This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.02 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 288,525 (9.1%) Reads that were too short: 14,987 (0.5%) Reads written (passing filters): 273,538 (8.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 50,184,484 bp (5.3%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 288525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 141 773.1 0 141 7 4038 773.1 0 4038 8 148 773.1 0 148 9 4282 773.1 0 4282 10 18 773.1 0 18 11 18 773.1 0 18 12 19 773.1 0 19 13 33 773.1 0 33 14 19 773.1 0 19 15 35 773.1 0 35 16 33 773.1 0 33 17 28 773.1 0 28 18 17 773.1 0 17 19 29 773.1 0 29 20 29 773.1 0 29 21 45 773.1 0 45 22 26 773.1 0 26 23 36 773.1 0 36 24 30 773.1 0 30 25 38 773.1 0 38 26 42 773.1 0 42 27 44 773.1 0 44 28 30 773.1 0 30 29 22 773.1 0 22 30 31 773.1 0 31 31 44 773.1 0 44 32 42 773.1 0 42 33 53 773.1 0 53 34 34 773.1 0 34 35 42 773.1 0 42 36 30 773.1 0 30 37 49 773.1 0 49 38 34 773.1 0 34 39 76 773.1 0 76 40 46 773.1 0 46 41 31 773.1 0 31 42 34 773.1 0 34 43 30 773.1 0 30 44 29 773.1 0 29 45 38 773.1 0 38 46 48 773.1 0 48 47 40 773.1 0 40 48 43 773.1 0 43 49 36 773.1 0 36 50 32 773.1 0 32 51 56 773.1 0 56 52 77 773.1 0 77 53 47 773.1 0 47 54 62 773.1 0 62 55 65 773.1 0 65 56 117 773.1 0 117 57 93 773.1 0 93 58 105 773.1 0 105 59 1146 773.1 0 1146 60 227 773.1 0 227 61 236 773.1 0 236 62 52 773.1 0 52 63 28 773.1 0 28 64 53 773.1 0 53 65 48 773.1 0 48 66 25 773.1 0 25 67 52 773.1 0 52 68 50 773.1 0 50 69 80 773.1 0 80 70 76 773.1 0 76 71 43 773.1 0 43 72 64 773.1 0 64 73 38 773.1 0 38 74 68 773.1 0 68 75 61 773.1 0 61 76 61 773.1 0 61 77 270 773.1 0 270 78 58 773.1 0 58 79 49 773.1 0 49 80 47 773.1 0 47 81 51 773.1 0 51 82 43 773.1 0 43 83 63 773.1 0 63 84 81 773.1 0 81 85 67 773.1 0 67 86 83 773.1 0 83 87 647 773.1 0 647 88 58 773.1 0 58 89 64 773.1 0 64 90 62 773.1 0 62 91 53 773.1 0 53 92 107 773.1 0 107 93 59 773.1 0 59 94 82 773.1 0 82 95 105 773.1 0 105 96 157 773.1 0 157 97 340 773.1 0 340 98 104 773.1 0 104 99 168 773.1 0 168 100 160 773.1 0 160 101 205 773.1 0 205 102 643 773.1 0 643 103 252 773.1 0 252 104 127 773.1 0 127 105 78 773.1 0 78 106 295 773.1 0 295 107 376 773.1 0 376 108 412 773.1 0 412 109 466 773.1 0 466 110 703 773.1 0 703 111 725 773.1 0 725 112 1723 773.1 0 1723 113 3400 773.1 0 3400 114 61884 773.1 0 61884 115 37977 773.1 0 37977 116 93208 773.1 0 93208 117 479 773.1 0 479 118 81 773.1 0 81 119 41 773.1 0 41 120 60 773.1 0 60 121 36 773.1 0 36 122 19 773.1 0 19 123 45 773.1 0 45 124 68 773.1 0 68 125 47 773.1 0 47 126 91 773.1 0 91 127 88 773.1 0 88 128 184 773.1 0 184 129 148 773.1 0 148 130 86 773.1 0 86 131 99 773.1 0 99 132 336 773.1 0 336 133 45 773.1 0 45 134 51 773.1 0 51 135 83 773.1 0 83 136 80 773.1 0 80 137 131 773.1 0 131 138 70 773.1 0 70 139 55 773.1 0 55 140 181 773.1 0 181 141 335 773.1 0 335 142 306 773.1 0 306 143 275 773.1 0 275 144 185 773.1 0 185 145 294 773.1 0 294 146 445 773.1 0 445 147 12640 773.1 0 12640 148 5164 773.1 0 5164 149 11399 773.1 0 11399 150 259 773.1 0 259 151 183 773.1 0 183 152 389 773.1 0 389 153 6144 773.1 0 6144 154 4089 773.1 0 4089 155 5765 773.1 0 5765 156 270 773.1 0 270 157 246 773.1 0 246 158 94 773.1 0 94 159 36 773.1 0 36 160 56 773.1 0 56 161 56 773.1 0 56 162 123 773.1 0 123 163 63 773.1 0 63 164 48 773.1 0 48 165 46 773.1 0 46 166 41 773.1 0 41 167 53 773.1 0 53 168 185 773.1 0 185 169 206 773.1 0 206 170 136 773.1 0 136 171 74 773.1 0 74 172 46 773.1 0 46 173 30 773.1 0 30 174 32 773.1 0 32 175 64 773.1 0 64 176 47 773.1 0 47 177 45 773.1 0 45 178 31 773.1 0 31 179 36 773.1 0 36 180 38 773.1 0 38 181 92 773.1 0 92 182 106 773.1 0 106 183 277 773.1 0 277 184 