This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.03 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 79,060 (3.1%) Reads that were too short: 28,730 (1.1%) Reads written (passing filters): 50,330 (2.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,266,789 bp (1.5%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 79060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 628.5 0 208 7 640 628.5 0 640 8 9610 628.5 0 9610 9 8796 628.5 0 8796 10 97 628.5 0 97 11 76 628.5 0 76 12 88 628.5 0 88 13 81 628.5 0 81 14 83 628.5 0 83 15 75 628.5 0 75 16 79 628.5 0 79 17 88 628.5 0 88 18 53 628.5 0 53 19 54 628.5 0 54 20 106 628.5 0 106 21 90 628.5 0 90 22 105 628.5 0 105 23 80 628.5 0 80 24 93 628.5 0 93 25 87 628.5 0 87 26 63 628.5 0 63 27 92 628.5 0 92 28 82 628.5 0 82 29 79 628.5 0 79 30 87 628.5 0 87 31 93 628.5 0 93 32 97 628.5 0 97 33 90 628.5 0 90 34 79 628.5 0 79 35 87 628.5 0 87 36 97 628.5 0 97 37 90 628.5 0 90 38 171 628.5 0 171 39 104 628.5 0 104 40 193 628.5 0 193 41 91 628.5 0 91 42 86 628.5 0 86 43 69 628.5 0 69 44 65 628.5 0 65 45 68 628.5 0 68 46 86 628.5 0 86 47 76 628.5 0 76 48 71 628.5 0 71 49 82 628.5 0 82 50 75 628.5 0 75 51 104 628.5 0 104 52 113 628.5 0 113 53 88 628.5 0 88 54 90 628.5 0 90 55 111 628.5 0 111 56 96 628.5 0 96 57 78 628.5 0 78 58 94 628.5 0 94 59 84 628.5 0 84 60 127 628.5 0 127 61 102 628.5 0 102 62 86 628.5 0 86 63 117 628.5 0 117 64 108 628.5 0 108 65 93 628.5 0 93 66 112 628.5 0 112 67 121 628.5 0 121 68 147 628.5 0 147 69 122 628.5 0 122 70 99 628.5 0 99 71 99 628.5 0 99 72 106 628.5 0 106 73 98 628.5 0 98 74 175 628.5 0 175 75 145 628.5 0 145 76 104 628.5 0 104 77 88 628.5 0 88 78 140 628.5 0 140 79 113 628.5 0 113 80 108 628.5 0 108 81 91 628.5 0 91 82 134 628.5 0 134 83 87 628.5 0 87 84 102 628.5 0 102 85 70 628.5 0 70 86 95 628.5 0 95 87 123 628.5 0 123 88 114 628.5 0 114 89 130 628.5 0 130 90 86 628.5 0 86 91 150 628.5 0 150 92 112 628.5 0 112 93 110 628.5 0 110 94 168 628.5 0 168 95 203 628.5 0 203 96 207 628.5 0 207 97 1513 628.5 0 1513 98 805 628.5 0 805 99 1233 628.5 0 1233 100 321 628.5 0 321 101 104 628.5 0 104 102 64 628.5 0 64 103 82 628.5 0 82 104 95 628.5 0 95 105 105 628.5 0 105 106 108 628.5 0 108 107 143 628.5 0 143 108 190 628.5 0 190 109 231 628.5 0 231 110 151 628.5 0 151 111 636 628.5 0 636 112 666 628.5 0 666 113 736 628.5 0 736 114 100 628.5 0 100 115 81 628.5 0 81 116 80 628.5 0 80 117 110 628.5 0 110 118 96 628.5 0 96 119 99 628.5 0 99 120 95 628.5 0 95 121 80 628.5 0 80 122 100 628.5 0 100 123 72 628.5 0 72 124 88 628.5 0 88 125 99 628.5 0 99 126 114 628.5 0 114 127 83 628.5 0 83 128 121 628.5 0 121 129 133 628.5 0 133 130 164 628.5 0 164 131 131 628.5 0 131 132 173 628.5 0 173 133 158 628.5 0 158 134 314 628.5 0 314 135 119 628.5 0 119 136 135 628.5 0 135 137 100 628.5 0 100 138 90 628.5 0 90 139 88 628.5 0 88 140 112 628.5 0 112 141 119 628.5 0 119 142 124 628.5 0 124 143 104 628.5 0 104 144 106 628.5 0 106 145 106 628.5 0 106 146 143 628.5 0 143 147 180 628.5 0 180 148 168 628.5 0 168 149 124 628.5 0 124 150 86 628.5 0 86 151 112 628.5 0 112 152 117 628.5 0 117 153 123 628.5 0 123 154 243 628.5 0 243 155 390 628.5 0 390 156 799 628.5 0 799 157 842 628.5 0 842 158 91 628.5 0 91 159 98 628.5 0 98 160 112 628.5 0 112 161 106 628.5 0 106 162 98 628.5 0 98 163 75 628.5 0 75 164 117 628.5 0 117 165 102 628.5 0 102 166 315 628.5 0 315 167 232 628.5 0 232 168 327 628.5 0 327 169 90 628.5 0 90 170 103 628.5 0 103 171 94 628.5 0 94 172 101 628.5 0 101 173 67 628.5 0 67 174 92 628.5 0 92 175 96 628.5 0 96 176 91 628.5 0 91 177 87 628.5 0 87 178 105 628.5 0 105 179 102 628.5 0 102 180 127 628.5 0 127 181 129 628.5 0 129 182 101 628.5 0 101 183 105 628.5 0 105 184 68 628.5 0 68 185 115 628.5 0 115 186 126 628.5 0 126 187 107 628.5 0 107 188 121 628.5 0 121 189 115 628.5 0 115 190 139 628.5 0 139 191 97 628.5 0 97 192 151 628.5 0 151 193 465 628.5 0 465 194 864 628.5 0 864 