This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 145.27 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 371,589 (11.7%) Reads that were too short: 9,697 (0.3%) Reads written (passing filters): 361,892 (11.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 83,081,956 bp (8.7%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 371589 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 609 773.1 0 609 7 1166 773.1 0 1166 8 48661 773.1 0 48661 9 53873 773.1 0 53873 10 81 773.1 0 81 11 65 773.1 0 65 12 34 773.1 0 34 13 55 773.1 0 55 14 41 773.1 0 41 15 40 773.1 0 40 16 43 773.1 0 43 17 39 773.1 0 39 18 56 773.1 0 56 19 49 773.1 0 49 20 35 773.1 0 35 21 45 773.1 0 45 22 50 773.1 0 50 23 33 773.1 0 33 24 54 773.1 0 54 25 40 773.1 0 40 26 44 773.1 0 44 27 49 773.1 0 49 28 66 773.1 0 66 29 30 773.1 0 30 30 73 773.1 0 73 31 98 773.1 0 98 32 60 773.1 0 60 33 60 773.1 0 60 34 75 773.1 0 75 35 72 773.1 0 72 36 66 773.1 0 66 37 462 773.1 0 462 38 84 773.1 0 84 39 84 773.1 0 84 40 70 773.1 0 70 41 55 773.1 0 55 42 74 773.1 0 74 43 48 773.1 0 48 44 60 773.1 0 60 45 53 773.1 0 53 46 92 773.1 0 92 47 140 773.1 0 140 48 55 773.1 0 55 49 71 773.1 0 71 50 53 773.1 0 53 51 94 773.1 0 94 52 80 773.1 0 80 53 127 773.1 0 127 54 228 773.1 0 228 55 178 773.1 0 178 56 66 773.1 0 66 57 71 773.1 0 71 58 101 773.1 0 101 59 39 773.1 0 39 60 65 773.1 0 65 61 98 773.1 0 98 62 73 773.1 0 73 63 126 773.1 0 126 64 268 773.1 0 268 65 105 773.1 0 105 66 168 773.1 0 168 67 134 773.1 0 134 68 115 773.1 0 115 69 347 773.1 0 347 70 129 773.1 0 129 71 81 773.1 0 81 72 94 773.1 0 94 73 100 773.1 0 100 74 145 773.1 0 145 75 203 773.1 0 203 76 304 773.1 0 304 77 366 773.1 0 366 78 653 773.1 0 653 79 1488 773.1 0 1488 80 3448 773.1 0 3448 81 71104 773.1 0 71104 82 47476 773.1 0 47476 83 45762 773.1 0 45762 84 244 773.1 0 244 85 249 773.1 0 249 86 120 773.1 0 120 87 305 773.1 0 305 88 69 773.1 0 69 89 51 773.1 0 51 90 56 773.1 0 56 91 44 773.1 0 44 92 38 773.1 0 38 93 44 773.1 0 44 94 47 773.1 0 47 95 64 773.1 0 64 96 78 773.1 0 78 97 51 773.1 0 51 98 81 773.1 0 81 99 73 773.1 0 73 100 76 773.1 0 76 101 85 773.1 0 85 102 128 773.1 0 128 103 102 773.1 0 102 104 130 773.1 0 130 105 80 773.1 0 80 106 141 773.1 0 141 107 134 773.1 0 134 108 193 773.1 0 193 109 337 773.1 0 337 110 88 773.1 0 88 111 247 773.1 0 247 112 205 773.1 0 205 113 103 773.1 0 103 114 122 773.1 0 122 115 209 773.1 0 209 116 189 773.1 0 189 117 202 773.1 0 202 118 186 773.1 0 186 119 491 773.1 0 491 120 515 773.1 0 515 121 730 773.1 0 730 122 21801 773.1 0 21801 123 12455 773.1 0 12455 124 21899 773.1 0 21899 125 147 773.1 0 147 126 100 773.1 0 100 127 75 773.1 0 75 128 68 773.1 0 68 129 75 773.1 0 75 130 66 773.1 0 66 131 68 773.1 0 68 132 49 773.1 0 49 133 53 773.1 0 53 134 43 773.1 0 43 135 58 773.1 0 58 136 60 773.1 0 60 137 73 773.1 0 73 138 58 773.1 0 58 139 73 773.1 0 73 140 65 773.1 0 65 141 88 773.1 0 88 142 589 773.1 0 589 143 397 773.1 0 397 144 1014 773.1 0 1014 145 504 773.1 0 504 146 579 773.1 0 579 147 293 773.1 0 293 148 73 773.1 0 73 149 132 773.1 0 132 150 102 773.1 0 102 151 300 773.1 0 300 152 107 773.1 0 107 153 182 773.1 0 182 154 62 773.1 0 62 155 62 773.1 0 62 156 35 773.1 0 35 157 36 773.1 0 36 158 46 773.1 0 46 159 44 773.1 0 44 160 70 773.1 0 70 161 61 773.1 0 61 162 48 773.1 0 48 163 53 773.1 0 53 164 65 773.1 0 65 165 77 773.1 0 77 166 64 773.1 0 64 167 305 773.1 0 305 168 79 773.1 0 79 169 162 773.1 0 162 170 103 773.1 0 103 171 86 773.1 0 86 172 58 773.1 0 58 173 54 773.1 0 54 174 43 773.1 0 43 175 64 773.1 0 64 176 58 773.1 0 58 177 72 773.1 0 72 178 81 773.1 0 81 179 78 773.1 0 78 180 113 773.1 0 113 181 226 773.1 0 226 182 3639 773.1 0 3639 183 2252 773.1 0 2252 184 3723 773.1 0 3723 185 88 773.1 0 88 186 69 773.1 0 69 187 65 773.1 0 65 188 35 773.1 0 35 189 62 773.1 0 62 190 63 773.1 0 63 191 31 773.1 0 31 192 48 773.1 0 48 193 64 773.1 0 64 194 77 773.1 0 77 195 67 