This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 149.94 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 215,903 (6.8%) Reads that were too short: 5,902 (0.2%) Reads written (passing filters): 210,001 (6.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 60,880,754 bp (6.4%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 215903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1199 773.1 0 1199 7 95909 773.1 0 95909 8 105712 773.1 0 105712 9 70 773.1 0 70 10 12 773.1 0 12 11 8 773.1 0 8 12 9 773.1 0 9 13 9 773.1 0 9 14 11 773.1 0 11 15 18 773.1 0 18 16 17 773.1 0 17 17 16 773.1 0 16 18 26 773.1 0 26 19 21 773.1 0 21 20 16 773.1 0 16 21 17 773.1 0 17 22 18 773.1 0 18 23 30 773.1 0 30 24 19 773.1 0 19 25 26 773.1 0 26 26 14 773.1 0 14 27 26 773.1 0 26 28 12 773.1 0 12 29 15 773.1 0 15 30 20 773.1 0 20 31 15 773.1 0 15 32 10 773.1 0 10 33 15 773.1 0 15 34 14 773.1 0 14 35 17 773.1 0 17 36 132 773.1 0 132 37 128 773.1 0 128 38 218 773.1 0 218 39 24 773.1 0 24 40 16 773.1 0 16 41 28 773.1 0 28 42 17 773.1 0 17 43 52 773.1 0 52 44 122 773.1 0 122 45 234 773.1 0 234 46 13 773.1 0 13 47 14 773.1 0 14 48 17 773.1 0 17 49 27 773.1 0 27 50 23 773.1 0 23 51 9 773.1 0 9 52 26 773.1 0 26 53 20 773.1 0 20 54 14 773.1 0 14 55 22 773.1 0 22 56 37 773.1 0 37 57 23 773.1 0 23 58 27 773.1 0 27 59 31 773.1 0 31 60 14 773.1 0 14 61 23 773.1 0 23 62 16 773.1 0 16 63 23 773.1 0 23 64 9 773.1 0 9 65 14 773.1 0 14 66 6 773.1 0 6 67 9 773.1 0 9 68 9 773.1 0 9 69 15 773.1 0 15 70 7 773.1 0 7 71 10 773.1 0 10 72 47 773.1 0 47 73 16 773.1 0 16 74 16 773.1 0 16 75 23 773.1 0 23 76 26 773.1 0 26 77 22 773.1 0 22 78 29 773.1 0 29 79 22 773.1 0 22 80 15 773.1 0 15 81 13 773.1 0 13 82 26 773.1 0 26 83 15 773.1 0 15 84 28 773.1 0 28 85 17 773.1 0 17 86 19 773.1 0 19 87 35 773.1 0 35 88 25 773.1 0 25 89 28 773.1 0 28 90 24 773.1 0 24 91 20 773.1 0 20 92 30 773.1 0 30 93 24 773.1 0 24 94 29 773.1 0 29 95 26 773.1 0 26 96 50 773.1 0 50 97 23 773.1 0 23 98 19 773.1 0 19 99 18 773.1 0 18 100 17 773.1 0 17 101 12 773.1 0 12 102 16 773.1 0 16 103 16 773.1 0 16 104 27 773.1 0 27 105 16 773.1 0 16 106 18 773.1 0 18 107 9 773.1 0 9 108 31 773.1 0 31 109 16 773.1 0 16 110 9 773.1 0 9 111 14 773.1 0 14 112 15 773.1 0 15 113 16 773.1 0 16 114 24 773.1 0 24 115 33 773.1 0 33 116 17 773.1 0 17 117 13 773.1 0 13 118 25 773.1 0 25 119 14 773.1 0 14 120 30 773.1 0 30 121 28 773.1 0 28 122 43 773.1 0 43 123 28 773.1 0 28 124 16 773.1 0 16 125 24 773.1 0 24 126 71 773.1 0 71 127 88 773.1 0 88 128 72 773.1 0 72 129 27 773.1 0 27 130 50 773.1 0 50 131 191 773.1 0 191 132 149 773.1 0 149 133 275 773.1 0 275 134 78 773.1 0 78 135 324 773.1 0 324 136 147 773.1 0 147 137 216 773.1 0 216 138 32 773.1 0 32 139 58 773.1 0 58 140 94 773.1 0 94 141 130 773.1 0 130 142 138 773.1 0 138 143 38 773.1 0 38 144 18 773.1 0 18 145 28 773.1 0 28 146 28 773.1 0 28 147 54 773.1 0 54 148 88 773.1 0 88 149 120 773.1 0 120 150 108 773.1 0 108 151 35 773.1 0 35 152 30 773.1 0 30 153 24 773.1 0 24 154 24 773.1 0 24 155 22 773.1 0 22 156 14 773.1 0 14 157 18 773.1 0 18 158 19 773.1 0 19 159 21 773.1 0 21 160 54 773.1 0 54 161 26 773.1 0 26 162 17 773.1 0 17 163 23 773.1 0 23 164 19 773.1 0 19 165 17 773.1 0 17 166 21 773.1 0 21 167 25 773.1 0 25 168 15 773.1 0 15 169 71 773.1 0 71 170 13 773.1 0 13 171 25 773.1 0 25 172 21 773.1 0 21 173 26 773.1 0 26 174 26 773.1 0 26 175 16 773.1 0 16 176 29 773.1 0 29 177 22 773.1 0 22 178 15 773.1 0 15 179 22 773.1 0 22 180 33 773.1 0 33 181 28 773.1 0 28 182 24 773.1 0 24 183 23 773.1 0 23 184 31 773.1 0 31 185 29 773.1 0 29 186 27 773.1 0 27 187 28 773.1 0 28 188 28 773.1 0 28 189 135 773.1 0 135 190 21 773.1 0 21 191 26 773.1 0 26 192 25 773.1 0 25 193 20 773.1 0 20 194 52 773.1 0 52 195 39 773.1 0 39 196 29 773.1 0 29 197 12 773.1 0 12 198 22 773.1 0 22 