168 773.1 0 168 185 269 773.1 0 269 186 203 773.1 0 203 187 63 773.1 0 63 188 57 773.1 0 57 189 125 773.1 0 125 190 125 773.1 0 125 191 417 773.1 0 417 192 39 773.1 0 39 193 91 773.1 0 91 194 32 773.1 0 32 195 48 773.1 0 48 196 26 773.1 0 26 197 53 773.1 0 53 198 108 773.1 0 108 199 56 773.1 0 56 200 36 773.1 0 36 201 45 773.1 0 45 202 187 773.1 0 187 203 116 773.1 0 116 204 155 773.1 0 155 205 44 773.1 0 44 206 29 773.1 0 29 207 41 773.1 0 41 208 39 773.1 0 39 209 32 773.1 0 32 210 27 773.1 0 27 211 37 773.1 0 37 212 26 773.1 0 26 213 33 773.1 0 33 214 31 773.1 0 31 215 33 773.1 0 33 216 175 773.1 0 175 217 122 773.1 0 122 218 158 773.1 0 158 219 28 773.1 0 28 220 35 773.1 0 35 221 46 773.1 0 46 222 37 773.1 0 37 223 25 773.1 0 25 224 26 773.1 0 26 225 26 773.1 0 26 226 72 773.1 0 72 227 41 773.1 0 41 228 58 773.1 0 58 229 31 773.1 0 31 230 36 773.1 0 36 231 35 773.1 0 35 232 41 773.1 0 41 233 59 773.1 0 59 234 92 773.1 0 92 235 125 773.1 0 125 236 101 773.1 0 101 237 55 773.1 0 55 238 55 773.1 0 55 239 42 773.1 0 42 240 52 773.1 0 52 241 26 773.1 0 26 242 47 773.1 0 47 243 30 773.1 0 30 244 25 773.1 0 25 245 41 773.1 0 41 246 75 773.1 0 75 247 34 773.1 0 34 248 41 773.1 0 41 249 65 773.1 0 65 250 54 773.1 0 54 251 49 773.1 0 49 252 36 773.1 0 36 253 46 773.1 0 46 254 42 773.1 0 42 255 36 773.1 0 36 256 27 773.1 0 27 257 38 773.1 0 38 258 38 773.1 0 38 259 47 773.1 0 47 260 35 773.1 0 35 261 37 773.1 0 37 262 80 773.1 0 80 263 45 773.1 0 45 264 29 773.1 0 29 265 53 773.1 0 53 266 53 773.1 0 53 267 94 773.1 0 94 268 94 773.1 0 94 269 64 773.1 0 64 270 103 773.1 0 103 271 104 773.1 0 104 272 122 773.1 0 122 273 81 773.1 0 81 274 81 773.1 0 81 275 112 773.1 0 112 276 82 773.1 0 82 277 90 773.1 0 90 278 166 773.1 0 166 279 403 773.1 0 403 280 746 773.1 0 746 281 2583 773.1 0 2583 282 1766 773.1 0 1766 283 2610 773.1 0 2610 284 112 773.1 0 112 285 110 773.1 0 110 286 100 773.1 0 100 287 46 773.1 0 46 288 34 773.1 0 34 289 87 773.1 0 87 290 276 773.1 0 276 291 317 773.1 0 317 292 294 773.1 0 294 293 176 773.1 0 176 294 123 773.1 0 123 295 104 773.1 0 104 296 96 773.1 0 96 297 100 773.1 0 100 298 55 773.1 0 55 299 42 773.1 0 42 300 41 773.1 0 41 301 41 773.1 0 41 Finished in 15.15 s (55 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 273,538 Reads with adapters: 4,808 (1.8%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 4,781 (1.7%) Total basepairs processed: 50,184,484 bp Total written (filtered): 1,273,623 bp (2.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4808 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 1 0.0 3 0 0 1 19 50 0.0 3 0 48 2 20 4444 0.0 4 4372 68 3 0 1 21 19 0.0 4 8 10 1 22 1 0.0 4 0 0 1 23 3 0.0 4 0 0 0 2 1 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 1 55 2 0.0 4 0 0 0 0 2 70 2 0.0 4 0 0 0 0 2 80 3 0.0 4 0 0 0 0 3 81 2 0.0 4 0 0 0 0 2 87 1 0.0 4 1 88 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 248 0.0 4 0 0 0 0 248 128 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 2 0.0 4 2 143 2 0.0 4 0 0 0 0 2 160 1 0.0 4 1 161 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 198 6 0.0 4 0 0 0 0 6 219 2 0.0 4 2 228 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 4,781 Reads with adapters: 427 (8.9%) Reads written (passing filters): 4,781 (100.0%) Total basepairs processed: 1,273,623 bp Total written (filtered): 1,269,209 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 427 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 2.6% G: 94.4% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 74.7 0 1 4 1 18.7 0 1 9 144 0.0 0 3 141 10 242 0.0 1 8 234 11 36 0.0 1 0 36 63 2 0.0 1 2 169 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.