This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.75 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 143,310 (4.5%) Reads that were too short: 35,084 (1.1%) Reads written (passing filters): 108,226 (3.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 26,002,128 bp (2.7%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 143310 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 560 773.1 0 560 7 2930 773.1 0 2930 8 31551 773.1 0 31551 9 28182 773.1 0 28182 10 50 773.1 0 50 11 27 773.1 0 27 12 48 773.1 0 48 13 18 773.1 0 18 14 25 773.1 0 25 15 16 773.1 0 16 16 19 773.1 0 19 17 29 773.1 0 29 18 22 773.1 0 22 19 22 773.1 0 22 20 22 773.1 0 22 21 12 773.1 0 12 22 20 773.1 0 20 23 40 773.1 0 40 24 19 773.1 0 19 25 33 773.1 0 33 26 27 773.1 0 27 27 32 773.1 0 32 28 14 773.1 0 14 29 29 773.1 0 29 30 27 773.1 0 27 31 27 773.1 0 27 32 61 773.1 0 61 33 67 773.1 0 67 34 75 773.1 0 75 35 37 773.1 0 37 36 47 773.1 0 47 37 204 773.1 0 204 38 217 773.1 0 217 39 336 773.1 0 336 40 61 773.1 0 61 41 83 773.1 0 83 42 164 773.1 0 164 43 17 773.1 0 17 44 34 773.1 0 34 45 23 773.1 0 23 46 21 773.1 0 21 47 41 773.1 0 41 48 47 773.1 0 47 49 37 773.1 0 37 50 25 773.1 0 25 51 36 773.1 0 36 52 513 773.1 0 513 53 247 773.1 0 247 54 325 773.1 0 325 55 24 773.1 0 24 56 21 773.1 0 21 57 35 773.1 0 35 58 28 773.1 0 28 59 23 773.1 0 23 60 37 773.1 0 37 61 58 773.1 0 58 62 96 773.1 0 96 63 25 773.1 0 25 64 12 773.1 0 12 65 45 773.1 0 45 66 71 773.1 0 71 67 60 773.1 0 60 68 77 773.1 0 77 69 21 773.1 0 21 70 24 773.1 0 24 71 82 773.1 0 82 72 157 773.1 0 157 73 147 773.1 0 147 74 16 773.1 0 16 75 22 773.1 0 22 76 13 773.1 0 13 77 22 773.1 0 22 78 27 773.1 0 27 79 16 773.1 0 16 80 19 773.1 0 19 81 38 773.1 0 38 82 31 773.1 0 31 83 26 773.1 0 26 84 76 773.1 0 76 85 65 773.1 0 65 86 145 773.1 0 145 87 34 773.1 0 34 88 18 773.1 0 18 89 31 773.1 0 31 90 105 773.1 0 105 91 122 773.1 0 122 92 90 773.1 0 90 93 105 773.1 0 105 94 90 773.1 0 90 95 120 773.1 0 120 96 35 773.1 0 35 97 71 773.1 0 71 98 33 773.1 0 33 99 17 773.1 0 17 100 18 773.1 0 18 101 25 773.1 0 25 102 21 773.1 0 21 103 33 773.1 0 33 104 64 773.1 0 64 105 65 773.1 0 65 106 58 773.1 0 58 107 89 773.1 0 89 108 329 773.1 0 329 109 143 773.1 0 143 110 386 773.1 0 386 111 168 773.1 0 168 112 168 773.1 0 168 113 148 773.1 0 148 114 35 773.1 0 35 115 39 773.1 0 39 116 32 773.1 0 32 117 103 773.1 0 103 118 77 773.1 0 77 119 101 773.1 0 101 120 23 773.1 0 23 121 22 773.1 0 22 122 34 773.1 0 34 123 17 773.1 0 17 124 47 773.1 0 47 125 51 773.1 0 51 126 78 773.1 0 78 127 507 773.1 0 507 128 180 773.1 0 180 129 377 773.1 0 377 130 40 773.1 0 40 131 54 773.1 0 54 132 128 773.1 0 128 133 68 773.1 0 68 134 60 773.1 0 60 135 37 773.1 0 37 136 35 773.1 0 35 137 38 773.1 0 38 138 72 773.1 0 72 139 185 773.1 0 185 140 162 773.1 0 162 141 244 773.1 0 244 142 358 773.1 0 358 143 673 773.1 0 673 144 11053 773.1 0 11053 145 7922 773.1 0 7922 146 9545 773.1 0 9545 147 92 773.1 0 92 148 36 773.1 0 36 149 124 773.1 0 124 150 54 773.1 0 54 151 255 773.1 0 255 152 178 773.1 0 178 153 422 773.1 0 422 154 170 773.1 0 170 155 68 773.1 0 68 156 226 773.1 0 226 157 215 773.1 0 215 158 322 773.1 0 322 159 63 773.1 0 63 160 36 773.1 0 36 161 65 773.1 0 65 162 29 773.1 0 29 163 33 773.1 0 33 164 31 773.1 0 31 165 44 773.1 0 44 166 39 773.1 0 39 167 21 773.1 0 21 168 22 773.1 0 22 169 25 773.1 0 25 170 48 773.1 0 48 171 66 773.1 0 66 172 79 773.1 0 79 173 69 773.1 0 69 174 52 773.1 0 52 175 35 773.1 0 35 176 42 773.1 0 42 177 60 773.1 0 60 178 68 773.1 0 68 179 258 773.1 0 258 180 211 773.1 0 211 181 153 773.1 0 153 182 26 773.1 0 26 183 37 773.1 0 37 184 36 773.1 0 36 185 47 773.1 0 47 186 56 773.1 0 56 187 51 773.1 0 51 188 52 773.1 0 52 189 31 773.1 0 31 