This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.08 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 61,879 (2.4%) Reads that were too short: 12,983 (0.5%) Reads written (passing filters): 48,896 (1.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 8,471,350 bp (1.1%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 61879 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 393 628.5 0 393 7 4258 628.5 0 4258 8 247 628.5 0 247 9 5191 628.5 0 5191 10 25 628.5 0 25 11 18 628.5 0 18 12 17 628.5 0 17 13 16 628.5 0 16 14 11 628.5 0 11 15 22 628.5 0 22 16 17 628.5 0 17 17 10 628.5 0 10 18 22 628.5 0 22 19 18 628.5 0 18 20 20 628.5 0 20 21 17 628.5 0 17 22 15 628.5 0 15 23 13 628.5 0 13 24 20 628.5 0 20 25 18 628.5 0 18 26 21 628.5 0 21 27 23 628.5 0 23 28 18 628.5 0 18 29 16 628.5 0 16 30 27 628.5 0 27 31 21 628.5 0 21 32 22 628.5 0 22 33 18 628.5 0 18 34 23 628.5 0 23 35 23 628.5 0 23 36 27 628.5 0 27 37 19 628.5 0 19 38 24 628.5 0 24 39 14 628.5 0 14 40 29 628.5 0 29 41 18 628.5 0 18 42 18 628.5 0 18 43 18 628.5 0 18 44 17 628.5 0 17 45 17 628.5 0 17 46 32 628.5 0 32 47 31 628.5 0 31 48 9 628.5 0 9 49 21 628.5 0 21 50 28 628.5 0 28 51 25 628.5 0 25 52 9 628.5 0 9 53 15 628.5 0 15 54 18 628.5 0 18 55 44 628.5 0 44 56 34 628.5 0 34 57 53 628.5 0 53 58 17 628.5 0 17 59 23 628.5 0 23 60 19 628.5 0 19 61 21 628.5 0 21 62 17 628.5 0 17 63 25 628.5 0 25 64 17 628.5 0 17 65 27 628.5 0 27 66 24 628.5 0 24 67 18 628.5 0 18 68 18 628.5 0 18 69 20 628.5 0 20 70 21 628.5 0 21 71 21 628.5 0 21 72 19 628.5 0 19 73 22 628.5 0 22 74 37 628.5 0 37 75 31 628.5 0 31 76 36 628.5 0 36 77 15 628.5 0 15 78 20 628.5 0 20 79 35 628.5 0 35 80 15 628.5 0 15 81 18 628.5 0 18 82 23 628.5 0 23 83 16 628.5 0 16 84 38 628.5 0 38 85 26 628.5 0 26 86 30 628.5 0 30 87 28 628.5 0 28 88 24 628.5 0 24 89 30 628.5 0 30 90 32 628.5 0 32 91 14 628.5 0 14 92 10 628.5 0 10 93 25 628.5 0 25 94 37 628.5 0 37 95 31 628.5 0 31 96 21 628.5 0 21 97 24 628.5 0 24 98 22 628.5 0 22 99 25 628.5 0 25 100 12 628.5 0 12 101 29 628.5 0 29 102 28 628.5 0 28 103 63 628.5 0 63 104 34 628.5 0 34 105 123 628.5 0 123 106 173 628.5 0 173 107 111 628.5 0 111 108 12 628.5 0 12 109 31 628.5 0 31 110 27 628.5 0 27 111 35 628.5 0 35 112 30 628.5 0 30 113 49 628.5 0 49 114 62 628.5 0 62 115 213 628.5 0 213 116 24 628.5 0 24 117 24 628.5 0 24 118 36 628.5 0 36 119 21 628.5 0 21 120 27 628.5 0 27 121 40 628.5 0 40 122 38 628.5 0 38 123 24 628.5 0 24 124 28 628.5 0 28 125 29 628.5 0 29 126 76 628.5 0 76 127 117 628.5 0 117 128 78 628.5 0 78 129 110 628.5 0 110 130 101 628.5 0 101 131 57 628.5 0 57 132 88 628.5 0 88 133 83 628.5 0 83 134 140 628.5 0 140 135 43 628.5 0 43 136 71 628.5 0 71 137 94 628.5 0 94 138 81 628.5 0 81 139 50 628.5 0 50 140 85 628.5 0 85 141 85 628.5 0 85 142 124 628.5 0 124 143 101 628.5 0 101 144 170 628.5 0 170 145 173 628.5 0 173 146 212 628.5 0 212 147 4675 628.5 0 4675 148 7970 628.5 0 7970 149 4846 628.5 0 4846 150 811 628.5 0 811 151 74 628.5 0 74 152 138 628.5 0 138 153 77 628.5 0 77 154 123 628.5 0 123 155 39 628.5 0 39 156 122 628.5 0 122 157 29 628.5 0 29 158 27 628.5 0 27 159 43 628.5 0 43 160 25 628.5 0 25 161 30 628.5 0 30 162 52 628.5 0 52 163 30 628.5 0 30 164 27 628.5 0 27 165 27 628.5 0 27 166 24 628.5 0 24 167 35 628.5 0 35 168 163 628.5 0 163 169 47 628.5 0 47 170 86 628.5 0 86 171 65 628.5 0 65 172 156 628.5 0 156 173 111 628.5 0 111 174 200 628.5 0 200 175 82 628.5 0 82 176 23 628.5 0 23 177 32 628.5 0 32 178 39 628.5 0 39 179 33 628.5 0 33 180 35 628.5 0 35 181 25 