This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.59 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 110,824 (3.5%) Reads that were too short: 76,518 (2.4%) Reads written (passing filters): 34,306 (1.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 8,792,367 bp (0.9%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 110824 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 483 773.1 0 483 7 9476 773.1 0 9476 8 348 773.1 0 348 9 12022 773.1 0 12022 10 42 773.1 0 42 11 40 773.1 0 40 12 42 773.1 0 42 13 31 773.1 0 31 14 35 773.1 0 35 15 44 773.1 0 44 16 36 773.1 0 36 17 56 773.1 0 56 18 54 773.1 0 54 19 78 773.1 0 78 20 34 773.1 0 34 21 38 773.1 0 38 22 31 773.1 0 31 23 39 773.1 0 39 24 38 773.1 0 38 25 32 773.1 0 32 26 30 773.1 0 30 27 32 773.1 0 32 28 38 773.1 0 38 29 59 773.1 0 59 30 128 773.1 0 128 31 61 773.1 0 61 32 47 773.1 0 47 33 53 773.1 0 53 34 31 773.1 0 31 35 61 773.1 0 61 36 36 773.1 0 36 37 42 773.1 0 42 38 48 773.1 0 48 39 28 773.1 0 28 40 57 773.1 0 57 41 45 773.1 0 45 42 53 773.1 0 53 43 34 773.1 0 34 44 33 773.1 0 33 45 28 773.1 0 28 46 35 773.1 0 35 47 54 773.1 0 54 48 52 773.1 0 52 49 69 773.1 0 69 50 48 773.1 0 48 51 56 773.1 0 56 52 49 773.1 0 49 53 62 773.1 0 62 54 87 773.1 0 87 55 36 773.1 0 36 56 36 773.1 0 36 57 35 773.1 0 35 58 64 773.1 0 64 59 56 773.1 0 56 60 54 773.1 0 54 61 56 773.1 0 56 62 44 773.1 0 44 63 58 773.1 0 58 64 54 773.1 0 54 65 31 773.1 0 31 66 41 773.1 0 41 67 31 773.1 0 31 68 43 773.1 0 43 69 45 773.1 0 45 70 53 773.1 0 53 71 103 773.1 0 103 72 135 773.1 0 135 73 114 773.1 0 114 74 80 773.1 0 80 75 32 773.1 0 32 76 29 773.1 0 29 77 37 773.1 0 37 78 30 773.1 0 30 79 43 773.1 0 43 80 50 773.1 0 50 81 58 773.1 0 58 82 53 773.1 0 53 83 33 773.1 0 33 84 54 773.1 0 54 85 45 773.1 0 45 86 33 773.1 0 33 87 41 773.1 0 41 88 32 773.1 0 32 89 38 773.1 0 38 90 28 773.1 0 28 91 49 773.1 0 49 92 67 773.1 0 67 93 63 773.1 0 63 94 66 773.1 0 66 95 62 773.1 0 62 96 61 773.1 0 61 97 89 773.1 0 89 98 35 773.1 0 35 99 40 773.1 0 40 100 27 773.1 0 27 101 43 773.1 0 43 102 34 773.1 0 34 103 67 773.1 0 67 104 38 773.1 0 38 105 38 773.1 0 38 106 33 773.1 0 33 107 39 773.1 0 39 108 42 773.1 0 42 109 35 773.1 0 35 110 32 773.1 0 32 111 40 773.1 0 40 112 39 773.1 0 39 113 35 773.1 0 35 114 31 773.1 0 31 115 48 773.1 0 48 116 33 773.1 0 33 117 67 773.1 0 67 118 35 773.1 0 35 119 132 773.1 0 132 120 68 773.1 0 68 121 37 773.1 0 37 122 32 773.1 0 32 123 87 773.1 0 87 124 356 773.1 0 356 125 53 773.1 0 53 126 60 773.1 0 60 127 78 773.1 0 78 128 32 773.1 0 32 129 43 773.1 0 43 130 137 773.1 0 137 131 404 773.1 0 404 132 244 773.1 0 244 133 214 773.1 0 214 134 183 773.1 0 183 135 64 773.1 0 64 136 180 773.1 0 180 137 444 773.1 0 444 138 376 773.1 0 376 139 238 773.1 0 238 140 72 773.1 0 72 141 54 773.1 0 54 142 54 773.1 0 54 143 50 773.1 0 50 144 31 773.1 0 31 145 40 773.1 0 40 146 65 773.1 0 65 147 27 773.1 0 27 148 41 773.1 0 41 149 35 773.1 0 35 150 52 773.1 0 52 151 147 773.1 0 147 152 95 773.1 0 95 153 90 773.1 0 90 154 37 773.1 0 37 155 44 773.1 0 44 156 34 773.1 0 34 157 32 773.1 0 32 158 48 773.1 0 48 159 31 773.1 0 31 160 27 773.1 0 27 161 33 773.1 0 33 162 33 773.1 0 33 163 38 773.1 0 38 164 31 773.1 0 31 165 49 773.1 0 49 166 65 773.1 0 65 167 37 773.1 0 37 168 37 773.1 0 37 169 32 773.1 0 32 170 28 773.1 0 28 171 22 773.1 0 22 172 26 773.1 0 26 173 30 773.1 0 30 174 38 773.1 0 38 175 28 773.1 0 28 176 39 773.1 0 39 177 73 773.1 0 73 178 232 773.1 0 232 179 60 773.1 0 60 180 30 773.1 0 30 181 40 773.1 0 40 182 20 773.1 0 20 183 39 773.1 0 39 184 52 773.1 