This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 150.76 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 217,013 (6.9%) Reads that were too short: 88,134 (2.8%) Reads written (passing filters): 128,879 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,559,763 bp (2.5%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 217013 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 241 773.1 0 241 7 13204 773.1 0 13204 8 132 773.1 0 132 9 120 773.1 0 120 10 157 773.1 0 157 11 118 773.1 0 118 12 106 773.1 0 106 13 152 773.1 0 152 14 130 773.1 0 130 15 106 773.1 0 106 16 129 773.1 0 129 17 92 773.1 0 92 18 124 773.1 0 124 19 115 773.1 0 115 20 124 773.1 0 124 21 124 773.1 0 124 22 102 773.1 0 102 23 139 773.1 0 139 24 114 773.1 0 114 25 118 773.1 0 118 26 121 773.1 0 121 27 126 773.1 0 126 28 92 773.1 0 92 29 95 773.1 0 95 30 111 773.1 0 111 31 101 773.1 0 101 32 115 773.1 0 115 33 134 773.1 0 134 34 131 773.1 0 131 35 137 773.1 0 137 36 105 773.1 0 105 37 108 773.1 0 108 38 91 773.1 0 91 39 113 773.1 0 113 40 96 773.1 0 96 41 90 773.1 0 90 42 109 773.1 0 109 43 111 773.1 0 111 44 93 773.1 0 93 45 96 773.1 0 96 46 104 773.1 0 104 47 110 773.1 0 110 48 113 773.1 0 113 49 93 773.1 0 93 50 129 773.1 0 129 51 105 773.1 0 105 52 100 773.1 0 100 53 116 773.1 0 116 54 100 773.1 0 100 55 101 773.1 0 101 56 123 773.1 0 123 57 127 773.1 0 127 58 117 773.1 0 117 59 96 773.1 0 96 60 121 773.1 0 121 61 123 773.1 0 123 62 110 773.1 0 110 63 123 773.1 0 123 64 136 773.1 0 136 65 101 773.1 0 101 66 107 773.1 0 107 67 133 773.1 0 133 68 131 773.1 0 131 69 191 773.1 0 191 70 134 773.1 0 134 71 106 773.1 0 106 72 158 773.1 0 158 73 134 773.1 0 134 74 244 773.1 0 244 75 176 773.1 0 176 76 246 773.1 0 246 77 228 773.1 0 228 78 306 773.1 0 306 79 126 773.1 0 126 80 147 773.1 0 147 81 139 773.1 0 139 82 139 773.1 0 139 83 126 773.1 0 126 84 163 773.1 0 163 85 151 773.1 0 151 86 108 773.1 0 108 87 138 773.1 0 138 88 131 773.1 0 131 89 151 773.1 0 151 90 138 773.1 0 138 91 151 773.1 0 151 92 118 773.1 0 118 93 137 773.1 0 137 94 120 773.1 0 120 95 144 773.1 0 144 96 150 773.1 0 150 97 137 773.1 0 137 98 134 773.1 0 134 99 132 773.1 0 132 100 117 773.1 0 117 101 135 773.1 0 135 102 114 773.1 0 114 103 172 773.1 0 172 104 140 773.1 0 140 105 147 773.1 0 147 106 159 773.1 0 159 107 272 773.1 0 272 108 225 773.1 0 225 109 299 773.1 0 299 110 190 773.1 0 190 111 168 773.1 0 168 112 130 773.1 0 130 113 181 773.1 0 181 114 178 773.1 0 178 115 154 773.1 0 154 116 141 773.1 0 141 117 180 773.1 0 180 118 193 773.1 0 193 119 183 773.1 0 183 120 300 773.1 0 300 121 341 773.1 0 341 122 216 773.1 0 216 123 244 773.1 0 244 124 306 773.1 0 306 125 218 773.1 0 218 126 233 773.1 0 233 127 275 773.1 0 275 128 260 773.1 0 260 129 348 773.1 0 348 130 319 773.1 0 319 131 488 773.1 0 488 132 669 773.1 0 669 133 4411 773.1 0 4411 134 27339 773.1 0 27339 135 15821 773.1 0 15821 136 24433 773.1 0 24433 137 370 773.1 0 370 138 215 773.1 0 215 139 210 773.1 0 210 140 175 773.1 0 175 141 220 773.1 0 220 142 194 773.1 0 194 143 149 773.1 0 149 144 138 773.1 0 138 145 155 773.1 0 155 146 168 773.1 0 168 147 309 773.1 0 309 148 201 773.1 0 201 149 250 773.1 0 250 150 168 773.1 0 168 151 134 773.1 0 134 152 203 773.1 0 203 153 262 773.1 0 262 154 1417 773.1 0 1417 155 1383 773.1 0 1383 156 1165 773.1 0 1165 157 4714 773.1 0 4714 158 2668 773.1 0 2668 159 3073 773.1 0 3073 160 201 773.1 0 201 161 175 773.1 0 175 162 175 773.1 0 175 163 125 773.1 0 125 164 154 773.1 0 154 165 180 773.1 0 180 166 171 773.1 0 171 167 203 773.1 0 203 168 124 773.1 0 124 169 152 773.1 0 152 170 103 773.1 0 103 171 123 773.1 0 123 172 122 773.1 0 122 173 136 773.1 0 136 174 172 773.1 0 172 175 140 773.1 0 140 176 145 773.1 0 145 177 116 773.1 0 116 