This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.85 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 128,613 (4.1%) Reads that were too short: 18,618 (0.6%) Reads written (passing filters): 109,995 (3.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 26,352,346 bp (2.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 128613 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 326 773.1 0 326 7 2077 773.1 0 2077 8 28893 773.1 0 28893 9 34176 773.1 0 34176 10 45 773.1 0 45 11 9 773.1 0 9 12 7 773.1 0 7 13 11 773.1 0 11 14 7 773.1 0 7 15 6 773.1 0 6 16 8 773.1 0 8 17 8 773.1 0 8 18 11 773.1 0 11 19 9 773.1 0 9 20 8 773.1 0 8 21 9 773.1 0 9 22 12 773.1 0 12 23 14 773.1 0 14 24 11 773.1 0 11 25 17 773.1 0 17 26 8 773.1 0 8 27 9 773.1 0 9 28 9 773.1 0 9 29 16 773.1 0 16 30 11 773.1 0 11 31 12 773.1 0 12 32 23 773.1 0 23 33 64 773.1 0 64 34 67 773.1 0 67 35 16 773.1 0 16 36 29 773.1 0 29 37 160 773.1 0 160 38 124 773.1 0 124 39 150 773.1 0 150 40 16 773.1 0 16 41 17 773.1 0 17 42 34 773.1 0 34 43 16 773.1 0 16 44 10 773.1 0 10 45 12 773.1 0 12 46 14 773.1 0 14 47 21 773.1 0 21 48 14 773.1 0 14 49 16 773.1 0 16 50 12 773.1 0 12 51 25 773.1 0 25 52 207 773.1 0 207 53 154 773.1 0 154 54 164 773.1 0 164 55 12 773.1 0 12 56 8 773.1 0 8 57 7 773.1 0 7 58 21 773.1 0 21 59 9 773.1 0 9 60 164 773.1 0 164 61 91 773.1 0 91 62 55 773.1 0 55 63 16 773.1 0 16 64 9 773.1 0 9 65 17 773.1 0 17 66 17 773.1 0 17 67 15 773.1 0 15 68 18 773.1 0 18 69 11 773.1 0 11 70 13 773.1 0 13 71 29 773.1 0 29 72 62 773.1 0 62 73 91 773.1 0 91 74 13 773.1 0 13 75 15 773.1 0 15 76 6 773.1 0 6 77 14 773.1 0 14 78 13 773.1 0 13 79 10 773.1 0 10 80 17 773.1 0 17 81 22 773.1 0 22 82 19 773.1 0 19 83 18 773.1 0 18 84 118 773.1 0 118 85 34 773.1 0 34 86 110 773.1 0 110 87 24 773.1 0 24 88 14 773.1 0 14 89 20 773.1 0 20 90 50 773.1 0 50 91 39 773.1 0 39 92 49 773.1 0 49 93 25 773.1 0 25 94 19 773.1 0 19 95 32 773.1 0 32 96 16 773.1 0 16 97 69 773.1 0 69 98 27 773.1 0 27 99 14 773.1 0 14 100 23 773.1 0 23 101 27 773.1 0 27 102 10 773.1 0 10 103 31 773.1 0 31 104 33 773.1 0 33 105 39 773.1 0 39 106 32 773.1 0 32 107 40 773.1 0 40 108 107 773.1 0 107 109 63 773.1 0 63 110 115 773.1 0 115 111 54 773.1 0 54 112 67 773.1 0 67 113 67 773.1 0 67 114 22 773.1 0 22 115 22 773.1 0 22 116 24 773.1 0 24 117 70 773.1 0 70 118 27 773.1 0 27 119 60 773.1 0 60 120 13 773.1 0 13 121 24 773.1 0 24 122 16 773.1 0 16 123 20 773.1 0 20 124 38 773.1 0 38 125 46 773.1 0 46 126 87 773.1 0 87 127 409 773.1 0 409 128 116 773.1 0 116 129 177 773.1 0 177 130 29 773.1 0 29 131 46 773.1 0 46 132 129 773.1 0 129 133 33 773.1 0 33 134 36 773.1 0 36 135 23 773.1 0 23 136 30 773.1 0 30 137 57 773.1 0 57 138 58 773.1 0 58 139 123 773.1 0 123 140 161 773.1 0 161 141 230 773.1 0 230 142 407 773.1 0 407 143 666 773.1 0 666 144 13590 773.1 0 13590 145 9937 773.1 0 9937 146 11364 773.1 0 11364 147 77 773.1 0 77 148 27 773.1 0 27 149 80 773.1 0 80 150 20 773.1 0 20 151 102 773.1 0 102 152 81 773.1 0 81 153 100 773.1 0 100 154 53 773.1 0 53 155 18 773.1 0 18 156 102 773.1 0 102 157 79 773.1 0 79 158 156 773.1 0 156 159 20 773.1 0 20 160 13 773.1 0 13 161 22 773.1 0 22 162 16 773.1 0 16 163 20 773.1 0 20 164 9 773.1 0 9 165 11 773.1 0 11 166 26 773.1 0 26 167 10 773.1 0 10 168 16 773.1 0 16 169 7 773.1 0 7 170 13 773.1 0 13 171 29 773.1 0 29 172 36 773.1 0 36 173 20 773.1 0 20 174 21 773.1 0 21 175 17 773.1 0 17 176 16 773.1 0 16 177 23 773.1 0 23 178 27 773.1 0 27 179 131 773.1 0 131 180 112 773.1 0 112 181 81 773.1 0 81 182 13 773.1 0 13 183 18 773.1 0 18 184 18 773.1 0 18 185 20 773.1 