This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.46 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 118,791 (4.6%) Reads that were too short: 6,669 (0.3%) Reads written (passing filters): 112,122 (4.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 30,975,013 bp (4.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 118791 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 842 628.5 0 842 7 49484 628.5 0 49484 8 1860 628.5 0 1860 9 43239 628.5 0 43239 10 58 628.5 0 58 11 29 628.5 0 29 12 41 628.5 0 41 13 35 628.5 0 35 14 24 628.5 0 24 15 20 628.5 0 20 16 40 628.5 0 40 17 33 628.5 0 33 18 26 628.5 0 26 19 25 628.5 0 25 20 23 628.5 0 23 21 19 628.5 0 19 22 24 628.5 0 24 23 27 628.5 0 27 24 20 628.5 0 20 25 36 628.5 0 36 26 26 628.5 0 26 27 33 628.5 0 33 28 42 628.5 0 42 29 33 628.5 0 33 30 43 628.5 0 43 31 44 628.5 0 44 32 32 628.5 0 32 33 51 628.5 0 51 34 26 628.5 0 26 35 33 628.5 0 33 36 31 628.5 0 31 37 35 628.5 0 35 38 42 628.5 0 42 39 44 628.5 0 44 40 46 628.5 0 46 41 28 628.5 0 28 42 24 628.5 0 24 43 50 628.5 0 50 44 171 628.5 0 171 45 72 628.5 0 72 46 172 628.5 0 172 47 27 628.5 0 27 48 28 628.5 0 28 49 47 628.5 0 47 50 26 628.5 0 26 51 48 628.5 0 48 52 36 628.5 0 36 53 30 628.5 0 30 54 37 628.5 0 37 55 35 628.5 0 35 56 44 628.5 0 44 57 33 628.5 0 33 58 68 628.5 0 68 59 63 628.5 0 63 60 42 628.5 0 42 61 35 628.5 0 35 62 35 628.5 0 35 63 29 628.5 0 29 64 31 628.5 0 31 65 28 628.5 0 28 66 40 628.5 0 40 67 36 628.5 0 36 68 55 628.5 0 55 69 26 628.5 0 26 70 35 628.5 0 35 71 35 628.5 0 35 72 26 628.5 0 26 73 40 628.5 0 40 74 38 628.5 0 38 75 56 628.5 0 56 76 33 628.5 0 33 77 45 628.5 0 45 78 33 628.5 0 33 79 40 628.5 0 40 80 26 628.5 0 26 81 42 628.5 0 42 82 41 628.5 0 41 83 56 628.5 0 56 84 50 628.5 0 50 85 48 628.5 0 48 86 39 628.5 0 39 87 33 628.5 0 33 88 30 628.5 0 30 89 41 628.5 0 41 90 51 628.5 0 51 91 76 628.5 0 76 92 72 628.5 0 72 93 55 628.5 0 55 94 50 628.5 0 50 95 33 628.5 0 33 96 42 628.5 0 42 97 50 628.5 0 50 98 54 628.5 0 54 99 35 628.5 0 35 100 54 628.5 0 54 101 61 628.5 0 61 102 45 628.5 0 45 103 68 628.5 0 68 104 64 628.5 0 64 105 68 628.5 0 68 106 465 628.5 0 465 107 279 628.5 0 279 108 312 628.5 0 312 109 67 628.5 0 67 110 54 628.5 0 54 111 65 628.5 0 65 112 58 628.5 0 58 113 59 628.5 0 59 114 600 628.5 0 600 115 832 628.5 0 832 116 730 628.5 0 730 117 54 628.5 0 54 118 42 628.5 0 42 119 42 628.5 0 42 120 39 628.5 0 39 121 54 628.5 0 54 122 44 628.5 0 44 123 65 628.5 0 65 124 65 628.5 0 65 125 48 628.5 0 48 126 57 628.5 0 57 127 40 628.5 0 40 128 119 628.5 0 119 129 78 628.5 0 78 130 99 628.5 0 99 131 56 628.5 0 56 132 62 628.5 0 62 133 