This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.99 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 365,884 (11.6%) Reads that were too short: 11,936 (0.4%) Reads written (passing filters): 353,948 (11.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 101,259,026 bp (10.6%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 365884 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7931 773.1 0 7931 7 143502 773.1 0 143502 8 2715 773.1 0 2715 9 176893 773.1 0 176893 10 110 773.1 0 110 11 61 773.1 0 61 12 24 773.1 0 24 13 64 773.1 0 64 14 55 773.1 0 55 15 42 773.1 0 42 16 53 773.1 0 53 17 80 773.1 0 80 18 45 773.1 0 45 19 54 773.1 0 54 20 31 773.1 0 31 21 40 773.1 0 40 22 41 773.1 0 41 23 62 773.1 0 62 24 33 773.1 0 33 25 25 773.1 0 25 26 44 773.1 0 44 27 48 773.1 0 48 28 57 773.1 0 57 29 52 773.1 0 52 30 50 773.1 0 50 31 49 773.1 0 49 32 36 773.1 0 36 33 28 773.1 0 28 34 54 773.1 0 54 35 49 773.1 0 49 36 41 773.1 0 41 37 52 773.1 0 52 38 40 773.1 0 40 39 54 773.1 0 54 40 53 773.1 0 53 41 57 773.1 0 57 42 159 773.1 0 159 43 148 773.1 0 148 44 134 773.1 0 134 45 33 773.1 0 33 46 41 773.1 0 41 47 45 773.1 0 45 48 55 773.1 0 55 49 57 773.1 0 57 50 76 773.1 0 76 51 49 773.1 0 49 52 57 773.1 0 57 53 34 773.1 0 34 54 46 773.1 0 46 55 58 773.1 0 58 56 40 773.1 0 40 57 38 773.1 0 38 58 61 773.1 0 61 59 50 773.1 0 50 60 24 773.1 0 24 61 40 773.1 0 40 62 55 773.1 0 55 63 32 773.1 0 32 64 43 773.1 0 43 65 85 773.1 0 85 66 75 773.1 0 75 67 59 773.1 0 59 68 31 773.1 0 31 69 46 773.1 0 46 70 37 773.1 0 37 71 42 773.1 0 42 72 47 773.1 0 47 73 76 773.1 0 76 74 62 773.1 0 62 75 153 773.1 0 153 76 98 773.1 0 98 77 103 773.1 0 103 78 46 773.1 0 46 79 128 773.1 0 128 80 135 773.1 0 135 81 67 773.1 0 67 82 43 773.1 0 43 83 35 773.1 0 35 84 36 773.1 0 36 85 55 773.1 0 55 86 60 773.1 0 60 87 62 773.1 0 62 88 54 773.1 0 54 89 59 773.1 0 59 90 97 773.1 0 97 91 99 773.1 0 99 92 872 773.1 0 872 93 1404 773.1 0 1404 94 4347 773.1 0 4347 95 56 773.1 0 56 96 41 773.1 0 41 97 37 773.1 0 37 98 44 773.1 0 44 99 47 773.1 0 47 100 43 773.1 0 43 101 38 773.1 0 38 102 35 773.1 0 35 103 22 773.1 0 22 104 43 773.1 0 43 105 59 773.1 0 59 106 51 773.1 0 51 107 46 773.1 0 46 108 58 773.1 0 58 109 57 773.1 0 57 110 68 773.1 0 68 111 48 773.1 0 48 112 49 773.1 0 49 113 90 773.1 0 90 114 93 773.1 0 93 115 41 773.1 0 41 116 63 773.1 0 63 117 75 773.1 0 75 118 70 773.1 0 70 119 84 773.1 0 84 120 65 773.1 0 65 121 84 773.1 0 84 122 56 773.1 0 56 123 438 773.1 0 438 124 1337 773.1 0 1337 125 601 773.1 0 601 126 87 773.1 0 87 127 60 773.1 0 60 128 150 773.1 0 150 129 128 773.1 0 128 130 273 773.1 0 273 131 299 773.1 0 299 132 286 773.1 0 286 133 35 773.1 0 35 134 47 773.1 0 47 135 48 773.1 0 48 136 155 773.1 0 155 137 51 773.1 0 51 138 56 773.1 0 56 139 42 773.1 0 42 140 39 773.1 0 39 141 36 773.1 0 36 142 37 773.1 0 37 143 42 773.1 0 42 144 41 773.1 0 41 145 37 773.1 0 37 146 84 773.1 0 84 147 68 773.1 0 68 148 73 773.1 0 73 149 31 773.1 0 31 150 32 773.1 0 32 151 45 773.1 0 45 152 26 773.1 0 26 153 32 773.1 0 32 154 39 773.1 0 39 155 54 773.1 0 54 156 38 773.1 0 38 157 23 773.1 0 23 158 39 773.1 0 39 159 47 773.1 0 47 160 41 773.1 0 41 161 35 773.1 0 35 162 52 773.1 0 52 163 53 773.1 0 53 164 49 773.1 0 49 165 39 773.1 0 39 166 49 773.1 0 49 167 96 773.1 0 96 168 63 773.1 0 63 169 61 773.1 0 61 170 33 773.1 0 33 171 47 773.1 0 47 172 54 773.1 0 54 173 140 773.1 0 140 174 75 773.1 0 75 175 70 773.1 0 70 176 86 773.1 0 86 177 60 773.1 0 60 178 235 773.1 0 235 179 305 773.1 0 305 180 387 773.1 0 387 181 86 773.1 0 86 182 44 773.1 0 44 183 60 773.1 0 60 184 54 773.1 0 54 185 129 773.1 0 129 186 83 773.1 0 83 187 44 773.1 0 44 188 79 773.1 0 79 189 62 773.1 0 62 190 49 773.1 0 49 191 39 773.1 0 39 192 66 773.1 0 66 193 94 773.1 0 94 194 111 773.1 0 111 195 143 773.1 0 143 196 127 773.1 0 127 197 91 773.1 0 91 198 334 773.1 0 334 199 402 773.1 0 402 200 397 773.1 0 397 201 392 773.1 0 392 202 125 773.1 0 