This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 147.49 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 368,316 (11.6%) Reads that were too short: 8,161 (0.3%) Reads written (passing filters): 360,155 (11.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 103,725,337 bp (10.9%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 368316 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7444 773.1 0 7444 7 181049 773.1 0 181049 8 1663 773.1 0 1663 9 152009 773.1 0 152009 10 84 773.1 0 84 11 24 773.1 0 24 12 23 773.1 0 23 13 35 773.1 0 35 14 27 773.1 0 27 15 30 773.1 0 30 16 34 773.1 0 34 17 30 773.1 0 30 18 24 773.1 0 24 19 32 773.1 0 32 20 17 773.1 0 17 21 26 773.1 0 26 22 29 773.1 0 29 23 38 773.1 0 38 24 23 773.1 0 23 25 16 773.1 0 16 26 30 773.1 0 30 27 29 773.1 0 29 28 33 773.1 0 33 29 33 773.1 0 33 30 28 773.1 0 28 31 37 773.1 0 37 32 30 773.1 0 30 33 25 773.1 0 25 34 26 773.1 0 26 35 37 773.1 0 37 36 18 773.1 0 18 37 27 773.1 0 27 38 29 773.1 0 29 39 30 773.1 0 30 40 32 773.1 0 32 41 46 773.1 0 46 42 78 773.1 0 78 43 94 773.1 0 94 44 69 773.1 0 69 45 26 773.1 0 26 46 16 773.1 0 16 47 20 773.1 0 20 48 27 773.1 0 27 49 25 773.1 0 25 50 40 773.1 0 40 51 27 773.1 0 27 52 25 773.1 0 25 53 13 773.1 0 13 54 34 773.1 0 34 55 40 773.1 0 40 56 35 773.1 0 35 57 27 773.1 0 27 58 43 773.1 0 43 59 27 773.1 0 27 60 12 773.1 0 12 61 29 773.1 0 29 62 39 773.1 0 39 63 29 773.1 0 29 64 29 773.1 0 29 65 51 773.1 0 51 66 52 773.1 0 52 67 30 773.1 0 30 68 33 773.1 0 33 69 27 773.1 0 27 70 31 773.1 0 31 71 29 773.1 0 29 72 28 773.1 0 28 73 37 773.1 0 37 74 58 773.1 0 58 75 71 773.1 0 71 76 56 773.1 0 56 77 59 773.1 0 59 78 21 773.1 0 21 79 93 773.1 0 93 80 73 773.1 0 73 81 48 773.1 0 48 82 15 773.1 0 15 83 26 773.1 0 26 84 17 773.1 0 17 85 31 773.1 0 31 86 30 773.1 0 30 87 41 773.1 0 41 88 47 773.1 0 47 89 56 773.1 0 56 90 84 773.1 0 84 91 77 773.1 0 77 92 781 773.1 0 781 93 1533 773.1 0 1533 94 4613 773.1 0 4613 95 52 773.1 0 52 96 32 773.1 0 32 97 36 773.1 0 36 98 34 773.1 0 34 99 32 773.1 0 32 100 19 773.1 0 19 101 32 773.1 0 32 102 29 773.1 0 29 103 19 773.1 0 19 104 27 773.1 0 27 105 35 773.1 0 35 106 29 773.1 0 29 107 38 773.1 0 38 108 56 773.1 0 56 109 36 773.1 0 36 110 46 773.1 0 46 111 37 773.1 0 37 112 38 773.1 0 38 113 73 773.1 0 73 114 77 773.1 0 77 115 35 773.1 0 35 116 36 773.1 0 36 117 47 773.1 0 47 118 43 773.1 0 43 119 46 773.1 0 46 120 54 773.1 0 54 121 49 773.1 0 49 122 46 773.1 0 46 123 378 773.1 0 378 124 1326 773.1 0 1326 125 599 773.1 0 599 126 37 773.1 0 37 127 26 773.1 0 26 128 104 773.1 0 104 129 88 773.1 0 88 130 144 773.1 0 144 131 179 773.1 0 179 132 161 773.1 0 161 133 37 773.1 