This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 151.18 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 273,363 (8.6%) Reads that were too short: 27,198 (0.9%) Reads written (passing filters): 246,165 (7.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 61,239,355 bp (6.4%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 273363 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2251 773.1 0 2251 7 78040 773.1 0 78040 8 63307 773.1 0 63307 9 104 773.1 0 104 10 56 773.1 0 56 11 74 773.1 0 74 12 53 773.1 0 53 13 61 773.1 0 61 14 61 773.1 0 61 15 46 773.1 0 46 16 53 773.1 0 53 17 55 773.1 0 55 18 103 773.1 0 103 19 88 773.1 0 88 20 107 773.1 0 107 21 59 773.1 0 59 22 58 773.1 0 58 23 70 773.1 0 70 24 49 773.1 0 49 25 42 773.1 0 42 26 47 773.1 0 47 27 48 773.1 0 48 28 54 773.1 0 54 29 165 773.1 0 165 30 47 773.1 0 47 31 44 773.1 0 44 32 53 773.1 0 53 33 68 773.1 0 68 34 69 773.1 0 69 35 115 773.1 0 115 36 63 773.1 0 63 37 62 773.1 0 62 38 62 773.1 0 62 39 54 773.1 0 54 40 59 773.1 0 59 41 58 773.1 0 58 42 58 773.1 0 58 43 50 773.1 0 50 44 49 773.1 0 49 45 65 773.1 0 65 46 72 773.1 0 72 47 65 773.1 0 65 48 71 773.1 0 71 49 66 773.1 0 66 50 85 773.1 0 85 51 54 773.1 0 54 52 78 773.1 0 78 53 74 773.1 0 74 54 64 773.1 0 64 55 71 773.1 0 71 56 96 773.1 0 96 57 49 773.1 0 49 58 77 773.1 0 77 59 60 773.1 0 60 60 75 773.1 0 75 61 77 773.1 0 77 62 80 773.1 0 80 63 78 773.1 0 78 64 110 773.1 0 110 65 136 773.1 0 136 66 229 773.1 0 229 67 413 773.1 0 413 68 187 773.1 0 187 69 190 773.1 0 190 70 529 773.1 0 529 71 319 773.1 0 319 72 265 773.1 0 265 73 208 773.1 0 208 74 174 773.1 0 174 75 234 773.1 0 234 76 258 773.1 0 258 77 398 773.1 0 398 78 589 773.1 0 589 79 1092 773.1 0 1092 80 18526 773.1 0 18526 81 17096 773.1 0 17096 82 18979 773.1 0 18979 83 181 773.1 0 181 84 150 773.1 0 150 85 91 773.1 0 91 86 136 773.1 0 136 87 101 773.1 0 101 88 242 773.1 0 242 89 96 773.1 0 96 90 46 773.1 0 46 91 47 773.1 0 47 92 46 773.1 0 46 93 84 773.1 0 84 94 58 773.1 0 58 95 88 773.1 0 88 96 105 773.1 0 105 97 81 773.1 0 81 98 34 773.1 0 34 99 60 773.1 0 60 100 38 773.1 0 38 101 42 773.1 0 42 102 50 773.1 0 50 103 50 773.1 0 50 104 35 773.1 0 35 105 41 773.1 0 41 106 48 773.1 0 48 107 48 773.1 0 48 108 43 773.1 0 43 109 46 773.1 0 46 110 57 773.1 0 57 111 54 773.1 0 54 112 52 773.1 0 52 113 63 773.1 0 63 114 52 773.1 0 52 115 34 773.1 0 34 116 41 773.1 0 41 117 82 773.1 0 82 118 97 773.1 0 97 119 165 773.1 0 165 120 202 773.1 0 202 121 246 773.1 0 246 122 250 773.1 0 250 123 68 773.1 0 68 124 110 773.1 0 110 125 109 773.1 0 109 126 112 773.1 0 112 127 203 773.1 0 203 128 184 773.1 0 184 129 287 773.1 0 287 130 56 773.1 0 56 131 57 773.1 0 57 132 70 773.1 0 70 133 64 773.1 0 64 134 43 773.1 0 43 135 75 773.1 0 75 136 51 773.1 0 51 137 62 773.1 0 62 138 64 773.1 0 64 139 100 773.1 0 100 140 99 773.1 0 99 141 107 773.1 0 107 142 96 773.1 0 96 143 132 773.1 0 132 144 170 773.1 0 170 145 102 773.1 0 102 146 384 773.1 0 384 147 107 