This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.38 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 58,961 (2.3%) Reads that were too short: 21,577 (0.8%) Reads written (passing filters): 37,384 (1.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 9,045,777 bp (1.2%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 58961 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 153 628.5 0 153 7 458 628.5 0 458 8 9467 628.5 0 9467 9 10199 628.5 0 10199 10 65 628.5 0 65 11 43 628.5 0 43 12 47 628.5 0 47 13 47 628.5 0 47 14 52 628.5 0 52 15 40 628.5 0 40 16 54 628.5 0 54 17 42 628.5 0 42 18 37 628.5 0 37 19 37 628.5 0 37 20 48 628.5 0 48 21 54 628.5 0 54 22 52 628.5 0 52 23 42 628.5 0 42 24 43 628.5 0 43 25 41 628.5 0 41 26 48 628.5 0 48 27 51 628.5 0 51 28 53 628.5 0 53 29 36 628.5 0 36 30 47 628.5 0 47 31 56 628.5 0 56 32 58 628.5 0 58 33 61 628.5 0 61 34 42 628.5 0 42 35 40 628.5 0 40 36 63 628.5 0 63 37 52 628.5 0 52 38 105 628.5 0 105 39 55 628.5 0 55 40 77 628.5 0 77 41 66 628.5 0 66 42 52 628.5 0 52 43 47 628.5 0 47 44 46 628.5 0 46 45 50 628.5 0 50 46 40 628.5 0 40 47 66 628.5 0 66 48 57 628.5 0 57 49 28 628.5 0 28 50 48 628.5 0 48 51 68 628.5 0 68 52 59 628.5 0 59 53 58 628.5 0 58 54 48 628.5 0 48 55 62 628.5 0 62 56 63 628.5 0 63 57 46 628.5 0 46 58 46 628.5 0 46 59 58 628.5 0 58 60 94 628.5 0 94 61 61 628.5 0 61 62 68 628.5 0 68 63 73 628.5 0 73 64 62 628.5 0 62 65 50 628.5 0 50 66 54 628.5 0 54 67 72 628.5 0 72 68 71 628.5 0 71 69 61 628.5 0 61 70 64 628.5 0 64 71 67 628.5 0 67 72 59 628.5 0 59 73 44 628.5 0 44 74 128 628.5 0 128 75 66 628.5 0 66 76 54 628.5 0 54 77 56 628.5 0 56 78 82 628.5 0 82 79 65 628.5 0 65 80 62 628.5 0 62 81 51 628.5 0 51 82 79 628.5 0 79 83 56 628.5 0 56 84 88 628.5 0 88 85 54 628.5 0 54 86 59 628.5 0 59 87 77 628.5 0 77 88 82 628.5 0 82 89 67 628.5 0 67 90 46 628.5 0 46 91 96 628.5 0 96 92 63 628.5 0 63 93 71 628.5 0 71 94 124 628.5 0 124 95 103 628.5 0 103 96 93 628.5 0 93 97 439 628.5 0 439 98 207 628.5 0 207 99 296 628.5 0 296 100 139 628.5 0 139 101 61 628.5 0 61 102 54 628.5 0 54 103 53 628.5 0 53 104 63 628.5 0 63 105 64 628.5 0 64 106 71 628.5 0 71 107 69 628.5 0 69 108 91 628.5 0 91 109 107 628.5 0 107 110 89 628.5 0 89 111 689 628.5 0 689 112 757 628.5 0 757 113 641 628.5 0 641 114 52 628.5 0 52 115 43 628.5 0 43 116 59 628.5 0 59 117 57 628.5 0 57 118 57 628.5 0 57 119 60 628.5 0 60 120 52 628.5 0 52 121 41 628.5 0 41 122 61 628.5 0 61 123 55 628.5 0 55 124 53 628.5 0 53 125 67 628.5 0 67 126 53 628.5 0 53 127 69 628.5 0 69 128 70 628.5 0 70 129 78 628.5 0 78 130 60 628.5 0 60 131 66 628.5 0 66 132 91 628.5 0 91 133 70 628.5 0 70 134 135 628.5 0 135 135 60 628.5 0 60 136 65 628.5 0 65 137 49 628.5 0 49 138 70 628.5 0 70 139 59 628.5 0 59 140 54 628.5 0 54 141 70 628.5 0 70 142 54 628.5 0 54 143 46 628.5 0 46 144 66 628.5 0 66 145 80 628.5 0 80 146 90 628.5 0 90 147 99 628.5 0 99 148 80 628.5 0 80 149 66 628.5 0 66 150 49 628.5 0 49 151 51 628.5 0 51 152 70 628.5 0 70 153 53 628.5 0 53 154 119 628.5 0 119 155 149 628.5 0 149 156 329 628.5 0 329 157 384 628.5 0 384 158 61 628.5 0 61 159 37 628.5 0 37 160 58 628.5 0 58 161 65 628.5 0 65 162 60 628.5 0 60 163 52 628.5 0 52 164 81 628.5 0 81 165 65 628.5 0 65 166 138 628.5 0 138 167 103 628.5 0 103 168 97 628.5 0 97 169 44 628.5 0 44 170 49 628.5 0 49 171 46 628.5 0 46 172 57 628.5 0 57 173 48 628.5 0 48 174 67 628.5 0 67 175 45 628.5 0 45 176 56 628.5 0 56 177 58 628.5 0 58 178 58 628.5 0 58 179 71 628.5 0 71 180 82 628.5 0 82 181 