This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.67 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 131,372 (5.1%) Reads that were too short: 15,379 (0.6%) Reads written (passing filters): 115,993 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 31,090,253 bp (4.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 131372 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1225 628.5 0 1225 7 48486 628.5 0 48486 8 1872 628.5 0 1872 9 37852 628.5 0 37852 10 91 628.5 0 91 11 55 628.5 0 55 12 84 628.5 0 84 13 96 628.5 0 96 14 77 628.5 0 77 15 54 628.5 0 54 16 73 628.5 0 73 17 72 628.5 0 72 18 57 628.5 0 57 19 41 628.5 0 41 20 48 628.5 0 48 21 38 628.5 0 38 22 48 628.5 0 48 23 36 628.5 0 36 24 45 628.5 0 45 25 51 628.5 0 51 26 49 628.5 0 49 27 65 628.5 0 65 28 91 628.5 0 91 29 80 628.5 0 80 30 79 628.5 0 79 31 71 628.5 0 71 32 59 628.5 0 59 33 73 628.5 0 73 34 55 628.5 0 55 35 55 628.5 0 55 36 68 628.5 0 68 37 73 628.5 0 73 38 68 628.5 0 68 39 55 628.5 0 55 40 91 628.5 0 91 41 74 628.5 0 74 42 73 628.5 0 73 43 73 628.5 0 73 44 266 628.5 0 266 45 157 628.5 0 157 46 286 628.5 0 286 47 49 628.5 0 49 48 35 628.5 0 35 49 50 628.5 0 50 50 46 628.5 0 46 51 83 628.5 0 83 52 84 628.5 0 84 53 108 628.5 0 108 54 48 628.5 0 48 55 46 628.5 0 46 56 83 628.5 0 83 57 112 628.5 0 112 58 503 628.5 0 503 59 415 628.5 0 415 60 336 628.5 0 336 61 60 628.5 0 60 62 51 628.5 0 51 63 43 628.5 0 43 64 36 628.5 0 36 65 36 628.5 0 36 66 53 628.5 0 53 67 57 628.5 0 57 68 64 628.5 0 64 69 46 628.5 0 46 70 40 628.5 0 40 71 49 628.5 0 49 72 49 628.5 0 49 73 71 628.5 0 71 74 53 628.5 0 53 75 92 628.5 0 92 76 69 628.5 0 69 77 82 628.5 0 82 78 51 628.5 0 51 79 44 628.5 0 44 80 64 628.5 0 64 81 47 628.5 0 47 82 67 628.5 0 67 83 77 628.5 0 77 84 66 628.5 0 66 85 57 628.5 0 57 86 60 628.5 0 60 87 51 628.5 0 51 88 68 628.5 0 68 89 60 628.5 0 60 90 70 628.5 0 70 91 114 628.5 0 114 92 129 628.5 0 129 93 187 628.5 0 187 94 88 628.5 0 88 95 66 628.5 0 66 96 62 628.5 0 62 97 110 628.5 0 110 98 67 628.5 0 67 99 80 628.5 0 80 100 60 628.5 0 60 101 87 628.5 0 87 102 80 628.5 0 80 103 63 628.5 0 63 104 84 628.5 0 84 105 119 628.5 0 119 106 713 628.5 0 713 107 423 628.5 0 423 108 613 628.5 0 613 109 94 628.5 0 94 110 79 628.5 0 79 111 78 628.5 0 78 112 90 628.5 0 90 113 75 628.5 0 75 114 526 628.5 0 526 115 660 628.5 0 660 116 546 628.5 0 546 117 87 628.5 0 87 118 59 628.5 0 59 119 63 628.5 0 63 120 44 628.5 0 44 121 64 628.5 0 64 122 72 628.5 0 72 123 84 628.5 0 84 124 109 628.5 0 109 125 85 628.5 0 85 126 69 628.5 0 69 127 88 628.5 0 88 128 386 628.5 0 386 129 270 628.5 0 270 130 239 628.5 0 239 131 92 628.5 0 92 132 97 628.5 0 97 133 79 628.5 0 79 134 194 628.5 0 194 135 171 628.5 0 171 136 423 628.5 0 423 137 338 628.5 0 338 138 246 628.5 0 246 139 167 628.5 0 167 140 439 628.5 0 439 141 427 628.5 0 427 142 595 628.5 0 595 143 939 628.5 0 939 144 103 628.5 0 103 145 119 