This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.60 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 220,043 (6.9%) Reads that were too short: 11,299 (0.4%) Reads written (passing filters): 208,744 (6.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 60,240,832 bp (6.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 220043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1631 773.1 0 1631 7 107207 773.1 0 107207 8 89439 773.1 0 89439 9 63 773.1 0 63 10 15 773.1 0 15 11 7 773.1 0 7 12 4 773.1 0 4 13 14 773.1 0 14 14 17 773.1 0 17 15 15 773.1 0 15 16 14 773.1 0 14 17 13 773.1 0 13 18 57 773.1 0 57 19 65 773.1 0 65 20 62 773.1 0 62 21 7 773.1 0 7 22 10 773.1 0 10 23 19 773.1 0 19 24 14 773.1 0 14 25 12 773.1 0 12 26 28 773.1 0 28 27 35 773.1 0 35 28 44 773.1 0 44 29 32 773.1 0 32 30 17 773.1 0 17 31 13 773.1 0 13 32 11 773.1 0 11 33 41 773.1 0 41 34 27 773.1 0 27 35 41 773.1 0 41 36 196 773.1 0 196 37 166 773.1 0 166 38 602 773.1 0 602 39 50 773.1 0 50 40 16 773.1 0 16 41 16 773.1 0 16 42 13 773.1 0 13 43 199 773.1 0 199 44 383 773.1 0 383 45 944 773.1 0 944 46 6 773.1 0 6 47 11 773.1 0 11 48 12 773.1 0 12 49 21 773.1 0 21 50 18 773.1 0 18 51 8 773.1 0 8 52 20 773.1 0 20 53 12 773.1 0 12 54 13 773.1 0 13 55 28 773.1 0 28 56 19 773.1 0 19 57 48 773.1 0 48 58 14 773.1 0 14 59 13 773.1 0 13 60 7 773.1 0 7 61 18 773.1 0 18 62 13 773.1 0 13 63 23 773.1 0 23 64 17 773.1 0 17 65 12 773.1 0 12 66 10 773.1 0 10 67 14 773.1 0 14 68 13 773.1 0 13 69 23 773.1 0 23 70 11 773.1 0 11 71 26 773.1 0 26 72 42 773.1 0 42 73 25 773.1 0 25 74 26 773.1 0 26 75 16 773.1 0 16 76 12 773.1 0 12 77 23 773.1 0 23 78 20 773.1 0 20 79 40 773.1 0 40 80 26 773.1 0 26 81 20 773.1 0 20 82 33 773.1 0 33 83 33 773.1 0 33 84 19 773.1 0 19 85 27 773.1 0 27 86 27 773.1 0 27 87 23 773.1 0 23 88 23 773.1 0 23 89 22 773.1 0 22 90 21 773.1 0 21 91 12 773.1 0 12 92 11 773.1 0 11 93 14 773.1 0 14 94 19 773.1 0 19 95 23 773.1 0 23 96 37 773.1 0 37 97 36 773.1 0 36 98 22 773.1 0 22 99 13 773.1 0 13 100 6 773.1 0 6 101 21 773.1 0 21 102 17 773.1 0 17 103 12 773.1 0 12 104 21 773.1 0 21 105 20 773.1 0 20 106 21 773.1 0 21 107 26 773.1 0 26 108 28 773.1 0 28 109 16 773.1 0 16 110 13 773.1 0 13 111 18 773.1 0 18 112 27 773.1 0 27 113 33 773.1 0 33 114 35 773.1 0 35 115 33 773.1 0 33 116 23 773.1 0 23 117 7 773.1 0 7 118 25 773.1 0 25 119 23 773.1 0 23 120 26 773.1 0 26 121 27 773.1 0 27 122 66 773.1 0 66 123 22 773.1 0 22 124 19 773.1 0 19 125 33 773.1 0 33 126 122 773.1 0 122 127 125 773.1 0 125 128 190 773.1 0 190 129 76 773.1 0 76 130 80 773.1 0 80 131 130 773.1 0 130 132 129 773.1 0 129 133 251 773.1 0 251 134 50 773.1 0 50 135 339 773.1 0 339 136 141 773.1 0 141 137 297 773.1 0 297 138 34 773.1 0 34 139 60 773.1 0 60 140 124 773.1 0 124 141 165 773.1 0 165 142 165 773.1 0 165 143 53 773.1 0 53 144 30 773.1 0 30 145 47 773.1 0 47 146 44 773.1 0 44 147 64 773.1 0 64 148 157 773.1 0 157 149 235 773.1 0 235 150 180 773.1 0 180 151 35 773.1 0 35 152 35 773.1 0 35 153 24 773.1 0 24 154 34 773.1 0 34 155 43 773.1 0 43 156 23 773.1 0 23 157 28 773.1 0 28 158 16 773.1 0 16 159 38 773.1 0 38 160 78 773.1 0 78 161 46 773.1 0 46 162 36 773.1 0 36 163 35 773.1 0 35 164 25 773.1 0 25 165 36 773.1 0 36 166 54 773.1 0 54 167 67 773.1 0 67 168 37 773.1 0 37 169 89 773.1 0 89 170 25 773.1 0 25 171 19 773.1 0 19 172 34 773.1 0 34 173 27 773.1 0 27 174 27 773.1 0 27 175 45 773.1 0 45 176 32 773.1 0 32 177 35 773.1 0 35 178 41 773.1 0 41 179 34 773.1 0 34 180 42 773.1 0 42 181 46 773.1 0 46 182 85 773.1 0 85 183 54 773.1 0 54 184 58 773.1 0 58 185 52 773.1 0 52 186 44 773.1 0 44 187 47 773.1 0 47 188 35 773.1 0 35 189 140 773.1 0 140 190 45 773.1 0 45 191 54 773.1 0 54 192 35 773.1 0 35 193 31 773.1 0 31 194 69 773.1 0 69 195 44 773.1 0 44 196 53 773.1 0 53 197 18 773.1 0 18 198 28 773.1 0 28 199 22 773.1 0 22 200 33 773.1 0 33 201 51 773.1 0 51 202 47 773.1 0 47 203 28 773.1 0 28 204 24 773.1 0 24 205 28 773.1 0 28 206 30 773.1 0 30 