773.1 0 107 148 111 773.1 0 111 149 139 773.1 0 139 150 124 773.1 0 124 151 64 773.1 0 64 152 63 773.1 0 63 153 59 773.1 0 59 154 71 773.1 0 71 155 70 773.1 0 70 156 39 773.1 0 39 157 43 773.1 0 43 158 52 773.1 0 52 159 52 773.1 0 52 160 53 773.1 0 53 161 54 773.1 0 54 162 65 773.1 0 65 163 48 773.1 0 48 164 77 773.1 0 77 165 51 773.1 0 51 166 76 773.1 0 76 167 84 773.1 0 84 168 335 773.1 0 335 169 121 773.1 0 121 170 69 773.1 0 69 171 87 773.1 0 87 172 82 773.1 0 82 173 102 773.1 0 102 174 174 773.1 0 174 175 303 773.1 0 303 176 112 773.1 0 112 177 143 773.1 0 143 178 156 773.1 0 156 179 91 773.1 0 91 180 108 773.1 0 108 181 154 773.1 0 154 182 160 773.1 0 160 183 173 773.1 0 173 184 131 773.1 0 131 185 288 773.1 0 288 186 303 773.1 0 303 187 395 773.1 0 395 188 4047 773.1 0 4047 189 10143 773.1 0 10143 190 10252 773.1 0 10252 191 798 773.1 0 798 192 600 773.1 0 600 193 578 773.1 0 578 194 283 773.1 0 283 195 114 773.1 0 114 196 156 773.1 0 156 197 70 773.1 0 70 198 68 773.1 0 68 199 78 773.1 0 78 200 368 773.1 0 368 201 83 773.1 0 83 202 77 773.1 0 77 203 81 773.1 0 81 204 115 773.1 0 115 205 138 773.1 0 138 206 1303 773.1 0 1303 207 1199 773.1 0 1199 208 1830 773.1 0 1830 209 273 773.1 0 273 210 96 773.1 0 96 211 427 773.1 0 427 212 585 773.1 0 585 213 794 773.1 0 794 214 201 773.1 0 201 215 50 773.1 0 50 216 50 773.1 0 50 217 55 773.1 0 55 218 60 773.1 0 60 219 51 773.1 0 51 220 47 773.1 0 47 221 63 773.1 0 63 222 76 773.1 0 76 223 76 773.1 0 76 224 53 773.1 0 53 225 77 773.1 0 77 226 56 773.1 0 56 227 87 773.1 0 87 228 100 773.1 0 100 229 132 773.1 0 132 230 129 773.1 0 129 231 127 773.1 0 127 232 68 773.1 0 68 233 86 773.1 0 86 234 82 773.1 0 82 235 106 773.1 0 106 236 65 773.1 0 65 237 91 773.1 0 91 238 70 773.1 0 70 239 87 773.1 0 87 240 94 773.1 0 94 241 114 773.1 0 114 242 83 773.1 0 83 243 72 773.1 0 72 244 79 773.1 0 79 245 77 773.1 0 77 246 83 773.1 0 83 247 107 773.1 0 107 248 145 773.1 0 145 249 83 773.1 0 83 250 92 773.1 0 92 251 103 773.1 0 103 252 127 773.1 0 127 253 101 773.1 0 101 254 82 773.1 0 82 255 150 773.1 0 150 256 175 773.1 0 175 257 198 773.1 0 198 258 150 773.1 0 150 259 95 773.1 0 95 260 100 773.1 0 100 261 94 773.1 0 94 262 107 773.1 0 107 263 81 773.1 0 81 264 70 773.1 0 70 265 80 773.1 0 80 266 111 773.1 0 111 267 124 773.1 0 124 268 287 773.1 0 287 269 437 773.1 0 437 270 362 773.1 0 362 271 2083 773.1 0 2083 272 2310 773.1 0 2310 273 2506 773.1 0 2506 274 553 773.1 0 553 275 113 773.1 0 113 276 101 773.1 0 101 277 92 773.1 0 92 278 123 773.1 0 123 279 125 773.1 0 125 280 156 773.1 0 156 281 82 773.1 0 82 282 832 773.1 0 832 283 809 773.1 0 809 284 1178 773.1 0 1178 285 72 773.1 0 72 286 60 773.1 0 60 287 103 773.1 0 103 288 132 773.1 0 132 289 98 773.1 0 98 290 99 773.1 0 99 291 155 773.1 0 155 292 307 773.1 0 307 293 345 773.1 0 345 294 325 773.1 0 325 295 186 773.1 0 186 296 108 773.1 0 108 297 112 773.1 0 112 298 98 773.1 0 98 299 117 773.1 0 117 300 238 773.1 0 238 301 454 773.1 0 454 Finished in 16.64 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 246,165 Reads with adapters: 78,172 (31.8%) Reads that were too short: 203 (0.1%) Reads written (passing filters): 77,969 (31.7%) Total basepairs processed: 61,239,355 bp Total written (filtered): 21,540,438 bp (35.