This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 155.16 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 404,954 (12.8%) Reads that were too short: 6,957 (0.2%) Reads written (passing filters): 397,997 (12.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 113,399,937 bp (11.9%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 404954 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2951 773.1 0 2951 7 4478 773.1 0 4478 8 184516 773.1 0 184516 9 178994 773.1 0 178994 10 143 773.1 0 143 11 11 773.1 0 11 12 4 773.1 0 4 13 7 773.1 0 7 14 5 773.1 0 5 15 5 773.1 0 5 16 3 773.1 0 3 17 2 773.1 0 2 18 10 773.1 0 10 19 16 773.1 0 16 20 11 773.1 0 11 21 6 773.1 0 6 22 3 773.1 0 3 24 2 773.1 0 2 25 1 773.1 0 1 26 2 773.1 0 2 27 2 773.1 0 2 28 1 773.1 0 1 29 4 773.1 0 4 30 2 773.1 0 2 31 3 773.1 0 3 33 6 773.1 0 6 34 1 773.1 0 1 35 18 773.1 0 18 36 2 773.1 0 2 37 3 773.1 0 3 38 1 773.1 0 1 39 3 773.1 0 3 40 16 773.1 0 16 41 7 773.1 0 7 42 8 773.1 0 8 43 8 773.1 0 8 44 45 773.1 0 45 45 65 773.1 0 65 46 33 773.1 0 33 47 7 773.1 0 7 48 4 773.1 0 4 49 5 773.1 0 5 50 24 773.1 0 24 51 9 773.1 0 9 52 8 773.1 0 8 53 5 773.1 0 5 54 6 773.1 0 6 55 3 773.1 0 3 56 7 773.1 0 7 57 15 773.1 0 15 58 8 773.1 0 8 59 18 773.1 0 18 60 19 773.1 0 19 61 7 773.1 0 7 62 17 773.1 0 17 63 21 773.1 0 21 64 44 773.1 0 44 65 135 773.1 0 135 66 22 773.1 0 22 67 31 773.1 0 31 68 19 773.1 0 19 69 12 773.1 0 12 70 14 773.1 0 14 71 27 773.1 0 27 72 47 773.1 0 47 73 73 773.1 0 73 74 74 773.1 0 74 75 154 773.1 0 154 76 320 773.1 0 320 77 4604 773.1 0 4604 78 2777 773.1 0 2777 79 6460 773.1 0 6460 80 19 773.1 0 19 81 6 773.1 0 6 82 44 773.1 0 44 83 8 773.1 0 8 84 8 773.1 0 8 85 5 773.1 0 5 86 8 773.1 0 8 87 5 773.1 0 5 88 5 773.1 0 5 89 2 773.1 0 2 90 13 773.1 0 13 91 16 773.1 0 16 92 22 773.1 0 22 93 34 773.1 0 34 94 10 773.1 0 10 95 12 773.1 0 12 96 30 773.1 0 30 97 9 773.1 0 9 98 24 773.1 0 24 99 8 773.1 0 8 100 10 773.1 0 10 101 9 773.1 0 9 102 5 773.1 0 5 103 1 773.1 0 1 104 3 773.1 0 3 105 9 773.1 0 9 106 3 773.1 0 3 107 8 773.1 0 8 108 7 773.1 0 7 109 4 773.1 0 4 110 10 773.1 0 10 111 6 773.1 0 6 112 8 773.1 0 8 113 11 773.1 0 11 114 15 773.1 0 15 115 15 773.1 0 15 116 78 773.1 0 78 117 9 773.1 0 9 118 1 773.1 0 1 119 7 773.1 0 7 120 6 773.1 0 6 121 12 773.1 0 12 122 10 773.1 0 10 123 13 773.1 0 13 124 6 773.1 0 6 125 8 773.1 0 8 126 7 773.1 0 7 127 4 773.1 0 4 128 6 773.1 0 6 129 7 773.1 0 7 130 41 773.1 0 41 131 9 773.1 0 9 132 8 773.1 0 8 133 13 773.1 0 13 134 16 773.1 0 16 135 32 773.1 0 32 136 67 773.1 0 67 137 255 773.1 0 255 138 197 773.1 0 197 139 158 773.1 0 158 140 44 773.1 0 44 141 24 773.1 0 24 142 51 773.1 0 51 143 11 773.1 0 11 144 7 773.1 0 7 145 6 773.1 0 6 146 3 773.1 0 3 147 7 773.1 0 7 148 10 773.1 0 10 149 12 773.1 0 12 150 14 773.1 0 14 151 8 773.1 0 8 152 