207 95 773.1 0 95 208 168 773.1 0 168 209 239 773.1 0 239 210 99 773.1 0 99 211 66 773.1 0 66 212 46 773.1 0 46 213 113 773.1 0 113 214 183 773.1 0 183 215 196 773.1 0 196 216 118 773.1 0 118 217 62 773.1 0 62 218 36 773.1 0 36 219 60 773.1 0 60 220 63 773.1 0 63 221 80 773.1 0 80 222 52 773.1 0 52 223 40 773.1 0 40 224 38 773.1 0 38 225 52 773.1 0 52 226 54 773.1 0 54 227 48 773.1 0 48 228 75 773.1 0 75 229 67 773.1 0 67 230 116 773.1 0 116 231 59 773.1 0 59 232 59 773.1 0 59 233 103 773.1 0 103 234 112 773.1 0 112 235 120 773.1 0 120 236 77 773.1 0 77 237 36 773.1 0 36 238 31 773.1 0 31 239 51 773.1 0 51 240 44 773.1 0 44 241 41 773.1 0 41 242 59 773.1 0 59 243 60 773.1 0 60 244 73 773.1 0 73 245 56 773.1 0 56 246 46 773.1 0 46 247 39 773.1 0 39 248 43 773.1 0 43 249 43 773.1 0 43 250 39 773.1 0 39 251 39 773.1 0 39 252 33 773.1 0 33 253 47 773.1 0 47 254 55 773.1 0 55 255 44 773.1 0 44 256 56 773.1 0 56 257 61 773.1 0 61 258 67 773.1 0 67 259 74 773.1 0 74 260 79 773.1 0 79 261 78 773.1 0 78 262 61 773.1 0 61 263 47 773.1 0 47 264 53 773.1 0 53 265 56 773.1 0 56 266 50 773.1 0 50 267 59 773.1 0 59 268 99 773.1 0 99 269 243 773.1 0 243 270 144 773.1 0 144 271 277 773.1 0 277 272 183 773.1 0 183 273 170 773.1 0 170 274 229 773.1 0 229 275 133 773.1 0 133 276 94 773.1 0 94 277 91 773.1 0 91 278 44 773.1 0 44 279 129 773.1 0 129 280 171 773.1 0 171 281 350 773.1 0 350 282 142 773.1 0 142 283 96 773.1 0 96 284 66 773.1 0 66 285 75 773.1 0 75 286 78 773.1 0 78 287 60 773.1 0 60 288 83 773.1 0 83 289 90 773.1 0 90 290 90 773.1 0 90 291 87 773.1 0 87 292 88 773.1 0 88 293 166 773.1 0 166 294 498 773.1 0 498 295 839 773.1 0 839 296 765 773.1 0 765 297 463 773.1 0 463 298 165 773.1 0 165 299 82 773.1 0 82 300 160 773.1 0 160 301 376 773.1 0 376 Finished in 15.00 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 208,744 Reads with adapters: 105,802 (50.7%) Reads that were too short: 80 (0.0%) Reads written (passing filters): 105,722 (50.6%) Total basepairs processed: 60,240,832 bp Total written (filtered): 29,261,356 bp (48.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 105802 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 54 0.0 3 0 0 36 18 16 834 0.0 3 0 728 64 42 17 103958 0.0 3 98691 4378 718 171 18 241 0.0 3 26 177 12 26 19 213 0.0 3 3 0 119 91 20 243 0.0 3 1 1 0 241 21 4 0.0 3 1 0 1 2 25 1 0.0 3 0 1 26 1 0.0 3 0 0 1 27 1 0.0 3 1 29 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 34 1 0.0 3 1 40 1 0.0 3 0 0 0 1 43 8 0.0 3 0 0 0 8 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 19 0.0 3 0 0 0 19 53 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 65 14 0.0 3 1 0 0 13 66 3 0.0 3 0 0 1 2 68 2 0.0 3 0 0 0 2 73 1 0.0 3 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 1 0 0 1 78 1 0.0 3 0 0 0 1 79 5 0.0 3 0 0 1 4 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 2 0.0 3 1 0 0 1 84 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 16 0.0 3 0 0 0 16 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 3 0.0 3 0 0 0 3 135 9 0.0 3 0 0 0 9 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 148 6 0.0 3 0 0 0 6 149 6 0.0 3 0 0 0 6 150 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 4 0.0 3 0 0 0 4 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 185 4 0.0 3 0 0 0 4 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 205 6 0.0 3 0 0 0 6 210 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 1 1 282 11 0.0 3 0 0 0 11 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.84 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 105,722 Reads with adapters: 1,094 (1.0%) Reads written (passing filters): 105,722 (100.0%) Total basepairs processed: 29,261,356 bp Total written (filtered): 29,258,011 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1094 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.0% C: 27.4% G: 18.1% T: 29.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1047 1651.9 0 1047 4 34 413.0 0 34 5 10 103.2 0 10 6 3 25.8 0 3