This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 155.15 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 400,699 (12.7%) Reads that were too short: 13,580 (0.4%) Reads written (passing filters): 387,119 (12.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 109,907,310 bp (11.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 400699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3213 773.1 0 3213 7 5584 773.1 0 5584 8 203410 773.1 0 203410 9 145392 773.1 0 145392 10 140 773.1 0 140 11 9 773.1 0 9 12 4 773.1 0 4 13 30 773.1 0 30 14 107 773.1 0 107 15 126 773.1 0 126 16 32 773.1 0 32 17 9 773.1 0 9 18 49 773.1 0 49 19 38 773.1 0 38 20 30 773.1 0 30 21 4 773.1 0 4 22 1 773.1 0 1 23 4 773.1 0 4 24 2 773.1 0 2 25 4 773.1 0 4 26 8 773.1 0 8 27 17 773.1 0 17 28 10 773.1 0 10 29 5 773.1 0 5 30 3 773.1 0 3 31 2 773.1 0 2 32 5 773.1 0 5 33 6 773.1 0 6 34 4 773.1 0 4 35 10 773.1 0 10 36 4 773.1 0 4 38 4 773.1 0 4 39 6 773.1 0 6 40 12 773.1 0 12 41 16 773.1 0 16 42 6 773.1 0 6 43 92 773.1 0 92 44 272 773.1 0 272 45 341 773.1 0 341 46 93 773.1 0 93 47 11 773.1 0 11 48 7 773.1 0 7 49 5 773.1 0 5 50 33 773.1 0 33 51 7 773.1 0 7 52 18 773.1 0 18 53 14 773.1 0 14 54 11 773.1 0 11 55 4 773.1 0 4 56 9 773.1 0 9 57 11 773.1 0 11 58 3 773.1 0 3 59 48 773.1 0 48 60 42 773.1 0 42 61 20 773.1 0 20 62 20 773.1 0 20 63 16 773.1 0 16 64 34 773.1 0 34 65 132 773.1 0 132 66 42 773.1 0 42 67 64 773.1 0 64 68 48 773.1 0 48 69 40 773.1 0 40 70 23 773.1 0 23 71 23 773.1 0 23 72 45 773.1 0 45 73 73 773.1 0 73 74 79 773.1 0 79 75 110 773.1 0 110 76 352 773.1 0 352 77 4058 773.1 0 4058 78 2524 773.1 0 2524 79 5887 773.1 0 5887 80 45 773.1 0 45 81 11 773.1 0 11 82 42 773.1 0 42 83 15 773.1 0 15 84 23 773.1 0 23 85 12 773.1 0 12 86 34 773.1 0 34 87 2 773.1 0 2 88 5 773.1 0 5 89 4 773.1 0 4 90 10 773.1 0 10 91 13 773.1 0 13 92 40 773.1 0 40 93 75 773.1 0 75 94 23 773.1 0 23 95 11 773.1 0 11 96 26 773.1 0 26 97 7 773.1 0 7 98 20 773.1 0 20 99 12 773.1 0 12 100 5 773.1 0 5 101 11 773.1 0 11 102 4 773.1 0 4 103 10 773.1 0 10 104 4 773.1 0 4 105 5 773.1 0 5 106 3 773.1 0 3 107 6 773.1 0 6 108 4 773.1 0 4 109 3 773.1 0 3 110 10 773.1 0 10 111 4 773.1 0 4 112 10 773.1 0 10 113 17 773.1 0 17 114 41 773.1 0 41 115 52 773.1 0 52 116 102 773.1 0 102 117 7 773.1 0 7 118 5 773.1 0 5 119 5 773.1 0 5 120 13 773.1 0 13 121 19 773.1 0 19 122 22 773.1 0 22 123 25 773.1 0 25 124 12 773.1 0 12 125 16 773.1 0 16 126 18 773.1 0 18 127 29 773.1 0 29 128 26 773.1 0 26 129 18 773.1 0 18 130 64 773.1 0 64 131 13 773.1 0 13 132 18 773.1 0 18 133 15 773.1 0 15 134 34 773.1 0 34 135 92 773.1 0 92 136 152 773.1 0 152 137 296 773.1 0 296 138 209 773.1 0 209 139 173 773.1 0 173 140 54 773.1 0 54 141 41 773.1 0 41 142 48 773.1 0 48 143 33 773.1 0 33 144 18 773.1 0 18 145 11 773.1 0 11 146 14 773.1 0 14 147 13 773.1 0 13 148 19 773.1 0 19 149 28 773.1 0 28 150 29 773.1 0 29 151 14 773.1 0 14 152 9 773.1 0 9 153 16 773.1 0 16 154 21 773.1 0 21 155 24 773.1 0 24 156 9 773.1 0 9 157 22 773.1 0 22 158 55 773.1 0 55 159 10 773.1 0 10 160 9 773.1 0 9 161 17 773.1 0 17 162 21 773.1 0 21 163 42 773.1 0 42 164 59 773.1 0 59 165 79 773.1 0 79 166 35 773.1 0 35 167 8 773.1 0 8 168 19 773.1 0 19 169 19 773.1 0 19 170 30 773.1 0 30 171 42 773.1 0 42 172 54 773.1 0 54 173 560 773.1 0 560 174 410 773.1 0 410 175 613 773.1 0 613 176 80 773.1 0 80 177 27 773.1 0 27 178 31 773.1 0 31 179 90 773.1 0 90 180 544 773.1 0 544 181 308 773.1 0 308 182 306 773.1 0 306 183 26 773.1 0 26 184 29 773.1 0 29 185 57 773.1 0 57 186 38 773.1 0 38 187 66 773.1 0 66 188 65 773.1 0 65 189 105 773.1 0 105 190 411 773.1 0 411 191 2531 773.1 0 2531 192 2721 773.1 0 2721 193 1800 773.1 0 1800 194 308 773.1 0 308 195 13 773.1 0 13 196 7 773.1 0 7 197 23 773.1 0 23 198 32 773.1 0 32 199 35 773.1 0 35 200 20 773.1 0 20 201 22 773.1 0 22 202 173 773.1 0 173 203 492 773.1 0 492 204 452 773.1 0 452 205 251 773.1 0 251 206 18 773.1 0 18 207 21 773.1 0 21 208 149 773.1 0 149 209 147 773.1 0 147 210 190 773.1 0 190 211 