This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.00 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 82,901 (3.2%) Reads that were too short: 23,326 (0.9%) Reads written (passing filters): 59,575 (2.3%) Total basepairs processed: 774,927,510 bp Total written (filtered): 10,152,640 bp (1.3%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 82901 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 413 628.5 0 413 7 4521 628.5 0 4521 8 303 628.5 0 303 9 4246 628.5 0 4246 10 40 628.5 0 40 11 29 628.5 0 29 12 31 628.5 0 31 13 37 628.5 0 37 14 36 628.5 0 36 15 65 628.5 0 65 16 28 628.5 0 28 17 32 628.5 0 32 18 31 628.5 0 31 19 30 628.5 0 30 20 31 628.5 0 31 21 47 628.5 0 47 22 43 628.5 0 43 23 44 628.5 0 44 24 39 628.5 0 39 25 36 628.5 0 36 26 19 628.5 0 19 27 44 628.5 0 44 28 48 628.5 0 48 29 28 628.5 0 28 30 45 628.5 0 45 31 32 628.5 0 32 32 35 628.5 0 35 33 32 628.5 0 32 34 25 628.5 0 25 35 77 628.5 0 77 36 42 628.5 0 42 37 28 628.5 0 28 38 67 628.5 0 67 39 46 628.5 0 46 40 194 628.5 0 194 41 106 628.5 0 106 42 167 628.5 0 167 43 32 628.5 0 32 44 37 628.5 0 37 45 35 628.5 0 35 46 35 628.5 0 35 47 49 628.5 0 49 48 27 628.5 0 27 49 38 628.5 0 38 50 38 628.5 0 38 51 43 628.5 0 43 52 40 628.5 0 40 53 50 628.5 0 50 54 34 628.5 0 34 55 248 628.5 0 248 56 135 628.5 0 135 57 323 628.5 0 323 58 42 628.5 0 42 59 53 628.5 0 53 60 81 628.5 0 81 61 46 628.5 0 46 62 45 628.5 0 45 63 42 628.5 0 42 64 59 628.5 0 59 65 47 628.5 0 47 66 90 628.5 0 90 67 53 628.5 0 53 68 83 628.5 0 83 69 37 628.5 0 37 70 41 628.5 0 41 71 68 628.5 0 68 72 47 628.5 0 47 73 65 628.5 0 65 74 63 628.5 0 63 75 78 628.5 0 78 76 44 628.5 0 44 77 44 628.5 0 44 78 46 628.5 0 46 79 47 628.5 0 47 80 23 628.5 0 23 81 24 628.5 0 24 82 44 628.5 0 44 83 41 628.5 0 41 84 90 628.5 0 90 85 34 628.5 0 34 86 30 628.5 0 30 87 43 628.5 0 43 88 43 628.5 0 43 89 64 628.5 0 64 90 102 628.5 0 102 91 69 628.5 0 69 92 47 628.5 0 47 93 50 628.5 0 50 94 39 628.5 0 39 95 61 628.5 0 61 96 38 628.5 0 38 97 39 628.5 0 39 98 26 628.5 0 26 99 23 628.5 0 23 100 19 628.5 0 19 101 55 628.5 0 55 102 74 628.5 0 74 103 99 628.5 0 99 104 79 628.5 0 79 105 304 628.5 0 304 106 508 628.5 0 508 107 293 628.5 0 293 108 24 628.5 0 24 109 37 628.5 0 37 110 49 628.5 0 49 111 56 628.5 0 56 112 91 628.5 0 91 113 102 628.5 0 102 114 109 628.5 0 109 115 262 628.5 0 262 116 82 628.5 0 82 117 92 628.5 0 92 118 71 628.5 0 71 119 38 628.5 0 38 120 43 628.5 0 43 121 62 628.5 0 62 122 50 628.5 0 50 123 46 628.5 0 46 124 52 628.5 0 52 125 67 628.5 0 67 126 117 628.5 0 117 127 232 628.5 0 232 128 81 628.5 0 81 129 144 628.5 0 144 130 158 628.5 0 158 131 82 628.5 0 82 132 159 628.5 0 159 133 106 628.5 0 106 134 161 628.5 0 161 135 84 628.5 0 84 136 88 628.5 0 88 137 98 628.5 0 98 138 82 628.5 0 82 139 78 628.5 0 78 140 114 628.5 0 114 141 105 628.5 0 105 142 159 628.5 0 159 143 143 628.5 0 143 144 229 628.5 0 229 145 224 628.5 0 224 146 310 628.5 0 310 147 4358 628.5 0 4358 148 7329 628.5 0 7329 149 4796 628.5 0 4796 150 821 628.5 0 821 151 115 628.5 0 115 152 258 628.5 0 258 153 250 628.5 0 250 154 592 628.5 0 592 155 125 628.5 0 125 156 166 628.5 0 166 157 111 628.5 0 111 158 93 628.5 0 93 159 108 628.5 0 108 160 63 628.5 0 63 161 58 628.5 0 58 162 84 628.5 0 84 163 61 628.5 0 61 164 113 628.5 0 113 165 45 628.5 0 45 166 49 628.5 0 49 167 62 628.5 0 62 168 253 628.5 0 253 169 112 628.5 0 112 170 127 628.5 0 127 171 83 628.5 0 83 172 307 628.5 0 307 173 319 628.5 0 319 174 358 628.5 0 358 175 336 628.5 0 336 176 90 628.5 0 90 177 111 628.5 0 111 178 141 628.5 0 141 179 98 628.5 0 98 180 64 628.5 0 64 181 60 628.5 0 60 182 112 628.5 0 112 183 138 628.5 0 138 184 216 628.5 0 216 185 136 628.5 0 136 186 200 628.5 0 200 187 311 628.5 0 311 188 3487 628.5 0 3487 189 1103 628.5 0 1103 190 3225 628.5 0 3225 191 118 628.5 0 118 192 53 628.5 0 53 193 74 628.5 0 74 194 81 628.5 0 81 195 118 628.5 0 118 196 211 628.5 0 211 197 211 628.5 0 211 198 247 628.5 0 247 199 370 628.5 0 370 200 635 628.5 0 635 201 6263 628.5 0 6263 202 2147 628.5 0 2147 203 2958 628.5 0 2958 204 855 628.5 0 855 205 134 628.5 