773.1 0 67 196 59 773.1 0 59 197 43 773.1 0 43 198 58 773.1 0 58 199 70 773.1 0 70 200 70 773.1 0 70 201 52 773.1 0 52 202 57 773.1 0 57 203 44 773.1 0 44 204 31 773.1 0 31 205 54 773.1 0 54 206 71 773.1 0 71 207 48 773.1 0 48 208 37 773.1 0 37 209 56 773.1 0 56 210 36 773.1 0 36 211 50 773.1 0 50 212 49 773.1 0 49 213 38 773.1 0 38 214 51 773.1 0 51 215 49 773.1 0 49 216 34 773.1 0 34 217 39 773.1 0 39 218 53 773.1 0 53 219 68 773.1 0 68 220 66 773.1 0 66 221 55 773.1 0 55 222 43 773.1 0 43 223 42 773.1 0 42 224 54 773.1 0 54 225 53 773.1 0 53 226 60 773.1 0 60 227 57 773.1 0 57 228 64 773.1 0 64 229 68 773.1 0 68 230 52 773.1 0 52 231 57 773.1 0 57 232 28 773.1 0 28 233 45 773.1 0 45 234 49 773.1 0 49 235 35 773.1 0 35 236 57 773.1 0 57 237 78 773.1 0 78 238 58 773.1 0 58 239 47 773.1 0 47 240 211 773.1 0 211 241 128 773.1 0 128 242 104 773.1 0 104 243 41 773.1 0 41 244 34 773.1 0 34 245 66 773.1 0 66 246 40 773.1 0 40 247 46 773.1 0 46 248 42 773.1 0 42 249 46 773.1 0 46 250 42 773.1 0 42 251 50 773.1 0 50 252 49 773.1 0 49 253 64 773.1 0 64 254 62 773.1 0 62 255 70 773.1 0 70 256 66 773.1 0 66 257 61 773.1 0 61 258 79 773.1 0 79 259 63 773.1 0 63 260 76 773.1 0 76 261 112 773.1 0 112 262 76 773.1 0 76 263 144 773.1 0 144 264 118 773.1 0 118 265 110 773.1 0 110 266 745 773.1 0 745 267 522 773.1 0 522 268 855 773.1 0 855 269 140 773.1 0 140 270 57 773.1 0 57 271 58 773.1 0 58 272 59 773.1 0 59 273 49 773.1 0 49 274 220 773.1 0 220 275 219 773.1 0 219 276 322 773.1 0 322 277 62 773.1 0 62 278 72 773.1 0 72 279 72 773.1 0 72 280 65 773.1 0 65 281 135 773.1 0 135 282 81 773.1 0 81 283 75 773.1 0 75 284 56 773.1 0 56 285 96 773.1 0 96 286 220 773.1 0 220 287 129 773.1 0 129 288 150 773.1 0 150 289 98 773.1 0 98 290 95 773.1 0 95 291 48 773.1 0 48 292 95 773.1 0 95 293 114 773.1 0 114 294 155 773.1 0 155 295 190 773.1 0 190 296 102 773.1 0 102 297 68 773.1 0 68 298 79 773.1 0 79 299 79 773.1 0 79 300 99 773.1 0 99 301 83 773.1 0 83 Finished in 23.13 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 361,892 Reads with adapters: 57,144 (15.8%) Reads that were too short: 53 (0.0%) Reads written (passing filters): 57,091 (15.8%) Total basepairs processed: 83,081,956 bp Total written (filtered): 15,336,982 bp (18.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 57144 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 18 27 0.0 3 0 0 27 19 819 0.0 3 0 802 13 1 3 20 54158 0.0 4 53281 846 24 5 2 21 93 0.0 4 13 74 5 1 22 121 0.0 4 116 0 3 1 1 23 37 0.0 4 0 0 1 34 2 24 3 0.0 4 0 0 0 0 3 54 1 0.0 4 1 63 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 1 77 1 0.0 4 0 0 0 1 81 1 0.0 4 0 0 0 0 1 90 1 0.0 4 1 92 1 0.0 4 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 99 8 0.0 4 0 0 0 0 8 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 17 0.0 4 0 0 0 0 17 127 1784 0.0 4 0 0 0 0 1784 128 9 0.0 4 0 0 0 0 9 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 147 1 0.0 4 1 148 4 0.0 4 0 0 0 0 4 149 7 0.0 4 0 0 0 1 6 150 5 0.0 4 0 0 0 1 4 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 4 0.0 4 1 0 0 0 3 177 1 0.0 4 0 0 0 0 1 179 4 0.0 4 0 0 0 0 4 180 1 0.0 4 0 0 0 0 1 188 1 0.0 4 1 190 1 0.0 4 0 0 0 0 1 191 1 0.0 4 1 200 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.06 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 57,091 Reads with adapters: 7,742 (13.6%) Reads written (passing filters): 57,091 (100.0%) Total basepairs processed: 15,336,982 bp Total written (filtered): 15,262,472 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7742 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.5% G: 95.6% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 892.0 0 11 4 8 223.0 0 8 5 4 55.8 0 4 6 4 13.9 0 4 7 2 3.5 0 2 8 6 0.9 0 6 9 4367 0.2 0 100 4267 10 2908 0.1 1 97 2811 11 420 0.0 1 2 418 12 3 0.0 1 0 3 13 1 0.0 1 0 1 28 1 0.0 1 1 169 5 0.0 1 5 191 1 0.0 1 0 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.