This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.40 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 91,222 (2.9%) Reads that were too short: 4,645 (0.1%) Reads written (passing filters): 86,577 (2.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 22,659,397 bp (2.4%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 91222 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1059 773.1 0 1059 7 30885 773.1 0 30885 8 416 773.1 0 416 9 24036 773.1 0 24036 10 50 773.1 0 50 11 196 773.1 0 196 12 45 773.1 0 45 13 90 773.1 0 90 14 22 773.1 0 22 15 30 773.1 0 30 16 81 773.1 0 81 17 62 773.1 0 62 18 77 773.1 0 77 19 49 773.1 0 49 20 35 773.1 0 35 21 35 773.1 0 35 22 38 773.1 0 38 23 44 773.1 0 44 24 28 773.1 0 28 25 36 773.1 0 36 26 49 773.1 0 49 27 30 773.1 0 30 28 38 773.1 0 38 29 46 773.1 0 46 30 44 773.1 0 44 31 38 773.1 0 38 32 71 773.1 0 71 33 39 773.1 0 39 34 51 773.1 0 51 35 34 773.1 0 34 36 46 773.1 0 46 37 61 773.1 0 61 38 39 773.1 0 39 39 33 773.1 0 33 40 31 773.1 0 31 41 38 773.1 0 38 42 56 773.1 0 56 43 46 773.1 0 46 44 44 773.1 0 44 45 57 773.1 0 57 46 356 773.1 0 356 47 475 773.1 0 475 48 283 773.1 0 283 49 34 773.1 0 34 50 23 773.1 0 23 51 49 773.1 0 49 52 35 773.1 0 35 53 22 773.1 0 22 54 32 773.1 0 32 55 31 773.1 0 31 56 31 773.1 0 31 57 44 773.1 0 44 58 49 773.1 0 49 59 57 773.1 0 57 60 40 773.1 0 40 61 43 773.1 0 43 62 34 773.1 0 34 63 45 773.1 0 45 64 40 773.1 0 40 65 42 773.1 0 42 66 50 773.1 0 50 67 60 773.1 0 60 68 34 773.1 0 34 69 53 773.1 0 53 70 48 773.1 0 48 71 39 773.1 0 39 72 43 773.1 0 43 73 37 773.1 0 37 74 42 773.1 0 42 75 34 773.1 0 34 76 50 773.1 0 50 77 39 773.1 0 39 78 52 773.1 0 52 79 207 773.1 0 207 80 169 773.1 0 169 81 318 773.1 0 318 82 95 773.1 0 95 83 112 773.1 0 112 84 89 773.1 0 89 85 72 773.1 0 72 86 50 773.1 0 50 87 64 773.1 0 64 88 82 773.1 0 82 89 91 773.1 0 91 90 76 773.1 0 76 91 92 773.1 0 92 92 157 773.1 0 157 93 202 773.1 0 202 94 2870 773.1 0 2870 95 2279 773.1 0 2279 96 6930 773.1 0 6930 97 2197 773.1 0 2197 98 3249 773.1 0 3249 99 38 773.1 0 38 100 27 773.1 0 27 101 43 773.1 0 43 102 35 773.1 0 35 103 33 773.1 0 33 104 41 773.1 0 41 105 46 773.1 0 46 106 39 773.1 0 39 107 49 773.1 0 49 108 106 773.1 0 106 109 36 773.1 0 36 110 139 773.1 0 139 111 71 773.1 0 71 112 52 773.1 0 52 113 40 773.1 0 40 114 35 773.1 0 35 115 42 773.1 0 42 116 38 773.1 0 38 117 36 773.1 0 36 118 130 773.1 0 130 119 43 773.1 0 43 120 44 773.1 0 44 121 35 773.1 0 35 122 34 773.1 0 34 123 41 773.1 0 41 124 270 773.1 0 270 125 171 773.1 0 171 126 183 773.1 0 183 127 34 773.1 0 34 128 45 773.1 0 45 129 52 773.1 0 52 130 139 773.1 0 139 131 257 773.1 0 257 132 206 773.1 0 206 133 260 773.1 0 260 134 125 773.1 0 125 135 53 773.1 0 53 136 58 773.1 0 58 137 104 773.1 0 104 138 151 773.1 0 151 139 352 773.1 0 352 140 54 773.1 0 54 141 94 773.1 0 94 142 82 773.1 0 82 143 130 773.1 0 130 144 86 773.1 0 86 145 71 773.1 0 71 146 76 773.1 0 76 147 55 773.1 0 55 148 59 773.1 0 59 149 92 773.1 0 92 150 27 773.1 0 27 151 35 773.1 0 35 152 23 773.1 0 23 153 17 773.1 0 17 154 41 773.1 0 41 155 26 773.1 0 26 156 28 773.1 0 28 157 47 773.1 0 47 158 322 773.1 0 322 159 188 773.1 0 188 160 274 773.1 0 274 161 23 773.1 0 23 162 8 773.1 0 8 163 18 773.1 0 18 164 19 773.1 0 19 165 19 773.1 0 19 166 24 773.1 0 24 167 29 773.1 0 29 168 17 773.1 0 17 169 26 773.1 0 26 170 20 773.1 0 20 171 18 773.1 0 18 172 19 773.1 0 19 173 12 773.1 0 12 174 17 773.1 0 17 175 13 773.1 0 13 176 39 773.1 0 39 177 14 773.1 0 14 178 24 773.1 0 24 179 67 773.1 0 67 180 23 773.1 0 23 181 27 773.1 0 27 182 28 773.1 0 28 183 30 773.1 0 30 184 55 773.1 0 55 185 34 773.1 0 34 186 46 773.1 0 46 187 