This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.89 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 171,762 (6.7%) Reads that were too short: 39,011 (1.5%) Reads written (passing filters): 132,751 (5.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 36,258,954 bp (4.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 171762 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 689 628.5 0 689 7 23820 628.5 0 23820 8 27776 628.5 0 27776 9 504 628.5 0 504 10 1012 628.5 0 1012 11 56657 628.5 0 56657 12 123 628.5 0 123 13 80 628.5 0 80 14 100 628.5 0 100 15 84 628.5 0 84 16 76 628.5 0 76 17 84 628.5 0 84 18 71 628.5 0 71 19 77 628.5 0 77 20 83 628.5 0 83 21 52 628.5 0 52 22 91 628.5 0 91 23 64 628.5 0 64 24 99 628.5 0 99 25 86 628.5 0 86 26 75 628.5 0 75 27 74 628.5 0 74 28 71 628.5 0 71 29 82 628.5 0 82 30 59 628.5 0 59 31 118 628.5 0 118 32 102 628.5 0 102 33 69 628.5 0 69 34 90 628.5 0 90 35 98 628.5 0 98 36 84 628.5 0 84 37 79 628.5 0 79 38 102 628.5 0 102 39 72 628.5 0 72 40 58 628.5 0 58 41 60 628.5 0 60 42 59 628.5 0 59 43 54 628.5 0 54 44 62 628.5 0 62 45 96 628.5 0 96 46 74 628.5 0 74 47 65 628.5 0 65 48 66 628.5 0 66 49 77 628.5 0 77 50 74 628.5 0 74 51 70 628.5 0 70 52 107 628.5 0 107 53 117 628.5 0 117 54 92 628.5 0 92 55 53 628.5 0 53 56 95 628.5 0 95 57 88 628.5 0 88 58 67 628.5 0 67 59 89 628.5 0 89 60 118 628.5 0 118 61 90 628.5 0 90 62 76 628.5 0 76 63 78 628.5 0 78 64 128 628.5 0 128 65 94 628.5 0 94 66 101 628.5 0 101 67 71 628.5 0 71 68 81 628.5 0 81 69 71 628.5 0 71 70 93 628.5 0 93 71 101 628.5 0 101 72 226 628.5 0 226 73 111 628.5 0 111 74 164 628.5 0 164 75 106 628.5 0 106 76 66 628.5 0 66 77 82 628.5 0 82 78 114 628.5 0 114 79 107 628.5 0 107 80 117 628.5 0 117 81 148 628.5 0 148 82 123 628.5 0 123 83 144 628.5 0 144 84 106 628.5 0 106 85 109 628.5 0 109 86 129 628.5 0 129 87 117 628.5 0 117 88 108 628.5 0 108 89 80 628.5 0 80 90 98 628.5 0 98 91 85 628.5 0 85 92 90 628.5 0 90 93 96 628.5 0 96 94 78 628.5 0 78 95 81 628.5 0 81 96 94 628.5 0 94 97 99 628.5 0 99 98 90 628.5 0 90 99 138 628.5 0 138 100 110 628.5 0 110 101 78 628.5 0 78 102 100 628.5 0 100 103 140 628.5 0 140 104 118 628.5 0 118 105 87 628.5 0 87 106 101 628.5 0 101 107 113 628.5 0 113 108 94 628.5 0 94 109 87 628.5 0 87 110 112 628.5 0 112 111 75 628.5 0 75 112 82 628.5 0 82 113 82 628.5 0 82 114 83 628.5 0 83 115 94 628.5 0 94 116 129 628.5 0 129 117 103 628.5 0 103 118 96 628.5 0 96 119 90 628.5 0 90 120 96 628.5 0 96 121 121 628.5 0 121 122 101 628.5 0 101 123 99 628.5 0 99 124 110 628.5 0 110 125 136 628.5 0 136 126 140 628.5 0 140 127 104 628.5 0 104 128 87 628.5 0 87 129 111 628.5 0 111 130 107 628.5 0 107 131 122 628.5 0 122 132 320 628.5 0 320 133 161 628.5 0 161 134 118 628.5 0 118 135 111 628.5 0 111 136 129 628.5 0 129 137 134 628.5 0 134 138 118 628.5 0 118 139 131 628.5 0 131 140 104 628.5 0 104 141 82 628.5 0 82 142 105 628.5 0 105 143 78 628.5 0 78 144 100 628.5 0 100 145 127 628.5 0 127 146 158 628.5 0 158 147 370 628.5 0 370 148 141 628.5 0 141 149 196 628.5 0 196 150 109 628.5 0 109 151 100 628.5 0 100 152 114 628.5 0 114 153 101 628.5 0 101 154 104 628.5 0 104 155 87 628.5 0 87 156 302 628.5 0 302 157 179 628.5 0 179 158 351 628.5 0 351 159 98 628.5 0 98 160 133 628.5 0 133 161 83 628.5 0 83 162 125 628.5 0 125 163 117 628.5 0 117 164 112 628.5 0 112 165 116 628.5 0 116 166 137 628.5 0 137 167 123 628.5 0 123 168 125 628.5 0 125 169 852 628.5 0 852 170 239 628.5 0 239 171 432 628.5 0 432 172 187 628.5 0 187 173 121 628.5 0 121 174 155 628.5 0 155 175 116 628.5 0 116 176 98 628.5 0 98 177 120 628.5 0 120 178 116 628.5 0 116 179 103 628.5 0 103 180 128 628.5 0 128 181 115 628.5 0 115 182 125 628.5 0 125 183 122 628.5 0 122 184 118 628.5 0 118 185 130 628.5 0 130 186 116 628.5 0 116 187 184 628.5 0 184 188 223 628.5 0 223 189 233 628.5 0 233 190 144 628.5 0 144 191 103 628.5 0 103 192 141 628.5 0 141 