This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.87 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 234,868 (7.4%) Reads that were too short: 31,364 (1.0%) Reads written (passing filters): 203,504 (6.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,253,403 bp (3.9%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 234868 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 210 773.1 0 210 7 4233 773.1 0 4233 8 161 773.1 0 161 9 3874 773.1 0 3874 10 29 773.1 0 29 11 28 773.1 0 28 12 35 773.1 0 35 13 38 773.1 0 38 14 78 773.1 0 78 15 69 773.1 0 69 16 78 773.1 0 78 17 32 773.1 0 32 18 8 773.1 0 8 19 29 773.1 0 29 20 27 773.1 0 27 21 39 773.1 0 39 22 43 773.1 0 43 23 28 773.1 0 28 24 74 773.1 0 74 25 43 773.1 0 43 26 28 773.1 0 28 27 39 773.1 0 39 28 36 773.1 0 36 29 43 773.1 0 43 30 36 773.1 0 36 31 39 773.1 0 39 32 57 773.1 0 57 33 49 773.1 0 49 34 50 773.1 0 50 35 52 773.1 0 52 36 42 773.1 0 42 37 50 773.1 0 50 38 46 773.1 0 46 39 85 773.1 0 85 40 64 773.1 0 64 41 65 773.1 0 65 42 34 773.1 0 34 43 44 773.1 0 44 44 25 773.1 0 25 45 52 773.1 0 52 46 70 773.1 0 70 47 42 773.1 0 42 48 49 773.1 0 49 49 27 773.1 0 27 50 49 773.1 0 49 51 57 773.1 0 57 52 77 773.1 0 77 53 55 773.1 0 55 54 48 773.1 0 48 55 76 773.1 0 76 56 208 773.1 0 208 57 196 773.1 0 196 58 279 773.1 0 279 59 495 773.1 0 495 60 168 773.1 0 168 61 269 773.1 0 269 62 40 773.1 0 40 63 54 773.1 0 54 64 56 773.1 0 56 65 54 773.1 0 54 66 40 773.1 0 40 67 60 773.1 0 60 68 37 773.1 0 37 69 82 773.1 0 82 70 68 773.1 0 68 71 41 773.1 0 41 72 71 773.1 0 71 73 60 773.1 0 60 74 74 773.1 0 74 75 41 773.1 0 41 76 41 773.1 0 41 77 197 773.1 0 197 78 60 773.1 0 60 79 49 773.1 0 49 80 46 773.1 0 46 81 65 773.1 0 65 82 50 773.1 0 50 83 97 773.1 0 97 84 97 773.1 0 97 85 101 773.1 0 101 86 69 773.1 0 69 87 588 773.1 0 588 88 54 773.1 0 54 89 120 773.1 0 120 90 85 773.1 0 85 91 120 773.1 0 120 92 120 773.1 0 120 93 64 773.1 0 64 94 100 773.1 0 100 95 86 773.1 0 86 96 121 773.1 0 121 97 234 773.1 0 234 98 89 773.1 0 89 99 151 773.1 0 151 100 143 773.1 0 143 101 168 773.1 0 168 102 412 773.1 0 412 103 226 773.1 0 226 104 94 773.1 0 94 105 81 773.1 0 81 106 555 773.1 0 555 107 495 773.1 0 495 108 572 773.1 0 572 109 393 773.1 0 393 110 519 773.1 0 519 111 577 773.1 0 577 112 1253 773.1 0 1253 113 2651 773.1 0 2651 114 40368 773.1 0 40368 115 26370 773.1 0 26370 116 62120 773.1 0 62120 117 449 773.1 0 449 118 192 773.1 0 192 119 35 773.1 0 35 120 48 773.1 0 48 121 37 773.1 0 37 122 31 773.1 0 31 123 38 773.1 0 38 124 55 773.1 0 55 125 49 773.1 0 49 126 98 773.1 0 98 127 74 773.1 0 74 128 183 773.1 0 183 129 184 773.1 0 184 130 133 773.1 0 133 131 137 773.1 0 137 132 324 773.1 0 324 133 70 773.1 0 70 134 57 773.1 0 57 135 83 773.1 0 83 136 87 773.1 0 87 137 131 773.1 0 131 138 88 773.1 0 88 139 54 773.1 0 54 140 404 773.1 0 404 141 817 773.1 0 817 142 659 773.1 0 659 143 288 773.1 0 288 144 189 773.1 0 189 145 278 773.1 0 278 146 440 773.1 0 440 147 9797 773.1 0 9797 148 4308 773.1 0 4308 149 8655 773.1 0 8655 150 252 773.1 0 252 151 213 773.1 0 213 152 384 773.1 0 384 153 5202 773.1 0 5202 154 3417 773.1 0 3417 155 4829 773.1 0 4829 156 461 773.1 0 461 157 377 773.1 0 377 158 251 773.1 0 251 159 49 773.1 0 49 160 86 773.1 0 86 161 122 773.1 0 122 162 153 773.1 0 153 163 165 773.1 0 165 164 111 773.1 0 111 165 103 773.1 0 103 166 65 773.1 0 65 167 120 773.1 0 120 168 571 773.1 0 571 169 364 773.1 0 364 170 372 773.1 0 372 171 163 773.1 0 163 172 54 773.1 0 54 173 41 773.1 0 41 174 35 773.1 0 35 175 96 773.1 0 96 176 44 773.1 0 44 177 46 773.1 0 46 178 66 773.1 0 66 179 50 773.1 0 50 180 59 773.1 0 59 181 98 773.1 0 98 182 127 773.1 0 127 183 525 773.1 0 525 184 345 773.1 0 345 185 671 773.1 0 671 186 475 773.1 0 475 187 276 773.1 0 276 188 116 773.1 0 116 189 110 773.1 0 110 190 135 773.1 0 135 191 357 773.1 0 357 192 93 773.1 0 93 193 42 773.1 0 42 194 48 773.1 0 48 195 70 773.1 0 70 196 39 773.1 0 39 197 46 773.1 0 46 198 134 773.1 0 134 199 40 773.1 0 40 200 69 773.1 0 69 201 59 773.1 0 59 202 172 773.1 