This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.43 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 40,006 (1.6%) Reads that were too short: 17,292 (0.7%) Reads written (passing filters): 22,714 (0.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 4,561,098 bp (0.6%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 40006 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 133 628.5 0 133 7 2206 628.5 0 2206 8 1931 628.5 0 1931 9 49 628.5 0 49 10 54 628.5 0 54 11 63 628.5 0 63 12 60 628.5 0 60 13 39 628.5 0 39 14 74 628.5 0 74 15 37 628.5 0 37 16 39 628.5 0 39 17 30 628.5 0 30 18 38 628.5 0 38 19 45 628.5 0 45 20 55 628.5 0 55 21 77 628.5 0 77 22 93 628.5 0 93 23 42 628.5 0 42 24 49 628.5 0 49 25 35 628.5 0 35 26 34 628.5 0 34 27 33 628.5 0 33 28 24 628.5 0 24 29 40 628.5 0 40 30 59 628.5 0 59 31 40 628.5 0 40 32 44 628.5 0 44 33 34 628.5 0 34 34 40 628.5 0 40 35 47 628.5 0 47 36 68 628.5 0 68 37 69 628.5 0 69 38 70 628.5 0 70 39 78 628.5 0 78 40 101 628.5 0 101 41 66 628.5 0 66 42 41 628.5 0 41 43 44 628.5 0 44 44 43 628.5 0 43 45 43 628.5 0 43 46 40 628.5 0 40 47 35 628.5 0 35 48 163 628.5 0 163 49 79 628.5 0 79 50 38 628.5 0 38 51 70 628.5 0 70 52 54 628.5 0 54 53 47 628.5 0 47 54 45 628.5 0 45 55 55 628.5 0 55 56 44 628.5 0 44 57 53 628.5 0 53 58 43 628.5 0 43 59 41 628.5 0 41 60 98 628.5 0 98 61 108 628.5 0 108 62 80 628.5 0 80 63 40 628.5 0 40 64 55 628.5 0 55 65 58 628.5 0 58 66 48 628.5 0 48 67 62 628.5 0 62 68 67 628.5 0 67 69 295 628.5 0 295 70 412 628.5 0 412 71 511 628.5 0 511 72 256 628.5 0 256 73 78 628.5 0 78 74 354 628.5 0 354 75 47 628.5 0 47 76 41 628.5 0 41 77 45 628.5 0 45 78 45 628.5 0 45 79 59 628.5 0 59 80 124 628.5 0 124 81 125 628.5 0 125 82 105 628.5 0 105 83 108 628.5 0 108 84 364 628.5 0 364 85 177 628.5 0 177 86 227 628.5 0 227 87 61 628.5 0 61 88 70 628.5 0 70 89 48 628.5 0 48 90 64 628.5 0 64 91 60 628.5 0 60 92 50 628.5 0 50 93 43 628.5 0 43 94 46 628.5 0 46 95 49 628.5 0 49 96 45 628.5 0 45 97 61 628.5 0 61 98 51 628.5 0 51 99 71 628.5 0 71 100 54 628.5 0 54 101 89 628.5 0 89 102 66 628.5 0 66 103 44 628.5 0 44 104 44 628.5 0 44 105 35 628.5 0 35 106 40 628.5 0 40 107 51 628.5 0 51 108 50 628.5 0 50 109 47 628.5 0 47 110 115 628.5 0 115 111 173 628.5 0 173 112 295 628.5 0 295 113 53 628.5 0 53 114 45 628.5 0 45 115 40 628.5 0 40 116 41 628.5 0 41 117 47 628.5 0 47 118 29 628.5 0 29 119 53 628.5 0 53 120 44 628.5 0 44 121 50 628.5 0 50 122 28 628.5 0 28 123 67 628.5 0 67 124 69 628.5 0 69 125 62 628.5 0 62 126 44 628.5 0 44 127 45 628.5 0 45 128 46 628.5 0 46 129 35 628.5 0 35 130 40 628.5 0 40 131 73 628.5 0 73 132 273 628.5 0 273 133 54 628.5 0 54 134 48 628.5 0 48 135 38 628.5 0 38 136 41 628.5 0 41 137 42 628.5 0 42 138 64 628.5 0 64 139 51 628.5 0 51 140 45 628.5 0 45 141 53 628.5 0 53 142 65 628.5 0 65 143 60 628.5 0 60 144 52 628.5 0 52 145 94 628.5 0 94 146 131 628.5 0 131 147 155 628.5 0 155 148 240 628.5 0 240 149 166 628.5 0 166 150 221 628.5 0 221 151 130 628.5 0 130 152 100 628.5 0 100 153 143 628.5 0 143 154 63 628.5 0 63 155 62 628.5 0 62 156 178 628.5 0 178 157 131 628.5 0 131 158 167 628.5 0 167 159 98 628.5 0 98 160 37 628.5 0 37 161 68 628.5 0 68 162 38 628.5 0 38 163 49 628.5 0 49 164 50 628.5 0 50 165 53 628.5 0 53 166 46 628.5 0 46 167 65 628.5 0 65 168 101 628.5 0 101 169 121 628.5 0 121 170 192 628.5 0 192 171 59 628.5 0 59 172 51 628.5 0 51 173 55 628.5 0 55 174 70 628.5 0 70 175 77 628.5 0 77 176 390 628.5 0 390 177 364 628.5 0 364 178 542 628.5 0 542 179 52 628.5 0 52 180 49 628.5 0 49 181 47 628.5 0 47 182 102 628.5 0 102 183 138 628.5 0 138 184 88 628.5 0 88 185 44 628.5 0 44 186 62 628.5 0 62 187 132 