873 bp (41.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33515 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 5 19 85 0.0 3 0 82 1 1 1 20 33261 0.0 4 32756 478 25 1 1 21 65 0.0 4 11 52 2 22 9 0.0 4 0 0 8 0 1 23 30 0.0 4 0 0 0 28 2 24 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 23 0.0 4 0 0 0 0 23 131 6 0.0 4 0 0 0 0 6 138 1 0.0 4 0 0 0 0 1 146 4 0.0 4 0 0 0 0 4 147 2 0.0 4 0 0 0 2 153 4 0.0 4 0 0 0 0 4 206 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.90 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 33,491 Reads with adapters: 5,932 (17.7%) Reads written (passing filters): 33,491 (100.0%) Total basepairs processed: 9,106,873 bp Total written (filtered): 9,048,417 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5932 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.5% G: 96.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 523.3 0 5 4 4 130.8 0 4 5 2 32.7 0 2 6 12 8.2 0 12 7 2 2.0 0 2 8 1 0.5 0 1 9 4123 0.1 0 85 4038 10 1607 0.0 1 56 1551 11 153 0.0 1 2 151 12 5 0.0 1 0 5 13 1 0.0 1 0 1 150 4 0.0 1 0 4 189 1 0.0 1 0 1 190 1 0.0 1 0 1 208 2 0.0 1 1 1 209 1 0.0 1 0 1 223 8 0.0 1 7 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.