69 628.5 0 69 134 109 628.5 0 109 135 106 628.5 0 106 136 241 628.5 0 241 137 333 628.5 0 333 138 207 628.5 0 207 139 105 628.5 0 105 140 390 628.5 0 390 141 299 628.5 0 299 142 245 628.5 0 245 143 301 628.5 0 301 144 47 628.5 0 47 145 51 628.5 0 51 146 55 628.5 0 55 147 51 628.5 0 51 148 1047 628.5 0 1047 149 580 628.5 0 580 150 771 628.5 0 771 151 36 628.5 0 36 152 35 628.5 0 35 153 44 628.5 0 44 154 32 628.5 0 32 155 166 628.5 0 166 156 151 628.5 0 151 157 156 628.5 0 156 158 87 628.5 0 87 159 45 628.5 0 45 160 51 628.5 0 51 161 82 628.5 0 82 162 68 628.5 0 68 163 207 628.5 0 207 164 157 628.5 0 157 165 246 628.5 0 246 166 63 628.5 0 63 167 39 628.5 0 39 168 41 628.5 0 41 169 43 628.5 0 43 170 39 628.5 0 39 171 30 628.5 0 30 172 26 628.5 0 26 173 28 628.5 0 28 174 27 628.5 0 27 175 38 628.5 0 38 176 24 628.5 0 24 177 27 628.5 0 27 178 30 628.5 0 30 179 57 628.5 0 57 180 104 628.5 0 104 181 85 628.5 0 85 182 86 628.5 0 86 183 47 628.5 0 47 184 30 628.5 0 30 185 43 628.5 0 43 186 44 628.5 0 44 187 29 628.5 0 29 188 46 628.5 0 46 189 44 628.5 0 44 190 28 628.5 0 28 191 37 628.5 0 37 192 51 628.5 0 51 193 49 628.5 0 49 194 30 628.5 0 30 195 34 628.5 0 34 196 33 628.5 0 33 197 30 628.5 0 30 198 44 628.5 0 44 199 34 628.5 0 34 200 48 628.5 0 48 201 52 628.5 0 52 202 176 628.5 0 176 203 93 628.5 0 93 204 83 628.5 0 83 205 33 628.5 0 33 206 33 628.5 0 33 207 27 628.5 0 27 208 34 628.5 0 34 209 60 628.5 0 60 210 41 628.5 0 41 211 27 628.5 0 27 212 42 628.5 0 42 213 64 628.5 0 64 214 46 628.5 0 46 215 54 628.5 0 54 216 67 628.5 0 67 217 47 628.5 0 47 218 43 628.5 0 43 219 26 628.5 0 26 220 28 628.5 0 28 221 38 628.5 0 38 222 47 628.5 0 47 223 64 628.5 0 64 224 46 628.5 0 46 225 38 628.5 0 38 226 45 628.5 0 45 227 38 628.5 0 38 228 39 628.5 0 39 229 44 628.5 0 44 230 43 628.5 0 43 231 34 628.5 0 34 232 54 628.5 0 54 233 65 628.5 0 65 234 55 628.5 0 55 235 65 628.5 0 65 236 58 628.5 0 58 237 49 628.5 0 49 238 48 628.5 0 48 239 51 628.5 0 51 240 29 628.5 0 29 241 50 628.5 0 50 242 42 628.5 0 42 243 41 628.5 0 41 244 63 628.5 0 63 245 45 628.5 0 45 246 33 628.5 0 33 247 48 628.5 0 48 248 66 628.5 0 66 249 67 628.5 0 67 250 45 628.5 0 45 251 33 628.5 0 33 252 84 628.5 0 84 253 65 628.5 0 65 254 56 628.5 0 56 255 58 628.5 0 58 256 51 628.5 0 51 257 56 628.5 0 56 258 45 628.5 0 45 259 35 628.5 0 35 260 51 628.5 0 51 261 72 628.5 0 72 262 79 628.5 0 79 263 71 628.5 0 71 264 40 628.5 0 40 265 56 628.5 0 56 266 59 628.5 0 59 267 42 628.5 0 42 268 59 628.5 0 59 269 47 628.5 0 47 270 67 628.5 0 67 271 165 628.5 0 165 272 171 628.5 0 171 273 132 628.5 0 132 274 79 628.5 0 79 275 87 628.5 0 87 276 