This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 148.94 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 195,896 (6.2%) Reads that were too short: 69,247 (2.2%) Reads written (passing filters): 126,649 (4.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 22,971,663 bp (2.4%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 195896 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 268 773.1 0 268 7 13025 773.1 0 13025 8 99 773.1 0 99 9 84 773.1 0 84 10 104 773.1 0 104 11 85 773.1 0 85 12 76 773.1 0 76 13 160 773.1 0 160 14 104 773.1 0 104 15 76 773.1 0 76 16 69 773.1 0 69 17 76 773.1 0 76 18 88 773.1 0 88 19 92 773.1 0 92 20 85 773.1 0 85 21 75 773.1 0 75 22 58 773.1 0 58 23 97 773.1 0 97 24 79 773.1 0 79 25 116 773.1 0 116 26 102 773.1 0 102 27 99 773.1 0 99 28 101 773.1 0 101 29 63 773.1 0 63 30 64 773.1 0 64 31 70 773.1 0 70 32 83 773.1 0 83 33 86 773.1 0 86 34 91 773.1 0 91 35 66 773.1 0 66 36 75 773.1 0 75 37 76 773.1 0 76 38 84 773.1 0 84 39 85 773.1 0 85 40 70 773.1 0 70 41 70 773.1 0 70 42 69 773.1 0 69 43 112 773.1 0 112 44 102 773.1 0 102 45 97 773.1 0 97 46 113 773.1 0 113 47 76 773.1 0 76 48 62 773.1 0 62 49 78 773.1 0 78 50 87 773.1 0 87 51 97 773.1 0 97 52 88 773.1 0 88 53 59 773.1 0 59 54 74 773.1 0 74 55 63 773.1 0 63 56 91 773.1 0 91 57 92 773.1 0 92 58 97 773.1 0 97 59 69 773.1 0 69 60 84 773.1 0 84 61 86 773.1 0 86 62 82 773.1 0 82 63 74 773.1 0 74 64 80 773.1 0 80 65 79 773.1 0 79 66 109 773.1 0 109 67 103 773.1 0 103 68 113 773.1 0 113 69 105 773.1 0 105 70 107 773.1 0 107 71 88 773.1 0 88 72 106 773.1 0 106 73 151 773.1 0 151 74 238 773.1 0 238 75 160 773.1 0 160 76 283 773.1 0 283 77 180 773.1 0 180 78 355 773.1 0 355 79 83 773.1 0 83 80 85 773.1 0 85 81 82 773.1 0 82 82 89 773.1 0 89 83 84 773.1 0 84 84 95 773.1 0 95 85 96 773.1 0 96 86 79 773.1 0 79 87 66 773.1 0 66 88 72 773.1 0 72 89 67 773.1 0 67 90 72 773.1 0 72 91 108 773.1 0 108 92 81 773.1 0 81 93 99 773.1 0 99 94 84 773.1 0 84 95 88 773.1 0 88 96 89 773.1 0 89 97 109 773.1 0 109 98 85 773.1 0 85 99 105 773.1 0 105 100 87 773.1 0 87 101 99 773.1 0 99 102 105 773.1 0 105 103 130 773.1 0 130 104 109 773.1 0 109 105 114 773.1 0 114 106 110 773.1 0 110 107 279 773.1 0 279 108 240 773.1 0 240 109 292 773.1 0 292 110 151 773.1 0 151 111 123 773.1 0 123 112 93 773.1 0 93 113 150 773.1 0 150 114 130 773.1 0 130 115 139 773.1 0 139 116 131 773.1 0 131 117 123 773.1 0 123 118 130 773.1 0 130 119 181 773.1 0 181 120 256 773.1 0 256 121 298 773.1 0 298 122 135 773.1 0 135 123 226 773.1 0 226 124 342 773.1 0 342 125 200 773.1 0 200 126 172 773.1 0 172 127 282 773.1 0 282 128 273 773.1 0 273 129 324 773.1 0 324 130 320 773.1 0 320 131 478 773.1 0 478 132 819 773.1 0 819 133 4602 773.1 0 4602 134 28934 773.1 0 28934 135 16697 773.1 0 16697 136 24844 773.1 0 24844 137 430 773.1 0 430 138 174 773.1 0 174 139 170 773.1 0 170 140 189 773.1 0 189 141 265 773.1 0 265 142 187 773.1 0 187 143 130 773.1 0 130 144 129 773.1 0 129 145 136 773.1 0 136 146 123 773.1 0 123 147 446 773.1 0 446 148 198 773.1 0 198 149 286 773.1 0 286 150 121 773.1 0 121 151 142 773.1 0 142 152 198 773.1 0 198 153 254 773.1 0 254 154 1409 773.1 0 1409 155 1366 773.1 0 1366 156 1125 773.1 0 1125 157 4133 773.1 0 4133 158 2223 773.1 0 2223 159 2729 773.1 0 2729 160 251 773.1 0 251 161 269 773.1 0 269 162 212 773.1 0 212 163 140 773.1 0 140 164 232 773.1 0 232 165 215 773.1 0 215 166 239 773.1 0 239 167 219 773.1 0 219 168 110 773.1 0 110 169 94 773.1 0 94 170 86 773.1 0 86 171 77 773.1 0 77 172 103 773.1 0 103 173 142 773.1 0 142 174 144 773.1 0 144 175 133 773.1 0 133 176 100 773.1 0 100 177 97 773.1 0 97 178 95 773.1 0 95 179 88 773.1 0 88 180 97 773.1 0 97 181 138 773.1 0 138 182 154 773.1 0 154 183 100 773.1 0 100 184 157 773.1 0 157 185 111 773.1 0 111 186 178 773.1 0 178 187 96 773.1 0 96 188 117 773.1 0 117 189 143 773.1 0 143 190 254 773.1 0 254 191 216 