This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.18 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 384,596 (14.9%) Reads that were too short: 319,488 (12.4%) Reads written (passing filters): 65,108 (2.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 14,742,186 bp (1.9%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 384596 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 872 628.5 0 872 7 5721 628.5 0 5721 8 15775 628.5 0 15775 9 12091 628.5 0 12091 10 65 628.5 0 65 11 56 628.5 0 56 12 52 628.5 0 52 13 62 628.5 0 62 14 55 628.5 0 55 15 31 628.5 0 31 16 61 628.5 0 61 17 42 628.5 0 42 18 67 628.5 0 67 19 58 628.5 0 58 20 129 628.5 0 129 21 62 628.5 0 62 22 114 628.5 0 114 23 53 628.5 0 53 24 46 628.5 0 46 25 42 628.5 0 42 26 56 628.5 0 56 27 72 628.5 0 72 28 47 628.5 0 47 29 35 628.5 0 35 30 34 628.5 0 34 31 48 628.5 0 48 32 44 628.5 0 44 33 196 628.5 0 196 34 50 628.5 0 50 35 63 628.5 0 63 36 34 628.5 0 34 37 25 628.5 0 25 38 51 628.5 0 51 39 42 628.5 0 42 40 35 628.5 0 35 41 39 628.5 0 39 42 51 628.5 0 51 43 39 628.5 0 39 44 35 628.5 0 35 45 42 628.5 0 42 46 50 628.5 0 50 47 52 628.5 0 52 48 52 628.5 0 52 49 58 628.5 0 58 50 50 628.5 0 50 51 50 628.5 0 50 52 35 628.5 0 35 53 41 628.5 0 41 54 41 628.5 0 41 55 40 628.5 0 40 56 19 628.5 0 19 57 40 628.5 0 40 58 28 628.5 0 28 59 34 628.5 0 34 60 37 628.5 0 37 61 37 628.5 0 37 62 43 628.5 0 43 63 82 628.5 0 82 64 93 628.5 0 93 65 44 628.5 0 44 66 75 628.5 0 75 67 90 628.5 0 90 68 51 628.5 0 51 69 49 628.5 0 49 70 29 628.5 0 29 71 51 628.5 0 51 72 74 628.5 0 74 73 62 628.5 0 62 74 76 628.5 0 76 75 86 628.5 0 86 76 82 628.5 0 82 77 69 628.5 0 69 78 61 628.5 0 61 79 51 628.5 0 51 80 46 628.5 0 46 81 33 628.5 0 33 82 56 628.5 0 56 83 37 628.5 0 37 84 33 628.5 0 33 85 60 628.5 0 60 86 60 628.5 0 60 87 55 628.5 0 55 88 75 628.5 0 75 89 68 628.5 0 68 90 59 628.5 0 59 91 84 628.5 0 84 92 72 628.5 0 72 93 82 628.5 0 82 94 127 628.5 0 127 95 186 628.5 0 186 96 95 628.5 0 95 97 66 628.5 0 66 98 63 628.5 0 63 99 102 628.5 0 102 100 48 628.5 0 48 101 52 628.5 0 52 102 48 628.5 0 48 103 44 628.5 0 44 104 27 628.5 0 27 105 48 628.5 0 48 106 48 628.5 0 48 107 69 628.5 0 69 108 48 628.5 0 48 109 46 628.5 0 46 110 41 628.5 0 41 111 42 628.5 0 42 112 33 628.5 0 33 113 50 628.5 0 50 114 25 628.5 0 25 115 25 628.5 0 25 116 39 628.5 0 39 117 40 628.5 0 40 118 57 628.5 0 57 119 59 628.5 0 59 120 78 628.5 0 78 121 67 628.5 0 67 122 65 628.5 0 65 123 60 628.5 0 60 124 92 628.5 0 92 125 119 628.5 0 119 126 239 628.5 0 239 127 251 628.5 0 251 128 106 628.5 0 106 129 121 628.5 0 121 130 167 628.5 0 167 131 4092 628.5 0 4092 132 519 628.5 0 519 133 602 628.5 0 602 134 67 628.5 0 67 135 72 628.5 0 72 136 66 628.5 0 66 137 58 628.5 0 58 138 48 628.5 0 48 139 54 628.5 0 54 140 55 628.5 0 55 141 74 628.5 0 74 142 52 628.5 0 52 143 78 628.5 0 78 144 63 628.5 0 63 145 86 628.5 0 86 146 90 628.5 0 90 147 131 628.5 0 131 148 68 628.5 0 68 149 56 628.5 0 56 150 32 628.5 0 32 151 37 628.5 0 37 152 49 628.5 0 49 153 75 628.5 0 75 154 111 628.5 0 111 155 170 628.5 0 170 156 207 628.5 0 207 157 55 628.5 0 55 158 46 628.5 0 46 159 40 628.5 0 40 160 55 628.5 0 55 161 56 628.5 0 56 162 54 628.5 0 54 163 37 628.5 0 37 164 46 628.5 0 46 165 63 628.5 0 63 166 66 628.5 0 66 167 57 628.5 0 57 168 65 628.5 0 65 169 76 628.5 0 76 170 59 628.5 0 59 171 56 628.5 0 56 172 55 628.5 0 55 173 131 628.5 0 131 174 216 628.5 0 216 175 269 628.5 0 269 176 167 628.5 0 167 177 82 628.5 0 82 178 76 628.5 0 76 179 339 628.5 0 339 180 470 628.5 0 470 181 510 628.5 0 510 182 153 628.5 0 153 183 111 628.5 0 111 184 84 628.5 0 84 185 290 628.5 0 290 186 182 628.5 0 182 187 406 628.5 0 406 188 125 628.5 0 125 189 136 628.5 0 136 190 154 628.5 0 154 191 238 628.5 0 238 192 497 628.5 0 497 193 3142 628.5 0 3142 194 1703 628.5 0 1703 195 3108 628.5 0 3108 196 231 628.5 0 231 197 137 628.5 0 137 198 131 628.5 0 131 199 483 628.5 0 483 200 700 628.5 0 700 201 902 628.5 0 902 202 717 628.5 0 717 203 120 628.5 0 120 204 80 628.5 0 80 205 172 628.5 0 172 206 191 628.5 0 191 207 209 628.5 0 209 208 144 628.5 0 144 209 76 628.5 0 76 210 102 628.5 0 102 211 146 628.5 