56 628.5 0 56 277 49 628.5 0 49 278 78 628.5 0 78 279 78 628.5 0 78 280 89 628.5 0 89 281 69 628.5 0 69 282 44 628.5 0 44 283 53 628.5 0 53 284 72 628.5 0 72 285 57 628.5 0 57 286 49 628.5 0 49 287 34 628.5 0 34 288 68 628.5 0 68 289 53 628.5 0 53 290 57 628.5 0 57 291 57 628.5 0 57 292 89 628.5 0 89 293 78 628.5 0 78 294 125 628.5 0 125 295 329 628.5 0 329 296 273 628.5 0 273 297 325 628.5 0 325 298 105 628.5 0 105 299 49 628.5 0 49 300 43 628.5 0 43 301 56 628.5 0 56 Finished in 5.73 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 112,122 Reads with adapters: 46,049 (41.1%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 46,021 (41.0%) Total basepairs processed: 30,975,013 bp Total written (filtered): 12,485,174 bp (40.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 46049 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 20 0.0 3 0 0 19 1 19 390 0.0 3 0 386 4 20 45102 0.0 4 44394 688 18 1 1 21 118 0.0 4 22 94 2 22 22 0.0 4 16 0 6 23 28 0.0 4 0 0 0 20 8 27 2 0.0 4 0 0 0 0 2 43 7 0.0 4 0 0 0 0 7 83 3 0.0 4 0 0 0 0 3 99 1 0.0 4 0 1 110 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 318 0.0 4 0 0 0 0 318 128 3 0.0 4 0 0 0 0 3 130 1 0.0 4 1 141 1 0.0 4 1 172 1 0.0 4 1 173 1 0.0 4 1 199 1 0.0 4 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 1 0 1 229 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 237 3 0.0 4 0 0 0 0 3 238 3 0.0 4 2 0 1 239 1 0.0 4 0 1 240 3 0.0 4 2 0 1 246 1 0.0 4 0 1 265 1 0.0 4 0 0 0 0 1 276 1 0.0 4 1 283 2 0.0 4 1 0 0 1 285 1 0.0 4 0 1 286 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.64 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 46,021 Reads with adapters: 6,829 (14.8%) Reads written (passing filters): 46,021 (100.0%) Total basepairs processed: 12,485,174 bp Total written (filtered): 12,363,105 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6829 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 3.0% G: 93.8% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 719.1 0 12 4 12 179.8 0 12 5 4 44.9 0 4 6 3 11.2 0 3 7 1 2.8 0 1 8 3 0.7 0 3 9 2816 0.2 0 80 2736 10 2627 0.0 1 96 2531 11 1082 0.0 1 12 1070 12 4 0.0 1 0 4 13 3 0.0 1 0 3 18 7 0.0 1 6 1 19 15 0.0 1 12 3 22 7 0.0 1 5 2 36 1 0.0 1 0 1 71 1 0.0 1 1 81 2 0.0 1 2 90 1 0.0 1 0 1 105 1 0.0 1 1 106 1 0.0 1 1 107 2 0.0 1 1 1 108 2 0.0 1 0 2 110 1 0.0 1 1 119 1 0.0 1 1 150 3 0.0 1 0 3 151 1 0.0 1 0 1 156 1 0.0 1 1 177 1 0.0 1 1 215 26 0.0 1 25 1 217 1 0.0 1 1 218 1 0.0 1 1 238 3 0.0 1 3 241 1 0.0 1 1 243 1 0.0 1 1 266 1 0.0 1 1 267 163 0.0 1 153 10 268 9 0.0 1 9 269 1 0.0 1 1 270 3 0.0 1 3 271 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.