This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.02 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 149,773 (4.7%) Reads that were too short: 43,675 (1.4%) Reads written (passing filters): 106,098 (3.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 24,907,337 bp (2.6%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 149773 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 514 773.1 0 514 7 2256 773.1 0 2256 8 457 773.1 0 457 9 1810 773.1 0 1810 10 1327 773.1 0 1327 11 45468 773.1 0 45468 12 116 773.1 0 116 13 63 773.1 0 63 14 77 773.1 0 77 15 69 773.1 0 69 16 51 773.1 0 51 17 48 773.1 0 48 18 76 773.1 0 76 19 62 773.1 0 62 20 87 773.1 0 87 21 46 773.1 0 46 22 67 773.1 0 67 23 57 773.1 0 57 24 66 773.1 0 66 25 73 773.1 0 73 26 103 773.1 0 103 27 128 773.1 0 128 28 164 773.1 0 164 29 514 773.1 0 514 30 80 773.1 0 80 31 79 773.1 0 79 32 81 773.1 0 81 33 74 773.1 0 74 34 79 773.1 0 79 35 59 773.1 0 59 36 71 773.1 0 71 37 79 773.1 0 79 38 75 773.1 0 75 39 87 773.1 0 87 40 94 773.1 0 94 41 68 773.1 0 68 42 58 773.1 0 58 43 68 773.1 0 68 44 78 773.1 0 78 45 72 773.1 0 72 46 70 773.1 0 70 47 78 773.1 0 78 48 101 773.1 0 101 49 144 773.1 0 144 50 87 773.1 0 87 51 52 773.1 0 52 52 69 773.1 0 69 53 73 773.1 0 73 54 72 773.1 0 72 55 134 773.1 0 134 56 110 773.1 0 110 57 93 773.1 0 93 58 69 773.1 0 69 59 60 773.1 0 60 60 97 773.1 0 97 61 77 773.1 0 77 62 74 773.1 0 74 63 79 773.1 0 79 64 84 773.1 0 84 65 80 773.1 0 80 66 78 773.1 0 78 67 79 773.1 0 79 68 59 773.1 0 59 69 87 773.1 0 87 70 127 773.1 0 127 71 314 773.1 0 314 72 114 773.1 0 114 73 65 773.1 0 65 74 53 773.1 0 53 75 74 773.1 0 74 76 81 773.1 0 81 77 66 773.1 0 66 78 93 773.1 0 93 79 318 773.1 0 318 80 143 773.1 0 143 81 239 773.1 0 239 82 177 773.1 0 177 83 888 773.1 0 888 84 902 773.1 0 902 85 1060 773.1 0 1060 86 78 773.1 0 78 87 71 773.1 0 71 88 71 773.1 0 71 89 78 773.1 0 78 90 173 773.1 0 173 91 67 773.1 0 67 92 48 773.1 0 48 93 53 773.1 0 53 94 57 773.1 0 57 95 75 773.1 0 75 96 136 773.1 0 136 97 124 773.1 0 124 98 257 773.1 0 257 99 155 773.1 0 155 100 167 773.1 0 167 101 104 773.1 0 104 102 70 773.1 0 70 103 94 773.1 0 94 104 90 773.1 0 90 105 203 773.1 0 203 106 126 773.1 0 126 107 120 773.1 0 120 108 116 773.1 0 116 109 123 773.1 0 123 110 156 773.1 0 156 111 196 773.1 0 196 112 357 773.1 0 357 113 5353 773.1 0 5353 114 3227 773.1 0 3227 115 6507 773.1 0 6507 116 196 773.1 0 196 117 85 773.1 0 85 118 87 773.1 0 87 119 127 773.1 0 127 120 66 773.1 0 66 121 76 773.1 0 76 122 119 773.1 0 119 123 120 773.1 0 120 124 155 773.1 0 155 125 156 773.1 0 156 126 130 773.1 0 130 127 168 773.1 0 168 128 226 773.1 0 226 129 2260 773.1 0 2260 130 2782 773.1 0 2782 131 3775 773.1 0 3775 132 95 773.1 0 95 133 89 773.1 0 89 134 99 773.1 0 99 135 129 773.1 0 129 136 162 773.1 0 162 137 177 773.1 0 177 138 148 773.1 0 148 139 257 773.1 0 257 140 