This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 151.50 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 258,698 (8.2%) Reads that were too short: 19,856 (0.6%) Reads written (passing filters): 238,842 (7.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 60,394,652 bp (6.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 258698 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1390 773.1 0 1390 7 67815 773.1 0 67815 8 78281 773.1 0 78281 9 79 773.1 0 79 10 48 773.1 0 48 11 35 773.1 0 35 12 40 773.1 0 40 13 29 773.1 0 29 14 33 773.1 0 33 15 25 773.1 0 25 16 31 773.1 0 31 17 32 773.1 0 32 18 44 773.1 0 44 19 53 773.1 0 53 20 42 773.1 0 42 21 42 773.1 0 42 22 49 773.1 0 49 23 48 773.1 0 48 24 46 773.1 0 46 25 38 773.1 0 38 26 33 773.1 0 33 27 50 773.1 0 50 28 48 773.1 0 48 29 140 773.1 0 140 30 56 773.1 0 56 31 48 773.1 0 48 32 51 773.1 0 51 33 49 773.1 0 49 34 41 773.1 0 41 35 108 773.1 0 108 36 45 773.1 0 45 37 49 773.1 0 49 38 50 773.1 0 50 39 47 773.1 0 47 40 59 773.1 0 59 41 35 773.1 0 35 42 65 773.1 0 65 43 44 773.1 0 44 44 27 773.1 0 27 45 54 773.1 0 54 46 52 773.1 0 52 47 45 773.1 0 45 48 146 773.1 0 146 49 52 773.1 0 52 50 118 773.1 0 118 51 52 773.1 0 52 52 48 773.1 0 48 53 48 773.1 0 48 54 43 773.1 0 43 55 49 773.1 0 49 56 62 773.1 0 62 57 38 773.1 0 38 58 42 773.1 0 42 59 40 773.1 0 40 60 64 773.1 0 64 61 71 773.1 0 71 62 71 773.1 0 71 63 54 773.1 0 54 64 86 773.1 0 86 65 142 773.1 0 142 66 177 773.1 0 177 67 390 773.1 0 390 68 184 773.1 0 184 69 167 773.1 0 167 70 585 773.1 0 585 71 358 773.1 0 358 72 259 773.1 0 259 73 222 773.1 0 222 74 156 773.1 0 156 75 211 773.1 0 211 76 218 773.1 0 218 77 310 773.1 0 310 78 439 773.1 0 439 79 845 773.1 0 845 80 17232 773.1 0 17232 81 15933 773.1 0 15933 82 18214 773.1 0 18214 83 101 773.1 0 101 84 98 773.1 0 98 85 49 773.1 0 49 86 38 773.1 0 38 87 51 773.1 0 51 88 228 773.1 0 228 89 47 773.1 0 47 90 30 773.1 0 30 91 26 773.1 0 26 92 48 773.1 0 48 93 43 773.1 0 43 94 25 773.1 0 25 95 48 773.1 0 48 96 46 773.1 0 46 97 40 773.1 0 40 98 33 773.1 0 33 99 39 773.1 0 39 100 38 773.1 0 38 101 28 773.1 0 28 102 29 773.1 0 29 103 56 773.1 0 56 104 33 773.1 0 33 105 29 773.1 0 29 106 34 773.1 0 34 107 32 773.1 0 32 108 35 773.1 0 35 109 40 773.1 0 40 110 29 773.1 0 29 111 48 773.1 0 48 112 37 773.1 0 37 113 32 773.1 0 32 114 26 773.1 0 26 115 18 773.1 0 18 116 26 773.1 0 26 117 31 773.1 0 31 118 47 773.1 0 47 119 81 773.1 0 81 120 97 773.1 0 97 121 149 773.1 0 149 122 142 773.1 0 142 123 41 773.1 0 41 124 61 773.1 0 61 125 68 773.1 0 68 126 65 773.1 0 65 127 206 773.1 0 206 128 138 773.1 0 138 129 210 773.1 0 210 130 47 773.1 0 47 131 44 773.1 0 44 132 31 773.1 0 31 133 51 773.1 0 51 134 48 773.1 0 48 135 62 773.1 0 62 136 23 773.1 0 23 137 32 773.1 0 32 138 49 773.1 0 49 139 83 773.1 0 83 140 48 773.1 0 48 141 55 773.1 0 55 142 72 773.1 0 72 143 70 773.1 0 70 144 142 773.1 0 142 145 74 773.1 0 74 146 318 773.1 0 318 147 64 773.1 0 64 148 74 773.1 0 74 149 68 773.1 0 68 150 92 773.1 0 92 151 44 773.1 0 44 152 52 773.1 0 52 153 45 773.1 0 45 154 39 773.1 0 39 155 36 773.1 0 36 156 34 773.1 0 34 157 37 773.1 0 37 158 44 773.1 0 44 159 41 773.1 0 41 160 38 773.1 0 38 161 47 773.1 0 47 162 44 773.1 0 44 163 40 773.1 0 40 164 52 773.1 0 52 165 55 773.1 0 55 166 56 773.1 0 56 167 70 773.1 0 70 168 361 773.1 0 361 169 126 773.1 0 126 170 42 773.1 0 42 171 65 773.1 0 65 172 61 773.1 0 61 173 83 773.1 0 83 174 105 773.1 0 105 175 269 773.1 0 269 176 84 773.1 0 84 177 91 773.1 0 91 178 97 773.1 0 97 179 64 773.1 0 64 180 80 773.1 0 80 181 122 773.1 0 122 182 125 773.1 0 125 183 98 773.1 0 98 184 136 773.1 0 136 185 238 773.1 0 238 186 266 773.1 0 266 187 299 773.1 0 299 188 3787 773.1 0 3787 189 8838 773.1 0 8838 190 9639 773.1 0 9639 191 642 773.1 0 642 192 280 773.1 0 280 193 219 773.1 0 219 194 144 773.1 0 144 195 79 773.1 0 79 196 