0 37 134 23 773.1 0 23 135 41 773.1 0 41 136 125 773.1 0 125 137 47 773.1 0 47 138 38 773.1 0 38 139 40 773.1 0 40 140 29 773.1 0 29 141 24 773.1 0 24 142 40 773.1 0 40 143 35 773.1 0 35 144 31 773.1 0 31 145 44 773.1 0 44 146 94 773.1 0 94 147 94 773.1 0 94 148 59 773.1 0 59 149 29 773.1 0 29 150 33 773.1 0 33 151 23 773.1 0 23 152 36 773.1 0 36 153 30 773.1 0 30 154 22 773.1 0 22 155 37 773.1 0 37 156 26 773.1 0 26 157 31 773.1 0 31 158 28 773.1 0 28 159 37 773.1 0 37 160 24 773.1 0 24 161 27 773.1 0 27 162 45 773.1 0 45 163 35 773.1 0 35 164 46 773.1 0 46 165 32 773.1 0 32 166 34 773.1 0 34 167 58 773.1 0 58 168 29 773.1 0 29 169 35 773.1 0 35 170 31 773.1 0 31 171 20 773.1 0 20 172 33 773.1 0 33 173 75 773.1 0 75 174 48 773.1 0 48 175 51 773.1 0 51 176 52 773.1 0 52 177 43 773.1 0 43 178 131 773.1 0 131 179 120 773.1 0 120 180 183 773.1 0 183 181 72 773.1 0 72 182 40 773.1 0 40 183 36 773.1 0 36 184 32 773.1 0 32 185 101 773.1 0 101 186 115 773.1 0 115 187 28 773.1 0 28 188 99 773.1 0 99 189 47 773.1 0 47 190 37 773.1 0 37 191 31 773.1 0 31 192 47 773.1 0 47 193 56 773.1 0 56 194 50 773.1 0 50 195 57 773.1 0 57 196 34 773.1 0 34 197 35 773.1 0 35 198 160 773.1 0 160 199 222 773.1 0 222 200 169 773.1 0 169 201 128 773.1 0 128 202 57 773.1 0 57 203 40 773.1 0 40 204 46 773.1 0 46 205 61 773.1 0 61 206 63 773.1 0 63 207 60 773.1 0 60 208 57 773.1 0 57 209 35 773.1 0 35 210 33 773.1 0 33 211 33 773.1 0 33 212 30 773.1 0 30 213 48 773.1 0 48 214 41 773.1 0 41 215 42 773.1 0 42 216 43 773.1 0 43 217 40 773.1 0 40 218 58 773.1 0 58 219 95 773.1 0 95 220 89 773.1 0 89 221 91 773.1 0 91 222 280 773.1 0 280 223 242 773.1 0 242 224 410 773.1 0 410 225 63 773.1 0 63 226 43 773.1 0 43 227 38 773.1 0 38 228 60 773.1 0 60 229 59 773.1 0 59 230 38 773.1 0 38 231 28 773.1 0 28 232 39 773.1 0 39 233 32 773.1 0 32 234 53 773.1 0 53 235 36 773.1 0 36 236 49 773.1 0 49 237 44 773.1 0 44 238 117 773.1 0 117 239 140 773.1 0 140 240 501 773.1 0 501 241 574 773.1 0 574 242 855 773.1 0 855 243 53 773.1 0 53 244 56 773.1 0 56 245 77 773.1 0 77 246 45 773.1 0 45 247 36 773.1 0 36 248 66 773.1 0 66 249 54 773.1 0 54 250 87 773.1 0 87 251 81 773.1 0 81 252 62 773.1 0 62 253 52 773.1 0 52 254 45 773.1 0 45 255 34 773.1 0 34 256 55 773.1 0 55 257 49 773.1 0 49 258 47 773.1 0 47 259 47 773.1 0 47 260 45 773.1 0 45 261 92 773.1 0 92 262 42 773.1 0 42 263 77 773.1 0 77 264 44 773.1 0 44 265 43 773.1 0 43 266 41 773.1 0 41 267 46 773.1 0 46 268 48 773.1 0 48 269 44 773.1 0 44 270 36 773.1 0 36 271 44 773.1 0 44 272 32 773.1 0 32 273 30 773.1 0 30 274 33 773.1 0 33 275 37 773.1 0 37 276 35 773.1 0 35 277 47 773.1 0 47 278 42 773.1 0 42 279 36 