216 773.1 0 216 141 227 773.1 0 227 142 123 773.1 0 123 143 104 773.1 0 104 144 110 773.1 0 110 145 95 773.1 0 95 146 118 773.1 0 118 147 102 773.1 0 102 148 81 773.1 0 81 149 147 773.1 0 147 150 108 773.1 0 108 151 97 773.1 0 97 152 102 773.1 0 102 153 138 773.1 0 138 154 1053 773.1 0 1053 155 1346 773.1 0 1346 156 3245 773.1 0 3245 157 2130 773.1 0 2130 158 812 773.1 0 812 159 147 773.1 0 147 160 60 773.1 0 60 161 67 773.1 0 67 162 54 773.1 0 54 163 81 773.1 0 81 164 113 773.1 0 113 165 51 773.1 0 51 166 66 773.1 0 66 167 167 773.1 0 167 168 166 773.1 0 166 169 198 773.1 0 198 170 107 773.1 0 107 171 61 773.1 0 61 172 93 773.1 0 93 173 49 773.1 0 49 174 56 773.1 0 56 175 69 773.1 0 69 176 67 773.1 0 67 177 57 773.1 0 57 178 341 773.1 0 341 179 54 773.1 0 54 180 73 773.1 0 73 181 39 773.1 0 39 182 56 773.1 0 56 183 59 773.1 0 59 184 90 773.1 0 90 185 99 773.1 0 99 186 92 773.1 0 92 187 72 773.1 0 72 188 128 773.1 0 128 189 69 773.1 0 69 190 94 773.1 0 94 191 85 773.1 0 85 192 98 773.1 0 98 193 60 773.1 0 60 194 57 773.1 0 57 195 51 773.1 0 51 196 119 773.1 0 119 197 75 773.1 0 75 198 58 773.1 0 58 199 58 773.1 0 58 200 200 773.1 0 200 201 131 773.1 0 131 202 213 773.1 0 213 203 67 773.1 0 67 204 92 773.1 0 92 205 72 773.1 0 72 206 66 773.1 0 66 207 73 773.1 0 73 208 55 773.1 0 55 209 78 773.1 0 78 210 89 773.1 0 89 211 209 773.1 0 209 212 260 773.1 0 260 213 311 773.1 0 311 214 161 773.1 0 161 215 149 773.1 0 149 216 148 773.1 0 148 217 76 773.1 0 76 218 78 773.1 0 78 219 54 773.1 0 54 220 65 773.1 0 65 221 61 773.1 0 61 222 88 773.1 0 88 223 74 773.1 0 74 224 92 773.1 0 92 225 73 773.1 0 73 226 78 773.1 0 78 227 109 773.1 0 109 228 813 773.1 0 813 229 599 773.1 0 599 230 1114 773.1 0 1114 231 171 773.1 0 171 232 139 773.1 0 139 233 117 773.1 0 117 234 137 773.1 0 137 235 213 773.1 0 213 236 254 773.1 0 254 237 200 773.1 0 200 238 110 773.1 0 110 239 96 773.1 0 96 240 75 773.1 0 75 241 80 773.1 0 80 242 123 773.1 0 123 243 135 773.1 0 135 244 130 773.1 0 130 245 97 773.1 0 97 246 153 773.1 0 153 247 203 773.1 0 203 248 244 773.1 0 244 249 447 773.1 0 447 250 116 773.1 0 116 251 116 773.1 0 116 252 100 773.1 0 100 253 126 773.1 0 126 254 139 773.1 0 139 255 269 773.1 0 269 256 240 773.1 0 240 257 374 773.1 0 374 258 155 773.1 0 155 259 67 773.1 0 67 260 56 773.1 0 56 261 62 773.1 0 62 262 82 773.1 0 82 263 108 773.1 0 108 264 77 773.1 0 77 265 74 773.1 0 74 266 67 773.1 0 67 267 75 773.1 0 75 268 166 773.1 0 166 269 346 773.1 0 346 270 378 773.1 0 378 271 219 773.1 0 219 272 125 773.1 0 125 273 107 773.1 0 107 274 151 773.1 0 151 275 95 773.1 0 95 276 130 773.1 0 130 277 119 773.1 0 119 278 93 773.1 0 93 279 84 773.1 0 84 280 125 773.1 0 125 281 194 773.1 0 194 282 158 773.1 0 158 283 242 773.1 0 242 284 201 773.1 0 201 285 289 773.1 0 289 286 146 773.1 0 146 287 195 773.1 0 195 288 250 773.1 0 250 289 204 