0 52 185 48 773.1 0 48 186 136 773.1 0 136 187 129 773.1 0 129 188 119 773.1 0 119 189 99 773.1 0 99 190 66 773.1 0 66 191 37 773.1 0 37 192 43 773.1 0 43 193 43 773.1 0 43 194 36 773.1 0 36 195 38 773.1 0 38 196 46 773.1 0 46 197 75 773.1 0 75 198 40 773.1 0 40 199 48 773.1 0 48 200 41 773.1 0 41 201 34 773.1 0 34 202 47 773.1 0 47 203 47 773.1 0 47 204 47 773.1 0 47 205 63 773.1 0 63 206 56 773.1 0 56 207 43 773.1 0 43 208 43 773.1 0 43 209 32 773.1 0 32 210 61 773.1 0 61 211 73 773.1 0 73 212 458 773.1 0 458 213 502 773.1 0 502 214 346 773.1 0 346 215 78 773.1 0 78 216 120 773.1 0 120 217 125 773.1 0 125 218 338 773.1 0 338 219 113 773.1 0 113 220 141 773.1 0 141 221 49 773.1 0 49 222 48 773.1 0 48 223 50 773.1 0 50 224 45 773.1 0 45 225 64 773.1 0 64 226 63 773.1 0 63 227 47 773.1 0 47 228 65 773.1 0 65 229 54 773.1 0 54 230 54 773.1 0 54 231 58 773.1 0 58 232 95 773.1 0 95 233 89 773.1 0 89 234 108 773.1 0 108 235 197 773.1 0 197 236 694 773.1 0 694 237 416 773.1 0 416 238 534 773.1 0 534 239 158 773.1 0 158 240 58 773.1 0 58 241 229 773.1 0 229 242 217 773.1 0 217 243 1142 773.1 0 1142 244 1141 773.1 0 1141 245 1206 773.1 0 1206 246 426 773.1 0 426 247 161 773.1 0 161 248 121 773.1 0 121 249 112 773.1 0 112 250 183 773.1 0 183 251 159 773.1 0 159 252 169 773.1 0 169 253 99 773.1 0 99 254 141 773.1 0 141 255 368 773.1 0 368 256 612 773.1 0 612 257 742 773.1 0 742 258 527 773.1 0 527 259 256 773.1 0 256 260 1356 773.1 0 1356 261 746 773.1 0 746 262 1043 773.1 0 1043 263 327 773.1 0 327 264 79 773.1 0 79 265 206 773.1 0 206 266 207 773.1 0 207 267 225 773.1 0 225 268 119 773.1 0 119 269 102 773.1 0 102 270 124 773.1 0 124 271 178 773.1 0 178 272 204 773.1 0 204 273 287 773.1 0 287 274 163 773.1 0 163 275 234 773.1 0 234 276 407 773.1 0 407 277 226 773.1 0 226 278 159 773.1 0 159 279 379 773.1 0 379 280 485 773.1 0 485 281 798 773.1 0 798 282 558 773.1 0 558 283 455 773.1 0 455 284 564 773.1 0 564 285 847 773.1 0 847 286 15983 773.1 0 15983 287 13704 773.1 0 13704 288 19415 773.1 0 19415 289 160 773.1 0 160 290 91 773.1 0 91 291 118 773.1 0 118 292 168 773.1 0 168 293 126 773.1 0 126 294 122 773.1 0 122 295 162 773.1 0 162 296 539 773.1 0 539 297 473 773.1 0 473 298 600 773.1 0 600 299 467 773.1 0 467 300 160 773.1 0 160 301 62 773.1 0 62 Finished in 2.30 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 34,306 Reads with adapters: 12,564 (36.6%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 12,523 (36.5%) Total basepairs processed: 8,792,367 bp Total written (filtered): 3,436,444 bp (39.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12564 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 6 0.0 3 0 0 4 2 16 103 0.0 3 0 86 9 8 17 12222 0.0 3 11368 677 132 45 18 64 0.0 3 22 34 5 3 19 38 0.0 3 10 2 15 11 20 32 0.0 3 2 0 2 28 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 32 2 0.0 3 0 0 0 2 33 1 0.0 3 1 41 7 0.0 3 0 0 0 7 42 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 5 0.0 3 0 0 0 5 62 2 0.0 3 0 0 1 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 1 89 1 0.0 3 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 1 150 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 12,523 Reads with adapters: 410 (3.3%) Reads written (passing filters): 12,523 (100.0%) Total basepairs processed: 3,436,444 bp Total written (filtered): 3,435,191 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 410 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.5% C: 10.5% G: 25.6% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 388 195.7 0 388 4 21 48.9 0 21 5 1 12.2 0 1