This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.39 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 134,079 (5.2%) Reads that were too short: 18,080 (0.7%) Reads written (passing filters): 115,999 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 27,942,224 bp (3.6%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 134079 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3687 628.5 0 3687 7 7168 628.5 0 7168 8 165 628.5 0 165 9 6232 628.5 0 6232 10 97 628.5 0 97 11 99 628.5 0 99 12 151 628.5 0 151 13 141 628.5 0 141 14 112 628.5 0 112 15 110 628.5 0 110 16 193 628.5 0 193 17 159 628.5 0 159 18 150 628.5 0 150 19 116 628.5 0 116 20 177 628.5 0 177 21 163 628.5 0 163 22 140 628.5 0 140 23 178 628.5 0 178 24 176 628.5 0 176 25 158 628.5 0 158 26 141 628.5 0 141 27 163 628.5 0 163 28 197 628.5 0 197 29 251 628.5 0 251 30 211 628.5 0 211 31 196 628.5 0 196 32 351 628.5 0 351 33 236 628.5 0 236 34 167 628.5 0 167 35 195 628.5 0 195 36 200 628.5 0 200 37 193 628.5 0 193 38 290 628.5 0 290 39 265 628.5 0 265 40 302 628.5 0 302 41 300 628.5 0 300 42 5704 628.5 0 5704 43 6592 628.5 0 6592 44 4522 628.5 0 4522 45 165 628.5 0 165 46 170 628.5 0 170 47 219 628.5 0 219 48 313 628.5 0 313 49 194 628.5 0 194 50 192 628.5 0 192 51 190 628.5 0 190 52 174 628.5 0 174 53 230 628.5 0 230 54 188 628.5 0 188 55 206 628.5 0 206 56 139 628.5 0 139 57 206 628.5 0 206 58 224 628.5 0 224 59 203 628.5 0 203 60 271 628.5 0 271 61 429 628.5 0 429 62 540 628.5 0 540 63 847 628.5 0 847 64 1347 628.5 0 1347 65 18068 628.5 0 18068 66 10526 628.5 0 10526 67 25376 628.5 0 25376 68 221 628.5 0 221 69 142 628.5 0 142 70 143 628.5 0 143 71 132 628.5 0 132 72 150 628.5 0 150 73 138 628.5 0 138 74 118 628.5 0 118 75 105 628.5 0 105 76 119 628.5 0 119 77 151 628.5 0 151 78 127 628.5 0 127 79 145 628.5 0 145 80 129 628.5 0 129 81 134 628.5 0 134 82 133 628.5 0 133 83 134 628.5 0 134 84 152 628.5 0 152 85 132 628.5 0 132 86 118 628.5 0 118 87 167 628.5 0 167 88 127 628.5 0 127 89 132 628.5 0 132 90 121 628.5 0 121 91 124 628.5 0 124 92 127 628.5 0 127 93 132 628.5 0 132 94 131 628.5 0 131 95 184 628.5 0 184 96 122 628.5 0 122 97 125 628.5 0 125 98 148 628.5 0 148 99 156 628.5 0 156 100 139 628.5 0 139 101 144 628.5 0 144 102 155 628.5 0 155 103 125 628.5 0 125 104 130 628.5 0 130 105 125 628.5 0 125 106 137 628.5 0 137 107 128 628.5 0 128 108 143 628.5 0 143 109 110 628.5 0 110 110 131 628.5 0 131 111 129 628.5 0 129 112 147 628.5 0 147 113 123 628.5 0 123 114 110 628.5 0 110 115 117 628.5 0 117 116 107 628.5 0 107 117 100 628.5 0 100 118 124 628.5 0 124 119 88 628.5 0 88 120 97 628.5 0 97 121 132 628.5 0 132 122 86 628.5 0 86 123 117 628.5 0 117 124 125 628.5 0 125 125 118 628.5 0 118 126 129 628.5 0 129 127 111 628.5 0 111 128 95 628.5 0 95 129 95 628.5 0 95 130 103 628.5 0 103 131 114 628.5 0 114 132 139 628.5 0 139 133 125 628.5 0 125 134 172 628.5 0 172 135 155 628.5 0 155 136 151 628.5 0 151 137 112 628.5 0 112 138 146 628.5 0 146 139 125 628.5 0 125 140 131 628.5 0 131 141 108 628.5 0 108 142 104 628.5 0 104 143 87 628.5 0 87 144 95 628.5 0 95 145 95 628.5 0 95 146 115 628.5 0 115 147 87 628.5 0 87 148 99 628.5 0 99 149 106 628.5 0 106 150 81 628.5 0 81 151 86 628.5 0 86 152 86 628.5 0 86 153 87 628.5 0 87 154 102 628.5 0 102 155 73 628.5 0 73 156 86 628.5 0 86 157 84 628.5 0 84 158 71 628.5 0 71 159 108 628.5 0 108 160 86 628.5 0 86 161 94 628.5 0 94 162 102 628.5 0 102 163 99 628.5 0 99 164 102 628.5 0 102 165 71 628.5 0 71 166 94 628.5 0 94 167 87 628.5 0 87 168 116 628.5 0 116 169 100 628.5 0 100 170 115 628.5 0 115 171 90 628.5 0 90 172 107 628.5 0 107 173 105 628.5 0 105 174 120 628.5 0 120 175 101 628.5 0 101 176 74 628.5 0 74 177 96 628.5 0 96 178 112 628.5 0 112 179 130 628.5 0 130 180 106 628.5 0 106 181 119 628.5 0 119 182 82 628.5 0 82 183 89 628.5 0 89 184 102 628.5 0 102 185 111 628.5 