This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.63 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 224,427 (8.7%) Reads that were too short: 86,022 (3.3%) Reads written (passing filters): 138,405 (5.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 36,817,561 bp (4.8%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 224427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 983 628.5 0 983 7 26610 628.5 0 26610 8 22182 628.5 0 22182 9 780 628.5 0 780 10 1679 628.5 0 1679 11 56494 628.5 0 56494 12 125 628.5 0 125 13 97 628.5 0 97 14 75 628.5 0 75 15 98 628.5 0 98 16 114 628.5 0 114 17 112 628.5 0 112 18 56 628.5 0 56 19 49 628.5 0 49 20 75 628.5 0 75 21 60 628.5 0 60 22 52 628.5 0 52 23 82 628.5 0 82 24 70 628.5 0 70 25 75 628.5 0 75 26 58 628.5 0 58 27 64 628.5 0 64 28 55 628.5 0 55 29 67 628.5 0 67 30 80 628.5 0 80 31 79 628.5 0 79 32 76 628.5 0 76 33 69 628.5 0 69 34 73 628.5 0 73 35 79 628.5 0 79 36 73 628.5 0 73 37 75 628.5 0 75 38 80 628.5 0 80 39 71 628.5 0 71 40 83 628.5 0 83 41 65 628.5 0 65 42 61 628.5 0 61 43 122 628.5 0 122 44 63 628.5 0 63 45 93 628.5 0 93 46 81 628.5 0 81 47 60 628.5 0 60 48 71 628.5 0 71 49 63 628.5 0 63 50 83 628.5 0 83 51 67 628.5 0 67 52 116 628.5 0 116 53 160 628.5 0 160 54 223 628.5 0 223 55 127 628.5 0 127 56 83 628.5 0 83 57 98 628.5 0 98 58 88 628.5 0 88 59 124 628.5 0 124 60 141 628.5 0 141 61 84 628.5 0 84 62 88 628.5 0 88 63 90 628.5 0 90 64 115 628.5 0 115 65 124 628.5 0 124 66 77 628.5 0 77 67 94 628.5 0 94 68 107 628.5 0 107 69 89 628.5 0 89 70 86 628.5 0 86 71 114 628.5 0 114 72 330 628.5 0 330 73 200 628.5 0 200 74 259 628.5 0 259 75 105 628.5 0 105 76 54 628.5 0 54 77 88 628.5 0 88 78 102 628.5 0 102 79 103 628.5 0 103 80 90 628.5 0 90 81 110 628.5 0 110 82 97 628.5 0 97 83 86 628.5 0 86 84 101 628.5 0 101 85 96 628.5 0 96 86 101 628.5 0 101 87 89 628.5 0 89 88 89 628.5 0 89 89 92 628.5 0 92 90 83 628.5 0 83 91 93 628.5 0 93 92 67 628.5 0 67 93 86 628.5 0 86 94 75 628.5 0 75 95 89 628.5 0 89 96 81 628.5 0 81 97 84 628.5 0 84 98 94 628.5 0 94 99 86 628.5 0 86 100 94 628.5 0 94 101 80 628.5 0 80 102 162 628.5 0 162 103 211 628.5 0 211 104 207 628.5 0 207 105 121 628.5 0 121 106 130 628.5 0 130 107 130 628.5 0 130 108 91 628.5 0 91 109 103 628.5 0 103 110 111 628.5 0 111 111 124 628.5 0 124 112 110 628.5 0 110 113 103 628.5 0 103 114 98 628.5 0 98 115 100 628.5 0 100 116 109 628.5 0 109 117 99 628.5 0 99 118 89 628.5 0 89 119 86 628.5 0 86 120 104 628.5 0 104 121 130 628.5 0 130 122 150 628.5 0 150 123 138 628.5 0 138 124 272 628.5 0 272 125 277 628.5 0 277 126 291 628.5 0 291 127 123 628.5 0 123 128 101 628.5 0 101 129 120 628.5 0 120 130 136 