53 773.1 0 53 212 13 773.1 0 13 213 12 773.1 0 12 214 17 773.1 0 17 215 57 773.1 0 57 216 49 773.1 0 49 217 316 773.1 0 316 218 43 773.1 0 43 219 24 773.1 0 24 220 30 773.1 0 30 221 6 773.1 0 6 222 9 773.1 0 9 223 14 773.1 0 14 224 17 773.1 0 17 225 22 773.1 0 22 226 30 773.1 0 30 227 40 773.1 0 40 228 23 773.1 0 23 229 44 773.1 0 44 230 12 773.1 0 12 231 11 773.1 0 11 232 38 773.1 0 38 233 32 773.1 0 32 234 61 773.1 0 61 235 38 773.1 0 38 236 45 773.1 0 45 237 22 773.1 0 22 238 9 773.1 0 9 239 21 773.1 0 21 240 44 773.1 0 44 241 14 773.1 0 14 242 61 773.1 0 61 243 26 773.1 0 26 244 30 773.1 0 30 245 31 773.1 0 31 246 14 773.1 0 14 247 47 773.1 0 47 248 47 773.1 0 47 249 13 773.1 0 13 250 28 773.1 0 28 251 75 773.1 0 75 252 251 773.1 0 251 253 300 773.1 0 300 254 239 773.1 0 239 255 98 773.1 0 98 256 13 773.1 0 13 257 35 773.1 0 35 258 303 773.1 0 303 259 370 773.1 0 370 260 423 773.1 0 423 261 620 773.1 0 620 262 501 773.1 0 501 263 407 773.1 0 407 264 258 773.1 0 258 265 293 773.1 0 293 266 111 773.1 0 111 267 45 773.1 0 45 268 22 773.1 0 22 269 17 773.1 0 17 270 36 773.1 0 36 271 54 773.1 0 54 272 96 773.1 0 96 273 85 773.1 0 85 274 59 773.1 0 59 275 56 773.1 0 56 276 41 773.1 0 41 277 58 773.1 0 58 278 71 773.1 0 71 279 101 773.1 0 101 280 89 773.1 0 89 281 52 773.1 0 52 282 42 773.1 0 42 283 190 773.1 0 190 284 1404 773.1 0 1404 285 964 773.1 0 964 286 1618 773.1 0 1618 287 78 773.1 0 78 288 25 773.1 0 25 289 33 773.1 0 33 290 39 773.1 0 39 291 46 773.1 0 46 292 35 773.1 0 35 293 22 773.1 0 22 294 41 773.1 0 41 295 73 773.1 0 73 296 105 773.1 0 105 297 83 773.1 0 83 298 98 773.1 0 98 299 28 773.1 0 28 300 31 773.1 0 31 301 90 773.1 0 90 Finished in 28.50 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 387,119 Reads with adapters: 198,811 (51.4%) Reads that were too short: 138 (0.0%) Reads written (passing filters): 198,673 (51.3%) Total basepairs processed: 109,907,310 bp Total written (filtered): 54,812,049 bp (49.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 198811 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 22 0.0 2 0 0 0 22 15 81 0.0 3 0 0 44 37 16 1398 0.0 3 0 1169 152 77 17 194533 0.0 3 184812 8086 1354 281 18 739 0.0 3 78 372 52 237 19 337 0.0 3 2 8 193 134 20 1423 0.0 3 776 33 5 609 21 23 0.0 3 1 1 4 17 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 24 1 0.0 3 1 27 1 0.0 3 1 29 1 0.0 3 0 0 1 30 11 0.0 3 0 0 0 11 31 1 0.0 3 0 0 0 1 32 1 0.0 3 1 33 3 0.0 3 0 0 1 2 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 57 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 4 0.0 3 1 0 0 3 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 5 0.0 3 4 0 1 82 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 109 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 1 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 1 124 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 1 3 128 2 0.0 3 0 0 0 2 130 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 157 2 0.0 3 1 0 0 1 159 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 6 0.0 3 0 0 0 6 184 4 0.0 3 0 0 0 4 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 12 0.0 3 0 0 0 12 193 2 0.0 3 0 0 0 2 194 12 0.0 3 0 0 1 11 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 1 270 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 0 3 279 11 0.0 3 0 0 0 11 280 6 0.0 3 0 0 0 6 281 10 0.0 3 0 0 0 10 282 24 0.0 3 0 0 0 24 283 1 0.0 3 0 0 0 1 285 3 0.0 3 0 0 0 3 287 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 11.35 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 198,673 Reads with adapters: 1,137 (0.6%) Reads written (passing filters): 198,673 (100.0%) Total basepairs processed: 54,812,049 bp Total written (filtered): 54,808,242 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1137 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.2% C: 11.2% G: 44.4% T: 8.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 777 3104.3 0 777 4 337 776.1 0 337 5 19 194.0 0 19 6 1 48.5 0 1 7 2 12.1 0 2 13 1 0.0 1 0 1