0 111 186 111 628.5 0 111 187 76 628.5 0 76 188 104 628.5 0 104 189 106 628.5 0 106 190 90 628.5 0 90 191 130 628.5 0 130 192 86 628.5 0 86 193 82 628.5 0 82 194 98 628.5 0 98 195 87 628.5 0 87 196 82 628.5 0 82 197 134 628.5 0 134 198 110 628.5 0 110 199 111 628.5 0 111 200 142 628.5 0 142 201 106 628.5 0 106 202 84 628.5 0 84 203 111 628.5 0 111 204 87 628.5 0 87 205 90 628.5 0 90 206 79 628.5 0 79 207 76 628.5 0 76 208 77 628.5 0 77 209 109 628.5 0 109 210 91 628.5 0 91 211 98 628.5 0 98 212 79 628.5 0 79 213 68 628.5 0 68 214 80 628.5 0 80 215 91 628.5 0 91 216 120 628.5 0 120 217 108 628.5 0 108 218 114 628.5 0 114 219 110 628.5 0 110 220 134 628.5 0 134 221 89 628.5 0 89 222 237 628.5 0 237 223 182 628.5 0 182 224 328 628.5 0 328 225 290 628.5 0 290 226 106 628.5 0 106 227 96 628.5 0 96 228 113 628.5 0 113 229 92 628.5 0 92 230 105 628.5 0 105 231 76 628.5 0 76 232 79 628.5 0 79 233 93 628.5 0 93 234 85 628.5 0 85 235 97 628.5 0 97 236 133 628.5 0 133 237 110 628.5 0 110 238 99 628.5 0 99 239 79 628.5 0 79 240 109 628.5 0 109 241 98 628.5 0 98 242 214 628.5 0 214 243 202 628.5 0 202 244 146 628.5 0 146 245 163 628.5 0 163 246 126 628.5 0 126 247 133 628.5 0 133 248 89 628.5 0 89 249 109 628.5 0 109 250 139 628.5 0 139 251 143 628.5 0 143 252 139 628.5 0 139 253 219 628.5 0 219 254 284 628.5 0 284 255 1714 628.5 0 1714 256 498 628.5 0 498 257 1131 628.5 0 1131 258 201 628.5 0 201 259 133 628.5 0 133 260 997 628.5 0 997 261 1632 628.5 0 1632 262 553 628.5 0 553 263 259 628.5 0 259 264 126 628.5 0 126 265 121 628.5 0 121 266 171 628.5 0 171 267 97 628.5 0 97 268 77 628.5 0 77 269 102 628.5 0 102 270 272 628.5 0 272 271 290 628.5 0 290 272 393 628.5 0 393 273 99 628.5 0 99 274 81 628.5 0 81 275 91 628.5 0 91 276 85 628.5 0 85 277 99 628.5 0 99 278 119 628.5 0 119 279 113 628.5 0 113 280 86 628.5 0 86 281 64 628.5 0 64 282 69 628.5 0 69 283 53 628.5 0 53 284 91 628.5 0 91 285 67 628.5 0 67 286 69 628.5 0 69 287 76 628.5 0 76 288 66 628.5 0 66 289 68 628.5 0 68 290 79 628.5 0 79 291 89 628.5 0 89 292 120 628.5 0 120 293 126 628.5 0 126 294 223 628.5 0 223 295 68 628.5 0 68 296 87 628.5 0 87 297 66 628.5 0 66 298 84 628.5 0 84 299 147 628.5 0 147 300 107 628.5 0 107 301 113 628.5 0 113 Finished in 5.41 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 115,999 Reads with adapters: 9,646 (8.3%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 9,608 (8.3%) Total basepairs processed: 27,942,224 bp Total written (filtered): 2,589,001 bp (9.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9646 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 85 0.0 3 0 84 1 20 6960 0.0 4 6851 108 1 21 14 0.0 4 4 10 22 25 0.0 4 7 17 1 23 2164 0.0 4 2122 35 2 3 2 24 6 0.0 4 1 4 0 0 1 25 1 0.0 4 0 0 1 26 1 0.0 4 0 0 0 1 34 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 1 92 17 0.0 4 0 0 0 0 17 107 10 0.0 4 0 0 0 0 10 113 11 0.0 4 0 0 0 0 11 114 6 0.0 4 0 0 0 0 6 115 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 287 0.0 4 0 0 0 0 287 128 2 0.0 4 0 0 0 0 2 130 13 0.0 4 0 0 0 0 13 144 1 0.0 4 0 0 0 0 1 154 1 0.0 4 1 167 1 0.0 4 0 0 0 0 1 193 18 0.0 4 0 0 0 0 18 194 12 0.0 4 0 0 0 0 12 195 1 0.0 4 0 0 0 1 201 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 9,608 Reads with adapters: 497 (5.2%) Reads written (passing filters): 9,608 (100.0%) Total basepairs processed: 2,589,001 bp Total written (filtered): 2,580,170 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 497 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.4% C: 6.0% G: 87.7% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 150.1 0 15 4 2 37.5 0 2 5 1 9.4 0 1 9 153 0.0 0 5 148 10 135 0.0 1 9 126 11 148 0.0 1 1 147 12 23 0.0 1 2 21 23 1 0.0 1 1 124 2 0.0 1 2 151 1 0.0 1 0 1 162 1 0.0 1 1 171 2 0.0 1 2 210 1 0.0 1 1 223 3 0.0 1 3 224 1 0.0 1 1 264 7 0.0 1 7 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.