This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 145.61 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 352,733 (11.1%) Reads that were too short: 11,069 (0.3%) Reads written (passing filters): 341,664 (10.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 78,483,603 bp (8.2%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 352733 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 786 773.1 0 786 7 1975 773.1 0 1975 8 52243 773.1 0 52243 9 45491 773.1 0 45491 10 103 773.1 0 103 11 52 773.1 0 52 12 64 773.1 0 64 13 43 773.1 0 43 14 39 773.1 0 39 15 32 773.1 0 32 16 37 773.1 0 37 17 30 773.1 0 30 18 34 773.1 0 34 19 35 773.1 0 35 20 38 773.1 0 38 21 49 773.1 0 49 22 35 773.1 0 35 23 41 773.1 0 41 24 107 773.1 0 107 25 82 773.1 0 82 26 73 773.1 0 73 27 42 773.1 0 42 28 49 773.1 0 49 29 29 773.1 0 29 30 62 773.1 0 62 31 112 773.1 0 112 32 53 773.1 0 53 33 46 773.1 0 46 34 107 773.1 0 107 35 88 773.1 0 88 36 88 773.1 0 88 37 410 773.1 0 410 38 82 773.1 0 82 39 56 773.1 0 56 40 76 773.1 0 76 41 52 773.1 0 52 42 55 773.1 0 55 43 59 773.1 0 59 44 51 773.1 0 51 45 52 773.1 0 52 46 85 773.1 0 85 47 114 773.1 0 114 48 49 773.1 0 49 49 52 773.1 0 52 50 44 773.1 0 44 51 86 773.1 0 86 52 60 773.1 0 60 53 139 773.1 0 139 54 257 773.1 0 257 55 334 773.1 0 334 56 70 773.1 0 70 57 66 773.1 0 66 58 68 773.1 0 68 59 29 773.1 0 29 60 47 773.1 0 47 61 70 773.1 0 70 62 68 773.1 0 68 63 110 773.1 0 110 64 213 773.1 0 213 65 89 773.1 0 89 66 158 773.1 0 158 67 125 773.1 0 125 68 125 773.1 0 125 69 304 773.1 0 304 70 123 773.1 0 123 71 116 773.1 0 116 72 88 773.1 0 88 73 79 773.1 0 79 74 140 773.1 0 140 75 174 773.1 0 174 76 330 773.1 0 330 77 407 773.1 0 407 78 637 773.1 0 637 79 1533 773.1 0 1533 80 3996 773.1 0 3996 81 63065 773.1 0 63065 82 43255 773.1 0 43255 83 41612 773.1 0 41612 84 228 773.1 0 228 85 319 773.1 0 319 86 318 773.1 0 318 87 589 773.1 0 589 88 60 773.1 0 60 89 44 773.1 0 44 90 39 773.1 0 39 91 34 773.1 0 34 92 41 773.1 0 41 93 34 773.1 0 34 94 48 773.1 0 48 95 55 773.1 0 55 96 55 773.1 0 55 97 50 773.1 0 50 98 83 773.1 0 83 99 57 773.1 0 57 100 86 773.1 0 86 101 62 773.1 0 62 102 143 773.1 0 143 103 76 773.1 0 76 104 174 773.1 0 174 105 73 773.1 0 73 106 87 773.1 0 87 107 92 773.1 0 92 108 175 773.1 0 175 109 276 773.1 0 276 110 97 773.1 0 97 111 219 773.1 0 219 112 168 773.1 0 168 113 90 773.1 0 90 114 115 773.1 0 115 115 162 773.1 0 162 116 140 773.1 0 140 117 202 773.1 0 202 118 213 773.1 0 213 119 778 773.1 0 778 120 696 773.1 0 696 121 1120 773.1 0 1120 122 21223 773.1 0 21223 123 12723 773.1 0 12723 124 21545 773.1 0 21545 125 194 773.1 0 194 126 173 773.1 0 173 127 83 773.1 0 83 128 59 773.1 0 59 129 67 773.1 0 67 130 38 773.1 0 38 131 54 773.1 0 54 132 44 773.1 0 44 133 54 773.1 0 54 134 62 773.1 0 62 135 56 773.1 0 56 136 52 773.1 0 52 137 58 773.1 0 58 138 62 773.1 0 62 139 81 773.1 0 81 140 56 773.1 0 56 141 80 773.1 0 80 142 534 773.1 0 534 143 415 773.1 0 415 144 1029 773.1 0 1029 