This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.57 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 52,736 (2.0%) Reads that were too short: 7,206 (0.3%) Reads written (passing filters): 45,530 (1.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,484,371 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 52736 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 809 628.5 0 809 7 15550 628.5 0 15550 8 398 628.5 0 398 9 12320 628.5 0 12320 10 9 628.5 0 9 11 1 628.5 0 1 12 1 628.5 0 1 13 2 628.5 0 2 15 2 628.5 0 2 17 2 628.5 0 2 18 2 628.5 0 2 20 1 628.5 0 1 22 2 628.5 0 2 24 2 628.5 0 2 25 1 628.5 0 1 28 1 628.5 0 1 29 1 628.5 0 1 30 7 628.5 0 7 31 2 628.5 0 2 32 1 628.5 0 1 33 4 628.5 0 4 35 4 628.5 0 4 37 3 628.5 0 3 38 2 628.5 0 2 39 10 628.5 0 10 40 10 628.5 0 10 41 774 628.5 0 774 42 74 628.5 0 74 43 5 628.5 0 5 46 4 628.5 0 4 47 3 628.5 0 3 48 7 628.5 0 7 49 4 628.5 0 4 50 2 628.5 0 2 51 1 628.5 0 1 52 5 628.5 0 5 53 2 628.5 0 2 54 6 628.5 0 6 56 2 628.5 0 2 57 2 628.5 0 2 58 3 628.5 0 3 59 4 628.5 0 4 60 7 628.5 0 7 61 4 628.5 0 4 62 2 628.5 0 2 64 2 628.5 0 2 65 4 628.5 0 4 66 7 628.5 0 7 67 5 628.5 0 5 68 5 628.5 0 5 70 4 628.5 0 4 71 6 628.5 0 6 72 2 628.5 0 2 73 4 628.5 0 4 74 5 628.5 0 5 75 5 628.5 0 5 76 7 628.5 0 7 77 14 628.5 0 14 78 42 628.5 0 42 79 20 628.5 0 20 80 8 628.5 0 8 81 13 628.5 0 13 82 16 628.5 0 16 83 11 628.5 0 11 84 8 628.5 0 8 85 6 628.5 0 6 86 18 628.5 0 18 87 10 628.5 0 10 88 11 628.5 0 11 89 13 628.5 0 13 90 12 628.5 0 12 91 12 628.5 0 12 92 15 628.5 0 15 93 9 628.5 0 9 94 14 628.5 0 14 95 25 628.5 0 25 96 32 628.5 0 32 97 34 628.5 0 34 98 61 628.5 0 61 99 246 628.5 0 246 100 310 628.5 0 310 101 294 628.5 0 294 102 37 628.5 0 37 103 20 628.5 0 20 104 26 628.5 0 26 105 23 628.5 0 23 106 30 628.5 0 30 107 49 628.5 0 49 108 43 628.5 0 43 109 77 628.5 0 77 110 61 628.5 0 61 111 888 628.5 0 888 112 1611 628.5 0 1611 113 2259 628.5 0 2259 114 514 628.5 0 514 115 1272 628.5 0 1272 116 15 628.5 0 15 117 10 628.5 0 10 118 8 628.5 0 8 119 30 628.5 0 30 120 14 628.5 0 14 121 6 628.5 0 6 122 14 628.5 0 14 123 12 628.5 0 12 124 10 628.5 0 10 125 20 628.5 0 20 126 18 628.5 0 18 127 17 628.5 0 17 128 21 628.5 0 21 129 118 628.5 0 118 130 271 628.5 0 271 131 112 628.5 0 112 132 36 628.5 0 36 133 30 628.5 0 30 134 25 628.5 0 25 135 219 628.5 0 219 136 211 628.5 0 211 137 93 628.5 0 93 138 37 628.5 0 37 139 56 628.5 0 56 140 50 628.5 0 50 141 50 628.5 0 50 142 701 628.5 0 701 143 1499 628.5 0 1499 144 1898 628.5 0 1898 145 645 628.5 0 645 146 82 628.5 0 82 147 137 628.5 0 137 148 7 628.5 0 7 149 13 628.5 0 13 150 15 628.5 0 15 151 24 628.5 0 24 152 14 628.5 0 14 153 11 628.5 0 11 154 8 628.5 0 8 155 10 628.5 0 10 156 11 628.5 0 11 157 16 628.5 0 16 158 16 628.5 0 16 159 25 628.5 0 25 160 29 628.5 0 29 161 35 628.5 0 35 162 22 628.5 0 22 163 14 628.5 0 14 164 9 628.5 0 9 165 9 628.5 0 9 166 15 628.5 0 15 167 17 628.5 0 17 168 18 628.5 0 18 169 10 628.5 0 10 170 13 628.5 0 13 171 10 628.5 0 10 172 10 628.5 0 10 173 10 628.5 0 10 174 9 628.5 0 9 175 15 628.5 0 15 176 10 628.5 0 10 177 24 628.5 0 24 178 12 628.5 0 12 179 19 628.5 0 19 180 11 628.5 0 11 181 5 628.5 0 5 182 12 628.5 0 12 183 5 628.5 0 5 184 9 628.5 0 9 185 10 628.5 0 10 186 14 628.5 0 14 187 13 628.5 0 13 188 16 628.5 0 16 189 20 628.5 0 20 190 54 628.5 0 54 191 6 628.5 0 6 192 8 628.5 0 