0 172 203 133 773.1 0 133 204 164 773.1 0 164 205 51 773.1 0 51 206 46 773.1 0 46 207 31 773.1 0 31 208 27 773.1 0 27 209 42 773.1 0 42 210 38 773.1 0 38 211 39 773.1 0 39 212 42 773.1 0 42 213 56 773.1 0 56 214 51 773.1 0 51 215 92 773.1 0 92 216 349 773.1 0 349 217 295 773.1 0 295 218 254 773.1 0 254 219 42 773.1 0 42 220 39 773.1 0 39 221 43 773.1 0 43 222 62 773.1 0 62 223 50 773.1 0 50 224 48 773.1 0 48 225 43 773.1 0 43 226 107 773.1 0 107 227 79 773.1 0 79 228 56 773.1 0 56 229 35 773.1 0 35 230 71 773.1 0 71 231 70 773.1 0 70 232 72 773.1 0 72 233 124 773.1 0 124 234 147 773.1 0 147 235 271 773.1 0 271 236 158 773.1 0 158 237 125 773.1 0 125 238 68 773.1 0 68 239 62 773.1 0 62 240 62 773.1 0 62 241 37 773.1 0 37 242 73 773.1 0 73 243 54 773.1 0 54 244 75 773.1 0 75 245 91 773.1 0 91 246 159 773.1 0 159 247 73 773.1 0 73 248 78 773.1 0 78 249 155 773.1 0 155 250 125 773.1 0 125 251 166 773.1 0 166 252 66 773.1 0 66 253 92 773.1 0 92 254 150 773.1 0 150 255 117 773.1 0 117 256 71 773.1 0 71 257 65 773.1 0 65 258 61 773.1 0 61 259 65 773.1 0 65 260 75 773.1 0 75 261 68 773.1 0 68 262 94 773.1 0 94 263 72 773.1 0 72 264 85 773.1 0 85 265 115 773.1 0 115 266 89 773.1 0 89 267 150 773.1 0 150 268 159 773.1 0 159 269 116 773.1 0 116 270 273 773.1 0 273 271 290 773.1 0 290 272 223 773.1 0 223 273 142 773.1 0 142 274 125 773.1 0 125 275 184 773.1 0 184 276 184 773.1 0 184 277 160 773.1 0 160 278 267 773.1 0 267 279 848 773.1 0 848 280 1361 773.1 0 1361 281 5503 773.1 0 5503 282 4380 773.1 0 4380 283 5944 773.1 0 5944 284 214 773.1 0 214 285 238 773.1 0 238 286 158 773.1 0 158 287 101 773.1 0 101 288 91 773.1 0 91 289 136 773.1 0 136 290 542 773.1 0 542 291 524 773.1 0 524 292 567 773.1 0 567 293 438 773.1 0 438 294 271 773.1 0 271 295 358 773.1 0 358 296 344 773.1 0 344 297 339 773.1 0 339 298 366 773.1 0 366 299 139 773.1 0 139 300 63 773.1 0 63 301 79 773.1 0 79 Finished in 10.51 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 203,504 Reads with adapters: 4,583 (2.3%) Reads that were too short: 148 (0.1%) Reads written (passing filters): 4,435 (2.2%) Total basepairs processed: 37,253,403 bp Total written (filtered): 1,196,577 bp (3.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4583 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 46 0.0 3 0 39 6 1 17 4133 0.0 3 3898 187 39 9 18 27 0.0 3 12 14 0 1 19 13 0.0 3 2 3 6 2 20 16 0.0 3 3 4 2 7 22 1 0.0 3 0 0 1 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 5 0.0 3 0 0 0 5 33 5 0.0 3 0 0 0 5 34 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 0 4 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 6 0.0 3 2 0 0 4 49 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 51 51 0.0 3 0 0 0 51 53 1 0.0 3 0 0 0 1 54 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 57 4 0.0 3 0 0 0 4 58 3 0.0 3 0 0 0 3 59 6 0.0 3 0 0 0 6 60 6 0.0 3 0 0 0 6 61 3 0.0 3 0 0 0 3 63 4 0.0 3 0 0 1 3 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 82 9 0.0 3 0 0 0 9 84 2 0.0 3 0 0 0 2 85 47 0.0 3 0 0 0 47 86 10 0.0 3 0 0 0 10 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 5 0.0 3 0 0 0 5 104 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 10 0.0 3 0 0 0 10 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 4 0.0 3 0 0 1 3 135 2 0.0 3 0 0 0 2 136 13 0.0 3 0 0 0 13 137 1 0.0 3 0 0 0 1 138 4 0.0 3 0 0 0 4 139 1 0.0 3 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 1 152 5 0.0 3 0 0 0 5 153 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 11 0.0 3 0 0 0 11 173 1 0.0 3 0 0 0 1 174 14 0.0 3 0 0 1 13 175 11 0.0 3 0 0 1 10 178 4 0.0 3 0 0 0 4 183 1 0.0 3 0 0 1 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 4,435 Reads with adapters: 23 (0.5%) Reads written (passing filters): 4,435 (100.0%) Total basepairs processed: 1,196,577 bp Total written (filtered): 1,196,503 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 23 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.4% C: 60.9% G: 17.4% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19 69.3 0 19 4 3 17.3 0 3 5 1 4.3 0 1