199 31 773.1 0 31 200 17 773.1 0 17 201 27 773.1 0 27 202 39 773.1 0 39 203 28 773.1 0 28 204 27 773.1 0 27 205 21 773.1 0 21 206 13 773.1 0 13 207 53 773.1 0 53 208 59 773.1 0 59 209 78 773.1 0 78 210 44 773.1 0 44 211 45 773.1 0 45 212 34 773.1 0 34 213 39 773.1 0 39 214 74 773.1 0 74 215 79 773.1 0 79 216 62 773.1 0 62 217 35 773.1 0 35 218 17 773.1 0 17 219 39 773.1 0 39 220 32 773.1 0 32 221 35 773.1 0 35 222 32 773.1 0 32 223 24 773.1 0 24 224 19 773.1 0 19 225 29 773.1 0 29 226 35 773.1 0 35 227 24 773.1 0 24 228 33 773.1 0 33 229 40 773.1 0 40 230 44 773.1 0 44 231 29 773.1 0 29 232 27 773.1 0 27 233 44 773.1 0 44 234 45 773.1 0 45 235 34 773.1 0 34 236 47 773.1 0 47 237 21 773.1 0 21 238 30 773.1 0 30 239 29 773.1 0 29 240 22 773.1 0 22 241 19 773.1 0 19 242 41 773.1 0 41 243 30 773.1 0 30 244 32 773.1 0 32 245 24 773.1 0 24 246 27 773.1 0 27 247 21 773.1 0 21 248 19 773.1 0 19 249 25 773.1 0 25 250 12 773.1 0 12 251 27 773.1 0 27 252 23 773.1 0 23 253 36 773.1 0 36 254 42 773.1 0 42 255 35 773.1 0 35 256 25 773.1 0 25 257 24 773.1 0 24 258 35 773.1 0 35 259 29 773.1 0 29 260 39 773.1 0 39 261 44 773.1 0 44 262 33 773.1 0 33 263 32 773.1 0 32 264 35 773.1 0 35 265 23 773.1 0 23 266 31 773.1 0 31 267 32 773.1 0 32 268 42 773.1 0 42 269 125 773.1 0 125 270 91 773.1 0 91 271 142 773.1 0 142 272 64 773.1 0 64 273 52 773.1 0 52 274 89 773.1 0 89 275 67 773.1 0 67 276 54 773.1 0 54 277 38 773.1 0 38 278 31 773.1 0 31 279 65 773.1 0 65 280 105 773.1 0 105 281 191 773.1 0 191 282 53 773.1 0 53 283 41 773.1 0 41 284 32 773.1 0 32 285 46 773.1 0 46 286 46 773.1 0 46 287 43 773.1 0 43 288 44 773.1 0 44 289 35 773.1 0 35 290 42 773.1 0 42 291 73 773.1 0 73 292 51 773.1 0 51 293 67 773.1 0 67 294 274 773.1 0 274 295 531 773.1 0 531 296 432 773.1 0 432 297 223 773.1 0 223 298 81 773.1 0 81 299 38 773.1 0 38 300 95 773.1 0 95 301 243 773.1 0 243 Finished in 16.60 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 210,001 Reads with adapters: 108,796 (51.8%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 108,777 (51.8%) Total basepairs processed: 60,880,754 bp Total written (filtered): 29,648,746 bp (48.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 108796 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 28 0.0 3 0 0 26 2 19 996 0.0 3 0 961 26 6 3 20 106967 0.0 4 105264 1643 54 5 1 21 214 0.0 4 48 164 1 1 22 17 0.0 4 1 2 13 0 1 23 69 0.0 4 1 0 0 60 8 25 1 0.0 4 0 1 26 1 0.0 4 1 28 1 0.0 4 1 31 1 0.0 4 1 76 1 0.0 4 1 78 1 0.0 4 0 1 79 1 0.0 4 1 87 1 0.0 4 1 88 1 0.0 4 1 91 8 0.0 4 0 0 0 0 8 92 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 98 4 0.0 4 0 0 0 0 4 101 1 0.0 4 1 115 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 450 0.0 4 0 0 0 0 450 153 5 0.0 4 0 0 0 0 5 207 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 4 0.0 4 0 0 0 1 3 247 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.31 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 108,777 Reads with adapters: 7,323 (6.7%) Reads written (passing filters): 108,777 (100.0%) Total basepairs processed: 29,648,746 bp Total written (filtered): 29,331,165 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7323 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 5.0% G: 83.3% T: 6.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 50 1699.6 0 50 4 22 424.9 0 22 5 10 106.2 0 10 6 3 26.6 0 3 8 2 1.7 0 2 9 74 0.4 0 0 74 10 1947 0.1 1 66 1881 11 4227 0.0 1 25 4202 12 15 0.0 1 0 15 13 1 0.0 1 0 1 14 1 0.0 1 0 1 18 22 0.0 1 15 7 47 1 0.0 1 1 71 1 0.0 1 1 79 1 0.0 1 1 108 1 0.0 1 0 1 151 1 0.0 1 0 1 190 2 0.0 1 0 2 209 1 0.0 1 0 1 224 25 0.0 1 24 1 225 1 0.0 1 1 230 15 0.0 1 14 1 257 1 0.0 1 1 259 245 0.0 1 231 14 260 7 0.0 1 7 268 1 0.0 1 1 269 523 0.0 1 483 40 270 117 0.0 1 113 4 271 6 0.0 1 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.