190 302 773.1 0 302 191 78 773.1 0 78 192 94 773.1 0 94 193 55 773.1 0 55 194 40 773.1 0 40 195 45 773.1 0 45 196 48 773.1 0 48 197 64 773.1 0 64 198 164 773.1 0 164 199 102 773.1 0 102 200 63 773.1 0 63 201 63 773.1 0 63 202 40 773.1 0 40 203 46 773.1 0 46 204 73 773.1 0 73 205 48 773.1 0 48 206 118 773.1 0 118 207 69 773.1 0 69 208 43 773.1 0 43 209 25 773.1 0 25 210 21 773.1 0 21 211 26 773.1 0 26 212 20 773.1 0 20 213 28 773.1 0 28 214 37 773.1 0 37 215 37 773.1 0 37 216 29 773.1 0 29 217 40 773.1 0 40 218 50 773.1 0 50 219 111 773.1 0 111 220 34 773.1 0 34 221 39 773.1 0 39 222 27 773.1 0 27 223 31 773.1 0 31 224 34 773.1 0 34 225 40 773.1 0 40 226 61 773.1 0 61 227 57 773.1 0 57 228 93 773.1 0 93 229 117 773.1 0 117 230 154 773.1 0 154 231 160 773.1 0 160 232 112 773.1 0 112 233 62 773.1 0 62 234 78 773.1 0 78 235 386 773.1 0 386 236 585 773.1 0 585 237 614 773.1 0 614 238 497 773.1 0 497 239 114 773.1 0 114 240 336 773.1 0 336 241 323 773.1 0 323 242 294 773.1 0 294 243 77 773.1 0 77 244 61 773.1 0 61 245 191 773.1 0 191 246 188 773.1 0 188 247 405 773.1 0 405 248 183 773.1 0 183 249 221 773.1 0 221 250 321 773.1 0 321 251 272 773.1 0 272 252 336 773.1 0 336 253 125 773.1 0 125 254 45 773.1 0 45 255 58 773.1 0 58 256 81 773.1 0 81 257 96 773.1 0 96 258 196 773.1 0 196 259 369 773.1 0 369 260 4511 773.1 0 4511 261 5560 773.1 0 5560 262 5711 773.1 0 5711 263 2609 773.1 0 2609 264 66 773.1 0 66 265 71 773.1 0 71 266 109 773.1 0 109 267 146 773.1 0 146 268 153 773.1 0 153 269 94 773.1 0 94 270 241 773.1 0 241 271 253 773.1 0 253 272 250 773.1 0 250 273 58 773.1 0 58 274 44 773.1 0 44 275 47 773.1 0 47 276 64 773.1 0 64 277 135 773.1 0 135 278 121 773.1 0 121 279 242 773.1 0 242 280 259 773.1 0 259 281 245 773.1 0 245 282 257 773.1 0 257 283 181 773.1 0 181 284 473 773.1 0 473 285 124 773.1 0 124 286 260 773.1 0 260 287 115 773.1 0 115 288 310 773.1 0 310 289 246 773.1 0 246 290 271 773.1 0 271 291 469 773.1 0 469 292 588 773.1 0 588 293 830 773.1 0 830 294 634 773.1 0 634 295 379 773.1 0 379 296 286 773.1 0 286 297 99 773.1 0 99 298 70 773.1 0 70 299 50 773.1 0 50 300 48 773.1 0 48 301 71 773.1 0 71 Finished in 7.01 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 108,226 Reads with adapters: 33,635 (31.1%) Reads that were too short: 89 (0.1%) Reads written (passing filters): 33,546 (31.0%) Total basepairs processed: 26,002,128 bp Total written (filtered): 9,251,676 bp (35.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33635 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 16 0.0 3 0 0 9 7 16 277 0.0 3 0 231 32 14 17 32914 0.0 3 31145 1441 269 59 18 79 0.0 3 0 61 11 7 19 94 0.0 3 9 3 48 34 20 96 0.0 3 1 0 0 95 21 2 0.0 3 0 0 1 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 2 0.0 3 0 0 0 2 29 3 0.0 3 0 0 0 3 31 3 0.0 3 0 0 0 3 34 1 0.0 3 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 1 121 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 7 0.0 3 0 0 0 7 152 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 201 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 1 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 217 31 0.0 3 0 0 1 30 218 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 281 3 0.0 3 0 0 1 2 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.77 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 33,546 Reads with adapters: 280 (0.8%) Reads written (passing filters): 33,546 (100.0%) Total basepairs processed: 9,251,676 bp Total written (filtered): 9,250,743 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 280 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.6% C: 10.4% G: 37.1% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 194 524.2 0 194 4 80 131.0 0 80 5 5 32.8 0 5 6 1 8.2 0 1