12 773.1 0 12 153 6 773.1 0 6 154 12 773.1 0 12 155 9 773.1 0 9 156 13 773.1 0 13 157 7 773.1 0 7 158 43 773.1 0 43 159 4 773.1 0 4 160 14 773.1 0 14 161 11 773.1 0 11 162 12 773.1 0 12 163 24 773.1 0 24 164 30 773.1 0 30 165 25 773.1 0 25 166 23 773.1 0 23 167 9 773.1 0 9 168 10 773.1 0 10 169 7 773.1 0 7 170 7 773.1 0 7 171 20 773.1 0 20 172 14 773.1 0 14 173 280 773.1 0 280 174 240 773.1 0 240 175 271 773.1 0 271 176 29 773.1 0 29 177 12 773.1 0 12 178 25 773.1 0 25 179 72 773.1 0 72 180 383 773.1 0 383 181 193 773.1 0 193 182 151 773.1 0 151 183 10 773.1 0 10 184 24 773.1 0 24 185 18 773.1 0 18 186 21 773.1 0 21 187 40 773.1 0 40 188 27 773.1 0 27 189 93 773.1 0 93 190 336 773.1 0 336 191 2701 773.1 0 2701 192 2786 773.1 0 2786 193 1722 773.1 0 1722 194 201 773.1 0 201 195 14 773.1 0 14 196 2 773.1 0 2 197 8 773.1 0 8 198 9 773.1 0 9 199 16 773.1 0 16 200 10 773.1 0 10 201 18 773.1 0 18 202 162 773.1 0 162 203 282 773.1 0 282 204 327 773.1 0 327 205 158 773.1 0 158 206 12 773.1 0 12 207 9 773.1 0 9 208 36 773.1 0 36 209 38 773.1 0 38 210 28 773.1 0 28 211 14 773.1 0 14 212 4 773.1 0 4 213 9 773.1 0 9 214 4 773.1 0 4 215 38 773.1 0 38 216 18 773.1 0 18 217 318 773.1 0 318 218 18 773.1 0 18 219 9 773.1 0 9 220 12 773.1 0 12 221 2 773.1 0 2 222 2 773.1 0 2 223 6 773.1 0 6 224 4 773.1 0 4 225 14 773.1 0 14 226 7 773.1 0 7 227 13 773.1 0 13 228 19 773.1 0 19 229 8 773.1 0 8 230 4 773.1 0 4 231 8 773.1 0 8 232 4 773.1 0 4 233 16 773.1 0 16 234 27 773.1 0 27 235 20 773.1 0 20 236 24 773.1 0 24 237 13 773.1 0 13 238 8 773.1 0 8 239 8 773.1 0 8 240 8 773.1 0 8 241 7 773.1 0 7 242 16 773.1 0 16 243 11 773.1 0 11 244 15 773.1 0 15 245 8 773.1 0 8 246 10 773.1 0 10 247 14 773.1 0 14 248 14 773.1 0 14 249 14 773.1 0 14 250 19 773.1 0 19 251 49 773.1 0 49 252 153 773.1 0 153 253 135 773.1 0 135 254 105 773.1 0 105 255 24 773.1 0 24 256 14 773.1 0 14 257 24 773.1 0 24 258 265 773.1 0 265 259 333 773.1 0 333 260 225 773.1 0 225 261 400 773.1 0 400 262 238 773.1 0 238 263 265 773.1 0 265 264 210 773.1 0 210 265 133 773.1 0 133 266 39 773.1 0 39 267 14 773.1 0 14 268 14 773.1 0 14 269 3 773.1 0 3 270 8 773.1 0 8 271 23 773.1 0 23 272 31 773.1 0 31 273 36 773.1 0 36 274 21 773.1 0 21 275 20 773.1 0 20 276 8 773.1 0 8 277 26 773.1 0 26 278 27 773.1 0 27 279 29 773.1 0 29 280 31 773.1 0 31 281 21 773.1 0 21 282 15 773.1 0 15 283 80 773.1 0 80 284 573 773.1 0 573 285 403 773.1 0 403 286 761 773.1 0 761 287 31 773.1 0 31 288 27 773.1 0 27 289 23 773.1 0 23 290 27 773.1 0 27 291 27 773.1 0 27 292 17 773.1 0 17 293 6 773.1 0 6 294 12 773.1 0 12 295 31 773.1 0 31 296 51 773.1 0 51 297 38 773.1 0 38 298 20 773.1 0 20 299 10 773.1 0 10 300 4 773.1 0 4 301 38 773.1 0 38 Finished in 30.81 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 397,997 Reads with adapters: 192,143 (48.3%) Reads that were too short: 65 (0.0%) Reads written (passing filters): 192,078 (48.3%) Total basepairs processed: 113,399,937 bp Total written (filtered): 52,234,683 bp (46.