628.5 0 132 188 202 628.5 0 202 189 273 628.5 0 273 190 105 628.5 0 105 191 71 628.5 0 71 192 53 628.5 0 53 193 50 628.5 0 50 194 65 628.5 0 65 195 105 628.5 0 105 196 185 628.5 0 185 197 395 628.5 0 395 198 695 628.5 0 695 199 500 628.5 0 500 200 96 628.5 0 96 201 116 628.5 0 116 202 421 628.5 0 421 203 758 628.5 0 758 204 792 628.5 0 792 205 376 628.5 0 376 206 193 628.5 0 193 207 58 628.5 0 58 208 47 628.5 0 47 209 84 628.5 0 84 210 109 628.5 0 109 211 91 628.5 0 91 212 123 628.5 0 123 213 209 628.5 0 209 214 233 628.5 0 233 215 180 628.5 0 180 216 169 628.5 0 169 217 139 628.5 0 139 218 130 628.5 0 130 219 145 628.5 0 145 220 98 628.5 0 98 221 98 628.5 0 98 222 247 628.5 0 247 223 877 628.5 0 877 224 1103 628.5 0 1103 225 815 628.5 0 815 226 63 628.5 0 63 227 67 628.5 0 67 228 80 628.5 0 80 229 65 628.5 0 65 230 49 628.5 0 49 231 48 628.5 0 48 232 41 628.5 0 41 233 61 628.5 0 61 234 97 628.5 0 97 235 94 628.5 0 94 236 78 628.5 0 78 237 65 628.5 0 65 238 83 628.5 0 83 239 101 628.5 0 101 240 93 628.5 0 93 241 182 628.5 0 182 242 160 628.5 0 160 243 154 628.5 0 154 244 127 628.5 0 127 245 152 628.5 0 152 246 137 628.5 0 137 247 73 628.5 0 73 248 83 628.5 0 83 249 88 628.5 0 88 250 63 628.5 0 63 251 77 628.5 0 77 252 53 628.5 0 53 253 55 628.5 0 55 254 92 628.5 0 92 255 75 628.5 0 75 256 74 628.5 0 74 257 97 628.5 0 97 258 271 628.5 0 271 259 522 628.5 0 522 260 720 628.5 0 720 261 86 628.5 0 86 262 85 628.5 0 85 263 65 628.5 0 65 264 84 628.5 0 84 265 57 628.5 0 57 266 64 628.5 0 64 267 66 628.5 0 66 268 45 628.5 0 45 269 56 628.5 0 56 270 93 628.5 0 93 271 250 628.5 0 250 272 182 628.5 0 182 273 181 628.5 0 181 274 59 628.5 0 59 275 60 628.5 0 60 276 58 628.5 0 58 277 97 628.5 0 97 278 108 628.5 0 108 279 79 628.5 0 79 280 99 628.5 0 99 281 88 628.5 0 88 282 229 628.5 0 229 283 209 628.5 0 209 284 271 628.5 0 271 285 182 628.5 0 182 286 114 628.5 0 114 287 117 628.5 0 117 288 78 628.5 0 78 289 87 628.5 0 87 290 58 628.5 0 58 291 74 628.5 0 74 292 79 628.5 0 79 293 99 628.5 0 99 294 111 628.5 0 111 295 110 628.5 0 110 296 133 628.5 0 133 297 286 628.5 0 286 298 410 628.5 0 410 299 425 628.5 0 425 300 257 628.5 0 257 301 166 628.5 0 166 Finished in 0.92 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 22,714 Reads with adapters: 2,246 (9.9%) Reads that were too short: 84 (0.4%) Reads written (passing filters): 2,162 (9.5%) Total basepairs processed: 4,561,098 bp Total written (filtered): 587,033 bp (12.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2246 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 23 0.0 3 0 16 4 3 17 2034 0.0 3 1827 174 27 6 18 11 0.0 3 1 6 4 19 10 0.0 3 3 0 4 3 20 9 0.0 3 2 0 2 5 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 29 5 0.0 3 0 0 0 5 31 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 38 3 0.0 3 0 0 0 3 41 17 0.0 3 0 0 0 17 42 2 0.0 3 0 0 0 2 43 3 0.0 3 0 0 0 3 44 6 0.0 3 0 0 2 4 49 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 4 0.0 3 0 0 0 4 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 79 4 0.0 3 0 0 0 4 80 3 0.0 3 0 0 0 3 81 3 0.0 3 0 0 1 2 82 2 0.0 3 0 0 0 2 83 2 0.0 3 0 0 0 2 84 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 127 1 0.0 3 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 1 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 1 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 217 3 0.0 3 0 0 1 2 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 2,162 Reads with adapters: 2 (0.1%) Reads written (passing filters): 2,162 (100.0%) Total basepairs processed: 587,033 bp Total written (filtered): 587,026 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 33.8 0 1 4 1 8.4 0 1