628.5 0 119 146 74 628.5 0 74 147 83 628.5 0 83 148 1067 628.5 0 1067 149 731 628.5 0 731 150 848 628.5 0 848 151 64 628.5 0 64 152 46 628.5 0 46 153 67 628.5 0 67 154 93 628.5 0 93 155 431 628.5 0 431 156 243 628.5 0 243 157 381 628.5 0 381 158 121 628.5 0 121 159 53 628.5 0 53 160 62 628.5 0 62 161 107 628.5 0 107 162 105 628.5 0 105 163 374 628.5 0 374 164 342 628.5 0 342 165 499 628.5 0 499 166 83 628.5 0 83 167 46 628.5 0 46 168 89 628.5 0 89 169 57 628.5 0 57 170 97 628.5 0 97 171 18 628.5 0 18 172 29 628.5 0 29 173 42 628.5 0 42 174 45 628.5 0 45 175 47 628.5 0 47 176 49 628.5 0 49 177 45 628.5 0 45 178 56 628.5 0 56 179 85 628.5 0 85 180 309 628.5 0 309 181 156 628.5 0 156 182 190 628.5 0 190 183 85 628.5 0 85 184 68 628.5 0 68 185 95 628.5 0 95 186 96 628.5 0 96 187 42 628.5 0 42 188 69 628.5 0 69 189 54 628.5 0 54 190 57 628.5 0 57 191 76 628.5 0 76 192 50 628.5 0 50 193 79 628.5 0 79 194 45 628.5 0 45 195 58 628.5 0 58 196 63 628.5 0 63 197 56 628.5 0 56 198 59 628.5 0 59 199 53 628.5 0 53 200 53 628.5 0 53 201 67 628.5 0 67 202 339 628.5 0 339 203 169 628.5 0 169 204 156 628.5 0 156 205 44 628.5 0 44 206 57 628.5 0 57 207 42 628.5 0 42 208 68 628.5 0 68 209 78 628.5 0 78 210 54 628.5 0 54 211 63 628.5 0 63 212 52 628.5 0 52 213 101 628.5 0 101 214 122 628.5 0 122 215 125 628.5 0 125 216 119 628.5 0 119 217 77 628.5 0 77 218 61 628.5 0 61 219 50 628.5 0 50 220 54 628.5 0 54 221 50 628.5 0 50 222 67 628.5 0 67 223 88 628.5 0 88 224 73 628.5 0 73 225 62 628.5 0 62 226 85 628.5 0 85 227 56 628.5 0 56 228 77 628.5 0 77 229 52 628.5 0 52 230 56 628.5 0 56 231 71 628.5 0 71 232 94 628.5 0 94 233 153 628.5 0 153 234 200 628.5 0 200 235 138 628.5 0 138 236 95 628.5 0 95 237 51 628.5 0 51 238 54 628.5 0 54 239 71 628.5 0 71 240 58 628.5 0 58 241 63 628.5 0 63 242 95 628.5 0 95 243 90 628.5 0 90 244 121 628.5 0 121 245 77 628.5 0 77 246 58 628.5 0 58 247 70 628.5 0 70 248 75 628.5 0 75 249 78 628.5 0 78 250 63 628.5 0 63 251 92 628.5 0 92 252 102 628.5 0 102 253 145 628.5 0 145 254 87 628.5 0 87 255 172 628.5 0 172 256 58 628.5 0 58 257 74 628.5 0 74 258 85 628.5 0 85 259 66 628.5 0 66 260 87 628.5 0 87 261 214 628.5 0 214 262 147 628.5 0 147 263 141 628.5 0 141 264 101 628.5 0 101 265 94 628.5 0 94 266 82 628.5 0 82 267 93 628.5 0 93 268 85 628.5 0 85 269 123 628.5 0 123 270 133 628.5 0 133 271 497 628.5 0 497 272 402 628.5 0 402 273 259 628.5 0 259 274 97 628.5 0 97 275 92 628.5 0 92 276 83 628.5 0 83 277 84 628.5 0 84 278 136 628.5 0 136 279 189 628.5 0 189 280 147 628.5 0 147 281 94 628.5 0 94 282 80 628.5 0 80 283 132 628.5 0 132 284 135 628.5 0 135 285 140 628.5 0 140 286 82 628.5 0 82 287 77 628.5 0 77 288 87 628.5 0 87 289 64 628.5 0 64 290 117 628.5 0 117 291 132 628.5 0 132 292 213 628.5 0 213 293 186 628.5 0 186 294 463 628.5 0 463 295 1786 628.5 0 1786 296 1329 628.5 0 1329 297 1318 628.5 0 1318 298 320 628.5 0 320 299 66 628.5 0 66 300 58 628.5 0 58 301 61 628.5 0 61 Finished in 5.39 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 115,993 Reads with adapters: 49,251 (42.5%) Reads that were too short: 172 (0.1%) Reads written (passing filters): 49,079 (42.3%) Total basepairs processed: 31,090,253 bp Total written (filtered): 13,547,987 bp (43.