This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 138.42 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 87,664 (2.8%) Reads that were too short: 7,801 (0.2%) Reads written (passing filters): 79,863 (2.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,532,668 bp (2.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 87664 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 502 773.1 0 502 7 31134 773.1 0 31134 8 30440 773.1 0 30440 9 51 773.1 0 51 10 27 773.1 0 27 11 24 773.1 0 24 12 17 773.1 0 17 13 36 773.1 0 36 14 28 773.1 0 28 15 22 773.1 0 22 16 28 773.1 0 28 17 25 773.1 0 25 18 64 773.1 0 64 19 46 773.1 0 46 20 67 773.1 0 67 21 38 773.1 0 38 22 44 773.1 0 44 23 23 773.1 0 23 24 28 773.1 0 28 25 31 773.1 0 31 26 43 773.1 0 43 27 28 773.1 0 28 28 39 773.1 0 39 29 22 773.1 0 22 30 50 773.1 0 50 31 35 773.1 0 35 32 45 773.1 0 45 33 78 773.1 0 78 34 37 773.1 0 37 35 48 773.1 0 48 36 39 773.1 0 39 37 40 773.1 0 40 38 37 773.1 0 37 39 27 773.1 0 27 40 44 773.1 0 44 41 49 773.1 0 49 42 55 773.1 0 55 43 25 773.1 0 25 44 38 773.1 0 38 45 47 773.1 0 47 46 46 773.1 0 46 47 99 773.1 0 99 48 59 773.1 0 59 49 38 773.1 0 38 50 32 773.1 0 32 51 66 773.1 0 66 52 32 773.1 0 32 53 33 773.1 0 33 54 51 773.1 0 51 55 31 773.1 0 31 56 33 773.1 0 33 57 40 773.1 0 40 58 38 773.1 0 38 59 58 773.1 0 58 60 26 773.1 0 26 61 35 773.1 0 35 62 37 773.1 0 37 63 38 773.1 0 38 64 52 773.1 0 52 65 36 773.1 0 36 66 52 773.1 0 52 67 55 773.1 0 55 68 63 773.1 0 63 69 81 773.1 0 81 70 89 773.1 0 89 71 918 773.1 0 918 72 1017 773.1 0 1017 73 1110 773.1 0 1110 74 43 773.1 0 43 75 28 773.1 0 28 76 47 773.1 0 47 77 80 773.1 0 80 78 57 773.1 0 57 79 105 773.1 0 105 80 37 773.1 0 37 81 23 773.1 0 23 82 39 773.1 0 39 83 31 773.1 0 31 84 39 773.1 0 39 85 38 773.1 0 38 86 44 773.1 0 44 87 61 773.1 0 61 88 28 773.1 0 28 89 68 773.1 0 68 90 50 773.1 0 50 91 48 773.1 0 48 92 45 773.1 0 45 93 62 773.1 0 62 94 72 773.1 0 72 95 93 773.1 0 93 96 831 773.1 0 831 97 977 773.1 0 977 98 575 773.1 0 575 99 261 773.1 0 261 100 42 773.1 0 42 101 28 773.1 0 28 102 34 773.1 0 34 103 47 773.1 0 47 104 45 773.1 0 45 105 55 773.1 0 55 106 54 773.1 0 54 107 40 773.1 0 40 108 66 773.1 0 66 109 62 773.1 0 62 110 37 773.1 0 37 111 29 773.1 0 29 112 43 773.1 0 43 113 38 773.1 0 38 114 45 773.1 0 45 115 61 773.1 0 61 116 49 773.1 0 49 117 50 773.1 0 50 118 69 773.1 0 69 119 49 773.1 0 49 120 37 773.1 0 37 121 39 773.1 0 39 122 34 773.1 0 34 123 40 773.1 0 40 124 54 773.1 0 54 125 104 773.1 0 104 126 96 773.1 0 96 127 64 773.1 0 64 128 53 773.1 0 53 129 68 773.1 0 68 130 87 773.1 0 87 131 48 773.1 0 48 132 55 773.1 0 55 133 50 773.1 0 50 134 56 773.1 0 56 135 98 773.1 0 98 136 46 773.1 0 46 137 48 773.1 0 48 138 46 773.1 0 46 139 47 773.1 0 47 140 84 773.1 0 84 141 97 773.1 0 97 142 57 773.1 0 57 143 180 773.1 0 180 144 225 773.1 0 225 145 39 773.1 0 39 146 58 773.1 0 58 147 47 773.1 0 47 148 74 773.1 0 74 149 35 773.1 0 35 150 53 773.1 0 53 151 101 773.1 0 101 152 58 773.1 0 58 153 31 773.1 0 31 154 52 773.1 0 52 155 56 773.1 0 56 156 57 773.1 0 57 157 49 773.1 0 49 158 46 773.1 0 46 159 45 773.1 0 45 160 56 773.1 0 56 161 43 773.1 0 43 162 34 773.1 0 34 163 37 773.1 0 37 164 44 773.1 0 44 165 64 773.1 0 64 166 39 773.1 0 39 167 48 773.1 0 48 168 41 773.1 0 41 169 43 773.1 0 43 170 49 773.1 0 49 171 49 773.1 0 49 172 76 773.1 0 76 173 140 773.1 0 140 174 114 773.1 0 114 175 172 773.1 0 172 176 37 773.1 0 37 177 52 773.1 0 52 178 52 773.1 0 52 179 58 773.1 0 58 180 48 773.1 0 48 181 33 773.1 0 33 182 37 773.1 0 37 183 38 773.1 0 38 184 32 773.1 0 32 