This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.21 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 207,620 (8.1%) Reads that were too short: 21,809 (0.8%) Reads written (passing filters): 185,811 (7.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 40,234,408 bp (5.2%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 207620 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 854 628.5 0 854 7 906 628.5 0 906 8 785 628.5 0 785 9 125 628.5 0 125 10 285 628.5 0 285 11 191 628.5 0 191 12 184 628.5 0 184 13 147 628.5 0 147 14 110 628.5 0 110 15 126 628.5 0 126 16 115 628.5 0 115 17 110 628.5 0 110 18 124 628.5 0 124 19 182 628.5 0 182 20 137 628.5 0 137 21 150 628.5 0 150 22 144 628.5 0 144 23 137 628.5 0 137 24 129 628.5 0 129 25 135 628.5 0 135 26 126 628.5 0 126 27 97 628.5 0 97 28 150 628.5 0 150 29 290 628.5 0 290 30 108 628.5 0 108 31 110 628.5 0 110 32 101 628.5 0 101 33 113 628.5 0 113 34 106 628.5 0 106 35 125 628.5 0 125 36 143 628.5 0 143 37 151 628.5 0 151 38 225 628.5 0 225 39 357 628.5 0 357 40 169 628.5 0 169 41 259 628.5 0 259 42 169 628.5 0 169 43 169 628.5 0 169 44 274 628.5 0 274 45 173 628.5 0 173 46 184 628.5 0 184 47 169 628.5 0 169 48 224 628.5 0 224 49 401 628.5 0 401 50 386 628.5 0 386 51 584 628.5 0 584 52 736 628.5 0 736 53 889 628.5 0 889 54 2171 628.5 0 2171 55 4093 628.5 0 4093 56 42343 628.5 0 42343 57 22631 628.5 0 22631 58 45988 628.5 0 45988 59 292 628.5 0 292 60 161 628.5 0 161 61 181 628.5 0 181 62 554 628.5 0 554 63 221 628.5 0 221 64 486 628.5 0 486 65 96 628.5 0 96 66 128 628.5 0 128 67 121 628.5 0 121 68 114 628.5 0 114 69 132 628.5 0 132 70 140 628.5 0 140 71 117 628.5 0 117 72 118 628.5 0 118 73 113 628.5 0 113 74 136 628.5 0 136 75 112 628.5 0 112 76 96 628.5 0 96 77 81 628.5 0 81 78 77 628.5 0 77 79 117 628.5 0 117 80 149 628.5 0 149 81 146 628.5 0 146 82 124 628.5 0 124 83 186 628.5 0 186 84 161 628.5 0 161 85 176 628.5 0 176 86 156 628.5 0 156 87 146 628.5 0 146 88 206 628.5 0 206 89 172 628.5 0 172 90 115 628.5 0 115 91 85 628.5 0 85 92 83 628.5 0 83 93 97 628.5 0 97 94 78 628.5 0 78 95 107 628.5 0 107 96 87 628.5 0 87 97 102 628.5 0 102 98 73 628.5 0 73 99 96 628.5 0 96 100 106 628.5 0 106 101 101 628.5 0 101 102 89 628.5 0 89 103 211 628.5 0 211 104 328 628.5 0 328 105 316 628.5 0 316 106 91 628.5 0 91 107 86 628.5 0 86 108 105 628.5 0 105 109 97 628.5 0 97 110 99 628.5 0 99 111 108 628.5 0 108 112 91 628.5 0 91 113 219 628.5 0 219 114 154 628.5 0 154 115 107 628.5 0 107 116 97 628.5 0 97 117 127 628.5 0 127 118 117 628.5 0 117 119 405 628.5 0 405 120 108 628.5 0 108 121 106 628.5 0 106 122 81 628.5 0 81 123 141 628.5 0 141 124 396 628.5 0 396 125 809 628.5 0 809 126 821 628.5 0 821 127 182 628.5 0 182 128 130 628.5 0 130 129 140 628.5 0 140 130 151 628.5 0 151 131 98 628.5 0 98 132 128 628.5 0 128 133 104 628.5 0 104 134 93 628.5 0 93 135 118 628.5 0 118 136 106 628.5 0 106 137 97 628.5 0 97 138 143 628.5 0 143 139 124 628.5 0 124 140 252 628.5 0 252 141 