This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.32 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 82,710 (3.2%) Reads that were too short: 7,682 (0.3%) Reads written (passing filters): 75,028 (2.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 18,889,558 bp (2.4%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 82710 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 790 628.5 0 790 7 25595 628.5 0 25595 8 560 628.5 0 560 9 22372 628.5 0 22372 10 37 628.5 0 37 11 28 628.5 0 28 12 25 628.5 0 25 13 25 628.5 0 25 14 29 628.5 0 29 15 29 628.5 0 29 16 37 628.5 0 37 17 32 628.5 0 32 18 22 628.5 0 22 19 16 628.5 0 16 20 25 628.5 0 25 21 21 628.5 0 21 22 34 628.5 0 34 23 34 628.5 0 34 24 18 628.5 0 18 25 42 628.5 0 42 26 69 628.5 0 69 27 50 628.5 0 50 28 126 628.5 0 126 29 71 628.5 0 71 30 87 628.5 0 87 31 58 628.5 0 58 32 20 628.5 0 20 33 25 628.5 0 25 34 40 628.5 0 40 35 39 628.5 0 39 36 24 628.5 0 24 37 23 628.5 0 23 38 27 628.5 0 27 39 34 628.5 0 34 40 29 628.5 0 29 41 10 628.5 0 10 42 32 628.5 0 32 43 32 628.5 0 32 44 31 628.5 0 31 45 27 628.5 0 27 46 22 628.5 0 22 47 18 628.5 0 18 48 13 628.5 0 13 49 40 628.5 0 40 50 44 628.5 0 44 51 45 628.5 0 45 52 39 628.5 0 39 53 49 628.5 0 49 54 32 628.5 0 32 55 37 628.5 0 37 56 33 628.5 0 33 57 34 628.5 0 34 58 42 628.5 0 42 59 33 628.5 0 33 60 41 628.5 0 41 61 42 628.5 0 42 62 35 628.5 0 35 63 37 628.5 0 37 64 41 628.5 0 41 65 28 628.5 0 28 66 38 628.5 0 38 67 56 628.5 0 56 68 52 628.5 0 52 69 434 628.5 0 434 70 210 628.5 0 210 71 427 628.5 0 427 72 34 628.5 0 34 73 37 628.5 0 37 74 33 628.5 0 33 75 61 628.5 0 61 76 40 628.5 0 40 77 55 628.5 0 55 78 48 628.5 0 48 79 75 628.5 0 75 80 35 628.5 0 35 81 41 628.5 0 41 82 39 628.5 0 39 83 46 628.5 0 46 84 35 628.5 0 35 85 34 628.5 0 34 86 50 628.5 0 50 87 51 628.5 0 51 88 43 628.5 0 43 89 34 628.5 0 34 90 47 628.5 0 47 91 44 628.5 0 44 92 48 628.5 0 48 93 42 628.5 0 42 94 38 628.5 0 38 95 39 628.5 0 39 96 81 628.5 0 81 97 46 628.5 0 46 98 58 628.5 0 58 99 48 628.5 0 48 100 48 628.5 0 48 101 49 628.5 0 49 102 39 628.5 0 39 103 26 628.5 0 26 104 44 628.5 0 44 105 47 628.5 0 47 106 37 628.5 0 37 107 51 628.5 0 51 108 48 628.5 0 48 109 34 628.5 0 34 110 43 628.5 0 43 111 53 628.5 0 53 112 56 628.5 0 56 113 50 628.5 0 50 114 220 628.5 0 220 115 356 628.5 0 356 116 77 628.5 0 77 117 40 628.5 0 40 118 42 628.5 0 42 119 36 628.5 0 36 120 25 628.5 0 25 121 35 628.5 0 35 122 39 628.5 0 39 123 53 628.5 0 53 124 68 628.5 0 68 125 75 628.5 0 75 126 106 628.5 0 106 127 109 628.5 0 109 128 132 628.5 0 132 129 88 628.5 0 88 130 65 628.5 0 65 131 69 628.5 0 69 132 76 628.5 0 76 133 281 628.5 0 281 134 124 628.5 0 124 135 88 628.5 0 88 136 123 628.5 0 123 137 138 628.5 0 138 138 732 628.5 0 732 139 1999 628.5 0 1999 140 4015 628.5 0 4015 141 2865 628.5 0 2865 142 365 628.5 0 365 143 368 628.5 0 368 144 525 628.5 0 525 145 498 628.5 0 498 146 66 628.5 0 66 147 186 628.5 0 186 148 300 628.5 0 300 149 1190 628.5 0 1190 150 1945 628.5 0 1945 151 1087 628.5 0 1087 152 154 628.5 0 154 153 96 628.5 0 96 154 46 628.5 0 46 155 67 628.5 0 67 156 31 628.5 0 31 157 39 628.5 0 39 158 28 628.5 0 28 159 34 628.5 0 34 160 27 628.5 0 27 161 24 628.5 0 24 162 23 628.5 0 23 163 18 628.5 0 18 164 24 628.5 0 24 165 31 628.5 0 31 166 25 628.5 0 25 167 23 628.5 0 23 168 31 628.5 0 31 169 28 628.5 0 28 170 37 628.5 0 37 171 13 628.5 0 13 172 36 628.5 0 36 173 38 628.5 0 38 174 25 628.5 0 25 175 30 628.5 0 30 176 33 628.5 0 33 177 29 628.5 0 29 178 23 628.5 0 23 179 37 628.5 0 37 180 27 628.5 0 27 181 30 628.5 0 30 182 26 628.5 0 26 183 39 628.5 0 39 184 34 628.5 0 34 185 52 628.5 0 52 186 36 628.5 0 36 187 38 628.5 0 38 188 46 628.5 0 46 