This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.17 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 696,531 (22.0%) Reads that were too short: 568,273 (17.9%) Reads written (passing filters): 128,258 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,253,536 bp (2.2%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 696531 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2587 773.1 0 2587 7 14955 773.1 0 14955 8 383 773.1 0 383 9 14192 773.1 0 14192 10 89 773.1 0 89 11 50 773.1 0 50 12 50 773.1 0 50 13 48 773.1 0 48 14 44 773.1 0 44 15 44 773.1 0 44 16 35 773.1 0 35 17 47 773.1 0 47 18 41 773.1 0 41 19 35 773.1 0 35 20 35 773.1 0 35 21 46 773.1 0 46 22 32 773.1 0 32 23 33 773.1 0 33 24 28 773.1 0 28 25 41 773.1 0 41 26 27 773.1 0 27 27 27 773.1 0 27 28 26 773.1 0 26 29 44 773.1 0 44 30 44 773.1 0 44 31 122 773.1 0 122 32 155 773.1 0 155 33 40 773.1 0 40 34 85 773.1 0 85 35 133 773.1 0 133 36 46 773.1 0 46 37 48 773.1 0 48 38 48 773.1 0 48 39 75 773.1 0 75 40 34 773.1 0 34 41 42 773.1 0 42 42 31 773.1 0 31 43 54 773.1 0 54 44 32 773.1 0 32 45 50 773.1 0 50 46 33 773.1 0 33 47 31 773.1 0 31 48 36 773.1 0 36 49 29 773.1 0 29 50 41 773.1 0 41 51 26 773.1 0 26 52 31 773.1 0 31 53 55 773.1 0 55 54 51 773.1 0 51 55 43 773.1 0 43 56 69 773.1 0 69 57 58 773.1 0 58 58 53 773.1 0 53 59 68 773.1 0 68 60 117 773.1 0 117 61 39 773.1 0 39 62 29 773.1 0 29 63 27 773.1 0 27 64 34 773.1 0 34 65 62 773.1 0 62 66 33 773.1 0 33 67 45 773.1 0 45 68 42 773.1 0 42 69 50 773.1 0 50 70 32 773.1 0 32 71 53 773.1 0 53 72 37 773.1 0 37 73 35 773.1 0 35 74 38 773.1 0 38 75 101 773.1 0 101 76 113 773.1 0 113 77 97 773.1 0 97 78 33 773.1 0 33 79 42 773.1 0 42 80 28 773.1 0 28 81 37 773.1 0 37 82 39 773.1 0 39 83 35 773.1 0 35 84 36 773.1 0 36 85 35 773.1 0 35 86 36 773.1 0 36 87 42 773.1 0 42 88 47 773.1 0 47 89 65 773.1 0 65 90 55 773.1 0 55 91 57 773.1 0 57 92 78 773.1 0 78 93 38 773.1 0 38 94 51 773.1 0 51 95 48 773.1 0 48 96 50 773.1 0 50 97 48 773.1 0 48 98 49 773.1 0 49 99 52 773.1 0 52 100 93 773.1 0 93 101 40 773.1 0 40 102 54 773.1 0 54 103 84 773.1 0 84 104 58 773.1 0 58 105 45 773.1 0 45 106 48 773.1 0 48 107 57 773.1 0 57 108 56 773.1 0 56 109 42 773.1 0 42 110 44 773.1 0 44 111 52 773.1 0 52 112 77 773.1 0 77 113 1150 773.1 0 1150 114 580 773.1 0 580 115 655 773.1 0 655 116 110 773.1 0 110 117 58 773.1 0 58 118 55 773.1 0 55 119 42 773.1 0 42 120 62 773.1 0 62 121 108 773.1 0 108 122 108 773.1 0 108 123 109 773.1 0 109 124 79 773.1 0 79 125 67 773.1 0 67 126 117 773.1 0 117 127 62 773.1 0 62 128 161 773.1 0 161 129 411 773.1 0 411 130 109 773.1 0 109 131 56 773.1 0 56 132 61 773.1 0 61 133 91 773.1 0 91 134 130 773.1 0 130 135 109 773.1 0 109 136 86 773.1 0 86 137 119 773.1 0 119 138 96 773.1 0 96 139 111 773.1 0 111 140 126 773.1 0 126 141 133 773.1 0 133 142 207 773.1 0 207 143 182 773.1 0 182 144 223 773.1 0 223 145 183 773.1 0 183 146 237 773.1 0 237 147 5481 773.1 0 5481 148 4196 773.1 0 4196 149 4903 773.1 0 4903 150 299 773.1 0 299 151 58 773.1 0 58 152 47 773.1 0 47 153 100 773.1 0 100 154 61 773.1 0 61 155 91 773.1 0 91 156 82 773.1 0 82 157 95 773.1 0 95 158 81 773.1 0 81 159 75 773.1 0 75 160 76 773.1 0 76 161 154 773.1 0 154 162 127 773.1 0 127 163 87 773.1 0 87 164 87 773.1 0 87 165 82 773.1 0 82 166 54 773.1 0 54 167 52 773.1 0 52 168 72 773.1 0 72 169 105 773.1 0 105 170 67 773.1 0 67 171 58 773.1 0 58 172 68 773.1 0 68 173 88 773.1 0 88 174 84 773.1 0 84 175 93 773.1 0 93 176 138 773.1 0 138 177 77 773.1 0 77 178 108 773.1 0 108 179 145 773.1 0 145 180 137 773.1 0 137 181 222 773.1 0 222 182 182 773.1 0 182 183 174 773.1 0 174 184 140 773.1 0 140 185 185 773.1 0 185 186 249 773.1 0 249 187 365 773.1 0 365 188 299 773.1 0 299 189 1336 773.1 0 1336 190 1542 773.1 0 1542 191 1511 773.1 0 1511 192 1169 773.1 0 1169 193 650 773.1 0 650 194 523 773.1 0 523 195 799 773.1 0 799 196 611 