This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.42 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 50,417 (2.0%) Reads that were too short: 20,542 (0.8%) Reads written (passing filters): 29,875 (1.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 7,058,209 bp (0.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 50417 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 267 628.5 0 267 7 221 628.5 0 221 8 6395 628.5 0 6395 9 5485 628.5 0 5485 10 32 628.5 0 32 11 2 628.5 0 2 12 2 628.5 0 2 13 6 628.5 0 6 14 6 628.5 0 6 15 6 628.5 0 6 16 11 628.5 0 11 17 8 628.5 0 8 18 13 628.5 0 13 19 6 628.5 0 6 20 5 628.5 0 5 21 5 628.5 0 5 22 7 628.5 0 7 23 7 628.5 0 7 24 5 628.5 0 5 25 4 628.5 0 4 26 21 628.5 0 21 27 48 628.5 0 48 28 47 628.5 0 47 29 4 628.5 0 4 30 14 628.5 0 14 31 6 628.5 0 6 32 4 628.5 0 4 33 11 628.5 0 11 34 12 628.5 0 12 35 2 628.5 0 2 36 3 628.5 0 3 37 6 628.5 0 6 38 2 628.5 0 2 39 7 628.5 0 7 40 12 628.5 0 12 41 11 628.5 0 11 42 27 628.5 0 27 43 26 628.5 0 26 44 6 628.5 0 6 45 14 628.5 0 14 46 6 628.5 0 6 47 23 628.5 0 23 48 7 628.5 0 7 49 10 628.5 0 10 50 6 628.5 0 6 51 13 628.5 0 13 52 21 628.5 0 21 53 32 628.5 0 32 54 23 628.5 0 23 55 14 628.5 0 14 56 17 628.5 0 17 57 33 628.5 0 33 58 101 628.5 0 101 59 209 628.5 0 209 60 1953 628.5 0 1953 61 996 628.5 0 996 62 1947 628.5 0 1947 63 29 628.5 0 29 64 26 628.5 0 26 65 35 628.5 0 35 66 201 628.5 0 201 67 66 628.5 0 66 68 78 628.5 0 78 69 4 628.5 0 4 70 20 628.5 0 20 71 16 628.5 0 16 72 19 628.5 0 19 73 15 628.5 0 15 74 10 628.5 0 10 75 20 628.5 0 20 76 39 628.5 0 39 77 452 628.5 0 452 78 149 628.5 0 149 79 380 628.5 0 380 80 19 628.5 0 19 81 30 628.5 0 30 82 44 628.5 0 44 83 17 628.5 0 17 84 17 628.5 0 17 85 21 628.5 0 21 86 15 628.5 0 15 87 3 628.5 0 3 88 28 628.5 0 28 89 17 628.5 0 17 90 11 628.5 0 11 91 13 628.5 0 13 92 15 628.5 0 15 93 14 628.5 0 14 94 23 628.5 0 23 95 24 628.5 0 24 96 7 628.5 0 7 97 26 628.5 0 26 98 27 628.5 0 27 99 27 628.5 0 27 100 21 628.5 0 21 101 23 628.5 0 23 102 12 628.5 0 12 103 11 628.5 0 11 104 22 628.5 0 22 105 16 628.5 0 16 106 17 628.5 0 17 107 27 628.5 0 27 108 51 628.5 0 51 109 36 628.5 0 36 110 35 628.5 0 35 111 204 628.5 0 204 112 286 628.5 0 286 113 461 628.5 0 461 114 1039 628.5 0 1039 115 1438 628.5 0 1438 116 1564 628.5 0 1564 117 56 628.5 0 56 118 26 628.5 0 26 119 28 628.5 0 28 120 17 628.5 0 17 121 21 628.5 0 21 122 22 628.5 0 22 123 16 628.5 0 16 124 13 628.5 0 13 125 48 628.5 0 48 126 28 628.5 0 28 127 24 628.5 0 24 128 15 628.5 0 15 129 30 628.5 0 30 130 45 628.5 0 45 131 53 628.5 0 53 132 71 628.5 0 71 133 144 628.5 0 144 134 7 628.5 0 7 135 24 628.5 0 24 136 17 628.5 0 17 137 28 628.5 0 28 138 30 628.5 0 30 139 38 628.5 0 38 140 36 628.5 0 36 141 19 628.5 0 19 142 21 628.5 0 21 143 56 628.5 0 56 144 67 628.5 0 67 145 103 628.5 0 103 146 107 628.5 0 107 147 25 628.5 0 25 148 40 628.5 0 40 149 27 628.5 0 27 150 31 628.5 0 31 151 18 628.5 0 18 152 28 628.5 0 28 153 23 628.5 0 23 154 28 628.5 0 28 155 14 628.5 0 14 156 21 628.5 0 21 157 14 628.5 0 14 158 14 628.5 0 14 159 25 628.5 0 25 160 41 628.5 0 41 161 51 628.5 0 51 162 28 628.5 0 28 163 45 628.5 0 45 164 97 628.5 0 97 165 332 628.5 0 332 166 232 628.5 0 232 167 95 628.5 0 95 168 26 628.5 0 26 169 35 628.5 0 35 170 33 628.5 0 33 171 35 628.5 0 35 172 28 628.5 0 28 173 22 628.5 0 22 174 11 628.5 0 11 175 14 628.5 0 14 176 20 628.5 0 20 177 17 628.5 0 17 178 21 628.5 0 21 179 66 628.5 0 66 180 72 628.5 0 72 181 239 628.5 0 239 182 32 628.5 0 32 183 30 628.5 0 30 184 89 628.5 0 89 185 121 628.5 0 121 186 96 628.5 0 96 187 59 628.5 0 59 188 37 628.5 0 37 189 38 628.5 0 38 190 155 628.5 0 155 191 135 628.5 0 135 192 109 628.5 0 109 193 59 628.5 0 59 194 59 628.5 0 59 195 45 628.5 0 45 196 63 628.5 0 63 197 97 628.5 0 97 198 62 628.5 0 62 199 38 628.5 0 38 200 33 628.5 0 33 201 33 628.5 0 