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 78172 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 28 0.0 3 0 0 17 11 16 532 0.0 3 0 449 57 26 17 76015 0.0 3 71899 3433 537 146 18 152 0.0 3 12 121 10 9 19 151 0.0 3 5 8 87 51 20 705 0.0 3 576 28 5 96 21 1 0.0 3 0 0 1 22 7 0.0 3 0 0 4 3 23 12 0.0 3 0 0 0 12 24 4 0.0 3 1 0 1 2 26 1 0.0 3 1 27 2 0.0 3 0 0 0 2 29 2 0.0 3 2 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 35 4 0.0 3 0 0 1 3 36 1 0.0 3 1 40 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 47 4 0.0 3 0 0 0 4 50 4 0.0 3 0 0 0 4 52 5 0.0 3 3 1 1 53 5 0.0 3 0 0 0 5 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 0 3 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 3 0.0 3 0 0 0 3 81 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 0 2 83 5 0.0 3 0 0 0 5 84 1 0.0 3 0 0 0 1 86 49 0.0 3 0 0 0 49 87 19 0.0 3 1 0 1 17 89 21 0.0 3 0 0 1 20 90 2 0.0 3 0 0 0 2 91 17 0.0 3 0 0 0 17 92 14 0.0 3 0 0 0 14 93 2 0.0 3 1 0 0 1 94 9 0.0 3 0 0 1 8 95 5 0.0 3 0 0 1 4 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 18 0.0 3 0 0 0 18 108 1 0.0 3 0 0 0 1 109 11 0.0 3 0 0 1 10 110 6 0.0 3 0 0 0 6 111 93 0.0 3 0 0 0 93 112 7 0.0 3 0 0 0 7 113 1 0.0 3 0 0 0 1 114 8 0.0 3 0 0 0 8 115 4 0.0 3 0 0 0 4 116 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 19 0.0 3 0 0 0 19 130 6 0.0 3 0 0 0 6 131 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 7 0.0 3 0 0 0 7 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 6 0.0 3 0 0 0 6 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 1 2 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 5 0.0 3 0 0 0 5 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 3 0.0 3 0 0 0 3 189 3 0.0 3 0 0 0 3 190 10 0.0 3 0 0 0 10 191 3 0.0 3 0 0 0 3 193 2 0.0 3 0 0 0 2 194 2 0.0 3 0 1 0 1 195 1 0.0 3 1 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 204 2 0.0 3 0 0 0 2 205 6 0.0 3 0 0 0 6 206 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 212 4 0.0 3 0 0 0 4 213 8 0.0 3 0 0 0 8 215 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 0 4 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 10 0.0 3 0 0 1 9 282 10 0.0 3 0 0 0 10 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.49 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 77,969 Reads with adapters: 682 (0.9%) Reads written (passing filters): 77,969 (100.0%) Total basepairs processed: 21,540,438 bp Total written (filtered): 21,538,351 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 682 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.7% C: 62.3% G: 18.2% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 658 1218.3 0 658 4 17 304.6 0 17 5 3 76.1 0 3 6 3 19.0 0 3 12 1 0.0 1 0 1