This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.25 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 54,734 (2.1%) Reads that were too short: 8,814 (0.3%) Reads written (passing filters): 45,920 (1.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,359,568 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 54734 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 713 628.5 0 713 7 12884 628.5 0 12884 8 491 628.5 0 491 9 12989 628.5 0 12989 10 11 628.5 0 11 11 5 628.5 0 5 12 3 628.5 0 3 13 5 628.5 0 5 14 3 628.5 0 3 15 5 628.5 0 5 16 1 628.5 0 1 17 2 628.5 0 2 18 2 628.5 0 2 19 3 628.5 0 3 20 4 628.5 0 4 21 10 628.5 0 10 22 15 628.5 0 15 23 35 628.5 0 35 24 5 628.5 0 5 25 74 628.5 0 74 26 11 628.5 0 11 27 19 628.5 0 19 28 3 628.5 0 3 29 4 628.5 0 4 30 7 628.5 0 7 31 5 628.5 0 5 32 8 628.5 0 8 33 19 628.5 0 19 34 103 628.5 0 103 35 248 628.5 0 248 36 6 628.5 0 6 37 7 628.5 0 7 38 6 628.5 0 6 39 4 628.5 0 4 40 13 628.5 0 13 41 7 628.5 0 7 42 15 628.5 0 15 43 3 628.5 0 3 44 3 628.5 0 3 45 2 628.5 0 2 46 3 628.5 0 3 48 8 628.5 0 8 49 4 628.5 0 4 50 6 628.5 0 6 51 4 628.5 0 4 52 7 628.5 0 7 53 3 628.5 0 3 54 2 628.5 0 2 55 2 628.5 0 2 56 4 628.5 0 4 57 3 628.5 0 3 58 11 628.5 0 11 59 5 628.5 0 5 60 7 628.5 0 7 61 6 628.5 0 6 62 10 628.5 0 10 63 2 628.5 0 2 64 5 628.5 0 5 65 7 628.5 0 7 66 12 628.5 0 12 67 5 628.5 0 5 68 9 628.5 0 9 69 7 628.5 0 7 70 8 628.5 0 8 71 5 628.5 0 5 72 3 628.5 0 3 73 10 628.5 0 10 74 31 628.5 0 31 75 10 628.5 0 10 76 18 628.5 0 18 77 25 628.5 0 25 78 54 628.5 0 54 79 54 628.5 0 54 80 16 628.5 0 16 81 35 628.5 0 35 82 19 628.5 0 19 83 28 628.5 0 28 84 13 628.5 0 13 85 17 628.5 0 17 86 32 628.5 0 32 87 24 628.5 0 24 88 11 628.5 0 11 89 34 628.5 0 34 90 28 628.5 0 28 91 14 628.5 0 14 92 10 628.5 0 10 93 32 628.5 0 32 94 34 628.5 0 34 95 26 628.5 0 26 96 54 628.5 0 54 97 105 628.5 0 105 98 114 628.5 0 114 99 923 628.5 0 923 100 862 628.5 0 862 101 1367 628.5 0 1367 102 59 628.5 0 59 103 21 628.5 0 21 104 23 628.5 0 23 105 16 628.5 0 16 106 33 628.5 0 33 107 45 628.5 0 45 108 50 628.5 0 50 109 64 628.5 0 64 110 93 628.5 0 93 111 886 628.5 0 886 112 1494 628.5 0 1494 113 2113 628.5 0 2113 114 458 628.5 0 458 115 1106 628.5 0 1106 116 16 628.5 0 16 117 15 628.5 0 15 118 12 628.5 0 12 119 29 628.5 0 29 120 16 628.5 0 16 121 12 628.5 0 12 122 14 628.5 0 14 123 17 628.5 0 17 124 17 628.5 0 17 125 15 628.5 0 15 126 25 628.5 0 25 127 17 628.5 0 17 128 29 628.5 0 29 129 178 628.5 0 178 130 365 628.5 0 365 131 161 628.5 0 161 132 70 628.5 0 70 133 35 628.5 0 35 134 44 628.5 0 44 135 208 628.5 0 208 136 225 628.5 0 225 137 156 628.5 0 156 138 75 628.5 0 75 139 67 628.5 0 67 140 43 628.5 0 43 141 72 628.5 0 72 142 542 628.5 0 542 143 1215 628.5 0 1215 144 1429 628.5 0 1429 145 597 628.5 0 597 146 235 628.5 0 235 147 407 628.5 0 407 148 22 628.5 0 22 149 22 628.5 0 22 150 19 628.5 0 19 151 16 628.5 0 16 152 22 628.5 0 22 153 14 628.5 0 14 154 10 628.5 0 10 155 18 628.5 0 18 156 33 628.5 0 33 157 30 628.5 0 30 158 47 628.5 0 47 159 34 628.5 0 34 160 54 628.5 0 54 161 47 628.5 0 47 162 38 628.5 0 38 163 19 628.5 0 19 164 19 628.5 0 19 165 16 628.5 0 16 166 32 628.5 0 32 167 25 628.5 0 25 168 47 628.5 0 47 169 15 628.5 0 15 170 16 628.5 0 16 171 10 628.5 0 10 172 10 628.5 0 10 173 10 628.5 0 10 174 14 628.5 0 14 175 30 628.5 0 30 176 20 628.5 0 20 177 32 628.5 0 32 178 32 628.5 0 32 179 23 628.5 0 23 180 27 628.5 0 27 181 19 628.5 0 19 182 26 628.5 0 26 183 22 628.5 0 22 184 19 628.5 0 19 185 13 628.5 0 13 186 24 628.5 0 24 187 15 628.5 0 15 188 13 628.5 0 13 189 11 628.5 0 11 190 43 628.5 0 43 191 21 628.5 0 21 192 6 628.5 0 6 193 15 628.5 0 15 194 19 628.5 0 19 195 19 628.5 0 19 196 20 628.5 0 20 197 16 628.5 0 16 198 17 628.5 0 17 199 11 628.5 0 11 200 12 628.5 0 12 201 16 628.5 0 16 202 25 628.5 0 25 203 28 628.5 