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 192143 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 3 0.0 3 0 0 0 0 3 18 67 0.0 3 0 0 64 3 19 2620 0.0 3 0 2563 44 4 9 20 188425 0.0 4 185333 2978 99 13 2 21 531 0.0 4 159 352 18 1 1 22 40 0.0 4 5 2 29 2 2 23 128 0.0 4 5 2 0 102 19 24 4 0.0 4 0 0 0 1 3 25 1 0.0 4 0 0 0 0 1 27 3 0.0 4 0 1 0 0 2 29 1 0.0 4 1 34 1 0.0 4 0 1 48 2 0.0 4 0 0 0 0 2 80 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 218 0.0 4 0 0 0 0 218 128 2 0.0 4 0 0 0 0 2 129 2 0.0 4 0 0 0 0 2 137 1 0.0 4 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 172 12 0.0 4 0 0 0 0 12 179 1 0.0 4 1 186 1 0.0 4 1 191 2 0.0 4 0 0 0 0 2 192 1 0.0 4 1 193 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 211 3 0.0 4 3 212 1 0.0 4 1 219 3 0.0 4 2 0 0 0 1 220 1 0.0 4 1 224 1 0.0 4 0 1 225 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 1 2 230 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 1 239 1 0.0 4 1 240 1 0.0 4 0 1 241 1 0.0 4 1 245 1 0.0 4 1 249 6 0.0 4 0 0 1 0 5 252 1 0.0 4 1 253 1 0.0 4 1 255 1 0.0 4 0 0 0 0 1 262 2 0.0 4 1 0 0 0 1 263 2 0.0 4 0 0 1 0 1 264 1 0.0 4 0 0 0 1 265 3 0.0 4 0 0 0 1 2 276 1 0.0 4 0 0 0 1 277 1 0.0 4 1 279 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 1 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 1 288 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 1 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 11.26 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 192,078 Reads with adapters: 28,045 (14.6%) Reads written (passing filters): 192,078 (100.0%) Total basepairs processed: 52,234,683 bp Total written (filtered): 51,949,415 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 28045 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 2.3% G: 95.7% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 36 3001.2 0 36 4 26 750.3 0 26 5 12 187.6 0 12 6 14 46.9 0 14 7 4 11.7 0 4 8 13 2.9 0 13 9 17187 0.7 0 365 16822 10 8534 0.2 1 252 8282 11 2007 0.0 1 21 1986 12 27 0.0 1 1 26 13 15 0.0 1 1 14 14 1 0.0 1 1 17 9 0.0 1 5 4 18 2 0.0 1 1 1 19 2 0.0 1 2 22 15 0.0 1 14 1 23 3 0.0 1 3 34 3 0.0 1 3 36 1 0.0 1 1 39 2 0.0 1 2 63 2 0.0 1 2 92 1 0.0 1 1 94 3 0.0 1 3 105 1 0.0 1 1 112 1 0.0 1 1 114 37 0.0 1 35 2 115 2 0.0 1 2 116 1 0.0 1 1 118 1 0.0 1 1 120 1 0.0 1 1 122 1 0.0 1 0 1 134 1 0.0 1 0 1 147 1 0.0 1 1 150 15 0.0 1 8 7 151 1 0.0 1 0 1 152 1 0.0 1 0 1 153 1 0.0 1 1 155 1 0.0 1 0 1 156 1 0.0 1 1 180 1 0.0 1 0 1 189 1 0.0 1 0 1 208 45 0.0 1 39 6 209 4 0.0 1 3 1 210 1 0.0 1 1 211 1 0.0 1 1 223 1 0.0 1 1 226 1 0.0 1 1 229 3 0.0 1 2 1 244 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.