189 63 628.5 0 63 190 86 628.5 0 86 191 123 628.5 0 123 192 40 628.5 0 40 193 23 628.5 0 23 194 50 628.5 0 50 195 24 628.5 0 24 196 22 628.5 0 22 197 29 628.5 0 29 198 23 628.5 0 23 199 39 628.5 0 39 200 24 628.5 0 24 201 44 628.5 0 44 202 27 628.5 0 27 203 33 628.5 0 33 204 26 628.5 0 26 205 23 628.5 0 23 206 28 628.5 0 28 207 34 628.5 0 34 208 24 628.5 0 24 209 159 628.5 0 159 210 62 628.5 0 62 211 38 628.5 0 38 212 40 628.5 0 40 213 33 628.5 0 33 214 38 628.5 0 38 215 33 628.5 0 33 216 18 628.5 0 18 217 32 628.5 0 32 218 25 628.5 0 25 219 24 628.5 0 24 220 25 628.5 0 25 221 21 628.5 0 21 222 31 628.5 0 31 223 28 628.5 0 28 224 26 628.5 0 26 225 24 628.5 0 24 226 22 628.5 0 22 227 21 628.5 0 21 228 18 628.5 0 18 229 32 628.5 0 32 230 26 628.5 0 26 231 30 628.5 0 30 232 29 628.5 0 29 233 61 628.5 0 61 234 93 628.5 0 93 235 91 628.5 0 91 236 54 628.5 0 54 237 27 628.5 0 27 238 54 628.5 0 54 239 30 628.5 0 30 240 28 628.5 0 28 241 44 628.5 0 44 242 45 628.5 0 45 243 43 628.5 0 43 244 49 628.5 0 49 245 44 628.5 0 44 246 40 628.5 0 40 247 45 628.5 0 45 248 71 628.5 0 71 249 83 628.5 0 83 250 189 628.5 0 189 251 35 628.5 0 35 252 43 628.5 0 43 253 317 628.5 0 317 254 484 628.5 0 484 255 734 628.5 0 734 256 93 628.5 0 93 257 27 628.5 0 27 258 50 628.5 0 50 259 26 628.5 0 26 260 41 628.5 0 41 261 36 628.5 0 36 262 45 628.5 0 45 263 26 628.5 0 26 264 43 628.5 0 43 265 28 628.5 0 28 266 33 628.5 0 33 267 39 628.5 0 39 268 54 628.5 0 54 269 75 628.5 0 75 270 95 628.5 0 95 271 49 628.5 0 49 272 78 628.5 0 78 273 147 628.5 0 147 274 201 628.5 0 201 275 115 628.5 0 115 276 68 628.5 0 68 277 40 628.5 0 40 278 75 628.5 0 75 279 69 628.5 0 69 280 43 628.5 0 43 281 60 628.5 0 60 282 72 628.5 0 72 283 107 628.5 0 107 284 29 628.5 0 29 285 82 628.5 0 82 286 69 628.5 0 69 287 76 628.5 0 76 288 94 628.5 0 94 289 100 628.5 0 100 290 54 628.5 0 54 291 70 628.5 0 70 292 85 628.5 0 85 293 69 628.5 0 69 294 81 628.5 0 81 295 191 628.5 0 191 296 143 628.5 0 143 297 118 628.5 0 118 298 108 628.5 0 108 299 140 628.5 0 140 300 166 628.5 0 166 301 438 628.5 0 438 Finished in 3.74 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 75,028 Reads with adapters: 26,262 (35.0%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 26,247 (35.0%) Total basepairs processed: 18,889,558 bp Total written (filtered): 7,170,006 bp (38.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26262 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 10 19 359 0.0 3 0 350 8 0 1 20 25601 0.0 4 25181 407 12 1 21 47 0.0 4 5 40 1 1 22 7 0.0 4 2 0 5 23 14 0.0 4 1 0 0 12 1 24 1 0.0 4 0 0 1 28 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 65 1 0.0 4 1 66 2 0.0 4 2 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 9 0.0 4 0 0 0 0 9 113 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 187 0.0 4 0 0 0 0 187 128 1 0.0 4 1 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 138 1 0.0 4 1 196 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 229 3 0.0 4 0 0 0 0 3 233 1 0.0 4 1 234 1 0.0 4 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.91 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 26,247 Reads with adapters: 736 (2.8%) Reads written (passing filters): 26,247 (100.0%) Total basepairs processed: 7,170,006 bp Total written (filtered): 7,161,210 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 736 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.5% C: 5.8% G: 84.5% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 64 410.1 0 64 4 4 102.5 0 4 5 3 25.6 0 3 6 1 6.4 0 1 9 6 0.1 0 2 4 10 9 0.0 1 1 8 11 601 0.0 1 0 601 12 39 0.0 1 0 39 122 1 0.0 1 1 152 7 0.0 1 0 7 158 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.