773.1 0 204 290 172 773.1 0 172 291 223 773.1 0 223 292 183 773.1 0 183 293 214 773.1 0 214 294 759 773.1 0 759 295 1558 773.1 0 1558 296 4302 773.1 0 4302 297 2606 773.1 0 2606 298 5046 773.1 0 5046 299 6986 773.1 0 6986 300 6477 773.1 0 6477 301 288 773.1 0 288 Finished in 6.31 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 106,098 Reads with adapters: 2,340 (2.2%) Reads that were too short: 187 (0.2%) Reads written (passing filters): 2,153 (2.0%) Total basepairs processed: 24,907,337 bp Total written (filtered): 526,712 bp (2.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2340 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 19 0.0 3 0 19 17 1660 0.0 3 1562 81 14 3 18 10 0.0 3 0 3 0 7 19 13 0.0 3 6 2 3 2 20 2 0.0 3 0 0 1 1 21 2 0.0 3 0 0 1 1 23 12 0.0 3 1 0 0 11 24 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 32 255 0.0 3 0 0 4 251 33 1 0.0 3 0 0 0 1 34 9 0.0 3 0 0 0 9 35 37 0.0 3 0 0 1 36 36 2 0.0 3 0 1 0 1 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 2 0.0 3 1 0 0 1 46 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 6 0.0 3 0 0 0 6 52 2 0.0 3 0 0 0 2 53 12 0.0 3 0 0 0 12 54 2 0.0 3 0 0 0 2 56 3 0.0 3 0 0 0 3 58 12 0.0 3 0 0 1 11 59 5 0.0 3 0 0 0 5 61 3 0.0 3 0 0 1 2 62 15 0.0 3 0 0 1 14 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 73 9 0.0 3 0 0 8 1 76 1 0.0 3 0 0 0 1 77 6 0.0 3 0 0 0 6 78 5 0.0 3 0 0 0 5 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 7 0.0 3 0 0 0 7 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 90 1 0.0 3 1 94 3 0.0 3 0 0 0 3 99 3 0.0 3 0 0 0 3 104 7 0.0 3 0 0 0 7 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 122 9 0.0 3 0 0 1 8 123 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 6 0.0 3 0 0 0 6 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 147 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 5 0.0 3 0 0 0 5 154 5 0.0 3 0 0 0 5 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 7 0.0 3 0 0 0 7 164 13 0.0 3 0 0 0 13 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 1 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 5 0.0 3 0 0 1 4 177 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 190 4 0.0 3 0 0 0 4 191 2 0.0 3 0 0 0 2 192 6 0.0 3 0 0 1 5 195 1 0.0 3 0 0 0 1 197 1 0.0 3 1 199 2 0.0 3 0 0 0 2 200 21 0.0 3 0 0 0 21 203 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 241 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 3 0.0 3 0 0 0 3 278 2 0.0 3 0 0 0 2 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 2,153 Reads with adapters: 14 (0.7%) Reads written (passing filters): 2,153 (100.0%) Total basepairs processed: 526,712 bp Total written (filtered): 526,666 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 14 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.1% C: 21.4% G: 21.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 33.6 0 11 4 2 8.4 0 2 5 1 2.1 0 1