178 133 773.1 0 133 179 97 773.1 0 97 180 101 773.1 0 101 181 152 773.1 0 152 182 151 773.1 0 151 183 118 773.1 0 118 184 123 773.1 0 123 185 116 773.1 0 116 186 160 773.1 0 160 187 131 773.1 0 131 188 124 773.1 0 124 189 115 773.1 0 115 190 280 773.1 0 280 191 210 773.1 0 210 192 209 773.1 0 209 193 137 773.1 0 137 194 111 773.1 0 111 195 97 773.1 0 97 196 136 773.1 0 136 197 94 773.1 0 94 198 111 773.1 0 111 199 123 773.1 0 123 200 125 773.1 0 125 201 124 773.1 0 124 202 142 773.1 0 142 203 128 773.1 0 128 204 117 773.1 0 117 205 131 773.1 0 131 206 142 773.1 0 142 207 116 773.1 0 116 208 116 773.1 0 116 209 90 773.1 0 90 210 94 773.1 0 94 211 107 773.1 0 107 212 111 773.1 0 111 213 147 773.1 0 147 214 162 773.1 0 162 215 144 773.1 0 144 216 133 773.1 0 133 217 127 773.1 0 127 218 123 773.1 0 123 219 114 773.1 0 114 220 128 773.1 0 128 221 152 773.1 0 152 222 123 773.1 0 123 223 94 773.1 0 94 224 131 773.1 0 131 225 200 773.1 0 200 226 155 773.1 0 155 227 120 773.1 0 120 228 138 773.1 0 138 229 107 773.1 0 107 230 111 773.1 0 111 231 128 773.1 0 128 232 130 773.1 0 130 233 135 773.1 0 135 234 126 773.1 0 126 235 129 773.1 0 129 236 98 773.1 0 98 237 111 773.1 0 111 238 130 773.1 0 130 239 116 773.1 0 116 240 132 773.1 0 132 241 128 773.1 0 128 242 106 773.1 0 106 243 114 773.1 0 114 244 127 773.1 0 127 245 114 773.1 0 114 246 118 773.1 0 118 247 123 773.1 0 123 248 99 773.1 0 99 249 102 773.1 0 102 250 120 773.1 0 120 251 192 773.1 0 192 252 310 773.1 0 310 253 271 773.1 0 271 254 250 773.1 0 250 255 91 773.1 0 91 256 97 773.1 0 97 257 128 773.1 0 128 258 119 773.1 0 119 259 126 773.1 0 126 260 124 773.1 0 124 261 113 773.1 0 113 262 144 773.1 0 144 263 102 773.1 0 102 264 136 773.1 0 136 265 111 773.1 0 111 266 123 773.1 0 123 267 119 773.1 0 119 268 229 773.1 0 229 269 256 773.1 0 256 270 951 773.1 0 951 271 1080 773.1 0 1080 272 587 773.1 0 587 273 168 773.1 0 168 274 234 773.1 0 234 275 418 773.1 0 418 276 2215 773.1 0 2215 277 2464 773.1 0 2464 278 3413 773.1 0 3413 279 1018 773.1 0 1018 280 214 773.1 0 214 281 256 773.1 0 256 282 366 773.1 0 366 283 197 773.1 0 197 284 237 773.1 0 237 285 466 773.1 0 466 286 201 773.1 0 201 287 185 773.1 0 185 288 396 773.1 0 396 289 629 773.1 0 629 290 921 773.1 0 921 291 637 773.1 0 637 292 486 773.1 0 486 293 533 773.1 0 533 294 786 773.1 0 786 295 18329 773.1 0 18329 296 16723 773.1 0 16723 297 24419 773.1 0 24419 298 178 773.1 0 178 299 91 773.1 0 91 300 106 773.1 0 106 301 100 773.1 0 100 Finished in 7.84 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 128,879 Reads with adapters: 7,308 (5.7%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 7,289 (5.7%) Total basepairs processed: 23,559,763 bp Total written (filtered): 1,993,144 bp (8.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7308 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 42 0.0 3 0 37 3 1 1 20 7191 0.0 4 6565 606 20 21 7 0.0 4 2 5 22 3 0.0 4 2 0 0 0 1 23 2 0.0 4 0 0 0 2 24 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 1 48 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 81 3 0.0 4 0 0 0 0 3 82 1 0.0 4 0 0 0 0 1 90 1 0.0 4 1 102 7 0.0 4 0 0 0 0 7 104 1 0.0 4 0 0 0 0 1 127 36 0.0 4 0 0 0 0 36 135 1 0.0 4 0 0 0 0 1 215 1 0.0 4 1 220 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 7,289 Reads with adapters: 107 (1.5%) Reads written (passing filters): 7,289 (100.0%) Total basepairs processed: 1,993,144 bp Total written (filtered): 1,992,002 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 107 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 3.7% G: 91.6% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 113.9 0 4 4 1 28.5 0 1 10 1 0.0 1 0 1 11 96 0.0 1 0 96 12 5 0.0 1 0 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.