This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.11 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 94,955 (3.0%) Reads that were too short: 13,611 (0.4%) Reads written (passing filters): 81,344 (2.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,592,246 bp (2.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 94955 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 620 773.1 0 620 7 34797 773.1 0 34797 8 25586 773.1 0 25586 9 54 773.1 0 54 10 41 773.1 0 41 11 41 773.1 0 41 12 30 773.1 0 30 13 41 773.1 0 41 14 31 773.1 0 31 15 29 773.1 0 29 16 34 773.1 0 34 17 32 773.1 0 32 18 145 773.1 0 145 19 59 773.1 0 59 20 97 773.1 0 97 21 31 773.1 0 31 22 35 773.1 0 35 23 35 773.1 0 35 24 48 773.1 0 48 25 50 773.1 0 50 26 48 773.1 0 48 27 56 773.1 0 56 28 34 773.1 0 34 29 26 773.1 0 26 30 38 773.1 0 38 31 36 773.1 0 36 32 42 773.1 0 42 33 72 773.1 0 72 34 44 773.1 0 44 35 48 773.1 0 48 36 40 773.1 0 40 37 41 773.1 0 41 38 40 773.1 0 40 39 55 773.1 0 55 40 38 773.1 0 38 41 49 773.1 0 49 42 39 773.1 0 39 43 32 773.1 0 32 44 50 773.1 0 50 45 34 773.1 0 34 46 36 773.1 0 36 47 96 773.1 0 96 48 34 773.1 0 34 49 42 773.1 0 42 50 44 773.1 0 44 51 58 773.1 0 58 52 34 773.1 0 34 53 44 773.1 0 44 54 51 773.1 0 51 55 54 773.1 0 54 56 40 773.1 0 40 57 39 773.1 0 39 58 43 773.1 0 43 59 55 773.1 0 55 60 42 773.1 0 42 61 43 773.1 0 43 62 47 773.1 0 47 63 36 773.1 0 36 64 24 773.1 0 24 65 51 773.1 0 51 66 80 773.1 0 80 67 81 773.1 0 81 68 57 773.1 0 57 69 89 773.1 0 89 70 85 773.1 0 85 71 816 773.1 0 816 72 981 773.1 0 981 73 995 773.1 0 995 74 44 773.1 0 44 75 52 773.1 0 52 76 36 773.1 0 36 77 79 773.1 0 79 78 60 773.1 0 60 79 104 773.1 0 104 80 51 773.1 0 51 81 43 773.1 0 43 82 53 773.1 0 53 83 45 773.1 0 45 84 52 773.1 0 52 85 67 773.1 0 67 86 60 773.1 0 60 87 63 773.1 0 63 88 49 773.1 0 49 89 36 773.1 0 36 90 58 773.1 0 58 91 62 773.1 0 62 92 52 773.1 0 52 93 66 773.1 0 66 94 91 773.1 0 91 95 111 773.1 0 111 96 763 773.1 0 763 97 785 773.1 0 785 98 545 773.1 0 545 99 188 773.1 0 188 100 34 773.1 0 34 101 30 773.1 0 30 102 53 773.1 0 53 103 53 773.1 0 53 104 49 773.1 0 49 105 56 773.1 0 56 106 46 773.1 0 46 107 61 773.1 0 61 108 85 773.1 0 85 109 58 773.1 0 58 110 52 773.1 0 52 111 38 773.1 0 38 112 47 773.1 0 47 113 49 773.1 0 49 114 71 773.1 0 71 115 59 773.1 0 59 116 52 773.1 0 52 117 55 773.1 0 55 118 84 773.1 0 84 119 49 773.1 0 49 120 50 773.1 0 50 121 54 773.1 0 54 122 50 773.1 0 50 123 57 773.1 0 57 124 94 773.1 0 94 125 132 773.1 0 132 126 132 773.1 0 132 127 117 773.1 0 117 128 90 773.1 0 90 129 118 773.1 0 118 130 131 773.1 0 131 131 79 773.1 0 79 132 80 773.1 0 80 133 73 773.1 0 73 134 89 773.1 0 89 135 131 773.1 0 131 136 67 773.1 0 67 137 74 773.1 0 74 138 67 773.1 0 67 139 76 773.1 0 76 140 126 773.1 0 126 141 116 773.1 0 116 142 77 773.1 0 77 143 195 773.1 0 195 144 252 773.1 0 252 145 67 773.1 0 67 146 68 773.1 0 68 147 54 773.1 0 54 148 85 773.1 0 85 149 53 773.1 0 53 150 70 773.1 0 70 151 107 773.1 0 107 152 70 773.1 0 70 153 51 773.1 0 51 154 78 773.1 0 78 155 73 773.1 0 73 156 79 773.1 0 79 157 54 773.1 0 54 158 53 773.1 0 53 159 52 773.1 0 52 160 60 773.1 0 60 161 65 773.1 0 65 162 51 773.1 0 51 163 51 773.1 0 51 164 75 773.1 0 75 165 93 773.1 0 93 166 61 773.1 0 61 167 68 773.1 0 68 168 68 773.1 0 68 169 54 773.1 0 54 170 56 773.1 0 56 171 78 773.1 0 78 172 103 773.1 0 103 173 260 773.1 0 260 174 229 773.1 0 229 175 315 773.1 0 315 176 90 773.1 0 90 177 57 773.1 0 57 178 62 773.1 0 62 179 59 773.1 0 59 180 92 773.1 0 92 181 59 773.1 0 59 182 55 773.1 0 55 183 55 773.1 0 