This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.12 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 25,549 (1.0%) Reads that were too short: 7,914 (0.3%) Reads written (passing filters): 17,635 (0.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 3,885,703 bp (0.5%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 25549 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 628.5 0 129 7 2125 628.5 0 2125 8 2313 628.5 0 2313 9 50 628.5 0 50 10 39 628.5 0 39 11 80 628.5 0 80 12 37 628.5 0 37 13 41 628.5 0 41 14 61 628.5 0 61 15 44 628.5 0 44 16 53 628.5 0 53 17 39 628.5 0 39 18 27 628.5 0 27 19 50 628.5 0 50 20 41 628.5 0 41 21 45 628.5 0 45 22 55 628.5 0 55 23 55 628.5 0 55 24 32 628.5 0 32 25 40 628.5 0 40 26 30 628.5 0 30 27 39 628.5 0 39 28 47 628.5 0 47 29 49 628.5 0 49 30 54 628.5 0 54 31 47 628.5 0 47 32 44 628.5 0 44 33 43 628.5 0 43 34 44 628.5 0 44 35 27 628.5 0 27 36 44 628.5 0 44 37 43 628.5 0 43 38 32 628.5 0 32 39 38 628.5 0 38 40 46 628.5 0 46 41 51 628.5 0 51 42 43 628.5 0 43 43 54 628.5 0 54 44 34 628.5 0 34 45 47 628.5 0 47 46 42 628.5 0 42 47 30 628.5 0 30 48 64 628.5 0 64 49 71 628.5 0 71 50 53 628.5 0 53 51 42 628.5 0 42 52 38 628.5 0 38 53 42 628.5 0 42 54 48 628.5 0 48 55 64 628.5 0 64 56 70 628.5 0 70 57 59 628.5 0 59 58 43 628.5 0 43 59 34 628.5 0 34 60 68 628.5 0 68 61 42 628.5 0 42 62 46 628.5 0 46 63 48 628.5 0 48 64 35 628.5 0 35 65 51 628.5 0 51 66 46 628.5 0 46 67 58 628.5 0 58 68 63 628.5 0 63 69 261 628.5 0 261 70 516 628.5 0 516 71 672 628.5 0 672 72 306 628.5 0 306 73 84 628.5 0 84 74 352 628.5 0 352 75 53 628.5 0 53 76 43 628.5 0 43 77 33 628.5 0 33 78 45 628.5 0 45 79 48 628.5 0 48 80 70 628.5 0 70 81 72 628.5 0 72 82 82 628.5 0 82 83 52 628.5 0 52 84 329 628.5 0 329 85 176 628.5 0 176 86 251 628.5 0 251 87 32 628.5 0 32 88 45 628.5 0 45 89 45 628.5 0 45 90 39 628.5 0 39 91 37 628.5 0 37 92 33 628.5 0 33 93 44 628.5 0 44 94 70 628.5 0 70 95 39 628.5 0 39 96 35 628.5 0 35 97 46 628.5 0 46 98 33 628.5 0 33 99 56 628.5 0 56 100 41 628.5 0 41 101 44 628.5 0 44 102 49 628.5 0 49 103 26 628.5 0 26 104 32 628.5 0 32 105 31 628.5 0 31 106 35 628.5 0 35 107 54 628.5 0 54 108 44 628.5 0 44 109 39 628.5 0 39 110 85 628.5 0 85 111 146 628.5 0 146 112 230 628.5 0 230 113 48 628.5 0 48 114 46 628.5 0 46 115 35 628.5 0 35 116 29 628.5 0 29 117 43 628.5 0 43 118 26 628.5 0 26 119 29 628.5 0 29 120 48 628.5 0 48 121 65 628.5 0 65 122 27 628.5 0 27 123 28 628.5 0 28 124 53 628.5 0 53 125 54 628.5 0 54 126 40 628.5 0 40 127 25 628.5 0 25 128 49 628.5 0 49 129 41 628.5 0 41 130 31 628.5 0 31 131 69 628.5 0 69 132 230 628.5 0 230 133 42 628.5 0 42 134 43 628.5 0 43 135 36 628.5 0 36 136 26 628.5 0 26 137 36 628.5 0 36 138 49 628.5 0 49 139 42 628.5 0 42 140 33 628.5 0 33 141 39 628.5 0 39 142 35 628.5 0 35 143 36 628.5 0 36 144 40 628.5 0 40 145 56 628.5 0 56 146 54 628.5 0 54 147 70 628.5 0 70 148 168 628.5 0 168 149 89 628.5 0 89 150 139 628.5 0 139 151 45 628.5 0 45 152 31 628.5 0 31 153 48 628.5 0 48 154 36 628.5 0 36 155 41 628.5 0 41 156 82 628.5 0 82 157 54 628.5 0 54 158 81 628.5 0 81 159 67 628.5 0 67 160 33 628.5 0 33 161 79 628.5 0 79 162 36 628.5 0 36 163 27 628.5 0 27 164 29 628.5 0 29 165 36 628.5 0 36 166 44 628.5 0 44 167 38 628.5 0 38 168 91 628.5 0 91 169 80 628.5 0 80 170 160 628.5 0 160 171 49 628.5 0 49 172 55 628.5 0 55 173 39 628.5 0 39 174 47 628.5 0 47 175 42 628.5 0 42 176 122 628.5 0 122 177 131 628.5 0 