This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.63 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 141,412 (5.5%) Reads that were too short: 26,780 (1.0%) Reads written (passing filters): 114,632 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 27,445,765 bp (3.5%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 141412 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4594 628.5 0 4594 7 31445 628.5 0 31445 8 432 628.5 0 432 9 31581 628.5 0 31581 10 38 628.5 0 38 11 52 628.5 0 52 12 60 628.5 0 60 13 36 628.5 0 36 14 24 628.5 0 24 15 28 628.5 0 28 16 43 628.5 0 43 17 61 628.5 0 61 18 48 628.5 0 48 19 42 628.5 0 42 20 31 628.5 0 31 21 39 628.5 0 39 22 34 628.5 0 34 23 39 628.5 0 39 24 37 628.5 0 37 25 42 628.5 0 42 26 50 628.5 0 50 27 37 628.5 0 37 28 38 628.5 0 38 29 43 628.5 0 43 30 36 628.5 0 36 31 41 628.5 0 41 32 39 628.5 0 39 33 39 628.5 0 39 34 51 628.5 0 51 35 37 628.5 0 37 36 26 628.5 0 26 37 21 628.5 0 21 38 45 628.5 0 45 39 45 628.5 0 45 40 49 628.5 0 49 41 32 628.5 0 32 42 42 628.5 0 42 43 52 628.5 0 52 44 62 628.5 0 62 45 33 628.5 0 33 46 36 628.5 0 36 47 54 628.5 0 54 48 28 628.5 0 28 49 40 628.5 0 40 50 44 628.5 0 44 51 44 628.5 0 44 52 33 628.5 0 33 53 43 628.5 0 43 54 34 628.5 0 34 55 41 628.5 0 41 56 65 628.5 0 65 57 62 628.5 0 62 58 54 628.5 0 54 59 53 628.5 0 53 60 48 628.5 0 48 61 35 628.5 0 35 62 53 628.5 0 53 63 54 628.5 0 54 64 49 628.5 0 49 65 35 628.5 0 35 66 40 628.5 0 40 67 44 628.5 0 44 68 49 628.5 0 49 69 41 628.5 0 41 70 33 628.5 0 33 71 80 628.5 0 80 72 68 628.5 0 68 73 152 628.5 0 152 74 60 628.5 0 60 75 47 628.5 0 47 76 45 628.5 0 45 77 52 628.5 0 52 78 47 628.5 0 47 79 43 628.5 0 43 80 41 628.5 0 41 81 34 628.5 0 34 82 68 628.5 0 68 83 41 628.5 0 41 84 50 628.5 0 50 85 68 628.5 0 68 86 58 628.5 0 58 87 106 628.5 0 106 88 61 628.5 0 61 89 119 628.5 0 119 90 82 628.5 0 82 91 92 628.5 0 92 92 58 628.5 0 58 93 52 628.5 0 52 94 34 628.5 0 34 95 43 628.5 0 43 96 45 628.5 0 45 97 64 628.5 0 64 98 71 628.5 0 71 99 53 628.5 0 53 100 45 628.5 0 45 101 60 628.5 0 60 102 61 628.5 0 61 103 158 628.5 0 158 104 189 628.5 0 189 105 144 628.5 0 144 106 125 628.5 0 125 107 74 628.5 0 74 108 51 628.5 0 51 109 60 628.5 0 60 110 47 628.5 0 47 111 40 628.5 0 40 112 50 628.5 0 50 113 63 628.5 0 63 114 43 628.5 0 43 115 40 628.5 0 40 116 75 628.5 0 75 117 42 628.5 0 42 118 60 628.5 0 60 119 79 628.5 0 79 120 46 628.5 0 46 121 65 628.5 0 65 122 53 628.5 0 53 123 83 628.5 0 83 124 56 628.5 0 56 125 68 628.5 0 68 126 64 628.5 0 64 127 78 628.5 0 78 128 109 628.5 0 109 129 175 628.5 0 175 130 52 628.5 0 52 131 89 628.5 0 89 132 118 628.5 0 118 133 101 628.5 0 101 134 164 628.5 0 164 135 114 628.5 0 114 136 89 628.5 0 89 137 91 628.5 0 91 138 122 628.5 0 122 139 118 628.5 0 118 140 223 628.5 0 223 141 180 628.5 0 180 142 240 628.5 0 240 143 289 628.5 0 289 144 336 628.5 0 336 145 430 628.5 0 430 146 5519 628.5 0 5519 147 4084 628.5 0 4084 148 10786 628.5 0 10786 149 320 628.5 0 320 150 3953 628.5 0 3953 151 1934 628.5 0 1934 152 5732 628.5 0 5732 153 95 628.5 0 95 154 87 628.5 0 87 155 98 628.5 0 98 156 1126 628.5 0 1126 157 175 628.5 0 175 158 474 628.5 0 474 159 60 628.5 0 60 160 139 628.5 0 139 161 102 628.5 0 102 162 128 628.5 0 128 163 60 628.5 0 60 164 48 628.5 0 48 165 26 628.5 0 26 166 60 628.5 0 60 167 50 628.5 0 50 168 52 628.5 0 52 169 37 628.5 0 37 170 41 628.5 0 41 171 42 628.5 0 42 172 30 628.5 0 30 173 33 628.5 0 33 174 47 628.5 0 47 175 91 628.5 0 91 176 83 628.5 0 83 177 77 628.5 0 77 178 44 628.5 0 44 179 45 628.5 0 45 180 53 628.5 0 53 181 110 628.5 0 110 182 91 628.5 0 91 183 97 628.5 0 97 184 62 628.5 0 62 185 65 628.5 0 65 186 99 628.5 0 99 187 81 628.5 0 81 188 96 628.5 0 96 189 87 628.5 0 87 190 61 628.5 0 61 191 36 628.5 0 36 192 44 628.5 0 44 193 51 628.5 0 51 