185 63 773.1 0 63 186 47 773.1 0 47 187 53 773.1 0 53 188 38 773.1 0 38 189 52 773.1 0 52 190 77 773.1 0 77 191 107 773.1 0 107 192 669 773.1 0 669 193 903 773.1 0 903 194 672 773.1 0 672 195 347 773.1 0 347 196 50 773.1 0 50 197 46 773.1 0 46 198 47 773.1 0 47 199 58 773.1 0 58 200 49 773.1 0 49 201 44 773.1 0 44 202 26 773.1 0 26 203 47 773.1 0 47 204 73 773.1 0 73 205 60 773.1 0 60 206 50 773.1 0 50 207 75 773.1 0 75 208 48 773.1 0 48 209 38 773.1 0 38 210 38 773.1 0 38 211 58 773.1 0 58 212 45 773.1 0 45 213 90 773.1 0 90 214 52 773.1 0 52 215 87 773.1 0 87 216 62 773.1 0 62 217 85 773.1 0 85 218 47 773.1 0 47 219 56 773.1 0 56 220 81 773.1 0 81 221 59 773.1 0 59 222 50 773.1 0 50 223 41 773.1 0 41 224 38 773.1 0 38 225 88 773.1 0 88 226 114 773.1 0 114 227 73 773.1 0 73 228 46 773.1 0 46 229 73 773.1 0 73 230 46 773.1 0 46 231 32 773.1 0 32 232 35 773.1 0 35 233 47 773.1 0 47 234 47 773.1 0 47 235 44 773.1 0 44 236 69 773.1 0 69 237 54 773.1 0 54 238 43 773.1 0 43 239 54 773.1 0 54 240 71 773.1 0 71 241 59 773.1 0 59 242 54 773.1 0 54 243 34 773.1 0 34 244 32 773.1 0 32 245 54 773.1 0 54 246 61 773.1 0 61 247 71 773.1 0 71 248 52 773.1 0 52 249 65 773.1 0 65 250 47 773.1 0 47 251 44 773.1 0 44 252 39 773.1 0 39 253 52 773.1 0 52 254 74 773.1 0 74 255 81 773.1 0 81 256 67 773.1 0 67 257 74 773.1 0 74 258 61 773.1 0 61 259 64 773.1 0 64 260 59 773.1 0 59 261 46 773.1 0 46 262 70 773.1 0 70 263 77 773.1 0 77 264 54 773.1 0 54 265 84 773.1 0 84 266 77 773.1 0 77 267 72 773.1 0 72 268 79 773.1 0 79 269 57 773.1 0 57 270 165 773.1 0 165 271 225 773.1 0 225 272 580 773.1 0 580 273 342 773.1 0 342 274 487 773.1 0 487 275 59 773.1 0 59 276 50 773.1 0 50 277 49 773.1 0 49 278 81 773.1 0 81 279 90 773.1 0 90 280 136 773.1 0 136 281 94 773.1 0 94 282 65 773.1 0 65 283 48 773.1 0 48 284 59 773.1 0 59 285 63 773.1 0 63 286 71 773.1 0 71 287 74 773.1 0 74 288 88 773.1 0 88 289 88 773.1 0 88 290 75 773.1 0 75 291 110 773.1 0 110 292 51 773.1 0 51 293 61 773.1 0 61 294 85 773.1 0 85 295 80 773.1 0 80 296 83 773.1 0 83 297 59 773.1 0 59 298 60 773.1 0 60 299 58 773.1 0 58 300 70 773.1 0 70 301 93 773.1 0 93 Finished in 5.78 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 79,863 Reads with adapters: 32,524 (40.7%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 32,515 (40.7%) Total basepairs processed: 21,532,668 bp Total written (filtered): 8,735,070 bp (40.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32524 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 14 0.0 3 0 0 14 19 450 0.0 3 0 434 15 1 20 30598 0.0 4 30076 501 20 1 21 74 0.0 4 13 59 1 1 22 9 0.0 4 4 0 4 0 1 23 18 0.0 4 0 0 0 17 1 24 4 0.0 4 2 0 0 0 2 35 1 0.0 4 1 66 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 1326 0.0 4 0 0 0 0 1326 128 3 0.0 4 0 0 0 0 3 129 6 0.0 4 0 0 0 0 6 137 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 1 1 141 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.75 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 32,515 Reads with adapters: 2,431 (7.5%) Reads written (passing filters): 32,515 (100.0%) Total basepairs processed: 8,735,070 bp Total written (filtered): 8,707,824 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2431 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.3% G: 94.8% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 508.0 0 24 4 4 127.0 0 4 5 1 31.8 0 1 9 25 0.1 0 0 25 10 81 0.0 1 4 77 11 2287 0.0 1 10 2277 12 2 0.0 1 0 2 13 1 0.0 1 0 1 14 1 0.0 1 0 1 151 2 0.0 1 0 2 153 1 0.0 1 0 1 224 1 0.0 1 1 231 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.