0 20 186 32 773.1 0 32 187 25 773.1 0 25 188 54 773.1 0 54 189 28 773.1 0 28 190 427 773.1 0 427 191 34 773.1 0 34 192 78 773.1 0 78 193 14 773.1 0 14 194 31 773.1 0 31 195 17 773.1 0 17 196 16 773.1 0 16 197 33 773.1 0 33 198 77 773.1 0 77 199 44 773.1 0 44 200 34 773.1 0 34 201 27 773.1 0 27 202 18 773.1 0 18 203 23 773.1 0 23 204 27 773.1 0 27 205 25 773.1 0 25 206 34 773.1 0 34 207 23 773.1 0 23 208 16 773.1 0 16 209 10 773.1 0 10 210 16 773.1 0 16 211 14 773.1 0 14 212 9 773.1 0 9 213 19 773.1 0 19 214 27 773.1 0 27 215 19 773.1 0 19 216 22 773.1 0 22 217 25 773.1 0 25 218 40 773.1 0 40 219 102 773.1 0 102 220 17 773.1 0 17 221 34 773.1 0 34 222 15 773.1 0 15 223 12 773.1 0 12 224 18 773.1 0 18 225 15 773.1 0 15 226 23 773.1 0 23 227 30 773.1 0 30 228 41 773.1 0 41 229 62 773.1 0 62 230 107 773.1 0 107 231 133 773.1 0 133 232 47 773.1 0 47 233 34 773.1 0 34 234 35 773.1 0 35 235 270 773.1 0 270 236 252 773.1 0 252 237 311 773.1 0 311 238 193 773.1 0 193 239 59 773.1 0 59 240 195 773.1 0 195 241 139 773.1 0 139 242 110 773.1 0 110 243 30 773.1 0 30 244 24 773.1 0 24 245 87 773.1 0 87 246 78 773.1 0 78 247 171 773.1 0 171 248 83 773.1 0 83 249 79 773.1 0 79 250 118 773.1 0 118 251 118 773.1 0 118 252 118 773.1 0 118 253 51 773.1 0 51 254 26 773.1 0 26 255 25 773.1 0 25 256 43 773.1 0 43 257 51 773.1 0 51 258 87 773.1 0 87 259 221 773.1 0 221 260 2442 773.1 0 2442 261 3543 773.1 0 3543 262 3327 773.1 0 3327 263 1521 773.1 0 1521 264 39 773.1 0 39 265 22 773.1 0 22 266 38 773.1 0 38 267 70 773.1 0 70 268 73 773.1 0 73 269 54 773.1 0 54 270 154 773.1 0 154 271 171 773.1 0 171 272 101 773.1 0 101 273 25 773.1 0 25 274 20 773.1 0 20 275 24 773.1 0 24 276 32 773.1 0 32 277 79 773.1 0 79 278 68 773.1 0 68 279 123 773.1 0 123 280 120 773.1 0 120 281 108 773.1 0 108 282 76 773.1 0 76 283 143 773.1 0 143 284 542 773.1 0 542 285 117 773.1 0 117 286 162 773.1 0 162 287 38 773.1 0 38 288 159 773.1 0 159 289 118 773.1 0 118 290 108 773.1 0 108 291 120 773.1 0 120 292 139 773.1 0 139 293 213 773.1 0 213 294 139 773.1 0 139 295 115 773.1 0 115 296 96 773.1 0 96 297 37 773.1 0 37 298 24 773.1 0 24 299 24 773.1 0 24 300 22 773.1 0 22 301 41 773.1 0 41 Finished in 7.49 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 109,995 Reads with adapters: 37,126 (33.8%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 37,117 (33.7%) Total basepairs processed: 26,352,346 bp Total written (filtered): 9,929,398 bp (37.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37126 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 12 0.0 3 0 0 11 1 19 378 0.0 3 0 372 4 0 2 20 35025 0.0 4 34482 518 20 3 2 21 85 0.0 4 7 74 4 22 2 0.0 4 1 0 1 23 20 0.0 4 0 0 0 19 1 32 1 0.0 4 1 44 1 0.0 4 1 95 1 0.0 4 0 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 18 0.0 4 0 0 0 0 18 127 1568 0.0 4 0 0 0 0 1568 128 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 1 153 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.01 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 37,117 Reads with adapters: 4,726 (12.7%) Reads written (passing filters): 37,117 (100.0%) Total basepairs processed: 9,929,398 bp Total written (filtered): 9,883,564 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4726 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.2% G: 96.1% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 580.0 0 9 4 8 145.0 0 8 5 1 36.2 0 1 9 2549 0.1 0 55 2494 10 1814 0.0 1 56 1758 11 332 0.0 1 1 331 12 7 0.0 1 1 6 13 1 0.0 1 0 1 150 1 0.0 1 0 1 170 1 0.0 1 1 172 1 0.0 1 1 190 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.