131 178 107 628.5 0 107 179 35 628.5 0 35 180 53 628.5 0 53 181 41 628.5 0 41 182 110 628.5 0 110 183 119 628.5 0 119 184 62 628.5 0 62 185 50 628.5 0 50 186 53 628.5 0 53 187 67 628.5 0 67 188 78 628.5 0 78 189 75 628.5 0 75 190 42 628.5 0 42 191 42 628.5 0 42 192 46 628.5 0 46 193 38 628.5 0 38 194 35 628.5 0 35 195 48 628.5 0 48 196 81 628.5 0 81 197 130 628.5 0 130 198 156 628.5 0 156 199 194 628.5 0 194 200 45 628.5 0 45 201 32 628.5 0 32 202 118 628.5 0 118 203 243 628.5 0 243 204 225 628.5 0 225 205 138 628.5 0 138 206 78 628.5 0 78 207 39 628.5 0 39 208 51 628.5 0 51 209 47 628.5 0 47 210 57 628.5 0 57 211 47 628.5 0 47 212 43 628.5 0 43 213 58 628.5 0 58 214 66 628.5 0 66 215 82 628.5 0 82 216 59 628.5 0 59 217 47 628.5 0 47 218 54 628.5 0 54 219 52 628.5 0 52 220 65 628.5 0 65 221 42 628.5 0 42 222 74 628.5 0 74 223 226 628.5 0 226 224 359 628.5 0 359 225 279 628.5 0 279 226 43 628.5 0 43 227 50 628.5 0 50 228 64 628.5 0 64 229 38 628.5 0 38 230 42 628.5 0 42 231 39 628.5 0 39 232 45 628.5 0 45 233 51 628.5 0 51 234 47 628.5 0 47 235 62 628.5 0 62 236 62 628.5 0 62 237 44 628.5 0 44 238 46 628.5 0 46 239 62 628.5 0 62 240 57 628.5 0 57 241 72 628.5 0 72 242 68 628.5 0 68 243 55 628.5 0 55 244 75 628.5 0 75 245 79 628.5 0 79 246 63 628.5 0 63 247 55 628.5 0 55 248 45 628.5 0 45 249 63 628.5 0 63 250 62 628.5 0 62 251 50 628.5 0 50 252 31 628.5 0 31 253 42 628.5 0 42 254 62 628.5 0 62 255 57 628.5 0 57 256 48 628.5 0 48 257 56 628.5 0 56 258 147 628.5 0 147 259 262 628.5 0 262 260 425 628.5 0 425 261 84 628.5 0 84 262 37 628.5 0 37 263 42 628.5 0 42 264 41 628.5 0 41 265 35 628.5 0 35 266 43 628.5 0 43 267 38 628.5 0 38 268 44 628.5 0 44 269 46 628.5 0 46 270 53 628.5 0 53 271 113 628.5 0 113 272 86 628.5 0 86 273 92 628.5 0 92 274 42 628.5 0 42 275 46 628.5 0 46 276 50 628.5 0 50 277 57 628.5 0 57 278 64 628.5 0 64 279 61 628.5 0 61 280 60 628.5 0 60 281 58 628.5 0 58 282 99 628.5 0 99 283 119 628.5 0 119 284 115 628.5 0 115 285 94 628.5 0 94 286 57 628.5 0 57 287 87 628.5 0 87 288 51 628.5 0 51 289 51 628.5 0 51 290 42 628.5 0 42 291 53 628.5 0 53 292 35 628.5 0 35 293 48 628.5 0 48 294 67 628.5 0 67 295 47 628.5 0 47 296 53 628.5 0 53 297 112 628.5 0 112 298 170 628.5 0 170 299 154 628.5 0 154 300 87 628.5 0 87 301 63 628.5 0 63 Finished in 0.83 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 17,635 Reads with adapters: 2,495 (14.1%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 2,494 (14.1%) Total basepairs processed: 3,885,703 bp Total written (filtered): 670,268 bp (17.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2495 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 40 0.0 3 0 40 20 2345 0.0 4 2303 42 21 5 0.0 4 1 4 22 1 0.0 4 1 37 1 0.0 4 1 63 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 1 106 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 99 0.0 4 0 0 0 0 99 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 2,494 Reads with adapters: 296 (11.9%) Reads written (passing filters): 2,494 (100.0%) Total basepairs processed: 670,268 bp Total written (filtered): 666,306 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 296 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 2.7% G: 91.6% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 39.0 0 2 9 1 0.0 0 0 1 10 23 0.0 1 0 23 11 260 0.0 1 3 257 12 1 0.0 1 0 1 19 5 0.0 1 4 1 21 1 0.0 1 1 209 1 0.0 1 1 260 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.