466 628.5 0 466 142 222 628.5 0 222 143 196 628.5 0 196 144 189 628.5 0 189 145 330 628.5 0 330 146 493 628.5 0 493 147 3636 628.5 0 3636 148 1681 628.5 0 1681 149 2976 628.5 0 2976 150 1149 628.5 0 1149 151 333 628.5 0 333 152 943 628.5 0 943 153 190 628.5 0 190 154 156 628.5 0 156 155 131 628.5 0 131 156 152 628.5 0 152 157 128 628.5 0 128 158 133 628.5 0 133 159 192 628.5 0 192 160 878 628.5 0 878 161 603 628.5 0 603 162 495 628.5 0 495 163 163 628.5 0 163 164 155 628.5 0 155 165 302 628.5 0 302 166 466 628.5 0 466 167 5229 628.5 0 5229 168 1207 628.5 0 1207 169 3807 628.5 0 3807 170 190 628.5 0 190 171 309 628.5 0 309 172 309 628.5 0 309 173 321 628.5 0 321 174 307 628.5 0 307 175 606 628.5 0 606 176 642 628.5 0 642 177 878 628.5 0 878 178 207 628.5 0 207 179 126 628.5 0 126 180 173 628.5 0 173 181 177 628.5 0 177 182 167 628.5 0 167 183 186 628.5 0 186 184 143 628.5 0 143 185 154 628.5 0 154 186 183 628.5 0 183 187 238 628.5 0 238 188 202 628.5 0 202 189 139 628.5 0 139 190 162 628.5 0 162 191 184 628.5 0 184 192 246 628.5 0 246 193 242 628.5 0 242 194 334 628.5 0 334 195 965 628.5 0 965 196 1197 628.5 0 1197 197 1003 628.5 0 1003 198 763 628.5 0 763 199 456 628.5 0 456 200 3020 628.5 0 3020 201 1771 628.5 0 1771 202 2284 628.5 0 2284 203 190 628.5 0 190 204 79 628.5 0 79 205 111 628.5 0 111 206 120 628.5 0 120 207 172 628.5 0 172 208 135 628.5 0 135 209 100 628.5 0 100 210 80 628.5 0 80 211 86 628.5 0 86 212 125 628.5 0 125 213 102 628.5 0 102 214 116 628.5 0 116 215 117 628.5 0 117 216 112 628.5 0 112 217 101 628.5 0 101 218 104 628.5 0 104 219 262 628.5 0 262 220 260 628.5 0 260 221 344 628.5 0 344 222 134 628.5 0 134 223 86 628.5 0 86 224 73 628.5 0 73 225 123 628.5 0 123 226 95 628.5 0 95 227 94 628.5 0 94 228 168 628.5 0 168 229 429 628.5 0 429 230 292 628.5 0 292 231 336 628.5 0 336 232 692 628.5 0 692 233 378 628.5 0 378 234 522 628.5 0 522 235 85 628.5 0 85 236 94 628.5 0 94 237 105 628.5 0 105 238 107 628.5 0 107 239 121 628.5 0 121 240 142 628.5 0 142 241 156 628.5 0 156 242 170 628.5 0 170 243 148 628.5 0 148 244 86 628.5 0 86 245 96 628.5 0 96 246 111 628.5 0 111 247 106 628.5 0 106 248 175 628.5 0 175 249 134 628.5 0 134 250 166 628.5 0 166 251 119 628.5 0 119 252 113 628.5 0 113 253 90 628.5 0 90 254 83 628.5 0 83 255 85 628.5 0 85 256 94 628.5 0 94 257 111 628.5 0 111 258 151 628.5 0 151 259 106 628.5 0 106 260 126 628.5 0 126 261 174 628.5 0 174 262 185 628.5 0 185 263 144 628.5 0 144 264 98 628.5 0 98 265 92 628.5 0 92 266 148 628.5 0 148 267 174 628.5 0 174 268 341 628.5 0 341 269 421 628.5 0 421 270 422 628.5 0 422 271 226 628.5 0 226 272 123 628.5 0 123 273 215 628.5 0 215 274 172 628.5 0 172 275 191 628.5 0 191 276 244 628.5 0 244 277 194 628.5 0 194 278 328 628.5 0 328 279 258 628.5 0 258 280 258 628.5 0 258 281 424 628.5 0 424 282 472 628.5 0 472 283 426 628.5 0 426 284 153 628.5 0 153 285 89 628.5 0 89 286 95 628.5 0 95 287 172 628.5 0 172 288 244 628.5 0 244 289 358 628.5 0 358 290 260 628.5 0 260 