101 773.1 0 101 197 45 773.1 0 45 198 35 773.1 0 35 199 53 773.1 0 53 200 378 773.1 0 378 201 51 773.1 0 51 202 40 773.1 0 40 203 52 773.1 0 52 204 53 773.1 0 53 205 73 773.1 0 73 206 878 773.1 0 878 207 825 773.1 0 825 208 1367 773.1 0 1367 209 222 773.1 0 222 210 43 773.1 0 43 211 139 773.1 0 139 212 178 773.1 0 178 213 262 773.1 0 262 214 80 773.1 0 80 215 42 773.1 0 42 216 28 773.1 0 28 217 51 773.1 0 51 218 69 773.1 0 69 219 40 773.1 0 40 220 40 773.1 0 40 221 43 773.1 0 43 222 42 773.1 0 42 223 31 773.1 0 31 224 37 773.1 0 37 225 42 773.1 0 42 226 45 773.1 0 45 227 66 773.1 0 66 228 47 773.1 0 47 229 65 773.1 0 65 230 62 773.1 0 62 231 66 773.1 0 66 232 41 773.1 0 41 233 46 773.1 0 46 234 32 773.1 0 32 235 45 773.1 0 45 236 47 773.1 0 47 237 57 773.1 0 57 238 37 773.1 0 37 239 38 773.1 0 38 240 44 773.1 0 44 241 55 773.1 0 55 242 55 773.1 0 55 243 38 773.1 0 38 244 41 773.1 0 41 245 39 773.1 0 39 246 37 773.1 0 37 247 65 773.1 0 65 248 65 773.1 0 65 249 36 773.1 0 36 250 65 773.1 0 65 251 68 773.1 0 68 252 74 773.1 0 74 253 95 773.1 0 95 254 54 773.1 0 54 255 61 773.1 0 61 256 115 773.1 0 115 257 104 773.1 0 104 258 85 773.1 0 85 259 78 773.1 0 78 260 49 773.1 0 49 261 63 773.1 0 63 262 64 773.1 0 64 263 60 773.1 0 60 264 50 773.1 0 50 265 57 773.1 0 57 266 84 773.1 0 84 267 77 773.1 0 77 268 134 773.1 0 134 269 217 773.1 0 217 270 187 773.1 0 187 271 2034 773.1 0 2034 272 2260 773.1 0 2260 273 2457 773.1 0 2457 274 383 773.1 0 383 275 52 773.1 0 52 276 65 773.1 0 65 277 76 773.1 0 76 278 68 773.1 0 68 279 56 773.1 0 56 280 80 773.1 0 80 281 74 773.1 0 74 282 568 773.1 0 568 283 641 773.1 0 641 284 839 773.1 0 839 285 59 773.1 0 59 286 56 773.1 0 56 287 61 773.1 0 61 288 111 773.1 0 111 289 75 773.1 0 75 290 54 773.1 0 54 291 108 773.1 0 108 292 348 773.1 0 348 293 342 773.1 0 342 294 441 773.1 0 441 295 141 773.1 0 141 296 91 773.1 0 91 297 79 773.1 0 79 298 85 773.1 0 85 299 62 773.1 0 62 300 149 773.1 0 149 301 254 773.1 0 254 Finished in 17.76 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 238,842 Reads with adapters: 79,882 (33.4%) Reads that were too short: 97 (0.0%) Reads written (passing filters): 79,785 (33.4%) Total basepairs processed: 60,394,652 bp Total written (filtered): 21,778,735 bp (36.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79882 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 0 8 18 16 0.0 3 0 0 16 19 636 0.0 3 0 616 13 6 1 20 78842 0.0 4 77570 1223 45 3 1 21 191 0.0 4 30 158 2 0 1 22 13 0.0 4 2 0 10 1 23 42 0.0 4 0 0 0 34 8 27 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 55 4 0.0 4 0 0 0 0 4 62 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 92 1 0.0 4 1 93 1 0.0 4 1 109 2 0.0 4 0 0 0 0 2 127 4 0.0 4 0 0 0 0 4 130 18 0.0 4 0 0 0 0 18 138 5 0.0 4 0 0 0 0 5 139 4 0.0 4 0 0 0 0 4 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 40 0.0 4 0 0 0 0 40 156 20 0.0 4 0 0 0 3 17 157 3 0.0 4 0 0 0 0 3 161 1 0.0 4 0 0 0 0 1 162 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 1 197 1 0.0 4 1 204 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 1 263 1 0.0 4 0 0 0 0 1 282 5 0.0 4 0 0 0 0 5 284 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.53 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 79,785 Reads with adapters: 5,348 (6.7%) Reads written (passing filters): 79,785 (100.0%) Total basepairs processed: 21,778,735 bp Total written (filtered): 21,720,519 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5348 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.0% G: 94.5% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 1246.6 0 27 4 7 311.7 0 7 5 2 77.9 0 2 8 3 1.2 0 3 9 90 0.3 0 2 88 10 1881 0.1 1 57 1824 11 3318 0.0 1 29 3289 12 4 0.0 1 1 3 13 2 0.0 1 0 2 14 1 0.0 1 0 1 15 1 0.0 1 0 1 123 5 0.0 1 5 151 4 0.0 1 2 2 200 1 0.0 1 1 209 1 0.0 1 0 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.