8 193 19 628.5 0 19 194 9 628.5 0 9 195 10 628.5 0 10 196 15 628.5 0 15 197 19 628.5 0 19 198 7 628.5 0 7 199 16 628.5 0 16 200 13 628.5 0 13 201 16 628.5 0 16 202 24 628.5 0 24 203 16 628.5 0 16 204 17 628.5 0 17 205 13 628.5 0 13 206 22 628.5 0 22 207 21 628.5 0 21 208 21 628.5 0 21 209 21 628.5 0 21 210 19 628.5 0 19 211 16 628.5 0 16 212 15 628.5 0 15 213 17 628.5 0 17 214 14 628.5 0 14 215 17 628.5 0 17 216 9 628.5 0 9 217 18 628.5 0 18 218 26 628.5 0 26 219 21 628.5 0 21 220 24 628.5 0 24 221 34 628.5 0 34 222 28 628.5 0 28 223 37 628.5 0 37 224 146 628.5 0 146 225 45 628.5 0 45 226 97 628.5 0 97 227 16 628.5 0 16 228 12 628.5 0 12 229 24 628.5 0 24 230 21 628.5 0 21 231 16 628.5 0 16 232 23 628.5 0 23 233 32 628.5 0 32 234 20 628.5 0 20 235 21 628.5 0 21 236 26 628.5 0 26 237 25 628.5 0 25 238 19 628.5 0 19 239 31 628.5 0 31 240 24 628.5 0 24 241 36 628.5 0 36 242 45 628.5 0 45 243 51 628.5 0 51 244 36 628.5 0 36 245 25 628.5 0 25 246 18 628.5 0 18 247 22 628.5 0 22 248 11 628.5 0 11 249 26 628.5 0 26 250 22 628.5 0 22 251 34 628.5 0 34 252 31 628.5 0 31 253 24 628.5 0 24 254 31 628.5 0 31 255 29 628.5 0 29 256 34 628.5 0 34 257 44 628.5 0 44 258 37 628.5 0 37 259 39 628.5 0 39 260 61 628.5 0 61 261 27 628.5 0 27 262 58 628.5 0 58 263 71 628.5 0 71 264 77 628.5 0 77 265 38 628.5 0 38 266 19 628.5 0 19 267 18 628.5 0 18 268 22 628.5 0 22 269 22 628.5 0 22 270 36 628.5 0 36 271 34 628.5 0 34 272 26 628.5 0 26 273 38 628.5 0 38 274 26 628.5 0 26 275 30 628.5 0 30 276 88 628.5 0 88 277 160 628.5 0 160 278 83 628.5 0 83 279 43 628.5 0 43 280 36 628.5 0 36 281 53 628.5 0 53 282 345 628.5 0 345 283 116 628.5 0 116 284 184 628.5 0 184 285 62 628.5 0 62 286 35 628.5 0 35 287 26 628.5 0 26 288 40 628.5 0 40 289 49 628.5 0 49 290 127 628.5 0 127 291 167 628.5 0 167 292 377 628.5 0 377 293 134 628.5 0 134 294 886 628.5 0 886 295 1269 628.5 0 1269 296 485 628.5 0 485 297 50 628.5 0 50 298 38 628.5 0 38 299 46 628.5 0 46 300 34 628.5 0 34 301 27 628.5 0 27 Finished in 2.25 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 45,530 Reads with adapters: 16,383 (36.0%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 16,367 (35.9%) Total basepairs processed: 11,484,371 bp Total written (filtered): 4,465,945 bp (38.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16383 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 155 0.0 3 0 149 6 20 15965 0.0 4 15706 245 13 1 21 29 0.0 4 11 18 22 25 0.0 4 24 1 23 10 0.0 4 0 0 0 10 25 1 0.0 4 1 35 18 0.0 4 0 0 0 0 18 39 1 0.0 4 1 85 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 1 122 3 0.0 4 0 0 0 0 3 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 154 0.0 4 0 0 0 0 154 128 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 1 147 1 0.0 4 1 226 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 16,367 Reads with adapters: 362 (2.2%) Reads written (passing filters): 16,367 (100.0%) Total basepairs processed: 4,465,945 bp Total written (filtered): 4,461,341 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 362 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 19.1% C: 6.4% G: 73.2% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 44 255.7 0 44 4 2 63.9 0 2 10 3 0.0 1 0 3 11 243 0.0 1 1 242 12 34 0.0 1 1 33 13 1 0.0 1 0 1 15 2 0.0 1 0 2 20 1 0.0 1 1 24 27 0.0 1 24 3 25 1 0.0 1 1 147 2 0.0 1 2 152 1 0.0 1 0 1 171 1 0.0 1 1