291 430 628.5 0 430 292 245 628.5 0 245 293 345 628.5 0 345 294 387 628.5 0 387 295 275 628.5 0 275 296 162 628.5 0 162 297 143 628.5 0 143 298 111 628.5 0 111 299 126 628.5 0 126 300 358 628.5 0 358 301 624 628.5 0 624 Finished in 6.94 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 185,811 Reads with adapters: 1,445 (0.8%) Reads that were too short: 327 (0.2%) Reads written (passing filters): 1,118 (0.6%) Total basepairs processed: 40,234,408 bp Total written (filtered): 262,292 bp (0.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1445 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 5 0.0 3 0 4 0 1 17 778 0.0 3 710 56 10 2 18 18 0.0 3 4 9 0 5 19 13 0.0 3 1 4 3 5 20 20 0.0 3 0 0 0 20 21 1 0.0 3 0 0 0 1 22 8 0.0 3 0 0 0 8 23 7 0.0 3 0 0 0 7 25 2 0.0 3 0 0 1 1 26 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 1 1 32 2 0.0 3 0 0 0 2 34 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 8 0.0 3 0 0 2 6 45 2 0.0 3 0 0 0 2 46 5 0.0 3 0 0 0 5 47 9 0.0 3 0 0 1 8 48 7 0.0 3 0 0 0 7 49 6 0.0 3 0 0 1 5 50 1 0.0 3 0 0 0 1 51 5 0.0 3 0 0 0 5 52 3 0.0 3 0 0 0 3 53 41 0.0 3 0 0 2 39 56 10 0.0 3 0 0 0 10 57 9 0.0 3 0 0 1 8 58 9 0.0 3 0 0 0 9 59 2 0.0 3 0 0 0 2 61 5 0.0 3 0 0 0 5 64 2 0.0 3 0 0 0 2 65 7 0.0 3 0 0 0 7 67 4 0.0 3 0 0 0 4 68 14 0.0 3 0 0 0 14 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 1 98 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 107 4 0.0 3 0 0 0 4 108 5 0.0 3 0 0 0 5 109 1 0.0 3 0 0 0 1 111 8 0.0 3 0 0 0 8 113 3 0.0 3 0 0 0 3 116 20 0.0 3 0 0 0 20 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 4 0.0 3 0 0 0 4 120 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 1 133 2 0.0 3 0 0 1 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 19 0.0 3 0 0 0 19 139 2 0.0 3 0 0 0 2 140 4 0.0 3 0 0 0 4 141 5 0.0 3 0 0 0 5 142 7 0.0 3 0 0 0 7 143 7 0.0 3 0 0 0 7 144 8 0.0 3 0 1 1 6 145 86 0.0 3 0 0 4 82 146 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 168 4 0.0 3 0 0 0 4 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 176 5 0.0 3 0 0 0 5 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 3 0.0 3 0 0 0 3 189 3 0.0 3 0 0 0 3 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 220 2 0.0 3 0 0 0 2 221 31 0.0 3 0 0 0 31 222 4 0.0 3 0 0 0 4 223 3 0.0 3 0 0 1 2 224 4 0.0 3 0 0 0 4 229 4 0.0 3 0 0 0 4 230 2 0.0 3 0 0 0 2 231 21 0.0 3 0 0 0 21 232 9 0.0 3 0 0 0 9 233 4 0.0 3 0 0 0 4 234 2 0.0 3 0 0 0 2 235 2 0.0 3 0 0 0 2 237 2 0.0 3 0 0 0 2 238 4 0.0 3 0 0 0 4 239 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 242 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 1 247 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 1 285 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 1,118 Reads with adapters: 2 (0.2%) Reads written (passing filters): 1,118 (100.0%) Total basepairs processed: 262,292 bp Total written (filtered): 262,286 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 50.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 17.5 0 2