This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.77 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 458,694 (17.8%) Reads that were too short: 388,854 (15.1%) Reads written (passing filters): 69,840 (2.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 15,955,053 bp (2.1%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 458694 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 896 628.5 0 896 7 5330 628.5 0 5330 8 15475 628.5 0 15475 9 14794 628.5 0 14794 10 73 628.5 0 73 11 82 628.5 0 82 12 61 628.5 0 61 13 68 628.5 0 68 14 73 628.5 0 73 15 48 628.5 0 48 16 71 628.5 0 71 17 93 628.5 0 93 18 69 628.5 0 69 19 79 628.5 0 79 20 92 628.5 0 92 21 67 628.5 0 67 22 72 628.5 0 72 23 60 628.5 0 60 24 77 628.5 0 77 25 59 628.5 0 59 26 66 628.5 0 66 27 64 628.5 0 64 28 51 628.5 0 51 29 71 628.5 0 71 30 50 628.5 0 50 31 53 628.5 0 53 32 38 628.5 0 38 33 57 628.5 0 57 34 58 628.5 0 58 35 91 628.5 0 91 36 76 628.5 0 76 37 62 628.5 0 62 38 68 628.5 0 68 39 64 628.5 0 64 40 48 628.5 0 48 41 58 628.5 0 58 42 60 628.5 0 60 43 54 628.5 0 54 44 45 628.5 0 45 45 46 628.5 0 46 46 62 628.5 0 62 47 78 628.5 0 78 48 91 628.5 0 91 49 50 628.5 0 50 50 55 628.5 0 55 51 80 628.5 0 80 52 55 628.5 0 55 53 66 628.5 0 66 54 62 628.5 0 62 55 56 628.5 0 56 56 45 628.5 0 45 57 78 628.5 0 78 58 60 628.5 0 60 59 51 628.5 0 51 60 50 628.5 0 50 61 65 628.5 0 65 62 55 628.5 0 55 63 128 628.5 0 128 64 117 628.5 0 117 65 52 628.5 0 52 66 97 628.5 0 97 67 116 628.5 0 116 68 68 628.5 0 68 69 64 628.5 0 64 70 58 628.5 0 58 71 93 628.5 0 93 72 84 628.5 0 84 73 72 628.5 0 72 74 72 628.5 0 72 75 71 628.5 0 71 76 79 628.5 0 79 77 98 628.5 0 98 78 65 628.5 0 65 79 75 628.5 0 75 80 53 628.5 0 53 81 61 628.5 0 61 82 61 628.5 0 61 83 58 628.5 0 58 84 55 628.5 0 55 85 90 628.5 0 90 86 65 628.5 0 65 87 91 628.5 0 91 88 81 628.5 0 81 89 90 628.5 0 90 90 92 628.5 0 92 91 82 628.5 0 82 92 84 628.5 0 84 93 82 628.5 0 82 94 73 628.5 0 73 95 73 628.5 0 73 96 58 628.5 0 58 97 84 628.5 0 84 98 69 628.5 0 69 99 90 628.5 0 90 100 82 628.5 0 82 101 71 628.5 0 71 102 74 628.5 0 74 103 63 628.5 0 63 104 45 628.5 0 45 105 67 628.5 0 67 106 85 628.5 0 85 107 75 628.5 0 75 108 71 628.5 0 71 109 66 628.5 0 66 110 74 628.5 0 74 111 61 628.5 0 61 112 70 628.5 0 70 113 75 628.5 0 75 114 76 628.5 0 76 115 69 628.5 0 69 116 80 628.5 0 80 117 84 628.5 0 84 118 78 628.5 0 78 119 69 628.5 0 69 120 92 628.5 0 92 121 82 628.5 0 82 122 83 628.5 0 83 123 94 628.5 0 94 124 105 628.5 0 105 125 124 628.5 0 124 126 158 628.5 0 158 127 146 628.5 0 146 128 87 628.5 0 87 129 145 628.5 0 145 130 181 628.5 0 181 131 5390 628.5 0 5390 132 518 628.5 0 518 133 418 628.5 0 418 134 102 628.5 0 102 135 113 628.5 0 113 136 88 628.5 0 88 137 112 628.5 0 112 138 79 628.5 0 79 139 95 628.5 0 95 140 91 628.5 0 91 141 127 628.5 0 127 142 108 628.5 0 108 143 116 628.5 0 116 144 106 628.5 0 106 145 93 628.5 0 93 146 119 628.5 0 119 147 141 628.5 0 141 148 115 628.5 0 115 149 105 628.5 0 105 150 105 628.5 0 105 151 129 628.5 0 129 152 115 628.5 0 115 153 134 628.5 0 134 154 149 628.5 0 149 155 142 628.5 0 142 156 160 628.5 0 160 157 97 628.5 0 97 158 113 628.5 0 113 159 114 628.5 0 114 160 126 628.5 0 126 161 104 628.5 0 104 162 123 628.5 0 123 163 124 628.5 0 124 164 125 628.5 0 125 165 152 628.5 0 152 166 127 628.5 0 127 167 152 628.5 0 152 168 141 628.5 0 141 169 149 628.5 0 149 170 110 628.5 0 110 171 139 628.5 0 139 172 148 628.5 0 148 173 199 628.5 0 199 174 228 628.5 0 228 175 253 628.5 0 253 176 205 628.5 0 205 177 144 628.5 0 144 178 133 628.5 0 133 179 172 628.5 0 172 180 290 628.5 0 290 181 309 628.5 0 309 182 163 628.5 0 163 183 159 628.5 0 159 184 158 628.5 0 158 185 381 628.5 0 381 186 272 628.5 0 272 187 443 628.5 0 443 188 211 628.5 