193 155 628.5 0 155 194 110 628.5 0 110 195 174 628.5 0 174 196 141 628.5 0 141 197 135 628.5 0 135 198 121 628.5 0 121 199 126 628.5 0 126 200 118 628.5 0 118 201 109 628.5 0 109 202 131 628.5 0 131 203 214 628.5 0 214 204 151 628.5 0 151 205 169 628.5 0 169 206 110 628.5 0 110 207 112 628.5 0 112 208 95 628.5 0 95 209 106 628.5 0 106 210 106 628.5 0 106 211 127 628.5 0 127 212 128 628.5 0 128 213 171 628.5 0 171 214 538 628.5 0 538 215 481 628.5 0 481 216 637 628.5 0 637 217 353 628.5 0 353 218 153 628.5 0 153 219 165 628.5 0 165 220 129 628.5 0 129 221 120 628.5 0 120 222 135 628.5 0 135 223 177 628.5 0 177 224 229 628.5 0 229 225 366 628.5 0 366 226 2213 628.5 0 2213 227 3475 628.5 0 3475 228 3065 628.5 0 3065 229 4148 628.5 0 4148 230 1134 628.5 0 1134 231 1090 628.5 0 1090 232 857 628.5 0 857 233 690 628.5 0 690 234 110 628.5 0 110 235 83 628.5 0 83 236 107 628.5 0 107 237 153 628.5 0 153 238 178 628.5 0 178 239 195 628.5 0 195 240 127 628.5 0 127 241 113 628.5 0 113 242 105 628.5 0 105 243 90 628.5 0 90 244 105 628.5 0 105 245 175 628.5 0 175 246 143 628.5 0 143 247 133 628.5 0 133 248 218 628.5 0 218 249 182 628.5 0 182 250 153 628.5 0 153 251 130 628.5 0 130 252 115 628.5 0 115 253 94 628.5 0 94 254 129 628.5 0 129 255 126 628.5 0 126 256 150 628.5 0 150 257 181 628.5 0 181 258 143 628.5 0 143 259 178 628.5 0 178 260 165 628.5 0 165 261 240 628.5 0 240 262 181 628.5 0 181 263 208 628.5 0 208 264 792 628.5 0 792 265 1179 628.5 0 1179 266 1696 628.5 0 1696 267 1357 628.5 0 1357 268 866 628.5 0 866 269 180 628.5 0 180 270 188 628.5 0 188 271 151 628.5 0 151 272 129 628.5 0 129 273 222 628.5 0 222 274 274 628.5 0 274 275 245 628.5 0 245 276 303 628.5 0 303 277 154 628.5 0 154 278 169 628.5 0 169 279 219 628.5 0 219 280 209 628.5 0 209 281 203 628.5 0 203 282 194 628.5 0 194 283 181 628.5 0 181 284 149 628.5 0 149 285 128 628.5 0 128 286 176 628.5 0 176 287 195 628.5 0 195 288 170 628.5 0 170 289 129 628.5 0 129 290 148 628.5 0 148 291 616 628.5 0 616 292 643 628.5 0 643 293 524 628.5 0 524 294 163 628.5 0 163 295 126 628.5 0 126 296 108 628.5 0 108 297 108 628.5 0 108 298 100 628.5 0 100 299 125 628.5 0 125 300 120 628.5 0 120 301 187 628.5 0 187 Finished in 7.10 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 132,751 Reads with adapters: 36,118 (27.2%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 36,093 (27.2%) Total basepairs processed: 36,258,954 bp Total written (filtered): 8,979,812 bp (24.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 36118 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 8 19 386 0.0 3 0 374 10 2 20 27507 0.0 4 27077 410 18 1 1 21 63 0.0 4 11 50 2 22 4 0.0 4 0 0 4 23 10 0.0 4 0 0 0 10 24 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 85 1 0.0 4 1 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 1 97 1 0.0 4 0 0 0 1 101 1 0.0 4 1 107 1 0.0 4 1 114 1 0.0 4 1 117 2 0.0 4 0 0 0 0 2 118 2 0.0 4 2 119 1 0.0 4 0 1 120 1 0.0 4 1 121 3 0.0 4 1 2 122 2 0.0 4 0 0 0 0 2 123 23 0.0 4 0 0 0 0 23 124 47 0.0 4 0 0 0 0 47 125 7672 0.0 4 0 0 0 0 7672 126 24 0.0 4 0 0 0 0 24 127 326 0.0 4 0 0 0 0 326 128 9 0.0 4 0 0 0 0 9 143 1 0.0 4 1 148 1 0.0 4 1 213 1 0.0 4 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 1 280 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 36,093 Reads with adapters: 3,370 (9.3%) Reads written (passing filters): 36,093 (100.0%) Total basepairs processed: 8,979,812 bp Total written (filtered): 8,942,527 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3370 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 2.9% G: 92.9% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 564.0 0 16 4 4 141.0 0 4 6 1 8.8 0 1 7 2 2.2 0 2 8 1 0.6 0 1 9 45 0.1 0 3 42 10 344 0.0 1 9 335 11 2915 0.0 1 16 2899 12 7 0.0 1 0 7 13 2 0.0 1 0 2 14 1 0.0 1 0 1 19 24 0.0 1 20 4 20 4 0.0 1 4 151 3 0.0 1 0 3 170 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.