55 184 85 773.1 0 85 185 85 773.1 0 85 186 109 773.1 0 109 187 72 773.1 0 72 188 71 773.1 0 71 189 63 773.1 0 63 190 108 773.1 0 108 191 181 773.1 0 181 192 672 773.1 0 672 193 928 773.1 0 928 194 838 773.1 0 838 195 449 773.1 0 449 196 60 773.1 0 60 197 58 773.1 0 58 198 78 773.1 0 78 199 98 773.1 0 98 200 74 773.1 0 74 201 67 773.1 0 67 202 37 773.1 0 37 203 68 773.1 0 68 204 94 773.1 0 94 205 89 773.1 0 89 206 90 773.1 0 90 207 94 773.1 0 94 208 68 773.1 0 68 209 112 773.1 0 112 210 126 773.1 0 126 211 128 773.1 0 128 212 100 773.1 0 100 213 81 773.1 0 81 214 57 773.1 0 57 215 139 773.1 0 139 216 138 773.1 0 138 217 197 773.1 0 197 218 64 773.1 0 64 219 135 773.1 0 135 220 181 773.1 0 181 221 148 773.1 0 148 222 77 773.1 0 77 223 58 773.1 0 58 224 55 773.1 0 55 225 90 773.1 0 90 226 153 773.1 0 153 227 141 773.1 0 141 228 88 773.1 0 88 229 61 773.1 0 61 230 64 773.1 0 64 231 78 773.1 0 78 232 69 773.1 0 69 233 80 773.1 0 80 234 72 773.1 0 72 235 67 773.1 0 67 236 93 773.1 0 93 237 103 773.1 0 103 238 91 773.1 0 91 239 82 773.1 0 82 240 127 773.1 0 127 241 120 773.1 0 120 242 106 773.1 0 106 243 61 773.1 0 61 244 54 773.1 0 54 245 103 773.1 0 103 246 104 773.1 0 104 247 140 773.1 0 140 248 88 773.1 0 88 249 126 773.1 0 126 250 96 773.1 0 96 251 70 773.1 0 70 252 73 773.1 0 73 253 71 773.1 0 71 254 143 773.1 0 143 255 159 773.1 0 159 256 128 773.1 0 128 257 94 773.1 0 94 258 97 773.1 0 97 259 114 773.1 0 114 260 110 773.1 0 110 261 109 773.1 0 109 262 79 773.1 0 79 263 124 773.1 0 124 264 92 773.1 0 92 265 97 773.1 0 97 266 119 773.1 0 119 267 147 773.1 0 147 268 129 773.1 0 129 269 98 773.1 0 98 270 188 773.1 0 188 271 219 773.1 0 219 272 1065 773.1 0 1065 273 719 773.1 0 719 274 1110 773.1 0 1110 275 107 773.1 0 107 276 96 773.1 0 96 277 102 773.1 0 102 278 135 773.1 0 135 279 130 773.1 0 130 280 194 773.1 0 194 281 154 773.1 0 154 282 118 773.1 0 118 283 83 773.1 0 83 284 83 773.1 0 83 285 103 773.1 0 103 286 114 773.1 0 114 287 138 773.1 0 138 288 173 773.1 0 173 289 176 773.1 0 176 290 146 773.1 0 146 291 143 773.1 0 143 292 111 773.1 0 111 293 133 773.1 0 133 294 218 773.1 0 218 295 182 773.1 0 182 296 190 773.1 0 190 297 92 773.1 0 92 298 66 773.1 0 66 299 73 773.1 0 73 300 119 773.1 0 119 301 85 773.1 0 85 Finished in 5.56 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 81,344 Reads with adapters: 34,554 (42.5%) Reads that were too short: 45 (0.1%) Reads written (passing filters): 34,509 (42.4%) Total basepairs processed: 21,592,246 bp Total written (filtered): 9,544,832 bp (44.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34554 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 11 0.0 3 0 0 6 5 16 215 0.0 3 0 184 22 9 17 29782 0.0 3 28218 1302 213 49 18 144 0.0 3 3 128 6 7 19 4218 0.0 3 3954 176 61 27 20 85 0.0 3 4 5 1 75 21 11 0.0 3 0 0 9 2 22 4 0.0 3 0 0 0 4 24 1 0.0 3 0 0 1 25 3 0.0 3 0 0 0 3 26 1 0.0 3 0 0 0 1 28 3 0.0 3 0 0 1 2 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 138 2 0.0 3 0 1 0 1 139 2 0.0 3 1 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 215 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.87 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 34,509 Reads with adapters: 242 (0.7%) Reads written (passing filters): 34,509 (100.0%) Total basepairs processed: 9,544,832 bp Total written (filtered): 9,544,100 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 242 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.8% C: 28.1% G: 26.9% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 238 539.2 0 238 4 3 134.8 0 3 6 1 8.4 0 1