0 28 204 22 628.5 0 22 205 26 628.5 0 26 206 21 628.5 0 21 207 24 628.5 0 24 208 28 628.5 0 28 209 29 628.5 0 29 210 19 628.5 0 19 211 28 628.5 0 28 212 15 628.5 0 15 213 25 628.5 0 25 214 26 628.5 0 26 215 20 628.5 0 20 216 35 628.5 0 35 217 40 628.5 0 40 218 28 628.5 0 28 219 27 628.5 0 27 220 27 628.5 0 27 221 32 628.5 0 32 222 52 628.5 0 52 223 75 628.5 0 75 224 121 628.5 0 121 225 57 628.5 0 57 226 85 628.5 0 85 227 26 628.5 0 26 228 25 628.5 0 25 229 28 628.5 0 28 230 27 628.5 0 27 231 22 628.5 0 22 232 28 628.5 0 28 233 62 628.5 0 62 234 49 628.5 0 49 235 32 628.5 0 32 236 39 628.5 0 39 237 31 628.5 0 31 238 47 628.5 0 47 239 36 628.5 0 36 240 39 628.5 0 39 241 93 628.5 0 93 242 97 628.5 0 97 243 129 628.5 0 129 244 72 628.5 0 72 245 45 628.5 0 45 246 24 628.5 0 24 247 30 628.5 0 30 248 25 628.5 0 25 249 17 628.5 0 17 250 23 628.5 0 23 251 36 628.5 0 36 252 52 628.5 0 52 253 39 628.5 0 39 254 56 628.5 0 56 255 60 628.5 0 60 256 83 628.5 0 83 257 91 628.5 0 91 258 73 628.5 0 73 259 70 628.5 0 70 260 46 628.5 0 46 261 50 628.5 0 50 262 149 628.5 0 149 263 217 628.5 0 217 264 242 628.5 0 242 265 151 628.5 0 151 266 32 628.5 0 32 267 33 628.5 0 33 268 37 628.5 0 37 269 33 628.5 0 33 270 51 628.5 0 51 271 50 628.5 0 50 272 50 628.5 0 50 273 57 628.5 0 57 274 39 628.5 0 39 275 79 628.5 0 79 276 102 628.5 0 102 277 224 628.5 0 224 278 174 628.5 0 174 279 67 628.5 0 67 280 78 628.5 0 78 281 76 628.5 0 76 282 583 628.5 0 583 283 194 628.5 0 194 284 393 628.5 0 393 285 124 628.5 0 124 286 53 628.5 0 53 287 46 628.5 0 46 288 48 628.5 0 48 289 44 628.5 0 44 290 118 628.5 0 118 291 190 628.5 0 190 292 269 628.5 0 269 293 123 628.5 0 123 294 631 628.5 0 631 295 831 628.5 0 831 296 332 628.5 0 332 297 57 628.5 0 57 298 61 628.5 0 61 299 66 628.5 0 66 300 60 628.5 0 60 301 33 628.5 0 33 Finished in 2.06 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 45,920 Reads with adapters: 13,936 (30.3%) Reads that were too short: 114 (0.2%) Reads written (passing filters): 13,822 (30.1%) Total basepairs processed: 11,359,568 bp Total written (filtered): 3,768,024 bp (33.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13936 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 26 0.0 3 0 0 19 7 16 281 0.0 3 0 233 35 13 17 13135 0.0 3 11838 1037 210 50 18 41 0.0 3 2 23 11 5 19 61 0.0 3 4 1 25 31 20 39 0.0 3 2 1 3 33 21 2 0.0 3 0 0 0 2 22 2 0.0 3 0 0 0 2 23 8 0.0 3 1 0 0 7 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 29 78 0.0 3 0 0 2 76 32 5 0.0 3 0 0 0 5 35 2 0.0 3 0 0 0 2 37 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 1 3 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 13 0.0 3 0 0 0 13 64 2 0.0 3 0 0 1 1 67 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 72 33 0.0 3 0 0 0 33 73 7 0.0 3 0 0 0 7 74 5 0.0 3 0 0 1 4 75 4 0.0 3 0 0 0 4 76 4 0.0 3 0 0 0 4 80 5 0.0 3 0 0 0 5 82 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 92 8 0.0 3 0 0 1 7 93 8 0.0 3 0 0 0 8 96 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 5 0.0 3 0 0 0 5 100 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 0 4 133 1 0.0 3 0 0 0 1 141 12 0.0 3 0 0 1 11 142 2 0.0 3 0 0 0 2 143 3 0.0 3 0 0 0 3 144 10 0.0 3 0 0 0 10 145 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 3 0.0 3 0 0 0 3 194 3 0.0 3 0 0 0 3 195 3 0.0 3 0 0 0 3 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 17 0.0 3 0 0 1 16 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 13,822 Reads with adapters: 227 (1.6%) Reads written (passing filters): 13,822 (100.0%) Total basepairs processed: 3,768,024 bp Total written (filtered): 3,767,328 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 227 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.9% C: 16.7% G: 48.0% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 213 216.0 0 213 4 13 54.0 0 13 5 1 13.5 0 1