This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 147.05 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 148,932 (4.7%) Reads that were too short: 35,946 (1.1%) Reads written (passing filters): 112,986 (3.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 26,926,971 bp (2.8%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 148932 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 486 773.1 0 486 7 2037 773.1 0 2037 8 434 773.1 0 434 9 1957 773.1 0 1957 10 1080 773.1 0 1080 11 53925 773.1 0 53925 12 115 773.1 0 115 13 54 773.1 0 54 14 52 773.1 0 52 15 65 773.1 0 65 16 55 773.1 0 55 17 40 773.1 0 40 18 71 773.1 0 71 19 47 773.1 0 47 20 60 773.1 0 60 21 61 773.1 0 61 22 67 773.1 0 67 23 54 773.1 0 54 24 51 773.1 0 51 25 60 773.1 0 60 26 56 773.1 0 56 27 63 773.1 0 63 28 81 773.1 0 81 29 499 773.1 0 499 30 79 773.1 0 79 31 65 773.1 0 65 32 84 773.1 0 84 33 70 773.1 0 70 34 86 773.1 0 86 35 69 773.1 0 69 36 76 773.1 0 76 37 78 773.1 0 78 38 75 773.1 0 75 39 77 773.1 0 77 40 81 773.1 0 81 41 63 773.1 0 63 42 72 773.1 0 72 43 43 773.1 0 43 44 71 773.1 0 71 45 63 773.1 0 63 46 58 773.1 0 58 47 57 773.1 0 57 48 87 773.1 0 87 49 136 773.1 0 136 50 56 773.1 0 56 51 76 773.1 0 76 52 57 773.1 0 57 53 56 773.1 0 56 54 75 773.1 0 75 55 101 773.1 0 101 56 101 773.1 0 101 57 63 773.1 0 63 58 57 773.1 0 57 59 85 773.1 0 85 60 86 773.1 0 86 61 76 773.1 0 76 62 79 773.1 0 79 63 66 773.1 0 66 64 78 773.1 0 78 65 60 773.1 0 60 66 82 773.1 0 82 67 62 773.1 0 62 68 75 773.1 0 75 69 90 773.1 0 90 70 132 773.1 0 132 71 298 773.1 0 298 72 83 773.1 0 83 73 71 773.1 0 71 74 48 773.1 0 48 75 57 773.1 0 57 76 68 773.1 0 68 77 71 773.1 0 71 78 82 773.1 0 82 79 234 773.1 0 234 80 106 773.1 0 106 81 249 773.1 0 249 82 186 773.1 0 186 83 1055 773.1 0 1055 84 1040 773.1 0 1040 85 1109 773.1 0 1109 86 59 773.1 0 59 87 78 773.1 0 78 88 67 773.1 0 67 89 72 773.1 0 72 90 154 773.1 0 154 91 65 773.1 0 65 92 56 773.1 0 56 93 65 773.1 0 65 94 65 773.1 0 65 95 69 773.1 0 69 96 158 773.1 0 158 97 93 773.1 0 93 98 262 773.1 0 262 99 168 773.1 0 168 100 164 773.1 0 164 101 124 773.1 0 124 102 71 773.1 0 71 103 121 773.1 0 121 104 69 773.1 0 69 105 226 773.1 0 226 106 115 773.1 0 115 107 140 773.1 0 140 108 112 773.1 0 112 109 121 773.1 0 121 110 174 773.1 0 174 111 186 773.1 0 186 112 285 773.1 0 285 113 4821 773.1 0 4821 114 2787 773.1 0 2787 115 5929 773.1 0 5929 116 207 773.1 0 207 117 73 773.1 0 73 118 98 773.1 0 98 119 130 773.1 0 130 120 90 773.1 0 90 121 74 773.1 0 74 122 119 773.1 0 119 123 99 773.1 0 99 124 122 773.1 0 122 125 151 773.1 0 151 126 126 773.1 0 126 127 124 773.1 0 124 128 159 773.1 0 159 129 2047 773.1 0 2047 130 2586 773.1 0 2586 131 3739 773.1 0 3739 132 98 773.1 0 98 133 73 773.1 0 73 134 93 773.1 0 93 135 91 773.1 0 91 136 102 773.1 0 102 137 95 773.1 0 95 138 130 773.1 0 130 139 124 773.1 0 124 140 118 773.1 0 118 141 133 773.1 0 133 142 97 773.1 0 97 143 96 773.1 0 96 144 85 773.1 0 85 145 91 773.1 0 91 146 94 773.1 0 94 147 102 773.1 0 102 148 101 773.1 0 101 149 192 773.1 0 192 150 121 773.1 0 121 151 108 773.1 0 108 152 111 773.1 0 111 153 167 773.1 0 167 154 1242 773.1 0 1242 155 1621 773.1 0 1621 156 3881 773.1 0 3881 157 2541 773.1 0 2541 158 941 773.1 0 941 159 185 773.1 0 185 160 60 773.1 0 60 161 49 773.1 0 49 162 51 773.1 0 51 163 95 773.1 0 95 164 124 773.1 0 124 165 71 773.1 0 71 166 59 773.1 0 59 167 154 773.1 0 154 168 148 773.1 0 148 169 164 773.1 0 164 170 131 773.1 0 131 171 90 773.1 0 90 172 88 773.1 0 88 173 70 773.1 0 70 174 76 773.1 0 76 175 71 773.1 0 71 176 68 773.1 0 68 177 65 773.1 0 65 178 423 773.1 0 423 179 59 773.1 0 59 180 82 773.1 0 82 181 46 773.1 0 