195 914 628.5 0 914 196 443 628.5 0 443 197 148 628.5 0 148 198 150 628.5 0 150 199 155 628.5 0 155 200 340 628.5 0 340 201 333 628.5 0 333 202 383 628.5 0 383 203 118 628.5 0 118 204 101 628.5 0 101 205 100 628.5 0 100 206 118 628.5 0 118 207 153 628.5 0 153 208 120 628.5 0 120 209 101 628.5 0 101 210 116 628.5 0 116 211 108 628.5 0 108 212 98 628.5 0 98 213 106 628.5 0 106 214 117 628.5 0 117 215 155 628.5 0 155 216 187 628.5 0 187 217 186 628.5 0 186 218 142 628.5 0 142 219 121 628.5 0 121 220 626 628.5 0 626 221 740 628.5 0 740 222 805 628.5 0 805 223 897 628.5 0 897 224 99 628.5 0 99 225 90 628.5 0 90 226 97 628.5 0 97 227 114 628.5 0 114 228 122 628.5 0 122 229 129 628.5 0 129 230 100 628.5 0 100 231 80 628.5 0 80 232 93 628.5 0 93 233 129 628.5 0 129 234 84 628.5 0 84 235 97 628.5 0 97 236 127 628.5 0 127 237 142 628.5 0 142 238 198 628.5 0 198 239 458 628.5 0 458 240 187 628.5 0 187 241 281 628.5 0 281 242 247 628.5 0 247 243 720 628.5 0 720 244 377 628.5 0 377 245 538 628.5 0 538 246 278 628.5 0 278 247 94 628.5 0 94 248 134 628.5 0 134 249 141 628.5 0 141 250 165 628.5 0 165 251 211 628.5 0 211 252 233 628.5 0 233 253 240 628.5 0 240 254 510 628.5 0 510 255 555 628.5 0 555 256 978 628.5 0 978 257 155 628.5 0 155 258 174 628.5 0 174 259 165 628.5 0 165 260 179 628.5 0 179 261 212 628.5 0 212 262 1615 628.5 0 1615 263 1893 628.5 0 1893 264 3633 628.5 0 3633 265 252 628.5 0 252 266 111 628.5 0 111 267 84 628.5 0 84 268 110 628.5 0 110 269 157 628.5 0 157 270 167 628.5 0 167 271 183 628.5 0 183 272 169 628.5 0 169 273 205 628.5 0 205 274 127 628.5 0 127 275 157 628.5 0 157 276 175 628.5 0 175 277 128 628.5 0 128 278 102 628.5 0 102 279 147 628.5 0 147 280 337 628.5 0 337 281 152 628.5 0 152 282 275 628.5 0 275 283 171 628.5 0 171 284 128 628.5 0 128 285 130 628.5 0 130 286 135 628.5 0 135 287 112 628.5 0 112 288 130 628.5 0 130 289 214 628.5 0 214 290 194 628.5 0 194 291 220 628.5 0 220 292 145 628.5 0 145 293 603 628.5 0 603 294 643 628.5 0 643 295 222 628.5 0 222 296 143 628.5 0 143 297 136 628.5 0 136 298 147 628.5 0 147 299 167 628.5 0 167 300 212 628.5 0 212 301 168 628.5 0 168 Finished in 2.16 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 50,330 Reads with adapters: 9,888 (19.6%) Reads that were too short: 100 (0.2%) Reads written (passing filters): 9,788 (19.4%) Total basepairs processed: 11,266,789 bp Total written (filtered): 2,679,677 bp (23.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9888 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 8 0.0 3 0 0 6 2 16 160 0.0 3 0 136 19 5 17 9378 0.0 3 8466 708 169 35 18 23 0.0 3 3 18 1 1 19 40 0.0 3 1 0 23 16 20 37 0.0 3 1 0 2 34 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 16 0.0 3 0 0 1 15 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 55 8 0.0 3 0 0 0 8 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 60 4 0.0 3 0 0 0 4 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 76 31 0.0 3 0 0 0 31 77 6 0.0 3 0 0 0 6 78 1 0.0 3 0 0 0 1 79 5 0.0 3 0 0 0 5 80 4 0.0 3 0 0 0 4 81 3 0.0 3 0 0 0 3 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 15 0.0 3 0 0 0 15 94 7 0.0 3 0 0 0 7 96 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 104 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 138 4 0.0 3 0 0 0 4 139 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 35 0.0 3 0 0 1 34 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 194 5 0.0 3 0 0 0 5 195 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 253 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 9,788 Reads with adapters: 29 (0.3%) Reads written (passing filters): 9,788 (100.0%) Total basepairs processed: 2,679,677 bp Total written (filtered): 2,679,566 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 29 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.7% C: 13.8% G: 62.1% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 152.9 0 8 4 18 38.2 0 18 5 3 9.6 0 3