125 203 101 773.1 0 101 204 63 773.1 0 63 205 103 773.1 0 103 206 109 773.1 0 109 207 136 773.1 0 136 208 90 773.1 0 90 209 73 773.1 0 73 210 60 773.1 0 60 211 57 773.1 0 57 212 47 773.1 0 47 213 67 773.1 0 67 214 46 773.1 0 46 215 90 773.1 0 90 216 77 773.1 0 77 217 84 773.1 0 84 218 100 773.1 0 100 219 284 773.1 0 284 220 228 773.1 0 228 221 249 773.1 0 249 222 351 773.1 0 351 223 208 773.1 0 208 224 320 773.1 0 320 225 81 773.1 0 81 226 77 773.1 0 77 227 68 773.1 0 68 228 136 773.1 0 136 229 69 773.1 0 69 230 71 773.1 0 71 231 37 773.1 0 37 232 54 773.1 0 54 233 36 773.1 0 36 234 48 773.1 0 48 235 48 773.1 0 48 236 73 773.1 0 73 237 44 773.1 0 44 238 210 773.1 0 210 239 242 773.1 0 242 240 586 773.1 0 586 241 547 773.1 0 547 242 911 773.1 0 911 243 62 773.1 0 62 244 59 773.1 0 59 245 71 773.1 0 71 246 66 773.1 0 66 247 89 773.1 0 89 248 117 773.1 0 117 249 73 773.1 0 73 250 92 773.1 0 92 251 162 773.1 0 162 252 118 773.1 0 118 253 92 773.1 0 92 254 75 773.1 0 75 255 77 773.1 0 77 256 61 773.1 0 61 257 70 773.1 0 70 258 50 773.1 0 50 259 44 773.1 0 44 260 63 773.1 0 63 261 114 773.1 0 114 262 63 773.1 0 63 263 85 773.1 0 85 264 66 773.1 0 66 265 51 773.1 0 51 266 52 773.1 0 52 267 57 773.1 0 57 268 43 773.1 0 43 269 53 773.1 0 53 270 67 773.1 0 67 271 57 773.1 0 57 272 66 773.1 0 66 273 46 773.1 0 46 274 40 773.1 0 40 275 53 773.1 0 53 276 43 773.1 0 43 277 80 773.1 0 80 278 88 773.1 0 88 279 99 773.1 0 99 280 53 773.1 0 53 281 57 773.1 0 57 282 53 773.1 0 53 283 94 773.1 0 94 284 121 773.1 0 121 285 89 773.1 0 89 286 124 773.1 0 124 287 107 773.1 0 107 288 366 773.1 0 366 289 325 773.1 0 325 290 403 773.1 0 403 291 171 773.1 0 171 292 110 773.1 0 110 293 60 773.1 0 60 294 54 773.1 0 54 295 60 773.1 0 60 296 71 773.1 0 71 297 77 773.1 0 77 298 63 773.1 0 63 299 153 773.1 0 153 300 161 773.1 0 161 301 94 773.1 0 94 Finished in 25.10 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 353,948 Reads with adapters: 181,094 (51.2%) Reads that were too short: 118 (0.0%) Reads written (passing filters): 180,976 (51.1%) Total basepairs processed: 101,259,026 bp Total written (filtered): 49,758,411 bp (49.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 181094 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 34 0.0 2 0 0 0 34 15 91 0.0 3 0 0 68 23 16 1292 0.0 3 0 1085 136 71 17 178090 0.0 3 168285 8043 1418 344 18 444 0.0 3 29 268 29 118 19 353 0.0 3 0 6 211 136 20 575 0.0 3 291 18 2 264 22 1 0.0 3 0 0 1 23 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 36 1 0.0 3 1 38 1 0.0 3 1 44 3 0.0 3 0 0 1 2 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 63 5 0.0 3 0 0 0 5 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 112 5 0.0 3 0 0 0 5 113 5 0.0 3 0 0 0 5 114 2 0.0 3 0 0 0 2 115 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 1 132 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 5 0.0 3 0 0 0 5 157 3 0.0 3 0 0 0 3 159 4 0.0 3 0 0 0 4 164 9 0.0 3 0 0 0 9 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 4 0.0 3 0 0 1 3 175 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 5 0.0 3 0 0 0 5 193 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 1 5 200 2 0.0 3 0 0 1 1 202 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 1 219 1 0.0 3 0 0 0 1 221 7 0.0 3 0 0 0 7 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 8 0.0 3 0 0 0 8 281 12 0.0 3 0 0 0 12 282 6 0.0 3 0 0 1 5 283 2 0.0 3 0 0 0 2 285 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 10.09 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 180,976 Reads with adapters: 6,157 (3.4%) Reads written (passing filters): 180,976 (100.0%) Total basepairs processed: 49,758,411 bp Total written (filtered): 49,739,801 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6157 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.4% C: 10.9% G: 21.8% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6036 2827.8 0 6036 4 113 706.9 0 113 5 5 176.7 0 5 7 1 11.0 0 1 9 2 0.7 0 0 2