85 628.5 0 85 182 64 628.5 0 64 183 62 628.5 0 62 184 56 628.5 0 56 185 57 628.5 0 57 186 50 628.5 0 50 187 69 628.5 0 69 188 62 628.5 0 62 189 67 628.5 0 67 190 72 628.5 0 72 191 76 628.5 0 76 192 79 628.5 0 79 193 146 628.5 0 146 194 615 628.5 0 615 195 602 628.5 0 602 196 346 628.5 0 346 197 67 628.5 0 67 198 68 628.5 0 68 199 71 628.5 0 71 200 119 628.5 0 119 201 107 628.5 0 107 202 107 628.5 0 107 203 49 628.5 0 49 204 53 628.5 0 53 205 63 628.5 0 63 206 69 628.5 0 69 207 61 628.5 0 61 208 64 628.5 0 64 209 74 628.5 0 74 210 63 628.5 0 63 211 62 628.5 0 62 212 49 628.5 0 49 213 63 628.5 0 63 214 65 628.5 0 65 215 74 628.5 0 74 216 73 628.5 0 73 217 80 628.5 0 80 218 61 628.5 0 61 219 56 628.5 0 56 220 210 628.5 0 210 221 212 628.5 0 212 222 283 628.5 0 283 223 326 628.5 0 326 224 64 628.5 0 64 225 59 628.5 0 59 226 63 628.5 0 63 227 64 628.5 0 64 228 71 628.5 0 71 229 71 628.5 0 71 230 56 628.5 0 56 231 46 628.5 0 46 232 49 628.5 0 49 233 67 628.5 0 67 234 63 628.5 0 63 235 66 628.5 0 66 236 64 628.5 0 64 237 90 628.5 0 90 238 169 628.5 0 169 239 575 628.5 0 575 240 103 628.5 0 103 241 191 628.5 0 191 242 113 628.5 0 113 243 391 628.5 0 391 244 158 628.5 0 158 245 201 628.5 0 201 246 96 628.5 0 96 247 70 628.5 0 70 248 91 628.5 0 91 249 118 628.5 0 118 250 106 628.5 0 106 251 119 628.5 0 119 252 147 628.5 0 147 253 172 628.5 0 172 254 203 628.5 0 203 255 210 628.5 0 210 256 415 628.5 0 415 257 92 628.5 0 92 258 92 628.5 0 92 259 105 628.5 0 105 260 139 628.5 0 139 261 155 628.5 0 155 262 1767 628.5 0 1767 263 2162 628.5 0 2162 264 4798 628.5 0 4798 265 137 628.5 0 137 266 64 628.5 0 64 267 64 628.5 0 64 268 62 628.5 0 62 269 75 628.5 0 75 270 74 628.5 0 74 271 85 628.5 0 85 272 81 628.5 0 81 273 95 628.5 0 95 274 75 628.5 0 75 275 91 628.5 0 91 276 76 628.5 0 76 277 72 628.5 0 72 278 60 628.5 0 60 279 104 628.5 0 104 280 163 628.5 0 163 281 111 628.5 0 111 282 113 628.5 0 113 283 97 628.5 0 97 284 72 628.5 0 72 285 79 628.5 0 79 286 96 628.5 0 96 287 91 628.5 0 91 288 82 628.5 0 82 289 131 628.5 0 131 290 134 628.5 0 134 291 161 628.5 0 161 292 133 628.5 0 133 293 940 628.5 0 940 294 1038 628.5 0 1038 295 276 628.5 0 276 296 76 628.5 0 76 297 90 628.5 0 90 298 92 628.5 0 92 299 109 628.5 0 109 300 122 628.5 0 122 301 88 628.5 0 88 Finished in 1.88 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 37,384 Reads with adapters: 11,353 (30.4%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 11,349 (30.4%) Total basepairs processed: 9,045,777 bp Total written (filtered): 3,017,470 bp (33.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11353 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 160 0.0 3 0 158 2 20 10491 0.0 4 10383 108 21 26 0.0 4 7 19 22 2 0.0 4 0 1 1 23 8 0.0 4 0 0 0 7 1 31 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 60 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 126 9 0.0 4 0 0 0 0 9 127 644 0.0 4 0 0 0 0 644 128 2 0.0 4 0 0 0 0 2 164 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 11,349 Reads with adapters: 1,827 (16.1%) Reads written (passing filters): 11,349 (100.0%) Total basepairs processed: 3,017,470 bp Total written (filtered): 2,999,806 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1827 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.3% G: 94.1% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 177.3 0 3 4 3 44.3 0 3 5 1 11.1 0 1 9 1024 0.0 0 34 990 10 652 0.0 1 23 629 11 140 0.0 1 1 139 12 2 0.0 1 0 2 169 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.