628.5 0 25 182 49 628.5 0 49 183 56 628.5 0 56 184 83 628.5 0 83 185 41 628.5 0 41 186 94 628.5 0 94 187 142 628.5 0 142 188 1336 628.5 0 1336 189 450 628.5 0 450 190 1589 628.5 0 1589 191 47 628.5 0 47 192 54 628.5 0 54 193 40 628.5 0 40 194 43 628.5 0 43 195 47 628.5 0 47 196 63 628.5 0 63 197 126 628.5 0 126 198 118 628.5 0 118 199 302 628.5 0 302 200 559 628.5 0 559 201 7782 628.5 0 7782 202 2249 628.5 0 2249 203 4396 628.5 0 4396 204 848 628.5 0 848 205 29 628.5 0 29 206 36 628.5 0 36 207 24 628.5 0 24 208 34 628.5 0 34 209 25 628.5 0 25 210 20 628.5 0 20 211 25 628.5 0 25 212 22 628.5 0 22 213 21 628.5 0 21 214 17 628.5 0 17 215 42 628.5 0 42 216 24 628.5 0 24 217 37 628.5 0 37 218 19 628.5 0 19 219 19 628.5 0 19 220 13 628.5 0 13 221 19 628.5 0 19 222 27 628.5 0 27 223 19 628.5 0 19 224 30 628.5 0 30 225 65 628.5 0 65 226 46 628.5 0 46 227 88 628.5 0 88 228 143 628.5 0 143 229 111 628.5 0 111 230 130 628.5 0 130 231 30 628.5 0 30 232 30 628.5 0 30 233 17 628.5 0 17 234 27 628.5 0 27 235 28 628.5 0 28 236 34 628.5 0 34 237 26 628.5 0 26 238 23 628.5 0 23 239 24 628.5 0 24 240 35 628.5 0 35 241 32 628.5 0 32 242 44 628.5 0 44 243 33 628.5 0 33 244 36 628.5 0 36 245 43 628.5 0 43 246 13 628.5 0 13 247 28 628.5 0 28 248 37 628.5 0 37 249 41 628.5 0 41 250 65 628.5 0 65 251 29 628.5 0 29 252 57 628.5 0 57 253 33 628.5 0 33 254 35 628.5 0 35 255 13 628.5 0 13 256 14 628.5 0 14 257 33 628.5 0 33 258 30 628.5 0 30 259 39 628.5 0 39 260 41 628.5 0 41 261 40 628.5 0 40 262 47 628.5 0 47 263 27 628.5 0 27 264 26 628.5 0 26 265 27 628.5 0 27 266 27 628.5 0 27 267 45 628.5 0 45 268 48 628.5 0 48 269 221 628.5 0 221 270 100 628.5 0 100 271 182 628.5 0 182 272 52 628.5 0 52 273 29 628.5 0 29 274 53 628.5 0 53 275 86 628.5 0 86 276 117 628.5 0 117 277 48 628.5 0 48 278 46 628.5 0 46 279 33 628.5 0 33 280 85 628.5 0 85 281 63 628.5 0 63 282 186 628.5 0 186 283 110 628.5 0 110 284 131 628.5 0 131 285 34 628.5 0 34 286 37 628.5 0 37 287 44 628.5 0 44 288 73 628.5 0 73 289 52 628.5 0 52 290 50 628.5 0 50 291 167 628.5 0 167 292 224 628.5 0 224 293 213 628.5 0 213 294 218 628.5 0 218 295 102 628.5 0 102 296 122 628.5 0 122 297 67 628.5 0 67 298 17 628.5 0 17 299 67 628.5 0 67 300 47 628.5 0 47 301 72 628.5 0 72 Finished in 1.97 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 48,896 Reads with adapters: 5,777 (11.8%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 5,769 (11.8%) Total basepairs processed: 8,471,350 bp Total written (filtered): 1,540,756 bp (18.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5777 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 85 0.0 3 0 85 20 5391 0.0 4 5308 81 2 21 12 0.0 4 4 8 22 1 0.0 4 1 23 3 0.0 4 1 0 0 2 35 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 1 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 1 89 2 0.0 4 0 0 0 0 2 126 3 0.0 4 0 0 0 0 3 127 270 0.0 4 0 0 0 0 270 130 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 5,769 Reads with adapters: 1,098 (19.0%) Reads written (passing filters): 5,769 (100.0%) Total basepairs processed: 1,540,756 bp Total written (filtered): 1,527,815 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1098 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 3.4% G: 94.4% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 47 90.1 0 47 4 7 22.5 0 7 5 1 5.6 0 1 9 668 0.0 0 23 645 10 309 0.0 1 15 294 11 51 0.0 1 0 51 12 1 0.0 1 0 1 13 1 0.0 1 0 1 110 1 0.0 1 1 223 2 0.0 1 2 236 2 0.0 1 2 256 7 0.0 1 7 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.