0 211 189 194 628.5 0 194 190 242 628.5 0 242 191 273 628.5 0 273 192 351 628.5 0 351 193 2084 628.5 0 2084 194 1552 628.5 0 1552 195 3042 628.5 0 3042 196 230 628.5 0 230 197 222 628.5 0 222 198 191 628.5 0 191 199 315 628.5 0 315 200 449 628.5 0 449 201 450 628.5 0 450 202 356 628.5 0 356 203 155 628.5 0 155 204 135 628.5 0 135 205 184 628.5 0 184 206 205 628.5 0 205 207 226 628.5 0 226 208 178 628.5 0 178 209 154 628.5 0 154 210 192 628.5 0 192 211 225 628.5 0 225 212 209 628.5 0 209 213 300 628.5 0 300 214 234 628.5 0 234 215 242 628.5 0 242 216 199 628.5 0 199 217 245 628.5 0 245 218 242 628.5 0 242 219 337 628.5 0 337 220 325 628.5 0 325 221 354 628.5 0 354 222 463 628.5 0 463 223 594 628.5 0 594 224 923 628.5 0 923 225 411 628.5 0 411 226 450 628.5 0 450 227 803 628.5 0 803 228 466 628.5 0 466 229 474 628.5 0 474 230 713 628.5 0 713 231 1039 628.5 0 1039 232 5689 628.5 0 5689 233 7137 628.5 0 7137 234 4854 628.5 0 4854 235 1445 628.5 0 1445 236 2038 628.5 0 2038 237 30070 628.5 0 30070 238 43369 628.5 0 43369 239 52756 628.5 0 52756 240 1363 628.5 0 1363 241 761 628.5 0 761 242 747 628.5 0 747 243 987 628.5 0 987 244 942 628.5 0 942 245 819 628.5 0 819 246 545 628.5 0 545 247 683 628.5 0 683 248 740 628.5 0 740 249 1103 628.5 0 1103 250 1739 628.5 0 1739 251 2023 628.5 0 2023 252 2262 628.5 0 2262 253 2538 628.5 0 2538 254 2274 628.5 0 2274 255 1775 628.5 0 1775 256 2314 628.5 0 2314 257 44845 628.5 0 44845 258 72462 628.5 0 72462 259 55750 628.5 0 55750 260 25065 628.5 0 25065 261 234 628.5 0 234 262 151 628.5 0 151 263 168 628.5 0 168 264 147 628.5 0 147 265 145 628.5 0 145 266 152 628.5 0 152 267 168 628.5 0 168 268 246 628.5 0 246 269 301 628.5 0 301 270 230 628.5 0 230 271 152 628.5 0 152 272 158 628.5 0 158 273 160 628.5 0 160 274 139 628.5 0 139 275 133 628.5 0 133 276 181 628.5 0 181 277 154 628.5 0 154 278 136 628.5 0 136 279 179 628.5 0 179 280 203 628.5 0 203 281 176 628.5 0 176 282 166 628.5 0 166 283 166 628.5 0 166 284 179 628.5 0 179 285 157 628.5 0 157 286 162 628.5 0 162 287 170 628.5 0 170 288 164 628.5 0 164 289 204 628.5 0 204 290 311 628.5 0 311 291 732 628.5 0 732 292 466 628.5 0 466 293 605 628.5 0 605 294 297 628.5 0 297 295 184 628.5 0 184 296 172 628.5 0 172 297 172 628.5 0 172 298 160 628.5 0 160 299 171 628.5 0 171 300 199 628.5 0 199 301 176 628.5 0 176 Finished in 3.29 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 69,840 Reads with adapters: 16,292 (23.3%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 16,283 (23.3%) Total basepairs processed: 15,955,053 bp Total written (filtered): 4,393,736 bp (27.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16292 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 1 6 18 15 0.0 3 0 0 14 0 1 19 325 0.0 3 0 322 2 1 20 15538 0.0 4 15289 235 13 1 21 28 0.0 4 7 20 0 1 22 11 0.0 4 7 0 3 0 1 23 14 0.0 4 1 0 0 12 1 60 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 237 0.0 4 0 0 0 0 237 128 1 0.0 4 0 0 0 0 1 129 97 0.0 4 0 0 0 0 97 130 1 0.0 4 0 0 0 0 1 137 4 0.0 4 0 0 0 0 4 208 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 252 1 0.0 4 0 0 1 262 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 16,283 Reads with adapters: 2,184 (13.4%) Reads written (passing filters): 16,283 (100.0%) Total basepairs processed: 4,393,736 bp Total written (filtered): 4,371,715 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2184 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 3.9% G: 93.6% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 254.4 0 7 4 8 63.6 0 8 6 17 4.0 0 17 7 1 1.0 0 1 9 837 0.1 0 23 814 10 1049 0.0 1 49 1000 11 255 0.0 1 1 254 12 3 0.0 1 0 3 13 1 0.0 1 0 1 150 1 0.0 1 0 1 156 1 0.0 1 1 169 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.