194 37 628.5 0 37 195 52 628.5 0 52 196 56 628.5 0 56 197 51 628.5 0 51 198 39 628.5 0 39 199 46 628.5 0 46 200 51 628.5 0 51 201 60 628.5 0 60 202 70 628.5 0 70 203 63 628.5 0 63 204 83 628.5 0 83 205 70 628.5 0 70 206 90 628.5 0 90 207 65 628.5 0 65 208 106 628.5 0 106 209 74 628.5 0 74 210 65 628.5 0 65 211 106 628.5 0 106 212 228 628.5 0 228 213 160 628.5 0 160 214 150 628.5 0 150 215 70 628.5 0 70 216 54 628.5 0 54 217 36 628.5 0 36 218 47 628.5 0 47 219 78 628.5 0 78 220 151 628.5 0 151 221 92 628.5 0 92 222 112 628.5 0 112 223 102 628.5 0 102 224 104 628.5 0 104 225 93 628.5 0 93 226 119 628.5 0 119 227 69 628.5 0 69 228 68 628.5 0 68 229 74 628.5 0 74 230 78 628.5 0 78 231 90 628.5 0 90 232 70 628.5 0 70 233 71 628.5 0 71 234 82 628.5 0 82 235 93 628.5 0 93 236 193 628.5 0 193 237 110 628.5 0 110 238 137 628.5 0 137 239 97 628.5 0 97 240 70 628.5 0 70 241 70 628.5 0 70 242 94 628.5 0 94 243 81 628.5 0 81 244 119 628.5 0 119 245 80 628.5 0 80 246 92 628.5 0 92 247 72 628.5 0 72 248 81 628.5 0 81 249 89 628.5 0 89 250 105 628.5 0 105 251 140 628.5 0 140 252 162 628.5 0 162 253 214 628.5 0 214 254 331 628.5 0 331 255 446 628.5 0 446 256 4397 628.5 0 4397 257 4333 628.5 0 4333 258 6516 628.5 0 6516 259 1383 628.5 0 1383 260 95 628.5 0 95 261 100 628.5 0 100 262 121 628.5 0 121 263 142 628.5 0 142 264 122 628.5 0 122 265 87 628.5 0 87 266 75 628.5 0 75 267 118 628.5 0 118 268 119 628.5 0 119 269 170 628.5 0 170 270 125 628.5 0 125 271 54 628.5 0 54 272 39 628.5 0 39 273 66 628.5 0 66 274 77 628.5 0 77 275 76 628.5 0 76 276 75 628.5 0 75 277 69 628.5 0 69 278 69 628.5 0 69 279 109 628.5 0 109 280 232 628.5 0 232 281 254 628.5 0 254 282 281 628.5 0 281 283 118 628.5 0 118 284 101 628.5 0 101 285 104 628.5 0 104 286 78 628.5 0 78 287 80 628.5 0 80 288 80 628.5 0 80 289 102 628.5 0 102 290 61 628.5 0 61 291 53 628.5 0 53 292 40 628.5 0 40 293 88 628.5 0 88 294 63 628.5 0 63 295 88 628.5 0 88 296 151 628.5 0 151 297 77 628.5 0 77 298 75 628.5 0 75 299 52 628.5 0 52 300 87 628.5 0 87 301 112 628.5 0 112 Finished in 5.45 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 114,632 Reads with adapters: 37,889 (33.1%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 37,881 (33.0%) Total basepairs processed: 27,445,765 bp Total written (filtered): 10,136,390 bp (36.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37889 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 11 0.0 3 0 0 10 0 1 19 360 0.0 3 0 353 7 20 34934 0.0 4 34334 584 15 1 21 68 0.0 4 7 58 2 0 1 22 13 0.0 4 1 4 6 0 2 23 232 0.0 4 200 2 0 25 5 24 1 0.0 4 0 1 26 1 0.0 4 0 0 0 1 27 1 0.0 4 1 28 5 0.0 4 1 0 0 0 4 30 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 70 1 0.0 4 1 74 2 0.0 4 2 76 1 0.0 4 1 93 1 0.0 4 0 0 0 0 1 125 10 0.0 4 0 0 0 0 10 126 28 0.0 4 0 0 0 0 28 127 2203 0.0 4 0 0 0 0 2203 128 5 0.0 4 0 0 0 0 5 130 2 0.0 4 0 0 0 0 2 200 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 1 228 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.29 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 37,881 Reads with adapters: 840 (2.2%) Reads written (passing filters): 37,881 (100.0%) Total basepairs processed: 10,136,390 bp Total written (filtered): 10,076,175 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 840 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.1% C: 5.2% G: 58.9% T: 21.1% none/other: 3.7% Overview of removed sequences length count expect max.err error counts 3 104 591.9 0 104 4 10 148.0 0 10 5 2 37.0 0 2 6 1 9.2 0 1 9 18 0.1 0 1 17 10 18 0.0 1 0 18 11 347 0.0 1 5 342 12 134 0.0 1 0 134 13 3 0.0 1 0 3 15 1 0.0 1 0 1 16 2 0.0 1 0 2 125 1 0.0 1 0 1 171 2 0.0 1 2 225 1 0.0 1 1 266 1 0.0 1 1 267 3 0.0 1 2 1 270 1 0.0 1 0 1 271 101 0.0 1 100 1 272 15 0.0 1 14 1 273 40 0.0 1 37 3 274 31 0.0 1 27 4 275 4 0.0 1 4