33 202 46 628.5 0 46 203 322 628.5 0 322 204 83 628.5 0 83 205 180 628.5 0 180 206 48 628.5 0 48 207 86 628.5 0 86 208 142 628.5 0 142 209 176 628.5 0 176 210 410 628.5 0 410 211 45 628.5 0 45 212 92 628.5 0 92 213 33 628.5 0 33 214 103 628.5 0 103 215 68 628.5 0 68 216 128 628.5 0 128 217 210 628.5 0 210 218 152 628.5 0 152 219 78 628.5 0 78 220 31 628.5 0 31 221 26 628.5 0 26 222 32 628.5 0 32 223 43 628.5 0 43 224 182 628.5 0 182 225 113 628.5 0 113 226 181 628.5 0 181 227 105 628.5 0 105 228 55 628.5 0 55 229 72 628.5 0 72 230 82 628.5 0 82 231 80 628.5 0 80 232 119 628.5 0 119 233 100 628.5 0 100 234 107 628.5 0 107 235 137 628.5 0 137 236 562 628.5 0 562 237 451 628.5 0 451 238 514 628.5 0 514 239 289 628.5 0 289 240 58 628.5 0 58 241 55 628.5 0 55 242 85 628.5 0 85 243 117 628.5 0 117 244 198 628.5 0 198 245 1830 628.5 0 1830 246 2759 628.5 0 2759 247 2346 628.5 0 2346 248 69 628.5 0 69 249 40 628.5 0 40 250 50 628.5 0 50 251 92 628.5 0 92 252 61 628.5 0 61 253 72 628.5 0 72 254 132 628.5 0 132 255 105 628.5 0 105 256 54 628.5 0 54 257 54 628.5 0 54 258 61 628.5 0 61 259 96 628.5 0 96 260 228 628.5 0 228 261 141 628.5 0 141 262 209 628.5 0 209 263 164 628.5 0 164 264 171 628.5 0 171 265 458 628.5 0 458 266 692 628.5 0 692 267 333 628.5 0 333 268 66 628.5 0 66 269 55 628.5 0 55 270 428 628.5 0 428 271 284 628.5 0 284 272 312 628.5 0 312 273 192 628.5 0 192 274 55 628.5 0 55 275 48 628.5 0 48 276 54 628.5 0 54 277 141 628.5 0 141 278 234 628.5 0 234 279 134 628.5 0 134 280 107 628.5 0 107 281 81 628.5 0 81 282 58 628.5 0 58 283 82 628.5 0 82 284 139 628.5 0 139 285 116 628.5 0 116 286 163 628.5 0 163 287 208 628.5 0 208 288 59 628.5 0 59 289 62 628.5 0 62 290 74 628.5 0 74 291 62 628.5 0 62 292 61 628.5 0 61 293 131 628.5 0 131 294 103 628.5 0 103 295 91 628.5 0 91 296 74 628.5 0 74 297 88 628.5 0 88 298 88 628.5 0 88 299 93 628.5 0 93 300 94 628.5 0 94 301 92 628.5 0 92 Finished in 1.34 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 29,875 Reads with adapters: 6,575 (22.0%) Reads that were too short: 103 (0.3%) Reads written (passing filters): 6,472 (21.7%) Total basepairs processed: 7,058,209 bp Total written (filtered): 1,756,988 bp (24.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6575 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 6 0.0 3 0 0 2 4 16 103 0.0 3 0 82 16 5 17 6095 0.0 3 5509 450 108 28 18 37 0.0 3 3 14 6 14 19 33 0.0 3 0 0 20 13 20 7 0.0 3 0 0 1 6 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 0 2 23 4 0.0 3 0 0 0 4 25 2 0.0 3 0 0 0 2 26 11 0.0 3 0 0 0 11 27 1 0.0 3 0 0 0 1 28 9 0.0 3 0 0 1 8 29 2 0.0 3 0 0 0 2 30 3 0.0 3 0 1 0 2 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 6 0.0 3 0 0 0 6 58 2 0.0 3 0 0 2 59 1 0.0 3 0 0 0 1 60 5 0.0 3 0 0 0 5 61 5 0.0 3 0 0 0 5 63 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 1 71 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 22 0.0 3 0 0 0 22 78 64 0.0 3 0 0 0 64 79 1 0.0 3 0 0 0 1 80 6 0.0 3 0 0 0 6 81 12 0.0 3 0 0 1 11 82 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 1 1 85 1 0.0 3 0 0 0 1 86 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 1 2 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 137 7 0.0 3 0 0 0 7 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 1 1 141 6 0.0 3 0 0 0 6 148 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 2 0.0 3 0 0 1 1 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 1 220 1 0.0 3 0 0 0 1 226 4 0.0 3 0 0 0 4 228 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 6,472 Reads with adapters: 28 (0.4%) Reads written (passing filters): 6,472 (100.0%) Total basepairs processed: 1,756,988 bp Total written (filtered): 1,756,893 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 21.4% G: 21.4% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 21 101.1 0 21 4 5 25.3 0 5 5 1 6.3 0 1 7 1 0.4 0 1