628.5 0 136 131 123 628.5 0 123 132 388 628.5 0 388 133 329 628.5 0 329 134 170 628.5 0 170 135 239 628.5 0 239 136 96 628.5 0 96 137 162 628.5 0 162 138 140 628.5 0 140 139 139 628.5 0 139 140 145 628.5 0 145 141 119 628.5 0 119 142 111 628.5 0 111 143 109 628.5 0 109 144 142 628.5 0 142 145 180 628.5 0 180 146 228 628.5 0 228 147 534 628.5 0 534 148 265 628.5 0 265 149 393 628.5 0 393 150 205 628.5 0 205 151 170 628.5 0 170 152 164 628.5 0 164 153 163 628.5 0 163 154 156 628.5 0 156 155 127 628.5 0 127 156 292 628.5 0 292 157 250 628.5 0 250 158 338 628.5 0 338 159 216 628.5 0 216 160 207 628.5 0 207 161 213 628.5 0 213 162 243 628.5 0 243 163 199 628.5 0 199 164 162 628.5 0 162 165 174 628.5 0 174 166 215 628.5 0 215 167 217 628.5 0 217 168 215 628.5 0 215 169 1288 628.5 0 1288 170 543 628.5 0 543 171 852 628.5 0 852 172 374 628.5 0 374 173 285 628.5 0 285 174 298 628.5 0 298 175 175 628.5 0 175 176 157 628.5 0 157 177 134 628.5 0 134 178 126 628.5 0 126 179 203 628.5 0 203 180 227 628.5 0 227 181 350 628.5 0 350 182 286 628.5 0 286 183 233 628.5 0 233 184 204 628.5 0 204 185 239 628.5 0 239 186 184 628.5 0 184 187 401 628.5 0 401 188 279 628.5 0 279 189 398 628.5 0 398 190 105 628.5 0 105 191 145 628.5 0 145 192 175 628.5 0 175 193 173 628.5 0 173 194 185 628.5 0 185 195 181 628.5 0 181 196 158 628.5 0 158 197 158 628.5 0 158 198 163 628.5 0 163 199 198 628.5 0 198 200 187 628.5 0 187 201 166 628.5 0 166 202 160 628.5 0 160 203 431 628.5 0 431 204 293 628.5 0 293 205 403 628.5 0 403 206 170 628.5 0 170 207 114 628.5 0 114 208 149 628.5 0 149 209 195 628.5 0 195 210 140 628.5 0 140 211 174 628.5 0 174 212 196 628.5 0 196 213 298 628.5 0 298 214 995 628.5 0 995 215 960 628.5 0 960 216 1208 628.5 0 1208 217 818 628.5 0 818 218 309 628.5 0 309 219 432 628.5 0 432 220 248 628.5 0 248 221 266 628.5 0 266 222 281 628.5 0 281 223 337 628.5 0 337 224 459 628.5 0 459 225 922 628.5 0 922 226 4756 628.5 0 4756 227 7499 628.5 0 7499 228 8839 628.5 0 8839 229 7695 628.5 0 7695 230 1533 628.5 0 1533 231 1243 628.5 0 1243 232 915 628.5 0 915 233 612 628.5 0 612 234 172 628.5 0 172 235 139 628.5 0 139 236 179 628.5 0 179 237 334 628.5 0 334 238 350 628.5 0 350 239 419 628.5 0 419 240 260 628.5 0 260 241 139 628.5 0 139 242 132 628.5 0 132 243 120 628.5 0 120 244 181 628.5 0 181 245 236 628.5 0 236 246 198 628.5 0 198 247 217 628.5 0 217 248 360 628.5 0 360 249 285 628.5 0 285 250 217 628.5 0 217 251 151 628.5 0 151 252 128 628.5 0 128 253 151 628.5 0 151 254 227 628.5 0 227 255 288 628.5 0 288 256 389 628.5 0 389 257 587 628.5 0 587 258 454 628.5 0 454 259 468 628.5 0 468 260 362 628.5 0 362 261 398 628.5 0 398 262 335 628.5 