145 577 773.1 0 577 146 744 773.1 0 744 147 304 773.1 0 304 148 115 773.1 0 115 149 245 773.1 0 245 150 136 773.1 0 136 151 461 773.1 0 461 152 95 773.1 0 95 153 202 773.1 0 202 154 86 773.1 0 86 155 76 773.1 0 76 156 46 773.1 0 46 157 25 773.1 0 25 158 40 773.1 0 40 159 40 773.1 0 40 160 63 773.1 0 63 161 58 773.1 0 58 162 45 773.1 0 45 163 50 773.1 0 50 164 58 773.1 0 58 165 38 773.1 0 38 166 78 773.1 0 78 167 302 773.1 0 302 168 54 773.1 0 54 169 169 773.1 0 169 170 125 773.1 0 125 171 89 773.1 0 89 172 39 773.1 0 39 173 57 773.1 0 57 174 59 773.1 0 59 175 76 773.1 0 76 176 47 773.1 0 47 177 51 773.1 0 51 178 47 773.1 0 47 179 60 773.1 0 60 180 113 773.1 0 113 181 223 773.1 0 223 182 3079 773.1 0 3079 183 1970 773.1 0 1970 184 3040 773.1 0 3040 185 93 773.1 0 93 186 68 773.1 0 68 187 78 773.1 0 78 188 44 773.1 0 44 189 39 773.1 0 39 190 52 773.1 0 52 191 38 773.1 0 38 192 32 773.1 0 32 193 77 773.1 0 77 194 129 773.1 0 129 195 107 773.1 0 107 196 63 773.1 0 63 197 35 773.1 0 35 198 91 773.1 0 91 199 69 773.1 0 69 200 97 773.1 0 97 201 55 773.1 0 55 202 30 773.1 0 30 203 33 773.1 0 33 204 24 773.1 0 24 205 59 773.1 0 59 206 67 773.1 0 67 207 54 773.1 0 54 208 34 773.1 0 34 209 36 773.1 0 36 210 66 773.1 0 66 211 60 773.1 0 60 212 57 773.1 0 57 213 33 773.1 0 33 214 44 773.1 0 44 215 29 773.1 0 29 216 35 773.1 0 35 217 31 773.1 0 31 218 38 773.1 0 38 219 59 773.1 0 59 220 62 773.1 0 62 221 41 773.1 0 41 222 35 773.1 0 35 223 37 773.1 0 37 224 36 773.1 0 36 225 45 773.1 0 45 226 67 773.1 0 67 227 84 773.1 0 84 228 109 773.1 0 109 229 56 773.1 0 56 230 40 773.1 0 40 231 50 773.1 0 50 232 34 773.1 0 34 233 41 773.1 0 41 234 29 773.1 0 29 235 31 773.1 0 31 236 35 773.1 0 35 237 46 773.1 0 46 238 52 773.1 0 52 239 38 773.1 0 38 240 198 773.1 0 198 241 122 773.1 0 122 242 108 773.1 0 108 243 34 773.1 0 34 244 39 773.1 0 39 245 50 773.1 0 50 246 39 773.1 0 39 247 47 773.1 0 47 248 54 773.1 0 54 249 61 773.1 0 61 250 36 773.1 0 36 251 37 773.1 0 37 252 46 773.1 0 46 253 69 773.1 0 69 254 62 773.1 0 62 255 84 773.1 0 84 256 61 773.1 0 61 257 62 773.1 0 62 258 76 773.1 0 76 259 93 773.1 0 93 260 103 773.1 0 103 261 194 773.1 0 194 262 108 773.1 0 108 263 182 773.1 0 182 264 135 773.1 0 135 265 90 773.1 0 90 266 768 773.1 0 768 267 634 773.1 0 634 268 904 773.1 0 904 269 160 773.1 0 160 270 76 773.1 0 76 271 46 773.1 0 46 272 57 773.1 0 57 273 66 773.1 0 66 274 264 773.1 0 264 275 282 773.1 0 282 276 374 773.1 0 374 277 158 773.1 0 158 278 81 773.1 0 81 279 140 773.1 0 140 280 127 773.1 0 127 281 193 773.1 0 193 282 69 773.1 0 69 283 72 773.1 0 72 284 36 773.1 0 36 285 146 773.1 0 146 286 263 773.1 0 263 287 279 773.1 0 279 288 233 773.1 0 233 289 158 773.1 0 158 290 176 773.1 0 176 291 66 773.1 0 66 292 108 773.1 0 108 293 152 773.1 0 152 294 224 773.1 0 224 295 189 773.1 0 189 296 139 773.1 0 139 297 70 773.1 0 70 298 100 773.1 0 100 299 96 773.1 0 96 300 109 773.1 0 109 301 107 773.1 0 107 Finished in 20.38 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 341,664 Reads with adapters: 54,745 (16.0%) Reads that were too short: 443 (0.1%) Reads written (passing filters): 54,302 (15.9%) Total basepairs processed: 78,483,603 bp Total written (filtered): 14,779,734 bp (18.