46 182 59 773.1 0 59 183 61 773.1 0 61 184 81 773.1 0 81 185 88 773.1 0 88 186 86 773.1 0 86 187 67 773.1 0 67 188 137 773.1 0 137 189 78 773.1 0 78 190 107 773.1 0 107 191 73 773.1 0 73 192 78 773.1 0 78 193 72 773.1 0 72 194 73 773.1 0 73 195 61 773.1 0 61 196 75 773.1 0 75 197 80 773.1 0 80 198 77 773.1 0 77 199 62 773.1 0 62 200 227 773.1 0 227 201 117 773.1 0 117 202 198 773.1 0 198 203 69 773.1 0 69 204 100 773.1 0 100 205 76 773.1 0 76 206 69 773.1 0 69 207 71 773.1 0 71 208 69 773.1 0 69 209 72 773.1 0 72 210 65 773.1 0 65 211 108 773.1 0 108 212 98 773.1 0 98 213 101 773.1 0 101 214 118 773.1 0 118 215 118 773.1 0 118 216 105 773.1 0 105 217 68 773.1 0 68 218 116 773.1 0 116 219 73 773.1 0 73 220 62 773.1 0 62 221 69 773.1 0 69 222 79 773.1 0 79 223 77 773.1 0 77 224 77 773.1 0 77 225 76 773.1 0 76 226 68 773.1 0 68 227 73 773.1 0 73 228 309 773.1 0 309 229 268 773.1 0 268 230 392 773.1 0 392 231 112 773.1 0 112 232 93 773.1 0 93 233 106 773.1 0 106 234 99 773.1 0 99 235 110 773.1 0 110 236 130 773.1 0 130 237 111 773.1 0 111 238 80 773.1 0 80 239 95 773.1 0 95 240 80 773.1 0 80 241 46 773.1 0 46 242 80 773.1 0 80 243 124 773.1 0 124 244 94 773.1 0 94 245 78 773.1 0 78 246 59 773.1 0 59 247 107 773.1 0 107 248 147 773.1 0 147 249 411 773.1 0 411 250 72 773.1 0 72 251 93 773.1 0 93 252 88 773.1 0 88 253 71 773.1 0 71 254 74 773.1 0 74 255 116 773.1 0 116 256 100 773.1 0 100 257 140 773.1 0 140 258 102 773.1 0 102 259 62 773.1 0 62 260 66 773.1 0 66 261 64 773.1 0 64 262 73 773.1 0 73 263 90 773.1 0 90 264 69 773.1 0 69 265 64 773.1 0 64 266 73 773.1 0 73 267 74 773.1 0 74 268 114 773.1 0 114 269 181 773.1 0 181 270 218 773.1 0 218 271 152 773.1 0 152 272 82 773.1 0 82 273 74 773.1 0 74 274 90 773.1 0 90 275 65 773.1 0 65 276 127 773.1 0 127 277 111 773.1 0 111 278 73 773.1 0 73 279 84 773.1 0 84 280 86 773.1 0 86 281 133 773.1 0 133 282 138 773.1 0 138 283 135 773.1 0 135 284 140 773.1 0 140 285 302 773.1 0 302 286 139 773.1 0 139 287 119 773.1 0 119 288 160 773.1 0 160 289 125 773.1 0 125 290 126 773.1 0 126 291 164 773.1 0 164 292 132 773.1 0 132 293 138 773.1 0 138 294 459 773.1 0 459 295 973 773.1 0 973 296 2559 773.1 0 2559 297 1728 773.1 0 1728 298 4651 773.1 0 4651 299 7383 773.1 0 7383 300 7678 773.1 0 7678 301 210 773.1 0 210 Finished in 7.65 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 112,986 Reads with adapters: 1,691 (1.5%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 1,670 (1.5%) Total basepairs processed: 26,926,971 bp Total written (filtered): 451,949 bp (1.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1691 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 20 0.0 3 0 20 20 1624 0.0 4 1593 28 3 21 4 0.0 4 0 1 1 1 1 22 1 0.0 4 0 0 1 23 2 0.0 4 0 0 0 2 24 1 0.0 4 1 50 1 0.0 4 1 55 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 82 1 0.0 4 1 83 1 0.0 4 0 0 0 1 93 1 0.0 4 1 121 8 0.0 4 0 0 0 0 8 123 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 1 153 1 0.0 4 0 0 0 1 155 1 0.0 4 0 0 0 0 1 163 3 0.0 4 0 0 0 0 3 169 6 0.0 4 0 0 0 0 6 176 1 0.0 4 1 198 2 0.0 4 2 206 1 0.0 4 0 1 212 1 0.0 4 0 0 1 228 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 1,670 Reads with adapters: 179 (10.7%) Reads written (passing filters): 1,670 (100.0%) Total basepairs processed: 451,949 bp Total written (filtered): 445,154 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 179 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.2% G: 96.6% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 6.5 0 1 6 1 0.4 0 1 9 69 0.0 0 0 69 10 62 0.0 1 2 60 11 26 0.0 1 0 26 145 1 0.0 1 1 269 17 0.0 1 15 2 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.