44 773.1 0 44 188 31 773.1 0 31 189 14 773.1 0 14 190 17 773.1 0 17 191 16 773.1 0 16 192 58 773.1 0 58 193 42 773.1 0 42 194 45 773.1 0 45 195 16 773.1 0 16 196 26 773.1 0 26 197 17 773.1 0 17 198 27 773.1 0 27 199 22 773.1 0 22 200 12 773.1 0 12 201 9 773.1 0 9 202 28 773.1 0 28 203 42 773.1 0 42 204 32 773.1 0 32 205 321 773.1 0 321 206 497 773.1 0 497 207 724 773.1 0 724 208 24 773.1 0 24 209 23 773.1 0 23 210 24 773.1 0 24 211 20 773.1 0 20 212 13 773.1 0 13 213 19 773.1 0 19 214 16 773.1 0 16 215 13 773.1 0 13 216 15 773.1 0 15 217 18 773.1 0 18 218 34 773.1 0 34 219 15 773.1 0 15 220 20 773.1 0 20 221 26 773.1 0 26 222 23 773.1 0 23 223 50 773.1 0 50 224 10 773.1 0 10 225 18 773.1 0 18 226 20 773.1 0 20 227 33 773.1 0 33 228 29 773.1 0 29 229 22 773.1 0 22 230 14 773.1 0 14 231 14 773.1 0 14 232 32 773.1 0 32 233 28 773.1 0 28 234 17 773.1 0 17 235 27 773.1 0 27 236 21 773.1 0 21 237 23 773.1 0 23 238 25 773.1 0 25 239 21 773.1 0 21 240 24 773.1 0 24 241 30 773.1 0 30 242 25 773.1 0 25 243 19 773.1 0 19 244 18 773.1 0 18 245 26 773.1 0 26 246 37 773.1 0 37 247 22 773.1 0 22 248 24 773.1 0 24 249 20 773.1 0 20 250 25 773.1 0 25 251 21 773.1 0 21 252 60 773.1 0 60 253 43 773.1 0 43 254 73 773.1 0 73 255 21 773.1 0 21 256 30 773.1 0 30 257 41 773.1 0 41 258 25 773.1 0 25 259 34 773.1 0 34 260 12 773.1 0 12 261 37 773.1 0 37 262 36 773.1 0 36 263 45 773.1 0 45 264 41 773.1 0 41 265 22 773.1 0 22 266 45 773.1 0 45 267 18 773.1 0 18 268 33 773.1 0 33 269 18 773.1 0 18 270 44 773.1 0 44 271 55 773.1 0 55 272 62 773.1 0 62 273 39 773.1 0 39 274 20 773.1 0 20 275 34 773.1 0 34 276 40 773.1 0 40 277 45 773.1 0 45 278 31 773.1 0 31 279 44 773.1 0 44 280 22 773.1 0 22 281 54 773.1 0 54 282 71 773.1 0 71 283 85 773.1 0 85 284 58 773.1 0 58 285 33 773.1 0 33 286 52 773.1 0 52 287 31 773.1 0 31 288 55 773.1 0 55 289 53 773.1 0 53 290 42 773.1 0 42 291 30 773.1 0 30 292 32 773.1 0 32 293 19 773.1 0 19 294 26 773.1 0 26 295 39 773.1 0 39 296 38 773.1 0 38 297 32 773.1 0 32 298 35 773.1 0 35 299 42 773.1 0 42 300 62 773.1 0 62 301 44 773.1 0 44 Finished in 6.15 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 86,577 Reads with adapters: 32,299 (37.3%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 32,260 (37.3%) Total basepairs processed: 22,659,397 bp Total written (filtered): 8,671,365 bp (38.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32299 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 254 0.0 3 0 248 3 2 1 20 30370 0.0 4 29836 519 12 2 1 21 81 0.0 4 14 64 3 22 6 0.0 4 3 0 2 1 23 20 0.0 4 0 0 0 16 4 24 5 0.0 4 3 0 0 1 1 25 1 0.0 4 0 1 56 1 0.0 4 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 117 3 0.0 4 0 0 0 0 3 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 13 0.0 4 0 0 0 0 13 127 1113 0.0 4 0 0 0 0 1113 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 52 0.0 4 0 0 0 0 52 134 3 0.0 4 0 0 0 0 3 135 327 0.0 4 0 0 0 2 325 141 18 0.0 4 0 0 0 0 18 142 6 0.0 4 0 0 0 2 4 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 0 0 0 2 159 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 200 1 0.0 4 1 227 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 32,260 Reads with adapters: 399 (1.2%) Reads written (passing filters): 32,260 (100.0%) Total basepairs processed: 8,671,365 bp Total written (filtered): 8,667,098 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 399 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.0% C: 8.0% G: 76.7% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 65 504.1 0 65 4 11 126.0 0 11 5 1 31.5 0 1 9 7 0.1 0 1 6 10 7 0.0 1 0 7 11 247 0.0 1 0 247 12 56 0.0 1 2 54 13 2 0.0 1 0 2 152 2 0.0 1 0 2 171 1 0.0 1 1