773.1 0 611 197 755 773.1 0 755 198 1127 773.1 0 1127 199 4943 773.1 0 4943 200 30856 773.1 0 30856 201 19562 773.1 0 19562 202 28647 773.1 0 28647 203 3101 773.1 0 3101 204 2808 773.1 0 2808 205 66529 773.1 0 66529 206 61473 773.1 0 61473 207 81917 773.1 0 81917 208 22481 773.1 0 22481 209 6320 773.1 0 6320 210 1864 773.1 0 1864 211 2801 773.1 0 2801 212 1471 773.1 0 1471 213 1947 773.1 0 1947 214 936 773.1 0 936 215 2236 773.1 0 2236 216 1199 773.1 0 1199 217 2388 773.1 0 2388 218 1675 773.1 0 1675 219 7889 773.1 0 7889 220 6207 773.1 0 6207 221 7068 773.1 0 7068 222 5430 773.1 0 5430 223 3481 773.1 0 3481 224 2438 773.1 0 2438 225 16698 773.1 0 16698 226 41254 773.1 0 41254 227 39374 773.1 0 39374 228 32244 773.1 0 32244 229 134 773.1 0 134 230 59 773.1 0 59 231 96 773.1 0 96 232 43 773.1 0 43 233 26 773.1 0 26 234 46 773.1 0 46 235 97 773.1 0 97 236 73 773.1 0 73 237 65 773.1 0 65 238 143 773.1 0 143 239 77 773.1 0 77 240 56 773.1 0 56 241 57 773.1 0 57 242 51 773.1 0 51 243 52 773.1 0 52 244 125 773.1 0 125 245 172 773.1 0 172 246 303 773.1 0 303 247 296 773.1 0 296 248 527 773.1 0 527 249 1042 773.1 0 1042 250 217 773.1 0 217 251 483 773.1 0 483 252 379 773.1 0 379 253 476 773.1 0 476 254 242 773.1 0 242 255 458 773.1 0 458 256 242 773.1 0 242 257 316 773.1 0 316 258 336 773.1 0 336 259 923 773.1 0 923 260 1135 773.1 0 1135 261 1326 773.1 0 1326 262 32713 773.1 0 32713 263 25337 773.1 0 25337 264 44979 773.1 0 44979 265 317 773.1 0 317 266 64 773.1 0 64 267 32 773.1 0 32 268 31 773.1 0 31 269 56 773.1 0 56 270 109 773.1 0 109 271 87 773.1 0 87 272 35 773.1 0 35 273 71 773.1 0 71 274 58 773.1 0 58 275 107 773.1 0 107 276 46 773.1 0 46 277 43 773.1 0 43 278 68 773.1 0 68 279 35 773.1 0 35 280 27 773.1 0 27 281 29 773.1 0 29 282 46 773.1 0 46 283 188 773.1 0 188 284 265 773.1 0 265 285 57 773.1 0 57 286 20 773.1 0 20 287 17 773.1 0 17 288 22 773.1 0 22 289 40 773.1 0 40 290 32 773.1 0 32 291 30 773.1 0 30 292 305 773.1 0 305 293 254 773.1 0 254 294 501 773.1 0 501 295 69 773.1 0 69 296 39 773.1 0 39 297 46 773.1 0 46 298 44 773.1 0 44 299 26 773.1 0 26 300 30 773.1 0 30 301 49 773.1 0 49 Finished in 7.38 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 128,258 Reads with adapters: 17,741 (13.8%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 17,696 (13.8%) Total basepairs processed: 21,253,536 bp Total written (filtered): 4,703,666 bp (22.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17741 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 207 0.0 3 0 200 6 0 1 20 16086 0.0 4 15825 255 6 21 36 0.0 4 6 28 2 22 5 0.0 4 1 0 3 0 1 23 9 0.0 4 0 0 0 8 1 24 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 36 3 0.0 4 0 0 0 0 3 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 65 1 0.0 4 0 0 0 0 1 66 3 0.0 4 0 0 0 0 3 91 2 0.0 4 0 0 0 0 2 92 16 0.0 4 0 0 0 1 15 95 1 0.0 4 0 0 0 0 1 104 3 0.0 4 0 0 0 0 3 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 112 1 0.0 4 1 120 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 20 0.0 4 0 0 0 0 20 127 1311 0.0 4 0 0 0 0 1311 128 4 0.0 4 0 0 0 0 4 130 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 193 2 0.0 4 0 0 0 0 2 223 1 0.0 4 0 1 228 3 0.0 4 0 0 0 0 3 229 4 0.0 4 0 1 1 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.89 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 17,696 Reads with adapters: 199 (1.1%) Reads written (passing filters): 17,696 (100.0%) Total basepairs processed: 4,703,666 bp Total written (filtered): 4,700,861 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 199 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 19.1% C: 7.0% G: 71.4% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 42 276.5 0 42 4 8 69.1 0 8 9 7 0.1 0 0 7 10 2 0.0 1 0 2 11 79 0.0 1 0 79 12 41 0.0 1 3 38 13 3 0.0 1 2 1 20 1 0.0 1 1 39 10 0.0 1 10 43 1 0.0 1 1 124 2 0.0 1 2 140 1 0.0 1 1 152 1 0.0 1 0 1 171 1 0.0 1 1