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49251 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 36 0.0 3 0 0 15 21 16 347 0.0 3 0 270 48 29 17 47809 0.0 3 43390 3559 673 187 18 157 0.0 3 6 114 19 18 19 190 0.0 3 8 5 93 84 20 219 0.0 3 1 1 1 216 21 3 0.0 3 0 0 0 3 22 1 0.0 3 1 23 2 0.0 3 1 0 0 1 26 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 30 10 0.0 3 0 0 0 10 32 1 0.0 3 0 0 0 1 33 5 0.0 3 0 0 1 4 38 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 77 0.0 3 0 0 1 76 44 1 0.0 3 0 0 0 1 45 5 0.0 3 0 0 0 5 46 13 0.0 3 0 0 0 13 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 51 9 0.0 3 0 0 0 9 54 6 0.0 3 0 0 0 6 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 1 1 59 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 63 2 0.0 3 0 0 1 1 64 8 0.0 3 0 0 0 8 65 1 0.0 3 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 56 0.0 3 0 0 3 53 70 21 0.0 3 0 0 1 20 71 2 0.0 3 0 0 0 2 72 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 3 0.0 3 0 0 0 3 81 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 0 4 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 0 4 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 21 0.0 3 0 0 0 21 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 5 0.0 3 0 0 0 5 117 2 0.0 3 0 0 0 2 120 5 0.0 3 0 0 0 5 121 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 0 4 129 1 0.0 3 0 0 1 130 4 0.0 3 0 0 0 4 132 2 0.0 3 0 0 0 2 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 6 0.0 3 0 0 1 5 150 5 0.0 3 0 0 0 5 151 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 168 3 0.0 3 0 0 0 3 172 2 0.0 3 0 0 0 2 173 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 3 0.0 3 0 0 0 3 181 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 9 0.0 3 0 0 0 9 195 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 27 0.0 3 0 0 1 26 218 8 0.0 3 0 0 0 8 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 243 2 0.0 3 0 0 0 2 247 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 7 0.0 3 0 0 0 7 281 6 0.0 3 0 0 1 5 282 2 0.0 3 0 0 0 2 283 3 0.0 3 0 0 0 3 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.71 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 49,079 Reads with adapters: 233 (0.5%) Reads written (passing filters): 49,079 (100.0%) Total basepairs processed: 13,547,987 bp Total written (filtered): 13,547,259 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 233 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.2% C: 42.1% G: 24.0% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 211 766.9 0 211 4 15 191.7 0 15 5 7 47.9 0 7