773.1 0 216 192 176 773.1 0 176 193 117 773.1 0 117 194 103 773.1 0 103 195 53 773.1 0 53 196 110 773.1 0 110 197 80 773.1 0 80 198 62 773.1 0 62 199 92 773.1 0 92 200 129 773.1 0 129 201 111 773.1 0 111 202 139 773.1 0 139 203 118 773.1 0 118 204 112 773.1 0 112 205 98 773.1 0 98 206 115 773.1 0 115 207 141 773.1 0 141 208 120 773.1 0 120 209 112 773.1 0 112 210 98 773.1 0 98 211 78 773.1 0 78 212 104 773.1 0 104 213 139 773.1 0 139 214 167 773.1 0 167 215 185 773.1 0 185 216 138 773.1 0 138 217 111 773.1 0 111 218 104 773.1 0 104 219 117 773.1 0 117 220 145 773.1 0 145 221 149 773.1 0 149 222 124 773.1 0 124 223 108 773.1 0 108 224 112 773.1 0 112 225 164 773.1 0 164 226 120 773.1 0 120 227 136 773.1 0 136 228 153 773.1 0 153 229 132 773.1 0 132 230 120 773.1 0 120 231 109 773.1 0 109 232 194 773.1 0 194 233 190 773.1 0 190 234 176 773.1 0 176 235 137 773.1 0 137 236 84 773.1 0 84 237 104 773.1 0 104 238 117 773.1 0 117 239 109 773.1 0 109 240 143 773.1 0 143 241 123 773.1 0 123 242 128 773.1 0 128 243 123 773.1 0 123 244 104 773.1 0 104 245 115 773.1 0 115 246 99 773.1 0 99 247 108 773.1 0 108 248 100 773.1 0 100 249 70 773.1 0 70 250 130 773.1 0 130 251 211 773.1 0 211 252 385 773.1 0 385 253 282 773.1 0 282 254 331 773.1 0 331 255 126 773.1 0 126 256 111 773.1 0 111 257 121 773.1 0 121 258 121 773.1 0 121 259 120 773.1 0 120 260 109 773.1 0 109 261 140 773.1 0 140 262 172 773.1 0 172 263 162 773.1 0 162 264 135 773.1 0 135 265 102 773.1 0 102 266 119 773.1 0 119 267 147 773.1 0 147 268 328 773.1 0 328 269 319 773.1 0 319 270 953 773.1 0 953 271 1044 773.1 0 1044 272 544 773.1 0 544 273 178 773.1 0 178 274 227 773.1 0 227 275 451 773.1 0 451 276 1777 773.1 0 1777 277 2147 773.1 0 2147 278 2826 773.1 0 2826 279 988 773.1 0 988 280 253 773.1 0 253 281 240 773.1 0 240 282 311 773.1 0 311 283 215 773.1 0 215 284 232 773.1 0 232 285 361 773.1 0 361 286 192 773.1 0 192 287 150 773.1 0 150 288 305 773.1 0 305 289 500 773.1 0 500 290 840 773.1 0 840 291 587 773.1 0 587 292 360 773.1 0 360 293 474 773.1 0 474 294 715 773.1 0 715 295 13304 773.1 0 13304 296 11820 773.1 0 11820 297 17055 773.1 0 17055 298 151 773.1 0 151 299 121 773.1 0 121 300 132 773.1 0 132 301 131 773.1 0 131 Finished in 7.15 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 126,649 Reads with adapters: 8,555 (6.8%) Reads that were too short: 139 (0.1%) Reads written (passing filters): 8,416 (6.6%) Total basepairs processed: 22,971,663 bp Total written (filtered): 2,122,380 bp (9.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8555 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 5 2 16 55 0.0 3 0 40 8 7 17 6994 0.0 3 5677 1186 111 20 18 16 0.0 3 3 7 6 19 25 0.0 3 5 2 8 10 20 27 0.0 3 1 0 0 26 22 1 0.0 3 0 0 0 1 24 1 0.0 3 1 27 4 0.0 3 0 0 0 4 32 268 0.0 3 0 0 1 267 33 3 0.0 3 0 0 1 2 35 60 0.0 3 0 0 4 56 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 4 0.0 3 0 0 0 4 58 912 0.0 3 0 0 1 911 59 8 0.0 3 0 0 0 8 61 5 0.0 3 0 0 0 5 63 3 0.0 3 0 0 0 3 68 12 0.0 3 0 0 0 12 71 3 0.0 3 0 0 1 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 13 0.0 3 0 1 1 11 84 2 0.0 3 0 0 0 2 92 60 0.0 3 0 0 0 60 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 6 0.0 3 0 0 0 6 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 1 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 1 2 172 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 1 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 1 1 221 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 8,416 Reads with adapters: 50 (0.6%) Reads written (passing filters): 8,416 (100.0%) Total basepairs processed: 2,122,380 bp Total written (filtered): 2,122,222 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 50 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.0% C: 32.0% G: 30.0% T: 32.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 45 131.5 0 45 4 2 32.9 0 2 5 3 8.2 0 3