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 54745 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 18 0.0 3 0 0 9 9 16 403 0.0 3 0 349 44 10 17 52104 0.0 3 49418 2250 367 69 18 149 0.0 3 1 136 9 3 19 138 0.0 3 4 2 83 49 20 116 0.0 3 2 2 1 111 22 49 0.0 3 0 0 1 48 25 19 0.0 3 0 0 8 11 28 48 0.0 3 0 0 0 48 29 1 0.0 3 0 0 0 1 30 1 0.0 3 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 281 0.0 3 0 0 0 281 35 1 0.0 3 1 38 2 0.0 3 0 0 1 1 41 2 0.0 3 0 0 0 2 44 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 1 47 10 0.0 3 0 0 0 10 48 5 0.0 3 0 0 0 5 49 117 0.0 3 0 0 3 114 50 565 0.0 3 0 0 3 562 51 35 0.0 3 0 0 0 35 52 35 0.0 3 0 0 2 33 53 68 0.0 3 0 0 3 65 54 5 0.0 3 0 0 0 5 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 8 0.0 3 0 0 0 8 63 5 0.0 3 0 0 0 5 64 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 69 7 0.0 3 0 0 0 7 70 4 0.0 3 0 0 0 4 71 4 0.0 3 0 0 0 4 72 3 0.0 3 0 0 0 3 73 9 0.0 3 0 0 1 8 75 4 0.0 3 0 0 0 4 76 13 0.0 3 0 0 0 13 77 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 1 1 81 4 0.0 3 0 0 0 4 82 4 0.0 3 0 0 2 2 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 5 0.0 3 0 0 0 5 91 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 19 0.0 3 0 0 1 18 102 5 0.0 3 0 0 0 5 103 2 0.0 3 0 0 0 2 106 19 0.0 3 0 0 0 19 107 6 0.0 3 0 0 0 6 109 3 0.0 3 0 0 0 3 110 12 0.0 3 0 0 0 12 114 1 0.0 3 0 0 0 1 118 6 0.0 3 0 0 1 5 121 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 1 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 3 0.0 3 0 0 0 3 152 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 157 6 0.0 3 0 0 0 6 158 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 161 6 0.0 3 0 0 0 6 162 90 0.0 3 0 0 3 87 163 39 0.0 3 0 0 0 39 164 21 0.0 3 0 0 0 21 165 102 0.0 3 0 0 8 94 166 14 0.0 3 0 0 0 14 167 5 0.0 3 0 0 0 5 169 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 0 3 172 1 0.0 3 0 0 0 1 177 2 0.0 3 0 1 0 1 179 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 1 188 1 0.0 3 0 1 192 4 0.0 3 0 0 0 4 193 3 0.0 3 0 0 0 3 194 7 0.0 3 0 0 1 6 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 208 5 0.0 3 0 0 0 5 209 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 4 0.0 3 0 0 0 4 282 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.02 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 54,302 Reads with adapters: 289 (0.5%) Reads written (passing filters): 54,302 (100.0%) Total basepairs processed: 14,779,734 bp Total written (filtered): 14,778,808 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 289 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.6% C: 10.7% G: 21.1% T: 6.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 242 848.5 0 242 4 35 212.1 0 35 5 12 53.0 0 12