0 146 212 154 628.5 0 154 213 289 628.5 0 289 214 167 628.5 0 167 215 128 628.5 0 128 216 107 628.5 0 107 217 133 628.5 0 133 218 153 628.5 0 153 219 232 628.5 0 232 220 285 628.5 0 285 221 382 628.5 0 382 222 450 628.5 0 450 223 519 628.5 0 519 224 824 628.5 0 824 225 281 628.5 0 281 226 338 628.5 0 338 227 558 628.5 0 558 228 323 628.5 0 323 229 390 628.5 0 390 230 683 628.5 0 683 231 862 628.5 0 862 232 4424 628.5 0 4424 233 5193 628.5 0 5193 234 3787 628.5 0 3787 235 1417 628.5 0 1417 236 2091 628.5 0 2091 237 25805 628.5 0 25805 238 33713 628.5 0 33713 239 47347 628.5 0 47347 240 1456 628.5 0 1456 241 767 628.5 0 767 242 551 628.5 0 551 243 787 628.5 0 787 244 684 628.5 0 684 245 494 628.5 0 494 246 396 628.5 0 396 247 517 628.5 0 517 248 456 628.5 0 456 249 755 628.5 0 755 250 1213 628.5 0 1213 251 1416 628.5 0 1416 252 1825 628.5 0 1825 253 1985 628.5 0 1985 254 1915 628.5 0 1915 255 1632 628.5 0 1632 256 2290 628.5 0 2290 257 41925 628.5 0 41925 258 60171 628.5 0 60171 259 43857 628.5 0 43857 260 14833 628.5 0 14833 261 209 628.5 0 209 262 133 628.5 0 133 263 135 628.5 0 135 264 56 628.5 0 56 265 41 628.5 0 41 266 73 628.5 0 73 267 100 628.5 0 100 268 193 628.5 0 193 269 164 628.5 0 164 270 155 628.5 0 155 271 102 628.5 0 102 272 99 628.5 0 99 273 117 628.5 0 117 274 72 628.5 0 72 275 85 628.5 0 85 276 75 628.5 0 75 277 51 628.5 0 51 278 76 628.5 0 76 279 82 628.5 0 82 280 90 628.5 0 90 281 99 628.5 0 99 282 112 628.5 0 112 283 122 628.5 0 122 284 95 628.5 0 95 285 161 628.5 0 161 286 195 628.5 0 195 287 341 628.5 0 341 288 168 628.5 0 168 289 267 628.5 0 267 290 407 628.5 0 407 291 606 628.5 0 606 292 346 628.5 0 346 293 452 628.5 0 452 294 201 628.5 0 201 295 128 628.5 0 128 296 140 628.5 0 140 297 109 628.5 0 109 298 72 628.5 0 72 299 77 628.5 0 77 300 173 628.5 0 173 301 192 628.5 0 192 Finished in 2.87 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 65,108 Reads with adapters: 21,216 (32.6%) Reads that were too short: 164 (0.3%) Reads written (passing filters): 21,052 (32.3%) Total basepairs processed: 14,742,186 bp Total written (filtered): 5,789,081 bp (39.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21216 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 14 0.0 3 0 0 8 6 16 169 0.0 3 0 127 30 12 17 15069 0.0 3 13649 1133 239 48 18 129 0.0 3 5 88 15 21 19 5472 0.0 3 4832 438 146 56 20 69 0.0 3 7 14 3 45 21 10 0.0 3 0 0 6 4 22 13 0.0 3 0 0 0 13 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 1 26 1 0.0 3 1 30 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 38 3 0.0 3 0 0 1 2 42 2 0.0 3 0 0 1 1 43 4 0.0 3 0 0 0 4 44 2 0.0 3 0 0 0 2 45 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 47 7 0.0 3 0 0 1 6 48 4 0.0 3 0 0 0 4 49 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 52 4 0.0 3 0 0 0 4 53 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 60 38 0.0 3 0 0 0 38 62 3 0.0 3 0 0 0 3 63 8 0.0 3 0 1 0 7 64 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 0 4 74 5 0.0 3 0 0 0 5 76 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 2 0.0 3 0 0 0 2 84 8 0.0 3 0 0 0 8 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 5 0.0 3 0 0 0 5 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 5 0.0 3 0 0 0 5 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 5 0.0 3 0 0 0 5 113 4 0.0 3 0 0 0 4 117 4 0.0 3 0 0 0 4 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 1 2 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 3 0.0 3 0 0 0 3 162 13 0.0 3 0 0 0 13 163 2 0.0 3 0 0 1 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 1 169 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 5 0.0 3 0 0 0 5 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.74 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 21,052 Reads with adapters: 209 (1.0%) Reads written (passing filters): 21,052 (100.0%) Total basepairs processed: 5,789,081 bp Total written (filtered): 5,788,426 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 209 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.5% C: 13.4% G: 27.8% T: 25.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 183 328.9 0 183 4 24 82.2 0 24 5 2 20.6 0 2