0 134 206 115 628.5 0 115 207 52 628.5 0 52 208 46 628.5 0 46 209 31 628.5 0 31 210 60 628.5 0 60 211 40 628.5 0 40 212 34 628.5 0 34 213 49 628.5 0 49 214 87 628.5 0 87 215 141 628.5 0 141 216 94 628.5 0 94 217 59 628.5 0 59 218 25 628.5 0 25 219 36 628.5 0 36 220 56 628.5 0 56 221 52 628.5 0 52 222 54 628.5 0 54 223 60 628.5 0 60 224 93 628.5 0 93 225 126 628.5 0 126 226 130 628.5 0 130 227 159 628.5 0 159 228 293 628.5 0 293 229 228 628.5 0 228 230 274 628.5 0 274 231 133 628.5 0 133 232 62 628.5 0 62 233 54 628.5 0 54 234 73 628.5 0 73 235 60 628.5 0 60 236 72 628.5 0 72 237 45 628.5 0 45 238 47 628.5 0 47 239 98 628.5 0 98 240 79 628.5 0 79 241 171 628.5 0 171 242 52 628.5 0 52 243 105 628.5 0 105 244 100 628.5 0 100 245 118 628.5 0 118 246 78 628.5 0 78 247 81 628.5 0 81 248 97 628.5 0 97 249 149 628.5 0 149 250 275 628.5 0 275 251 102 628.5 0 102 252 162 628.5 0 162 253 101 628.5 0 101 254 79 628.5 0 79 255 40 628.5 0 40 256 50 628.5 0 50 257 60 628.5 0 60 258 95 628.5 0 95 259 103 628.5 0 103 260 164 628.5 0 164 261 136 628.5 0 136 262 125 628.5 0 125 263 129 628.5 0 129 264 78 628.5 0 78 265 85 628.5 0 85 266 94 628.5 0 94 267 94 628.5 0 94 268 105 628.5 0 105 269 817 628.5 0 817 270 419 628.5 0 419 271 748 628.5 0 748 272 127 628.5 0 127 273 76 628.5 0 76 274 127 628.5 0 127 275 228 628.5 0 228 276 299 628.5 0 299 277 145 628.5 0 145 278 157 628.5 0 157 279 90 628.5 0 90 280 269 628.5 0 269 281 284 628.5 0 284 282 552 628.5 0 552 283 288 628.5 0 288 284 374 628.5 0 374 285 141 628.5 0 141 286 118 628.5 0 118 287 144 628.5 0 144 288 223 628.5 0 223 289 166 628.5 0 166 290 128 628.5 0 128 291 572 628.5 0 572 292 701 628.5 0 701 293 974 628.5 0 974 294 840 628.5 0 840 295 577 628.5 0 577 296 719 628.5 0 719 297 231 628.5 0 231 298 64 628.5 0 64 299 135 628.5 0 135 300 147 628.5 0 147 301 207 628.5 0 207 Finished in 2.11 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 59,575 Reads with adapters: 5,242 (8.8%) Reads that were too short: 216 (0.4%) Reads written (passing filters): 5,026 (8.4%) Total basepairs processed: 10,152,640 bp Total written (filtered): 1,347,260 bp (13.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 1 2 16 46 0.0 3 0 35 7 4 17 4593 0.0 3 4058 428 81 26 18 39 0.0 3 2 16 15 6 19 139 0.0 3 7 4 17 111 20 20 0.0 3 1 0 0 19 21 5 0.0 3 0 0 0 5 22 19 0.0 3 0 0 0 19 24 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 3 0.0 3 0 0 0 3 36 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 0 4 42 3 0.0 3 0 0 0 3 43 10 0.0 3 0 0 0 10 44 2 0.0 3 0 0 0 2 45 58 0.0 3 0 0 0 58 46 6 0.0 3 0 0 0 6 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 56 7 0.0 3 0 0 0 7 57 10 0.0 3 0 0 0 10 59 1 0.0 3 0 0 0 1 60 5 0.0 3 0 0 0 5 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 5 0.0 3 0 0 0 5 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 1 1 69 1 0.0 3 0 0 0 1 70 12 0.0 3 0 0 2 10 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 79 8 0.0 3 0 0 0 8 80 3 0.0 3 0 0 0 3 82 4 0.0 3 0 0 1 3 83 2 0.0 3 1 0 0 1 85 1 0.0 3 0 0 0 1 86 6 0.0 3 0 0 0 6 87 22 0.0 3 0 0 0 22 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 4 0.0 3 0 0 0 4 91 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 120 3 0.0 3 0 0 0 3 121 3 0.0 3 0 0 0 3 122 4 0.0 3 0 0 0 4 123 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 10 0.0 3 0 0 1 9 132 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 0 4 134 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 1 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 3 0.0 3 0 0 0 3 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 1 203 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 78 0.0 3 0 0 5 73 218 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 5,026 Reads with adapters: 11 (0.2%) Reads written (passing filters): 5,026 (100.0%) Total basepairs processed: 1,347,260 bp Total written (filtered): 1,347,215 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 11 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.2% C: 36.4% G: 45.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 78.5 0 8 4 2 19.6 0 2 13 1 0.0 1 0 1