773.1 0 36 280 35 773.1 0 35 281 37 773.1 0 37 282 41 773.1 0 41 283 48 773.1 0 48 284 86 773.1 0 86 285 57 773.1 0 57 286 37 773.1 0 37 287 63 773.1 0 63 288 138 773.1 0 138 289 150 773.1 0 150 290 161 773.1 0 161 291 84 773.1 0 84 292 47 773.1 0 47 293 56 773.1 0 56 294 41 773.1 0 41 295 45 773.1 0 45 296 38 773.1 0 38 297 53 773.1 0 53 298 42 773.1 0 42 299 102 773.1 0 102 300 106 773.1 0 106 301 91 773.1 0 91 Finished in 26.67 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 360,155 Reads with adapters: 186,982 (51.9%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 186,937 (51.9%) Total basepairs processed: 103,725,337 bp Total written (filtered): 51,191,353 bp (49.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 186982 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 49 0.0 3 0 0 49 19 1710 0.0 3 0 1657 45 8 20 184396 0.0 4 181318 2975 98 4 1 21 318 0.0 4 36 279 3 22 23 0.0 4 1 0 21 0 1 23 109 0.0 4 2 0 2 94 11 24 2 0.0 4 0 0 0 1 1 30 1 0.0 4 1 31 1 0.0 4 0 1 33 1 0.0 4 1 35 1 0.0 4 1 37 1 0.0 4 1 46 1 0.0 4 1 68 1 0.0 4 1 85 1 0.0 4 0 0 0 0 1 86 20 0.0 4 0 0 0 0 20 92 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 266 0.0 4 0 0 0 0 266 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 21 0.0 4 0 0 0 0 21 139 1 0.0 4 1 144 1 0.0 4 1 153 5 0.0 4 0 0 0 1 4 174 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 217 1 0.0 4 1 228 2 0.0 4 0 0 0 0 2 229 5 0.0 4 0 0 0 1 4 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 262 3 0.0 4 0 0 0 0 3 263 2 0.0 4 0 1 0 0 1 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 285 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 10.22 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 186,937 Reads with adapters: 6,682 (3.6%) Reads written (passing filters): 186,937 (100.0%) Total basepairs processed: 51,191,353 bp Total written (filtered): 51,053,258 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6682 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.1% C: 2.8% G: 88.4% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 167 2920.9 0 167 4 22 730.2 0 22 5 12 182.6 0 12 6 3 45.6 0 3 7 1 11.4 0 1 8 1 2.9 0 1 9 19 0.7 0 3 16 10 57 0.2 1 6 51 11 5928 0.0 1 28 5900 12 86 0.0 1 5 81 13 16 0.0 1 5 11 14 4 0.0 1 1 3 15 21 0.0 1 13 8 16 1 0.0 1 0 1 19 1 0.0 1 1 23 1 0.0 1 1 25 1 0.0 1 0 1 56 1 0.0 1 1 71 1 0.0 1 1 72 1 0.0 1 1 80 1 0.0 1 1 93 1 0.0 1 0 1 99 1 0.0 1 1 119 1 0.0 1 0 1 152 1 0.0 1 0 1 159 1 0.0 1 1 172 1 0.0 1 1 179 1 0.0 1 1 203 3 0.0 1 2 1 204 1 0.0 1 1 206 262 0.0 1 245 17 207 19 0.0 1 19 210 21 0.0 1 10 11 211 1 0.0 1 1 225 21 0.0 1 20 1 226 1 0.0 1 0 1 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.