0 335 263 531 628.5 0 531 264 2331 628.5 0 2331 265 3739 628.5 0 3739 266 5343 628.5 0 5343 267 4429 628.5 0 4429 268 2627 628.5 0 2627 269 417 628.5 0 417 270 358 628.5 0 358 271 307 628.5 0 307 272 343 628.5 0 343 273 595 628.5 0 595 274 607 628.5 0 607 275 586 628.5 0 586 276 513 628.5 0 513 277 283 628.5 0 283 278 297 628.5 0 297 279 455 628.5 0 455 280 464 628.5 0 464 281 468 628.5 0 468 282 369 628.5 0 369 283 220 628.5 0 220 284 237 628.5 0 237 285 322 628.5 0 322 286 370 628.5 0 370 287 436 628.5 0 436 288 355 628.5 0 355 289 243 628.5 0 243 290 309 628.5 0 309 291 1710 628.5 0 1710 292 2182 628.5 0 2182 293 1786 628.5 0 1786 294 342 628.5 0 342 295 189 628.5 0 189 296 161 628.5 0 161 297 137 628.5 0 137 298 142 628.5 0 142 299 177 628.5 0 177 300 212 628.5 0 212 301 504 628.5 0 504 Finished in 6.25 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 138,405 Reads with adapters: 27,791 (20.1%) Reads that were too short: 554 (0.4%) Reads written (passing filters): 27,237 (19.7%) Total basepairs processed: 36,817,561 bp Total written (filtered): 7,322,182 bp (19.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27791 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 21 0.0 3 0 0 9 12 16 229 0.0 3 0 191 25 13 17 25129 0.0 3 22671 1967 408 83 18 83 0.0 3 10 60 10 3 19 87 0.0 3 10 2 35 40 20 65 0.0 3 3 1 0 61 21 3 0.0 3 0 0 0 3 22 8 0.0 3 0 0 1 7 23 11 0.0 3 0 0 1 10 25 2 0.0 3 0 0 0 2 26 3 0.0 3 0 0 0 3 27 1 0.0 3 1 29 2 0.0 3 2 30 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 33 2 0.0 3 0 0 1 1 34 2 0.0 3 1 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 42 4 0.0 3 0 0 0 4 44 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 8 0.0 3 0 0 0 8 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 1 57 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 5 0.0 3 0 0 0 5 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 67 11 0.0 3 0 0 1 10 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 6 0.0 3 0 0 0 6 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 1 73 2 0.0 3 0 0 0 2 74 7 0.0 3 0 0 0 7 75 1 0.0 3 0 0 0 1 76 3 0.0 3 1 0 1 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 1 80 2 0.0 3 1 0 0 1 81 3 0.0 3 1 1 1 82 2 0.0 3 1 0 0 1 89 2 0.0 3 0 0 0 2 91 28 0.0 3 0 0 0 28 92 4 0.0 3 0 0 0 4 93 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 0 4 95 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 1 2 108 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 18 0.0 3 0 0 1 17 114 9 0.0 3 0 0 0 9 115 2 0.0 3 0 0 0 2 116 1 0.0 3 1 117 1 0.0 3 0 0 1 118 4 0.0 3 0 1 0 3 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 5 0.0 3 0 0 0 5 127 565 0.0 3 0 0 3 562 128 5 0.0 3 0 0 1 4 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 4 0.0 3 0 0 0 4 133 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 155 5 0.0 3 0 0 0 5 156 2 0.0 3 0 0 0 2 157 34 0.0 3 0 0 0 34 158 4 0.0 3 0 0 0 4 159 6 0.0 3 0 0 0 6 160 9 0.0 3 0 0 0 9 161 7 0.0 3 0 0 2 5 162 218 0.0 3 0 0 5 213 163 19 0.0 3 0 0 0 19 164 41 0.0 3 0 0 0 41 165 38 0.0 3 0 0 1 37 166 3 0.0 3 0 0 0 3 167 3 0.0 3 0 0 0 3 168 10 0.0 3 0 0 3 7 169 1 0.0 3 0 0 0 1 171 11 0.0 3 0 0 0 11 172 9 0.0 3 0 0 1 8 173 9 0.0 3 0 0 0 9 174 12 0.0 3 0 0 1 11 175 6 0.0 3 0 0 1 5 176 6 0.0 3 0 0 0 6 177 7 0.0 3 0 0 0 7 178 3 0.0 3 0 0 0 3 179 11 0.0 3 0 0 0 11 180 2 0.0 3 0 0 1 1 181 16 0.0 3 0 0 0 16 182 198 0.0 3 0 0 3 195 183 95 0.0 3 0 0 1 94 184 2 0.0 3 0 0 0 2 185 29 0.0 3 0 0 2 27 186 19 0.0 3 0 0 0 19 187 4 0.0 3 0 0 0 4 188 8 0.0 3 0 0 1 7 189 1 0.0 3 0 0 0 1 190 6 0.0 3 0 0 0 6 192 2 0.0 3 0 0 0 2 194 5 0.0 3 0 0 0 5 198 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 1 203 2 0.0 3 0 0 0 2 204 4 0.0 3 0 0 0 4 205 4 0.0 3 0 0 0 4 206 21 0.0 3 0 0 0 21 207 2 0.0 3 0 0 0 2 208 13 0.0 3 0 0 2 11 209 7 0.0 3 0 0 0 7 213 4 0.0 3 0 0 0 4 215 6 0.0 3 0 0 0 6 217 98 0.0 3 0 0 2 96 218 6 0.0 3 0 0 0 6 220 3 0.0 3 0 0 0 3 221 4 0.0 3 0 0 0 4 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 226 2 0.0 3 0 0 0 2 227 2 0.0 3 0 0 0 2 230 2 0.0 3 0 0 0 2 233 15 0.0 3 0 0 1 14 234 9 0.0 3 0 0 1 8 235 6 0.0 3 0 0 0 6 238 2 0.0 3 0 0 0 2 240 1 0.0 3 0 0 0 1 241 3 0.0 3 0 0 1 2 242 9 0.0 3 0 0 0 9 243 5 0.0 3 0 0 0 5 244 1 0.0 3 0 0 0 1 245 2 0.0 3 0 1 0 1 246 2 0.0 3 0 0 0 2 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 4 0.0 3 0 0 0 4 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 264 2 0.0 3 0 0 1 1 265 2 0.0 3 0 0 0 2 266 2 0.0 3 0 0 0 2 267 50 0.0 3 0 0 3 47 268 5 0.0 3 0 0 1 4 269 5 0.0 3 0 0 0 5 270 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 273 2 0.0 3 0 0 0 2 274 18 0.0 3 0 0 0 18 275 3 0.0 3 0 0 0 3 276 8 0.0 3 0 0 0 8 277 7 0.0 3 0 0 1 6 278 5 0.0 3 0 0 0 5 279 5 0.0 3 0 0 0 5 281 6 0.0 3 0 0 1 5 282 8 0.0 3 0 0 0 8 283 26 0.0 3 0 0 0 26 284 31 0.0 3 0 0 2 29 285 2 0.0 3 0 0 0 2 286 4 0.0 3 0 0 0 4 287 3 0.0 3 0 0 0 3 289 2 0.0 3 0 0 0 2 290 5 0.0 3 0 0 0 5 292 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 27,237 Reads with adapters: 70 (0.3%) Reads written (passing filters): 27,237 (100.0%) Total basepairs processed: 7,322,182 bp Total written (filtered): 7,321,967 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 70 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.6% C: 44.3% G: 21.4% T: 5.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 66 425.6 0 66 4 3 106.4 0 3 5 1 26.6 0 1